Description : The sorting out of molecules according to size and shape may be adapted to protein purification in this technique: (A) Adsorption chromatography (B) Gel filtration chromatography (C) Paper chromatography (D) None of these
Last Answer : Answer : B
Description : The movement of charged particles towards one of the electrodes under the influence of electrical current is (A) Gel filtration (B) Molecular sieving (C) Gas liquid chromatography (D) Electrophoresis
Last Answer : Answer : D
Description : The following technique makes use of the difference in net charges of proteins at a given pH: (A) Thin layer chromatography (B) Ion exchange chromatography (C) High performance liquid chromatography (D) Paper chromatography
Description : What is the principle of gel filtration chromatography?
Last Answer : The separation is effected on the basis of the size of the molecules. It is otherwise called molecular sieving.
Description : Primary structure of proteins can be determined by the use of (A) Electrophoresis (B) Chromatography (C) Ninhydrin (D) Sanger’s reagent
Description : Frequently employed materials for the adsorption chromatography of proteins include (A) High capacity supporting gel (B) Starch blocks (C) Calcium phosphate gel alumina gel and hydroxy apatite (D) All of these
Last Answer : Answer : C
Description : Which of the following techniques is used to separate proteins based upon differences in their mass? (A) Isoelectric focusing (B) Dialysis (C) SDS-gel Electrophoresis (D) Western blotting
Description : If two persons with AB' blood group marry and have sufficiently large number of children, these children could be classified as A' blood group: AB' blood group : B' blood group in 1 ... in an example of (a) partial dominance (b) complete dominance (c) codominance (d) incomplete dominance.
Last Answer : (c) codominance
Description : What is the basic principle of ion-exchange chromatography?
Last Answer : Here, the separation is based on electrostatic attraction between charged molecules to oppositely charged groups on the ion exchange resins.
Description : This technique takes the advantage of the fact that each protein has different pH at which it is electrically neutral i.e., its isoelectric pH: (A) Isoelectric focussing (B) Immunoel Ectro Phoresis (C) Chromatography (D) HPLC
Last Answer : Answer : A
Description : The antibodies raised against a pure protein will show only one sharp spike on this technique: (A) Solubility curve (B) Solvent precipitation (C) Molecular weight determination (D) Immuno electrophoresis
Description : Give an example of affinity chromatography.
Last Answer : Separation and quantitation of glycated hemoglobin.
Description : Give the principle of affinity chromatography.
Last Answer : The technique is based on the high affinity of specific proteins for specific chemical groups.
Description : One of the methods of purification of leach liquor is ion exchange, which involves (A) Exchange between two liquid phases (B) Exchange between a gaseous phase and a liquid phase (C) Exchange between a liquid phase and an organic resin phase (D) Exchange between a solid phase and a gas phase
Last Answer : (C) Exchange between a liquid phase and an organic resin phase
Description : Given below are four statements pertaining to separation of DNA fragments using gel electrophoresis. Identify the incorrect statements. (i) DNA is negatively charged molecule and so it is loaded on gel towards the anode terminal. (ii) DNA ... (iii) (c) (ii), (iii) and (iv) (d) (i), (ii) and (iv)
Last Answer : (d) (i), (ii) and (iv)
Description : Iron and manganese present as pollutants in water cannot be re-moved by - (1) oxidation followed by settling and filtration (2) chlorination (3) ion exchange process (4) lime soda process or manganese zeolite process
Last Answer : (2) chlorination Explanation: Water chlorination is the process of adding chlorine or hypochlorite to water. This method is used to kill certain bacteria and other microbes in tap water as chlorine is ... is used to prevent the spread of waterborne diseases such as cholera, dysentery, typhoid etc.
Description : Radioactive substances present in the polluted water stream can be removed by (A) Biological oxygen treatment (B) Coagulation and filtration (C) Adsorption in ion exchange materials (D) None of these
Last Answer : (C) Adsorption in ion exchange materials
Description : Iron & manganese present as pollutant in water cannot be removed by (A) Ion exchange process (B) Oxidation followed by settling & filtration (C) Lime soda process or manganese zeolite process (D) Chlorination
Last Answer : (D) Chlorination
Description : Iron and manganese present as pollutants in water cannot be removed by_____ (1) oxidation followed by settling and filtration (2) chlorination (3) ion exchange process (4) lime soda process or manganese zeolite process
Last Answer : chlorination
Description : If a metal has low oxygen affinity then the purification of metal may be carried out by :
Last Answer : If a metal has low oxygen affinity then the purification of metal may be carried out by : A. liquation B. distillation C. zone refining D. cupellation
Description : If the impurity in a metal has a greater affinity for oxygen and is more easily oxidised than the metal, then the purification of metal may be carried
Last Answer : If the impurity in a metal has a greater affinity for oxygen and is more easily oxidised than ... B. electrorefining C. Zone refining D. POILING
Description : A process in which substances are separated throug differences in the rates at which the components migrate is called: w) filtration x) chromatography y) elution z) titration
Last Answer : ANSWER: X -- CHROMATOGRAPHY
Description : How long can I leave this 1% agarose gel out in room temperature before electrophoresis?
Last Answer : From surfing around, it looks like the gel will last several weeks at room temperature without drying out. I couldn’t find anything that indicated that which dye is used matters, but I doubt it.
Description : Why does DNA move towards the anode in gel electrophoresis? -Biology
Last Answer : answer:
Description : What is gel electrophoresis? -Biology
Description : What is agarose gel electrophoresis? -Biology
Description : What is agarose gel electrophoresis used for? -Biology
Description : What does agarose gel electrophoresis do? -Biology
Description : What is gel electrophoresis used for? -Biology
Description : How does gel electrophoresis separate DNA fragments?
Last Answer : There is a specific density of gel used in the electrophoresis.The DNA is placed in a well, and then electrical charge is used topull the DNA through the gel. Because spliced DNA is ... taxi cab, which is larger, movesslower through the traffic. The bicycle which is smaller, movesquicker.
Description : Why can gel electrophoresis separate dna fragments?
Last Answer : Electrophoresis enables you to distinguish DNA fragments of different lengths. DNA is negatively charged, therefore, when an electric current is applied to the gel, DNA will migrate towards the positively ... marker, you can work out the approximate length of the DNA fragments in the samples.
Description : Write the importance of gel electrophoresis during DNA finger print technology.
Last Answer : Gel electrophoresis helps to break down the DNA of an individual into short fragments from which a DNA finger print can be obtained.
Description : What is gel electrophoresis?
Last Answer : The technique of breaking down the DNA of individual into short segments using specific enzymes is called gel electrophoresis.
Description : Gel electrophoresis is used for (a) construction of recombinant DNA by joining with cloning vectors (b) isolation of DNA molecules (c) cutting of DNA into fragments (d) separation of DNA fragments according to their size.
Last Answer : (d) separation of DNA fragments according to their size.
Description : Agarose extracted from sea weeds is used in (a) spectrophotometry (b) tissue culture (c) PCR (d) gel electrophoresis
Last Answer : (d) gel electrophoresis.
Description : What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis ? (a) The smaller the fragment size, the farther it moves. (b) Positively charged fragments move to farther end. ... charged fragments do not move. (d) The larger the fragment size, the farther it moves.
Last Answer : (a) The smaller the fragment size, the farther it moves
Description : In gel electrophoresis, separated DNA fragments can be visualized with the help of (a) acetocarmine in bright blue light (b) ethidium bromide in UV radiation (c) acetocarmine in UV radiation (d) ethidium bromide in infrared radiation.
Last Answer : (b) ethidium bromide in UV radiation
Description : 6) In agarose gel electrophoresis, DNA molecules are separated on the basis of their- a)charge only b)size only c)charge to size ratio d)all of the above
Last Answer : d)all of the above
Description : 11. Gel electrophoresis is used for a) Construction of recombinant DNA by joining with cloning vectors b) Isolation of DNA molecules c) Cutting of DNA into fragments d)Separation of DNA fragments according to their size
Last Answer : d)Separation of DNA fragments according to their size
Description : What is the criterion for DN A fragments movement on agarose gel during gel electrophoresis ? (1) The smaller the fragment size, the farther it moves (2) Positively charged fragments move to farther ... Negatively charged fragments do not move (4) The larger the fragment size, the farther it moves
Last Answer : (1) The smaller the fragment size, the farther it moves
Description : Plasma proteins are isolated by (A) Salting out (B) Electrophoresis (C) Flourimetry (D) Both (A) and (B)
Description : Human urine is usually acidic because (a) potassium and sodium exchange generates acidity (b) hydrogen ions are actively secreted into the filtrate (c) the sodium transporter exchanges one hydrogen ion for each sodium ion, in peritubular capillaries (d) excreted plasma proteins are acidic.
Last Answer : (b) hydrogen ions are actively secreted into the filtrate
Description : Plasma protein can be separated by (A) Salting out with (NH4)2SO4 (B) Ultracentrifugation (C) Immuno electrophoresis (D) All of these
Description : Radioactive elements can be traced out in living cell through a technique called – (1) Centrifugation (2) Chromatography (3) Auto radiography (4) All the above
Last Answer : (3) Auto radiography Explanation: Radioactive elements can be traced out in living cell through a technique called Autoradiography.
Description : The basis of the technique of chromatography for separating components of a mixture is? A. the differing movement of particles of different mass in an electrical field B. the interaction of the ... infrared radiation by the components. D. the deflection of charged particles in a magnetic field.
Last Answer : the interaction of the components with a stationary and a mobile phases
Description : Radioactive elements can be traced out in living cell through a technique called (1) Centrifugation (2) Chromatography (3) Auto radiography (4) All the above
Last Answer : Auto radiography
Description : An analysis of chromosomal DNA using the Southern hybridization technique does not use (a) electrophoresis (b) blotting (c) autoradiography (d) PCR.
Last Answer : (d) PCR.
Description : A specific fructokinase present in liver has a very high affinity for its substrate because (A) Km for fructose is very high (B) Km for fructose is very low (C) Activity is affected by fasting (D) Activity is affected by insulin