Restriction endonucleases (A) Cut RNA chains at specific locations (B) Excise introns from hnRNA (C) Remove Okazaki fragments (D) Act as defensive enzymes to protect the host bacterial DNA from DNA of foreign organisms

Restriction endonucleases (A) Cut RNA chains at specific locations (B) Excise introns from hnRNA (C) Remove Okazaki fragments (D) Act as defensive enzymes to protect the host bacterial DNA from DNA of foreign organisms
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The normal function of restriction endonucleases is to (A) Excise introns from hrRNA (B) Polymerize nucleotides to form RNA (C) Remove primer from okazaki fragments (D) Protect bacteria from foreign DNA

Description : The normal function of restriction endonucleases is to (A) Excise introns from hrRNA (B) Polymerize nucleotides to form RNA (C) Remove primer from okazaki fragments (D) Protect bacteria from foreign DNA

Last Answer : Answer : D

Restriction endonucleases are enzymes which (a) make cuts at specific positions within the DNA molecule (b) recognize a specific nucleotide sequence for binding of DNA ligase (c) restrict the action of the enzyme DNA polymerase (d) remove nucleotides from the ends of the DNA molecule

Description : Restriction endonucleases are enzymes which (a) make cuts at specific positions within the DNA molecule (b) recognize a specific nucleotide sequence for binding of DNA ligase (c) restrict the action of the enzyme DNA polymerase (d) remove nucleotides from the ends of the DNA molecule

Last Answer : (a) make cuts at specific positions within the DNA molecule

Restriction endonucleases recognize and cut a certain sequence of (A) Single stranded DNA (B) Double stranded DNA (C) RNA (D) Protein

Description : Restriction endonucleases recognize and cut a certain sequence of (A) Single stranded DNA (B) Double stranded DNA (C) RNA (D) Protein

Last Answer : Answer : B

The restriction enzymes are used in genetic engineering, because (a) they can cut DNA at specific base sequence (b) they are nucleases that cut DNA at variable sites (c) they can degrade harmful proteins (d) they can join different DNA fragments.

Description : The restriction enzymes are used in genetic engineering, because (a) they can cut DNA at specific base sequence (b) they are nucleases that cut DNA at variable sites (c) they can degrade harmful proteins (d) they can join different DNA fragments.

Last Answer : (a) they can cut DNA at specific base sequence

The DNA fragments produced by the use of restriction endonucleases can be separated by

Description : 4. The DNA fragments produced by the use of restriction endonucleases can be separated by (a) polymerase chain reaction (b) gel electrophoresis (c) density gradient centrifugation (d) any of the above.

Last Answer : gel electrophoresis

Restriction endonucleases can recognise (A) Palindromic sequences (B) Chimeric DNA (C) DNA-RNA hybrids (D) Homopolymer sequences

Description : Restriction endonucleases can recognise (A) Palindromic sequences (B) Chimeric DNA (C) DNA-RNA hybrids (D) Homopolymer sequences

Last Answer : Answer : A

Restriction endonucleases split (A) RNA (B) Single stranded DNA (C) Double stranded DNA (D) DNA-RNA hybrids

Description : Restriction endonucleases split (A) RNA (B) Single stranded DNA (C) Double stranded DNA (D) DNA-RNA hybrids

Last Answer : Answer : C

Genetic engineering is possible, because (a) we can cut DNA at specific sites by endonucleases like DNase I (b) restriction endonucleases purified from bacteria can be used in vitro (c) the phenomenon of transduction in bacteria is well understood (d) we can see DNA by electron microscope

Description : Genetic engineering is possible, because (a) we can cut DNA at specific sites by endonucleases like DNase I (b) restriction endonucleases purified from bacteria can be used in vitro (c) the phenomenon of transduction in bacteria is well understood (d) we can see DNA by electron microscope

Last Answer : (b) restriction endonucleases purified from bacteria can be used in vitro

he molecular scissors use3d to cut DNA into specific genes of interest are called (a) Exonucleases (b) Restriction endonucleases (c) Ligases (d) Polymerases

Description : he molecular scissors use3d to cut DNA into specific genes of interest are called (a) Exonucleases (b) Restriction endonucleases (c) Ligases (d) Polymerases

Last Answer : (b) Restriction endonucleases

At a chromosome replication fork, the lagging strand consists of__ That is joined by___. a. RNA sequences; DNA ligase b. Okazaki Fragments; RNA Polymerase c. RNA sequences; ribosomes d. Okazaki fragments; DNA ligase

Description : At a chromosome replication fork, the lagging strand consists of__ That is joined by___. a. RNA sequences; DNA ligase b. Okazaki Fragments; RNA Polymerase c. RNA sequences; ribosomes d. Okazaki fragments; DNA ligase

Last Answer : d. Okazaki fragments; DNA ligase

All of the following statements about restriction endonucleases are true except: (A) They are present in bacteria (B) They act on double stranded DNA (C) They recognize palindromic sequences (D) They always produce sticky ends

Description : All of the following statements about restriction endonucleases are true except: (A) They are present in bacteria (B) They act on double stranded DNA (C) They recognize palindromic sequences (D) They always produce sticky ends

Last Answer : Answer : D

The cutting of DNA at specific locations became possible with the discovery of (a) selectable markers (b) ligases (c) restriction enzymes (d) probes.

Description : The cutting of DNA at specific locations became possible with the discovery of (a) selectable markers (b) ligases (c) restriction enzymes (d) probes.

Last Answer : (c) restriction enzymes

Which one of the following statements is true? a. Plasmids are circular molecules of RNA. b. RNA replicates by a conservative method of replication. c. Deletions or insertions of a nucleotide in a gene will cause a reading frameshift during translation. d. Okazaki fragments are removed from a RNA before translation occurs.

Description : Which one of the following statements is true? a. Plasmids are circular molecules of RNA. b. RNA replicates by a conservative method of replication. c. Deletions or insertions of a ... reading frameshift during translation. d. Okazaki fragments are removed from a RNA before translation occurs.

Last Answer : c. Deletions or insertions of a nucleotide in a gene will cause a reading frameshift during translation.

In sticky ends produced by restriction endonucleases (A) The 2 strands of DNA are joined to each other (B) The DNA strands stick to the restriction endonuclease (C) The ends of a double stranded fragment are overlapping (D) The ends of a double stranded fragment are non overlapping

Description : In sticky ends produced by restriction endonucleases (A) The 2 strands of DNA are joined to each other (B) The DNA strands stick to the restriction endonuclease (C) The ends of a double stranded fragment are overlapping (D) The ends of a double stranded fragment are non overlapping

Last Answer : Answer : C

DNA contains some palindromic sequences which (A) Mark the site for the formation of replication forks (B) Direct DNA polymerase to turn back to replicate the other strand (C) Are recognized by restriction enzymes (D) Are found only in bacterial DNA

Description : DNA contains some palindromic sequences which (A) Mark the site for the formation of replication forks (B) Direct DNA polymerase to turn back to replicate the other strand (C) Are recognized by restriction enzymes (D) Are found only in bacterial DNA

Last Answer : Answer : C

During DNA replication, Okazaki fragments are used to elongate (a) the lagging strand towards replication fork (b) the leading strand away from replication fork (c) the lagging strand away from the replication fork (d) the leading strand towards replication fork.

Description : During DNA replication, Okazaki fragments are used to elongate (a) the lagging strand towards replication fork (b) the leading strand away from replication fork (c) the lagging strand away from the replication fork (d) the leading strand towards replication fork.

Last Answer : (b) the leading strand away from replication fork

In the semiconservative method of replication a. Both parent strands are degraded. b. One parent strand is conserved in each of the the new dna. c. Both parent strands reform with one another. d. Okazaki fragments form both of the new molecules of DNA.

Description : In the semiconservative method of replication a. Both parent strands are degraded. b. One parent strand is conserved in each of the the new dna. c. Both parent strands reform with one another. d. Okazaki fragments form both of the new molecules of DNA.

Last Answer : b. One parent strand is conserved in each of the the new dna.

During DNA replication, Okazaki fragments are used to elongate: (1) The lagging strand towards replication fork. (2) The leading strand away from replication fork. (3) The lagging strand away from the replication fork. (4) The leading strand towards replication fork.

Description : During DNA replication, Okazaki fragments are used to elongate: (1) The lagging strand towards replication fork. (2) The leading strand away from replication fork. (3) The lagging strand away from the replication fork. (4) The leading strand towards replication fork.

Last Answer : (3) The lagging strand away from the replication fork.

Post-transcriptional modification of hnRNA involves all of the following except (A) Addition of 7-methylguanosine triphosphate cap (B) Addition of polyadenylate tail (C) Insertion of nucleotides (D) Deletion of introns

Description : Post-transcriptional modification of hnRNA involves all of the following except (A) Addition of 7-methylguanosine triphosphate cap (B) Addition of polyadenylate tail (C) Insertion of nucleotides (D) Deletion of introns

Last Answer : Answer : C

Okazaki pieces are made up of (A) RNA (B) DNA (C) RNA and DNA (D) RNA and proteins

Description : Okazaki pieces are made up of (A) RNA (B) DNA (C) RNA and DNA (D) RNA and proteins

Last Answer : Answer : C

Restriction endonucleases are (a) used for in vitro DNA synthesis (b) used in genetic engineering (c) synthesized by bacteria (d) present in mammalian cells for degradation of DNA.

Description : Restriction endonucleases are (a) used for in vitro DNA synthesis (b) used in genetic engineering (c) synthesized by bacteria (d) present in mammalian cells for degradation of DNA.

Last Answer : (b) used in genetic engineering

Restriction endonucleases (a) are present in mammalian cells for degradation of DNA when the cell dies (b) are used in genetic engineering for ligating two DNA molecules (c) are used for in vitro DNA synthesis (d) are synthesized by bacteria as part of their defense mechanism.

Description : Restriction endonucleases (a) are present in mammalian cells for degradation of DNA when the cell dies (b) are used in genetic engineering for ligating two DNA molecules (c) are used for in vitro DNA synthesis (d) are synthesized by bacteria as part of their defense mechanism.

Last Answer : (d) are synthesized by bacteria as part of their defense mechanism.

Without restriction endonucleases, it would be very difficult to a. Force Plasmids into Bacteria. b. Chemically Open Dna Molecules. c. Replicate Dna In A Recombinant Cell. d. Bring About Mutations In Bacteria.

Description : Without restriction endonucleases, it would be very difficult to a. Force Plasmids into Bacteria. b. Chemically Open Dna Molecules. c. Replicate Dna In A Recombinant Cell. d. Bring About Mutations In Bacteria.

Last Answer : b. Chemically Open Dna Molecules.

Read the following four statements (A-D) about certain mistakes in two of them. (A) The first transgenic buffalo, Rosie produced milk which was human alpha-lactalbumin enriched. (B) Restriction enzymes are used in isolation of DNA from other macromolecules. (C) Downstream processing is one of the steps of rDNA technology. (D) Disarmed pathogen vectors are also used in transfer of rDNA into the host. Which of the two statements have mistakes? (a) B and C (b) C and D (c) A and C (d) A and B

Description : Read the following four statements (A-D) about certain mistakes in two of them. (A) The first transgenic buffalo, Rosie produced milk which was human alpha-lactalbumin enriched. (B) Restriction enzymes are used in isolation of DNA ... mistakes? (a) B and C (b) C and D (c) A and C (d) A and B

Last Answer : (d) A and B

Restriction endonucleases are present in (A) Viruses (B) Bacteria (C) Eukaryotes (D) All of these

Description : Restriction endonucleases are present in (A) Viruses (B) Bacteria (C) Eukaryotes (D) All of these

Last Answer : Answer : B

During the process of isolation of DNA, chilled ethanol is added to (a) precipitate DNA (b) break open the cell to release DNA (c) facilitate action of restriction enzymes (d) remove proteins such as histones.

Description : During the process of isolation of DNA, chilled ethanol is added to (a) precipitate DNA (b) break open the cell to release DNA (c) facilitate action of restriction enzymes (d) remove proteins such as histones.

Last Answer : a) precipitate DNA

DNA fragments upto 45 kilobases in size can be cloned in (A) Bacterial plasmids (B) Lambda phage (C) Cosmids (D) Yeast artificial chromosomes

Description : DNA fragments upto 45 kilobases in size can be cloned in (A) Bacterial plasmids (B) Lambda phage (C) Cosmids (D) Yeast artificial chromosomes

Last Answer : Answer : C

Assetion: Restriction enzymes cut the strand 27 of DNA to produce sticky ends Reason : Stickiness of the ends faciliates the action of the enzyme DNA

Description : Assetion: Restriction enzymes cut the strand 27 of DNA to produce sticky ends Reason : Stickiness of the ends faciliates the action of the enzyme DNA

Last Answer : Assetion: Restriction enzymes cut the strand 27 of DNA to produce sticky ends Reason : Stickiness of ... D. If both assertion and reason are false

Identify the wrong statement with regard to restriction enzymes. (a) Each restriction enzyme functions by inspecting the length of a DNA sequence. (b) They cut the strand of DNA at palindromic sites. (c) They are useful in genetic engineering. (d) Sticky ends can be joined by using DNA ligases.

Description : Identify the wrong statement with regard to restriction enzymes. (a) Each restriction enzyme functions by inspecting the length of a DNA sequence. (b) They cut the strand of DNA at palindromic sites. (c) They are useful in genetic engineering. (d) Sticky ends can be joined by using DNA ligases.

Last Answer : d) Sticky ends can be joined by using DNA ligases.

Okasaki fragments are small bits of (A) RNA (B) DNA (C) DNA with RNA heads (D) RNA with DNA heads

Description : Okasaki fragments are small bits of (A) RNA (B) DNA (C) DNA with RNA heads (D) RNA with DNA heads

Last Answer : Answer : C

The complement system consists of a series of serum proteins that exist in a quiescent or very low-level state of activation in the uninfected host. Which of the following statement(s) is/are true concerning complement activation? a. The alternate (properdin) pathway of complement activation can occur directly through contact with fungal or bacterial cell wall compounds b. Complement component fragments may serve to decrease vascular permeability c. Excessive complement activation can produce deleterious effects d. Fragments of certain complement components serve as chemoattractants to additional cellular components of the host defense mechanism

Description : The complement system consists of a series of serum proteins that exist in a quiescent or very low-level state of activation in the uninfected host. Which of the following ... certain complement components serve as chemoattractants to additional cellular components of the host defense mechanism

Last Answer : Answer: a, c, d Complement activation can occur through either classic or alternate (properdin) pathways, both of which eventuate in deposition of terminal complement pathway components on the ... and release of lysosomal enzymes that can directly damage certain target tissues, such as the lung

Okazaki fragments give rise to

Description : Okazaki fragments give rise to

Last Answer : Okazaki fragments give rise to A. Master strand B. Sense strand C. Lagging strand D. Leading strand

DNA fragments generated by the restriction endo- nucleases in a chemical reaction can be separated by (a) electrophoresis (b) restriction mapping (c) centrifugation (d) polymerase chain reaction.

Description : DNA fragments generated by the restriction endo- nucleases in a chemical reaction can be separated by (a) electrophoresis (b) restriction mapping (c) centrifugation (d) polymerase chain reaction.

Last Answer : (a) electrophoresis

A foreign DNA and plasmid cut by the same restriction endonculease can be joined to form a recombinant plasmid using

Description : A foreign DNA and plasmid cut by the same restriction endonculease can be joined to form a recombinant plasmid using

Last Answer : A foreign DNA and plasmid cut by the same restriction endonculease can be joined to form a recombinant ... B. Ligase C. Eco RI D. Taq polymerase

A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using (a) EcoRI (b) Taq polymerase (c) polymerase III (d) ligase.

Description : A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using (a) EcoRI (b) Taq polymerase (c) polymerase III (d) ligase.

Last Answer : (d) ligase.

True statements about the nucleic acid include: a. contains purine and pyrimidine which are bound together by covalent bonds b. there is always an equal concentration of purine and pyrimidine c. in RNA, thymine is replaced by uracil d. introns is more common than exons on the DNA e. the histones mark the excision site

Description : True statements about the nucleic acid include: a. contains purine and pyrimidine which are bound together by covalent bonds b. there is always an equal concentration of purine and pyrimidine c. in RNA, ... d. introns is more common than exons on the DNA e. the histones mark the excision site

Last Answer : there is always an equal concentration of purine and pyrimidine

Which one of the following is common between prokaryotic and eukaryotic chromosomes? a. Presence or absence of introns. b. Loop or linear chromosomes. c. Genetic recombination occurrence in RNA. d. Mutations occur in the DNA.

Description : Which one of the following is common between prokaryotic and eukaryotic chromosomes? a. Presence or absence of introns. b. Loop or linear chromosomes. c. Genetic recombination occurrence in RNA. d. Mutations occur in the DNA.

Last Answer : d. Mutations occur in the DNA.

In the 18th century Carl Linnaeus started classifying organisms based a. Similarities in form b. DNA c. RNA d. Enzymes

Description : In the 18th century Carl Linnaeus started classifying organisms based a. Similarities in form b. DNA c. RNA d. Enzymes

Last Answer : a. Similarities in form

In the process of transcription in bacterial cells (A) Initiation requires rho protein (B) RNA polymerase incorporates methylated bases in correct sequence (C) Both the sigma unit and core enzymes of RNA polymerase are required for accurate promotor site binding (D) Primase is necessary for initiation

Description : In the process of transcription in bacterial cells (A) Initiation requires rho protein (B) RNA polymerase incorporates methylated bases in correct sequence (C) Both the sigma unit and core ... RNA polymerase are required for accurate promotor site binding (D) Primase is necessary for initiation

Last Answer : Answer : C

The enzyme DNA ligase (A) Introduces superhelical twists (B) Connects the end of two DNA chains (C) Unwinds the double helix (D) Synthesises RNA primers

Description : The enzyme DNA ligase (A) Introduces superhelical twists (B) Connects the end of two DNA chains (C) Unwinds the double helix (D) Synthesises RNA primers

Last Answer : Answer : B

Do eukaryotic cells have restriction endonucleases? -Biology

Description : Do eukaryotic cells have restriction endonucleases? -Biology

Last Answer : answer:

Which one of the following binds to specific nucleotide sequences that are upstream and most distant from the start site? (A) RNA polymerase (B) Repressor (C) Inducer (D) Restriction

Description : Which one of the following binds to specific nucleotide sequences that are upstream and most distant from the start site? (A) RNA polymerase (B) Repressor (C) Inducer (D) Restriction

Last Answer : Answer : A

Which one of the following binds to specific nucleotide sequences? (A) RNA polymerase (B) Repressor (C) Inducer (D) Restriction

Description : Which one of the following binds to specific nucleotide sequences? (A) RNA polymerase (B) Repressor (C) Inducer (D) Restriction

Last Answer : Answer : A

Mammalian RNA polymerase III synthesises (A) rRNA (B) mRNA (C) tRNA (D) hnRNA

Description : Mammalian RNA polymerase III synthesises (A) rRNA (B) mRNA (C) tRNA (D) hnRNA

Last Answer : Answer : C

Mammalian RNA polymerase I synthesises (A) mRNA (B) rRNA (C) tRNA (D) hnRNA

Description : Mammalian RNA polymerase I synthesises (A) mRNA (B) rRNA (C) tRNA (D) hnRNA

Last Answer : Answer : B

The smallest RNA among the following is (A) rRNA (B) hnRNA (C) mRNA (D) tRNA

Description : The smallest RNA among the following is (A) rRNA (B) hnRNA (C) mRNA (D) tRNA

Last Answer : Answer : D

Molecular weight of heterogenous nuclear RNA (hnRNA) is (A) More than 107 (B) 105 to 106 (C) 104 to 105 (D) Less than 104

Description : Molecular weight of heterogenous nuclear RNA (hnRNA) is (A) More than 107 (B) 105 to 106 (C) 104 to 105 (D) Less than 104

Last Answer : Answer : A

IgG cleaved by papain into (A) Two light and two heavy chains (B) Two Fab and one Fc fragments (C) Two pairs of one light and one heavy chain each (D) One Fab and two Fc fragments

Description : IgG cleaved by papain into (A) Two light and two heavy chains (B) Two Fab and one Fc fragments (C) Two pairs of one light and one heavy chain each (D) One Fab and two Fc fragments

Last Answer : Answer : B

Which of the following cut the DNA from specific places? (a) E.coli restriction endonuclease I (b) Ligase (c) Exonuclease (d) Alkaline phosphate

Description : Which of the following cut the DNA from specific places? (a) E.coli restriction endonuclease I (b) Ligase (c) Exonuclease (d) Alkaline phosphate

Last Answer : (a) E.coli restriction endonuclease I

Competence refers to a bacterial cell's ability to a. Take Up DNA Fragments From The Environment. b. Be Infected By A Transducing Phage. c. Produce A Protein Product From An Engineered Gene. d. Undergo Conjugation With An F+ Cell.

Description : Competence refers to a bacterial cell's ability to a. Take Up DNA Fragments From The Environment. b. Be Infected By A Transducing Phage. c. Produce A Protein Product From An Engineered Gene. d. Undergo Conjugation With An F+ Cell.

Last Answer : a. Take Up DNA Fragments From The Environment.