Description : The technique for purification of proteins that can be made specific for a given protein is (A) Gel filtration chromotography (B) Ion exchange chromatography (C) Electrophoresis (D) Affinity chromatography
Last Answer : Answer : D
Description : Given below are four statements pertaining to separation of DNA fragments using gel electrophoresis. Identify the incorrect statements. (i) DNA is negatively charged molecule and so it is loaded on gel towards the anode terminal. (ii) DNA ... (iii) (c) (ii), (iii) and (iv) (d) (i), (ii) and (iv)
Last Answer : (d) (i), (ii) and (iv)
Description : The basis of the technique of chromatography for separating components of a mixture is? A. the differing movement of particles of different mass in an electrical field B. the interaction of the ... infrared radiation by the components. D. the deflection of charged particles in a magnetic field.
Last Answer : the interaction of the components with a stationary and a mobile phases
Description : The sorting out of molecules according to size and shape may be adapted to protein purification in this technique: (A) Adsorption chromatography (B) Gel filtration chromatography (C) Paper chromatography (D) None of these
Last Answer : Answer : B
Description : What is the principle of gel filtration chromatography?
Last Answer : The separation is effected on the basis of the size of the molecules. It is otherwise called molecular sieving.
Description : What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis ? (a) The smaller the fragment size, the farther it moves. (b) Positively charged fragments move to farther end. ... charged fragments do not move. (d) The larger the fragment size, the farther it moves.
Last Answer : (a) The smaller the fragment size, the farther it moves
Description : What is the criterion for DN A fragments movement on agarose gel during gel electrophoresis ? (1) The smaller the fragment size, the farther it moves (2) Positively charged fragments move to farther ... Negatively charged fragments do not move (4) The larger the fragment size, the farther it moves
Last Answer : (1) The smaller the fragment size, the farther it moves
Description : Why does DNA move towards the anode in gel electrophoresis? -Biology
Last Answer : answer:
Description : Primary structure of proteins can be determined by the use of (A) Electrophoresis (B) Chromatography (C) Ninhydrin (D) Sanger’s reagent
Description : A high voltage applied to a gas discharge tube containing neon gas at very low pressure causes a current to flow through the tube. Which one of the following are the particles that flow towards the ... Are they w) electrons x) protons y) negatively charged neon ions z) positively charged neon ions
Last Answer : ANSWER: Z -- POSITIVELY CHARGED NEON IONS
Description : Frequently employed materials for the adsorption chromatography of proteins include (A) High capacity supporting gel (B) Starch blocks (C) Calcium phosphate gel alumina gel and hydroxy apatite (D) All of these
Last Answer : Answer : C
Description : Which of the following techniques is used to separate proteins based upon differences in their mass? (A) Isoelectric focusing (B) Dialysis (C) SDS-gel Electrophoresis (D) Western blotting
Description : A process in which substances are separated throug differences in the rates at which the components migrate is called: w) filtration x) chromatography y) elution z) titration
Last Answer : ANSWER: X -- CHROMATOGRAPHY
Description : The antibodies raised against a pure protein will show only one sharp spike on this technique: (A) Solubility curve (B) Solvent precipitation (C) Molecular weight determination (D) Immuno electrophoresis
Description : How long can I leave this 1% agarose gel out in room temperature before electrophoresis?
Last Answer : From surfing around, it looks like the gel will last several weeks at room temperature without drying out. I couldn’t find anything that indicated that which dye is used matters, but I doubt it.
Description : What is gel electrophoresis? -Biology
Description : What is agarose gel electrophoresis? -Biology
Description : What is agarose gel electrophoresis used for? -Biology
Description : What does agarose gel electrophoresis do? -Biology
Description : What is gel electrophoresis used for? -Biology
Description : How does gel electrophoresis separate DNA fragments?
Last Answer : There is a specific density of gel used in the electrophoresis.The DNA is placed in a well, and then electrical charge is used topull the DNA through the gel. Because spliced DNA is ... taxi cab, which is larger, movesslower through the traffic. The bicycle which is smaller, movesquicker.
Description : Why can gel electrophoresis separate dna fragments?
Last Answer : Electrophoresis enables you to distinguish DNA fragments of different lengths. DNA is negatively charged, therefore, when an electric current is applied to the gel, DNA will migrate towards the positively ... marker, you can work out the approximate length of the DNA fragments in the samples.
Description : Write the importance of gel electrophoresis during DNA finger print technology.
Last Answer : Gel electrophoresis helps to break down the DNA of an individual into short fragments from which a DNA finger print can be obtained.
Description : What is gel electrophoresis?
Last Answer : The technique of breaking down the DNA of individual into short segments using specific enzymes is called gel electrophoresis.
Description : Gel electrophoresis is used for (a) construction of recombinant DNA by joining with cloning vectors (b) isolation of DNA molecules (c) cutting of DNA into fragments (d) separation of DNA fragments according to their size.
Last Answer : (d) separation of DNA fragments according to their size.
Description : Agarose extracted from sea weeds is used in (a) spectrophotometry (b) tissue culture (c) PCR (d) gel electrophoresis
Last Answer : (d) gel electrophoresis.
Description : In gel electrophoresis, separated DNA fragments can be visualized with the help of (a) acetocarmine in bright blue light (b) ethidium bromide in UV radiation (c) acetocarmine in UV radiation (d) ethidium bromide in infrared radiation.
Last Answer : (b) ethidium bromide in UV radiation
Description : 6) In agarose gel electrophoresis, DNA molecules are separated on the basis of their- a)charge only b)size only c)charge to size ratio d)all of the above
Last Answer : d)all of the above
Description : 11. Gel electrophoresis is used for a) Construction of recombinant DNA by joining with cloning vectors b) Isolation of DNA molecules c) Cutting of DNA into fragments d)Separation of DNA fragments according to their size
Last Answer : d)Separation of DNA fragments according to their size
Description : Pick out the wrong statement. (A) The binary diffusivity in liquids is of the order of 10 -5 cm2 /sec (B) Molecular diffusion in solid is much faster than that in liquids ( ... Poiseuille's law, the permeability decreases with increase in temperature for flow of a gas through a given capillary
Last Answer : (B) Molecular diffusion in solid is much faster than that in liquids
Description : In electrical resistance welding, distortion results from the use of improper (A) Clamping methods (B) Electrodes (C) Current (D) Filler material
Last Answer : (A) Clamping methods
Description : The following technique makes use of the difference in net charges of proteins at a given pH: (A) Thin layer chromatography (B) Ion exchange chromatography (C) High performance liquid chromatography (D) Paper chromatography
Description : What is meant by gas-liquid chromatography ?
Last Answer : Gas-liquid chromatography is a type of partition chromatography , where the moving phase is an inert gas and the stationary phase is a liquid with a high boiling point absorbed on a solid surface. The ... . The components are dissolved due to the splitting of vapor , gas and liquid in the sample .
Description : In Gas-Liquid Chromatography, some of the samples need to be derivatized in order to increase their (A) volatility (B) solubility (C)thermal conductivity (D) polarizability
Last Answer : (D) polarizability
Description : The volume of nitrogen gas Um (measured at STP) required to cover a sample of silica get with a mono-molecular layer is `129 cm^(3) g^(-1)` of gel. Ca
Last Answer : The volume of nitrogen gas Um (measured at STP) required to cover a sample of silica get with a mono-molecular layer ... `16.2 xx 10^(-20) m^(2).`
Description : Rcmoval of carbon particles from air involves the principle of – (1) Precipitation (2) FiIteration (3) Electrophoresis (4) Sedimentation
Last Answer : (3) Electrophoresis Explanation: Carbon particles in air are colloidal in nature and carry negative charge. The removal of carbon parities from air involves the principle of electrophoresis. Carbon particles get ... losing their charge and, thus, the air which finally comes out is free from them.
Description : Removal of carbon particles from air involves the principle of - (1) Precipitation (2) Filteration (3) Electrophoresis (4) Sedimentation
Last Answer : (3) Electrophoresis Explanation: Electrophoresis is the process of separating certain large molecules so they can be examined more easily.
Description : Removal of carbon particles from air involves the principle of (1) Precipitation (2) Filteration (3) Electrophoresis (4) Sedimentation
Last Answer : Electrophoresis
Description : The property which forms the basis of sieving -Science
Last Answer : difference in size
Description : Wet sieving is employed, when the product contains __________ materials. (A) Abrasive (B) Large quantity of very fine (C) Coarse (D) Non-sticky
Last Answer : (B) Large quantity of very fine
Description : Explain the procedure to determine fineness of cement by dry sieving method. State its IS requirement.
Last Answer : Procedure to determine fineness of cement by dry sieving method: 1. Take the 100 gm of cement sample given using balance as initial weight as W1 gm. 2. Take 90 micron IS sieve and keep pan at bottom. 3. Place the ... . ii) Rapid hardening Cement (RHC): 5 % max. iii) Low Heat Cement (LHC): 5 % max.
Description : Presence of arginine can be detected by (A) Sakaguchi reaction (B) Million-Nasse reaction (C) Hopkins-Cole reaction (D) Gas chromatography
Last Answer : Answer : A
Description : Pick out the wrong statement. (A) Seam welding, projection welding & spot welding are the classification of electrical resistance welding (B) Electrode tip in spot welding is of copper and the ... joined are overlapped and placed between two electrodes (D) Mild steel sheet cannot be spot welded
Last Answer : (D) Mild steel sheet cannot be spot welded
Description : If an electric field is applied to a colloidal sol, the dispersed phase particles are found to move towards the electrode of opposite charge. If however, the dispersed phase is made stationary, the ... move in the opposite direction. What is the term used for such movement of dispersion medium ?
Last Answer : Ans. Electro osmosis.
Description : HPLC is an abbreviation for? A. High Profit Liquid Chromatography B. High Pressure Liquid Chromatography C. Higher Performance Low Chromatography D. Higher Profit Low Chromatography
Last Answer : High Pressure Liquid Chromatography
Description : you cannot be charged with dui for driving under the influence of legal prescription medication -General Knowledge
Last Answer : The given statement is false.
Description : Under the influence of electric field, the dielectric materials will get charged instantaneously. State True/False. a) True b) False
Last Answer : a) True
Description : Which of the following factors is responsible for the formation of concentrated urine? (a) Hydrostatic pressure during glomerular filtration. (b) Low levels of antidiuretic hormone. (c) ... the medullary interstitium in the kidneys. (d) Secretion of erythropoietin by Juxtaglomerular complex.
Last Answer : (c) Maintaining hyperosmolarity towards the medullary interstitium in the kidneys.