Description : The technique for purification of proteins that can be made specific for a given protein is (A) Gel filtration chromotography (B) Ion exchange chromatography (C) Electrophoresis (D) Affinity chromatography
Last Answer : Answer : D
Description : What is the basic principle of ion-exchange chromatography?
Last Answer : Here, the separation is based on electrostatic attraction between charged molecules to oppositely charged groups on the ion exchange resins.
Description : This technique takes the advantage of the fact that each protein has different pH at which it is electrically neutral i.e., its isoelectric pH: (A) Isoelectric focussing (B) Immunoel Ectro Phoresis (C) Chromatography (D) HPLC
Last Answer : Answer : A
Description : The sorting out of molecules according to size and shape may be adapted to protein purification in this technique: (A) Adsorption chromatography (B) Gel filtration chromatography (C) Paper chromatography (D) None of these
Last Answer : Answer : B
Description : HPLC is an abbreviation for? A. High Profit Liquid Chromatography B. High Pressure Liquid Chromatography C. Higher Performance Low Chromatography D. Higher Profit Low Chromatography
Last Answer : High Pressure Liquid Chromatography
Description : Frequently employed materials for the adsorption chromatography of proteins include (A) High capacity supporting gel (B) Starch blocks (C) Calcium phosphate gel alumina gel and hydroxy apatite (D) All of these
Last Answer : Answer : C
Description : Primary structure of proteins can be determined by the use of (A) Electrophoresis (B) Chromatography (C) Ninhydrin (D) Sanger’s reagent
Description : An emf of the order of mV is generated when two solutions of different hydrogen ion concentration are separated by a thin glass wall". This is the working principle of a (A) pH meter (B) Polarimeter (C) Chromatograph (D) Polarograph
Last Answer : (A) pH meter
Description : The amino acid which gives yellow colour with Ninhydrin in paper chromatography is (A) Tyrosine (B) Proline (C) Tryptophan (D) Alanine
Description : What is the advantage of TLC over paper chromatography?
Last Answer : TLC needs lesser time, and separation is more effective.
Description : A sharp moving boundary is obtained between the pure solvent and solute containing layer in (A) Chromatography (B) Immuno Reactivity (C) Ultra Centrifugation (D) Solubility curve
Last Answer : Answer :C
Description : Human urine is usually acidic because (a) potassium and sodium exchange generates acidity (b) hydrogen ions are actively secreted into the filtrate (c) the sodium transporter exchanges one hydrogen ion for each sodium ion, in peritubular capillaries (d) excreted plasma proteins are acidic.
Last Answer : (b) hydrogen ions are actively secreted into the filtrate
Description : The movement of charged particles towards one of the electrodes under the influence of electrical current is (A) Gel filtration (B) Molecular sieving (C) Gas liquid chromatography (D) Electrophoresis
Description : A Zwitter ion is one which has in aqueous solution: (A) One positive charge and one negative charge (B) Two positive charges and one negative charge (C) Two negative charges and one positive charge (D) No electrical charges at all
Description : Radioactive elements can be traced out in living cell through a technique called – (1) Centrifugation (2) Chromatography (3) Auto radiography (4) All the above
Last Answer : (3) Auto radiography Explanation: Radioactive elements can be traced out in living cell through a technique called Autoradiography.
Description : The basis of the technique of chromatography for separating components of a mixture is? A. the differing movement of particles of different mass in an electrical field B. the interaction of the ... infrared radiation by the components. D. the deflection of charged particles in a magnetic field.
Last Answer : the interaction of the components with a stationary and a mobile phases
Description : Radioactive elements can be traced out in living cell through a technique called (1) Centrifugation (2) Chromatography (3) Auto radiography (4) All the above
Last Answer : Auto radiography
Description : The true statement about solutions of amino acids at physiological pH is (A) All amino acids contain both positive and negative charges (B) All amino acids contain positively charged side chains ... amino acids contain only positive charge (D) All amino acids contain negatively charged side chains
Description : The true statement about solutions of amino acids at physiological pH is (A) All amino acids contain both positive and negative charges (B) All amino acids contain positively charged side chains ... amino acids contain only positive Charge (D) All amino acids contain negatively charged side chains
Last Answer : (A) All amino acids contain both positive and negative charges
Description : One of the methods of purification of leach liquor is ion exchange, which involves (A) Exchange between two liquid phases (B) Exchange between a gaseous phase and a liquid phase (C) Exchange between a liquid phase and an organic resin phase (D) Exchange between a solid phase and a gas phase
Last Answer : (C) Exchange between a liquid phase and an organic resin phase
Description : Explain the principle of paper chromatography.
Last Answer : Paper chromatography is based on the difference in the rates at which the components of a mixture are adsorbed. The material on which different components are adsorbed is called ... compounds are adsorbed on stationary phase at different rates and separated by differential adsorption method.
Description : In a solution having a pH of 7.4, the hydrogen ion concentration is (A) 7.4 nmol/L (B) 40 nmol/L (C) 56 nmol/L (D) 80 nmol/L
Description : The oxygen d issoc iat ion curve for hemoglobin is shifted to the right by (A) Decreased O2 tension (B) Decreased CO2 tension (C) Increased CO2 tension (D) Increased pH
Description : PH is the negative logarithm of the hydrogen Ion Concentration express4ed in moles/liter of aqueous solution. PH of buffer solution is calculated by. Henderson's equation Name the best ... Chemical kinetics deals with study of. Rates and mechanisms of chemical and Biochemical reactions
Last Answer : What does Thermodynamics helps in predicting ?
Description : What is meant by gas-liquid chromatography ?
Last Answer : Gas-liquid chromatography is a type of partition chromatography , where the moving phase is an inert gas and the stationary phase is a liquid with a high boiling point absorbed on a solid surface. The ... . The components are dissolved due to the splitting of vapor , gas and liquid in the sample .
Description : In Gas-Liquid Chromatography, some of the samples need to be derivatized in order to increase their (A) volatility (B) solubility (C)thermal conductivity (D) polarizability
Last Answer : (D) polarizability
Description : Chloride shift is (A) H ions leaving the RBC in exchange of Cl- (B) Cl– leaving the RBC in exchange of bicarbonate (C) Bicarbonate ion returns to plasma and exchanged with chloride which shifts into the cell (D) Carbonic acid to the plasma
Description : From the information given below, calculate Debt service coverage Ratio- Net profit after interest and Tax Rs. 40,000, Depreciation Rs. 5,000, Rate of Income Tax 50%, 10% Mortgage Debentures Rs. 60,000. Fixed Interest Charges Rs. 6 ... . (A) 4 06 times (B) 5 06 times (C) 6 06 times (D) 7 06 times
Last Answer : Answer: 4•06 times
Description : When net protein utilization (NPU) is low, the requirements for proteins are (A) High (B) Moderate (C) Low (D) Supplementary
Description : pH is defined as the negative logarithm of the hydrogen ion concentration. Comparing a pH 5 and a pH 8 solution, which of the following is true? w) There are 1,000 times more hydrogen ions in ... . z) Cannot tell the difference between these two solutions by pH, since the numbers are so close.
Last Answer : ANSWER: X -- THERE ARE 1,000 TIMES MORE HYDROGEN IONS IN THE PH 5 SOLUTION
Description : The following are true about G proteins: a. they are first messengers b. when activated, the alpha subunit exchange GDP for GTP c. they are transmembrane signal receptor molecules d. vibrio cholerae secrets an exotoxin which makes G-proteins resistant to inactivation
Last Answer : when activated, the alpha subunit exchange GDP for GTP
Description : All the following statements about pepsin are correct except (A) It is smaller than pepsinogen (B) It is formed by the action of HCl on its precursor (C) Its optimum pH is 1.0–2.0 (D) It hydrolyses the C-terminal and N-terminal peptide bonds of proteins
Description : Presence of arginine can be detected by (A) Sakaguchi reaction (B) Million-Nasse reaction (C) Hopkins-Cole reaction (D) Gas chromatography
Description : Give an example of affinity chromatography.
Last Answer : Separation and quantitation of glycated hemoglobin.
Description : Give the principle of affinity chromatography.
Last Answer : The technique is based on the high affinity of specific proteins for specific chemical groups.
Description : What is the principle of gel filtration chromatography?
Last Answer : The separation is effected on the basis of the size of the molecules. It is otherwise called molecular sieving.
Description : What are the common types of partition chromatography?
Last Answer : Paper chromatography and thin layer chromatography.
Description : What is the principle of partition chromatography?
Last Answer : the components of the mixture to be separated are partitioned between the two phases depending on the partition co-efficient (solubility) of the particular substances.
Description : What is the principle of adsorption chromatography?
Last Answer : separation is based on differences in adsorption at the surface of a solid stationary medium.
Description : When the net charge on an amino acid is zero, the pH is maintained as? (A) 4.5 (B) 11.2 (C) 7.0 (D) 9.1
Description : Isoelectric pH of an amino acid is that pH at which it has a (A) Positive charge (B) Negative charge (C) Nil net charge (D) None of these
Description : The amino acids in which the R groups have a net positive charge at pH 7.0 are (A) Lysine, Arginine, Histidine (B) Lysine, Aspargine (C) Histidine, Aspargine (D) Glutamine, Arginine
Description : A hexa peptide with 5 aspartic acid would have a net charge at pH 7: (A) Neutral (B) Positive (C) Negative (D) Not predictable
Description : Isoelectric pH of an amino acid is that pH at which it has a (A) Positive charge (B) Negative charge (C) No net charge (D) All of these
Description : Which amino acid has a net positive charge at physiological pH?
Last Answer : Arginine and lysine.
Description : The isoelectric point of a protein is (a) the pH at which the protein molecule has no charges on its surface. (b) the pH at which a protein in solution has an equal number of positive and negative charges. (c) the electric charge under isothermal conditions. (b) None of these
Last Answer : the pH at which a protein in solution has an equal number of positive and negative charges
Description : “Rate of softening of water in Ion Exchange method is high,” Give reason.
Last Answer : Rate of softening of water in ion exchange method is high because, cation exchange beds and anions exchange beds are used this process, which remove all the hardness causing substances. It also removes alkali metals. This process is useful for acidic as well as alkaline water.
Description : The percentage of food nitrogen that is retained in the body represents (A) Digestibility coefficient (B) Biological value of proteins (C) Protein efficiency ratio (D) Net protein utilisation
Description : In a person increase in weight in gms per gm of protein consumption represents (A) Protein efficiency ratio (B) Digestibility value of proteins (C) Biological value of proteins (D) Net protein utilisation
Description : The percentage of nitrogen retained in the body after absorption of diet represents (A) Digestibility coefficient of proteins (B) Biological value of proteins (C) Protein efficiency ratio (D) Net protein utilisation