What is the basic principle of ion-exchange chromatography?

What is the basic principle of ion-exchange chromatography?
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Answer :

Here, the separation is based on electrostatic attraction between charged molecules to oppositely charged groups on the ion exchange resins.
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The following technique makes use of the difference in net charges of proteins at a given pH: (A) Thin layer chromatography (B) Ion exchange chromatography (C) High performance liquid chromatography (D) Paper chromatography

Description : The following technique makes use of the difference in net charges of proteins at a given pH: (A) Thin layer chromatography (B) Ion exchange chromatography (C) High performance liquid chromatography (D) Paper chromatography

Last Answer : Answer : B

The technique for purification of proteins that can be made specific for a given protein is (A) Gel filtration chromotography (B) Ion exchange chromatography (C) Electrophoresis (D) Affinity chromatography

Description : The technique for purification of proteins that can be made specific for a given protein is (A) Gel filtration chromotography (B) Ion exchange chromatography (C) Electrophoresis (D) Affinity chromatography

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What is the principle of gel filtration chromatography?

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Last Answer : The separation is effected on the basis of the size of the molecules. It is otherwise called molecular sieving.

What is the principle of partition chromatography?

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Last Answer : the components of the mixture to be separated are partitioned between the two phases depending on the partition co-efficient (solubility) of the particular substances.

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Explain the principle of paper chromatography.

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Chloride shift is (A) H ions leaving the RBC in exchange of Cl- (B) Cl– leaving the RBC in exchange of bicarbonate (C) Bicarbonate ion returns to plasma and exchanged with chloride which shifts into the cell (D) Carbonic acid to the plasma

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Presence of arginine can be detected by (A) Sakaguchi reaction (B) Million-Nasse reaction (C) Hopkins-Cole reaction (D) Gas chromatography

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Frequently employed materials for the adsorption chromatography of proteins include (A) High capacity supporting gel (B) Starch blocks (C) Calcium phosphate gel alumina gel and hydroxy apatite (D) All of these

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Last Answer : Answer : C

The sorting out of molecules according to size and shape may be adapted to protein purification in this technique: (A) Adsorption chromatography (B) Gel filtration chromatography (C) Paper chromatography (D) None of these

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The movement of charged particles towards one of the electrodes under the influence of electrical current is (A) Gel filtration (B) Molecular sieving (C) Gas liquid chromatography (D) Electrophoresis

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This technique takes the advantage of the fact that each protein has different pH at which it is electrically neutral i.e., its isoelectric pH: (A) Isoelectric focussing (B) Immunoel Ectro Phoresis (C) Chromatography (D) HPLC

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A sharp moving boundary is obtained between the pure solvent and solute containing layer in (A) Chromatography (B) Immuno Reactivity (C) Ultra Centrifugation (D) Solubility curve

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Transports of mineral by ion exchange. -Biology

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Last Answer : answer:

The ease of adsorption of the hydrated alkali metal ions on ion-exchange resins follows the order:

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`RH_(2)` ( ion exchange resin) can replace `Ca^(2+)`d in hard water as. `RH_(2)+Ca^(2+)rarrRCa+2H^(+)` `1 "litre"` of hard water passing through `RH_(

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When any solution passess through a cation exchange resin that is in acidic form, H ion of the resin is replaced by cations of the solution. A solutio

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Iron and manganese present as pollutants in water cannot be re-moved by - (1) oxidation followed by settling and filtration (2) chlorination (3) ion exchange process (4) lime soda process or manganese zeolite process

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Last Answer : (2) chlorination Explanation: Water chlorination is the process of adding chlorine or hypochlorite to water. This method is used to kill certain bacteria and other microbes in tap water as chlorine is ... is used to prevent the spread of waterborne diseases such as cholera, dysentery, typhoid etc.

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Non sulphonated hard bakelites are not used for making (A) Ion-exchange resins (B) Fountain pen barrels (C) Formica table tops (D) Combs

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Human urine is usually acidic because (a) potassium and sodium exchange generates acidity (b) hydrogen ions are actively secreted into the filtrate (c) the sodium transporter exchanges one hydrogen ion for each sodium ion, in peritubular capillaries (d) excreted plasma proteins are acidic.

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One of the methods of purification of leach liquor is ion exchange, which involves (A) Exchange between two liquid phases (B) Exchange between a gaseous phase and a liquid phase (C) Exchange between a liquid phase and an organic resin phase (D) Exchange between a solid phase and a gas phase

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Consider the following statements: 1. Carbonate hardness is due to bicarbonates. 2. Non-carbonate hardness is due to sulphates and chlorides of Ca and Mg. 3. Both the hardnesses can be removed by lime-soda method. 4. Both the hardnesses can be removed by ion-exchange method. Which of these statements are correct? (a) 1, 2 & 3 only (b) 1, 2 & 4 only (c) 2, 3 & 4 only (d) 1, 2, 3 & 4

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An adsorbant a). Activated carbon b). lignin byproducts c). ion exchange resins d). All

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Iron and manganese present as pollutants in water cannot be removed by_____ (1) oxidation followed by settling and filtration (2) chlorination (3) ion exchange process (4) lime soda process or manganese zeolite process

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“Rate of softening of water in Ion Exchange method is high,” Give reason.

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