Description : The technique for purification of proteins that can be made specific for a given protein is (A) Gel filtration chromotography (B) Ion exchange chromatography (C) Electrophoresis (D) Affinity chromatography
Last Answer : Answer : D
Description : Frequently employed materials for the adsorption chromatography of proteins include (A) High capacity supporting gel (B) Starch blocks (C) Calcium phosphate gel alumina gel and hydroxy apatite (D) All of these
Last Answer : Answer : C
Description : The movement of charged particles towards one of the electrodes under the influence of electrical current is (A) Gel filtration (B) Molecular sieving (C) Gas liquid chromatography (D) Electrophoresis
Description : What is the principle of gel filtration chromatography?
Last Answer : The separation is effected on the basis of the size of the molecules. It is otherwise called molecular sieving.
Description : The following technique makes use of the difference in net charges of proteins at a given pH: (A) Thin layer chromatography (B) Ion exchange chromatography (C) High performance liquid chromatography (D) Paper chromatography
Last Answer : Answer : B
Description : This technique takes the advantage of the fact that each protein has different pH at which it is electrically neutral i.e., its isoelectric pH: (A) Isoelectric focussing (B) Immunoel Ectro Phoresis (C) Chromatography (D) HPLC
Last Answer : Answer : A
Description : What is the principle of adsorption chromatography?
Last Answer : separation is based on differences in adsorption at the surface of a solid stationary medium.
Description : Gel electrophoresis is used for (a) construction of recombinant DNA by joining with cloning vectors (b) isolation of DNA molecules (c) cutting of DNA into fragments (d) separation of DNA fragments according to their size.
Last Answer : (d) separation of DNA fragments according to their size.
Description : Two compounds I and II are eluted by column chromatography (adsorption of `I gt II`). Which one of the following is a correct statement ?
Last Answer : Two compounds I and II are eluted by column chromatography (adsorption of `I gt II`). Which one of the ... slower and has higher `R_(f)` value than I
Description : What is adsorption in chromatography?
Last Answer : Adsorption chromatography (AC) is chromatographic separation due to energetic interactions between the solute and the surface of the porous packings.
Description : Muddy water is treated with alum in purification process, it is termed as : (1) emulsification (2) absorption (3) adsorption (4) coagulation
Last Answer : (4) coagulation Explanation: Natural and wastewater contain small particulates that are suspended in water forming a colloid. These particles carry the same charges, and repulsion prevents them from combining into larger particulates to settle.
Description : Muddy water is treated with alum in purification process, itis termed as : (1) emulsification (2)absorption (3) adsorption (4) coagulation
Last Answer : coagulation
Description : A process in which substances are separated throug differences in the rates at which the components migrate is called: w) filtration x) chromatography y) elution z) titration
Last Answer : ANSWER: X -- CHROMATOGRAPHY
Description : The amino acid which gives yellow colour with Ninhydrin in paper chromatography is (A) Tyrosine (B) Proline (C) Tryptophan (D) Alanine
Description : What is the advantage of TLC over paper chromatography?
Last Answer : TLC needs lesser time, and separation is more effective.
Description : Which recursive sorting technique always makes recursive calls to sort subarrays that are about half size of the original array?
Last Answer : Mergesort always makes recursive calls to sort subarrays that are about half size of the original array, resulting in O(n log n) time.
Description : Coke oven gas is stripped of its H2S and mercaptans content by (A) Absorption in wash oil (B) Adsorption on bog iron bed (C) Bubbling it through dilute sulphuric acid (D) Adsorption on silica gel
Last Answer : (B) Adsorption on bog iron bed
Description : H2S present in gaseous stream can be removed by adsorption on (A) Silica gel (B) Active carbon (C) Bog iron (D) Limestone powder
Last Answer : (C) Bog iron
Description : Radioactive substances present in the polluted water stream can be removed by (A) Biological oxygen treatment (B) Coagulation and filtration (C) Adsorption in ion exchange materials (D) None of these
Last Answer : (C) Adsorption in ion exchange materials
Description : Which of the following assumptions of Freunlich adsorption isotherm is incorrect? (a) The constants are temperature dependent (b) It is valid over a range of pressure only (c) Adsorption ... equation is purely an empirical equation (d) The adsorbed molecules may be multimolecular in thickness
Last Answer : (d) The adsorbed molecules may be multimolecular in thickness
Description : Radioactive elements can be traced out in living cell through a technique called – (1) Centrifugation (2) Chromatography (3) Auto radiography (4) All the above
Last Answer : (3) Auto radiography Explanation: Radioactive elements can be traced out in living cell through a technique called Autoradiography.
Description : Radioactive elements can be traced out in living cell through a technique called (1) Centrifugation (2) Chromatography (3) Auto radiography (4) All the above
Last Answer : Auto radiography
Description : The basis of the technique of chromatography for separating components of a mixture is? A. the differing movement of particles of different mass in an electrical field B. the interaction of the ... infrared radiation by the components. D. the deflection of charged particles in a magnetic field.
Last Answer : the interaction of the components with a stationary and a mobile phases
Description : Assertion :- Filtration of colloidal particles is not possible by filter paper. Reason :- Size of colloidal particle is less than filter paper pores.
Last Answer : Assertion :- Filtration of colloidal particles is not possible by filter paper. Reason :- Size of ... D. If both Assertion & Reason are False.
Description : Explain the principle of paper chromatography.
Last Answer : Paper chromatography is based on the difference in the rates at which the components of a mixture are adsorbed. The material on which different components are adsorbed is called ... compounds are adsorbed on stationary phase at different rates and separated by differential adsorption method.
Description : __________ is the separation technique used for desalination of sea water. (A) Thermal diffusion (B) Reverse osmosis (C) Adsorption (D) Absorption
Last Answer : (B) Reverse osmosis
Description : Assertion(A): Physical adsorption of molecules on the surface requires activation energy. Reason(R): Because the bonds of adsorbed molecules are broke
Last Answer : Assertion(A): Physical adsorption of molecules on the surface requires activation energy. Reason(R): ... D. If both Assertion & Reason are False.
Description : What does protein expression and purification mean?
Last Answer : Protein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, usually cells, tissues or whole organisms. Protein purification is vital for ... the importance of building a convenient and easy to use protein high expression and purification system.
Description : What are the benefits of protein expression and purification?
Last Answer : Protein could be expressed in various systems and each system has its own advantages. To choose which system, we should take several factors into consideration such as the Cost, the type of ... protein yield. The most widely used systems are expressions in Bacterial, Yeast, Insect and Mammalian.
Description : Is protein expression and purification a scientific process?
Last Answer : Yes. Protein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, usually cells, tissues or whole organisms. Protein purification is vital ... vital for the characterization of the function, structure and interactions of the protein of interest.
Description : The process of separation and purification of expressed protein before marketing is called (a) downstream processing (b) bioprocessing (c) postproduction processing (d) upstream processing.
Last Answer : (a) downstream processing
Description : The process of separation and purification of expressed protein before marketing is called : (1) Downstream processing (2) Bioprocessing (3) Postproduction processing (4) Upstream processing
Last Answer : (1) Downstream processing
Description : Which of the following is the LEAST effective technique used to sterilize? a) heat b) cold c) ethylene oxide (pron: ETH-e-leen) d) filtration
Last Answer : ANSWER: B -- COLD
Description : Jigging is a technique by which different particles can be (A) Separated by particle size (B) Separated by particle density (C) Separated by particle shape (D) Mixed
Last Answer : (A) Separated by particle size
Description : When air passes through silica gel, A. it absorbs water vapour molecules B. latent heat of condensation is released C.dbt of air increases D. all of the mentioned
Last Answer : ANSWER : D
Description : 6) In agarose gel electrophoresis, DNA molecules are separated on the basis of their- a)charge only b)size only c)charge to size ratio d)all of the above
Last Answer : d)all of the above
Description : Before closing the work of the set for the day the work paper s must be examined by a) The Head Sorting Assistant b) The Senior most PA c) HRO d) None of these
Last Answer : a) The Head Sorting Assistant
Description : Whether the parcel bags and packet bags of the set are to be included in the transit bag or to be sent loose will be shown in a) The due mail list of the set b) The advance work paper of the set c) The sorting list of the set d) None of these
Last Answer : a) The due mail list of the set
Description : The primary force moving water molecules from the blood plasma to the interstitial fluid is a. active transport b. cotransport with H+ c. facilitated diffusion d. cotransport with Na+ e. filtration
Last Answer : filtration
Description : Assertion :- Zeolite is shape selective catalyst. Reason :- Size of the pores of the catalyst are such that they can trap only selective molecules.
Last Answer : Assertion :- Zeolite is shape selective catalyst. Reason :- Size of the pores of the catalyst are such ... . D. If both Assertion & Reason are False.
Description : Honey contain glucose and fructose along with some other ingredients, it has greater viscosity due to------? A. hydrogen bonding B. irregular shape of the molecules C. irregular shape of the molecules and strong inter-molecular forces (Answer) D. greater molecular size
Last Answer : C. irregular shape of the molecules and strong inter-molecular forces (Answer)
Description : Presence of arginine can be detected by (A) Sakaguchi reaction (B) Million-Nasse reaction (C) Hopkins-Cole reaction (D) Gas chromatography
Description : A sharp moving boundary is obtained between the pure solvent and solute containing layer in (A) Chromatography (B) Immuno Reactivity (C) Ultra Centrifugation (D) Solubility curve
Last Answer : Answer :C
Description : Primary structure of proteins can be determined by the use of (A) Electrophoresis (B) Chromatography (C) Ninhydrin (D) Sanger’s reagent
Description : Give an example of affinity chromatography.
Last Answer : Separation and quantitation of glycated hemoglobin.
Description : Give the principle of affinity chromatography.
Last Answer : The technique is based on the high affinity of specific proteins for specific chemical groups.
Description : What is the basic principle of ion-exchange chromatography?
Last Answer : Here, the separation is based on electrostatic attraction between charged molecules to oppositely charged groups on the ion exchange resins.
Description : What are the common types of partition chromatography?
Last Answer : Paper chromatography and thin layer chromatography.
Description : What is the principle of partition chromatography?
Last Answer : the components of the mixture to be separated are partitioned between the two phases depending on the partition co-efficient (solubility) of the particular substances.
Description : What is the shape of molecules SF6 and IF7 ?
Last Answer : SF6 is Octahedral IF7 is pentagonal Bipyramidal