This technique takes the advantage of the fact that each protein has different pH at which it is electrically neutral i.e., its isoelectric pH: (A) Isoelectric focussing (B) Immunoel Ectro Phoresis (C) Chromatography (D) HPLC

1 Answer

Answer :

Answer : A

Related questions

Description : Isoelectric pH is that pH at which protein is electrically: (A) Neutral (B) Anionic (C) Cationic (D) None of these

Last Answer : Answer : A

Description : The solubility of most proteins is lowered at high salt concentrations is called as (A) Salting in process (B) Salting out process (C) Isoelectric focussing(D) None of these

Last Answer : Answer : B

Description : The following technique makes use of the difference in net charges of proteins at a given pH: (A) Thin layer chromatography (B) Ion exchange chromatography (C) High performance liquid chromatography (D) Paper chromatography

Last Answer : Answer : B

Description : HPLC is an abbreviation for? A. High Profit Liquid Chromatography B. High Pressure Liquid Chromatography C. Higher Performance Low Chromatography D. Higher Profit Low Chromatography

Last Answer : High Pressure Liquid Chromatography

Description : The sorting out of molecules according to size and shape may be adapted to protein purification in this technique: (A) Adsorption chromatography (B) Gel filtration chromatography (C) Paper chromatography (D) None of these

Last Answer : Answer : B

Description : The technique for purification of proteins that can be made specific for a given protein is (A) Gel filtration chromotography (B) Ion exchange chromatography (C) Electrophoresis (D) Affinity chromatography

Last Answer : Answer : D

Description : The isoelectric point of a protein is (a) the pH at which the protein molecule has no charges on its surface. (b) the pH at which a protein in solution has an equal number of positive and negative charges. (c) the electric charge under isothermal conditions. (b) None of these

Last Answer : the pH at which a protein in solution has an equal number of positive and negative charges

Description : In the genetic disorder of cystinuria, the patient excretes large quantities of cystine in their urine and its low solubility causes crystalline cystine to precipitate as stones in kidneys. The remedy ... C) NaHCO3 prevents stone formation by hydrolysis of cystine to cysteine (D) None of these

Last Answer : Answer : C

Description : Velocity maximum for an enzyme at half the substrate concentration gives (A) The molecular weight of the enzyme (B) Km value (C) Isoelectric pH (D) Pk value

Last Answer : Answer : B

Description : The pH at which an enzyme has maximum activity is known as (A) Isoelectric pH (B) Optimum pH (C) Low pH (D) High pH

Last Answer : Answer : B

Description : Isoelectric pH of an amino acid is that pH at which it has a (A) Positive charge (B) Negative charge (C) Nil net charge (D) None of these

Last Answer : Answer : C

Description : Isoelectric pH of an amino acid is that pH at which it has a (A) Positive charge (B) Negative charge (C) No charge (D) None of these

Last Answer : Answer : C

Description : Isoelectric pH of an amino acid is that pH at which it has a (A) Positive charge (B) Negative charge (C) No net charge (D) All of these

Last Answer : Answer : C

Description : At a pH below the isoelectric point, an amino acid exists as (A) Cation (B) Anion (C) Zwitterion (D) Undissociated molecule

Last Answer : Answer : A

Description : At isoelectric pH, an amino acid exists as (A) Anion (B) Cation (C) Zwitterion (D) None of these

Last Answer : Answer : C

Description : Since the pK values for aspartic acid are 2.0, 3.9 and 10.0, it follows that the isoelectric (pH) is (A) 3.0 (B) 3.9 (C) 5.9 (D) 6.0

Last Answer : Answer : A

Description : pH (isoelectric pH) of alanine is (A) 6.02 (B) 6.6 (C) 6.8 (D) 7.2

Last Answer : Answer : A

Description : Since the pK values for aspartic acid are 2.0, 3.9 and 10.0, it follows that the isoelectric (pH) is (A) 3.0 (B) 3.9 (C) 5.9 (D) 6.0

Last Answer : (A) 3.0

Description : pH (isoelectric pH) of alanine is (A) 6.02 (B) 6.6 (C) 6.8 (D) 7.2

Last Answer : (A) 6.02

Description : What is the advantage of TLC over paper chromatography? 

Last Answer : TLC needs lesser time, and separation is more effective.

Description : When a semiconductor is doped with a P-type impurity, each impurity atom will (A) acquire negative charge. (B) acquire positive charge. (C) remain electrically neutral. (D) give away one electron.

Last Answer : (A) acquire negative charge.

Description : The pH at which the amino acid shows no tendency to migrate when placed in an electric field is known as its : (a) Isoelectric point (b) Dipole moment (c) Iodine number (d) Wavelength

Last Answer : Isoelectric point

Description : The basis of the technique of chromatography for separating components of a mixture is? A. the differing movement of particles of different mass in an electrical field B. the interaction of the ... infrared radiation by the components. D. the deflection of charged particles in a magnetic field. 

Last Answer : the interaction of the components with a stationary and a mobile phases

Description : When nucleus of an electrically neutral atom undergoes a radioactive decay process, it will remain neutral after the decay if the process is (a) An `a

Last Answer : When nucleus of an electrically neutral atom undergoes a radioactive decay process, it will remain neutral ... a`gamma`-decay D. a K-capture process

Description : A good conductor while carrying current is - (1) alternately charged positive and negative (2) negatively charged (3) positively charged (4) electrically neutral

Last Answer : (2) negatively charged Explanation: Electrons carry current in a good conductor of electricity and they are negatively charged. This makes a good electrical conductor negatively charged.

Description : When one material is rubbed against the other, then it becomes electrically A. neutral B. charged C. positively charged D. negatively charged

Last Answer : charged

Description : Why an electrolyte in molten or fused state is electrically neutral?

Last Answer : Ans: Because the number of + ve charges and –ve charges are equal , so as a result an electrolyte appears to be neutral in nature.

Description : A good conductor while carrying current is (1) alternately charged positive and negative (2) negatively charged (3) positively charged (4) electrically neutral 

Last Answer : negatively charged

Description : A long, hollow conducting cylinder is kept coaxially inside another long, hollow conducting cylinder of larger radius. Both the cylinders are initially electrically neutral. (A) A potential ... difference appears between the two cylinders when same charge density is given to both the cylinders

Last Answer : (A) A potential difference appears between the two cylinders when a charge density is given to the inner cylinder

Description : Why atom is electrically neutral? 

Last Answer : Atom of an element is electrically neutral because it contains equal number of proton & electrons. In other words in an atom of an element number of positive charges (protons) are equal to number of ... (electrons) . Hence they cancel each other & as a result atom becomes charge-less or neutral. 

Description : Radioactive elements can be traced out in living cell through a technique called – (1) Centrifugation (2) Chromatography (3) Auto radiography (4) All the above

Last Answer : (3) Auto radiography Explanation: Radioactive elements can be traced out in living cell through a technique called Autoradiography.

Description : Radioactive elements can be traced out in living cell through a technique called (1) Centrifugation (2) Chromatography (3) Auto radiography (4) All the above

Last Answer : Auto radiography

Last Answer : EPROM EEPROM Flash EPROM Normalize cell size is 1. Normalize cell size is about 1 ... and floating gate, which is insulated by a dielectric material. NAND flash memory and NOR flash memory are the two types of flash memory.

Description : The Michaehis-Menten hypothesis: (A) Postulates the formation of an enzyme substrate complex (B) Enables us to calculate the isoelectric point of an enzyme (C) States that the ... independent of substrate concentration (D) States that the reaction rate is proportional to substrate concentration

Last Answer : Answer : A

Description : Isoelectric point of amino acids is used for (A) Crystallisation (B) Precipitation (C) Solubility (D) Reactivity

Last Answer : Answer : B

Description : Which of the following techniques is used to separate proteins based upon differences in their mass? (A) Isoelectric focusing (B) Dialysis (C) SDS-gel Electrophoresis (D) Western blotting

Last Answer : Answer : C

Description : Management by exception implies focussing attention on– (A) All variations (B) Normal variations (C) Abnormal variations (D) Randomly selected variations

Last Answer : Answer: Abnormal variations

Description : An internal focussing type surveying telescope, may be focussed by the movement of  (A) Objective glass of the telescope  (B) Convex-lens in the telescope  (C) Concave lens in the telescope  (D) Plano-convex lens in the telescope

Last Answer : (C) Concave lens in the telescope 

Description : Removal of parallax, may be achieved by focussing  (A) The objective  (B) The eye-piece  (C) The objective and the eye-piece  (D) None of these 

Last Answer : (C) The objective and the eye-piece 

Description : In a telescope the object glass of focal length 14 cm, is located at 20 cm from the diaphragm. The focussing lens is midway between them when a staff 16.50 m away is focussed. The focal length of the focussing lens, is (A) 5.24 cm (B) 6.24 cm (C) 7.24 cm (D) 8.24 cm

Last Answer : (C) 7.24 cm

Description : Maximum enzyme activity is observed at (A) Acidic pH (B) Neutral pH (C) Basic pH (D) Optimum pH

Last Answer : Answer : D

Description : At neutral pH Alanine has the following structure: (A) − − 2 3 H H N C COOH CH (B) + 3 − − 3 H H N C COO CH (C) 2 − − 3 H H N C COO CH (D) + 2 − − 3 H H N C COO CH

Last Answer : Answer : B

Description : A hexa peptide with 5 aspartic acid would have a net charge at pH 7: (A) Neutral (B) Positive (C) Negative (D) Not predictable

Last Answer : Answer : C

Description : When amino acids are treated with neutral formaldehyde, the pH of the mixture (A) Is not altered (B) Increases (C) Decreases (D) First increases then decreases

Last Answer : Answer : C

Description : At neutral pH, a mixture of amino acids in solution would be predominantly: (A) Dipolar ions (B) Nonpolar molecules (C) Positive and monovalent (D) Hydrophobic

Last Answer : Answer : A

Description : At neutral pH, a mixture of amino acids in solution would be predominantly: (A) Dipolar ions (B) Nonpolar molecules (C) Positive and monovalent (D) Hydrophobic

Last Answer : (A) Dipolar ions

Description : The advantage of following the IEEE Standard for drawing up a Project Management Plan (SPMP) - see IEEE Standard 1059.1 1987 - is a) It is drawn up by representatives from major  ... each organisation for a  particular applicationarea, development team or  technique. e) All of the above.

Last Answer : e) All of the above.

Description : Presence of arginine can be detected by (A) Sakaguchi reaction (B) Million-Nasse reaction (C) Hopkins-Cole reaction (D) Gas chromatography

Last Answer : Answer : A

Description : Frequently employed materials for the adsorption chromatography of proteins include (A) High capacity supporting gel (B) Starch blocks (C) Calcium phosphate gel alumina gel and hydroxy apatite (D) All of these

Last Answer : Answer : C

Description : The movement of charged particles towards one of the electrodes under the influence of electrical current is (A) Gel filtration (B) Molecular sieving (C) Gas liquid chromatography (D) Electrophoresis

Last Answer : Answer : D