Description : Yeast artificial chromosome can be used to amplify DNA sequences of the size (A) Upto 10 kb (B) Upto 45 kb (C) Upto 100 kb (D) Upto 1,000 kb
Last Answer : Answer : D
Description : Lambda phage can be used to clone DNA fragments of the size (A) Upto 3 kilobases (B) Upto 20 kilobases (C) Upto 45 kilobases (D) Upto 1,000 kilobases
Last Answer : Answer : B
Description : DNA fragments upto 45 kilobases in size can be cloned in (A) Bacterial plasmids (B) Lambda phage (C) Cosmids (D) Yeast artificial chromosomes
Last Answer : Answer : C
Description : DNA contains some palindromic sequences which (A) Mark the site for the formation of replication forks (B) Direct DNA polymerase to turn back to replicate the other strand (C) Are recognized by restriction enzymes (D) Are found only in bacterial DNA
Description : Template-directed DNA synthesis occurs in all the following except (A) The replication fork (B) Polymerase chain reaction (C) Growth of RNA tumor viruses (D) Expression of oncogenes
Description : Template-directed DNA synthesis occurs in all the following except (A) The replication fork (B) Polymerase chain reaction (C) Growth of RNA tumor viruses (D) Expression of oneogenes
Description : In addition to Taq polymerase, polymerase chain reaction requires all of the following except (A) A template DNA (B) Deoxyribonucleoside triphosphates (C) Primers (D) Primase
Description : The DNA polymerase commonly used in polymerase chain reaction is obtained from (A) E. coli (B) Yeast (C) T.aquaticus (D) Eukaryotes
Description : At a chromosome replication fork, the lagging strand consists of__ That is joined by___. a. RNA sequences; DNA ligase b. Okazaki Fragments; RNA Polymerase c. RNA sequences; ribosomes d. Okazaki fragments; DNA ligase
Last Answer : d. Okazaki fragments; DNA ligase
Description : The role of molecular chaperones is to A-facilitate binding of ribosomes to mRNA B-degrade newly synthesized polypeptides that contain inaccurate sequences C-.facilitate binding of RNA polymerase to DNA D-aid a newly synthesized polypeptide in folding to its proper shape
Last Answer : -aid a newly synthesized polypeptide in folding to its proper shape
Last Answer : aid a newly synthesized polypeptide in folding to its proper shape
Description : Which one of the following binds to specific nucleotide sequences that are upstream and most distant from the start site? (A) RNA polymerase (B) Repressor (C) Inducer (D) Restriction
Last Answer : Answer : A
Description : Which one of the following binds to specific nucleotide sequences? (A) RNA polymerase (B) Repressor (C) Inducer (D) Restriction
Description : All of the following statements about eukaryotic promoters are true except (A) They may be located upstream or down stream from the structural gene (B) They have two consensus sequences ( ... (D) Mutations in promoter region can decrease the efficiency of transcription of the structural gene
Description : All of the following statements about bacterial promoters are true except (A) They are smaller than eukaryotic promoters (B) They have two consensus sequences upstream from the transcription star site (C) ... is the site for attachment of RNA polymerase (D) TATA box has a high melting temperature
Description : A scientist wants to make a dna fingerprint and she used polymerase chain reaction and restriction enzymes what should her next step be?
Last Answer : use restriction enzymes is the right answer for apex :)
Description : The following statement(s) is/are true concerning newer detection methods of systemic infection. a. Enzyme-linked immunosorbent assay (ELISA) is a rapid immunologic assay used for ... detected using advanced molecular techniques include cytomegalovirus (CMV) and human immunodeficiency virus (HIV)
Last Answer : Answer: a, b, c, d Although the classic detection of infection based on clinical signs of infection and bacterial culture remain the most common clinical tools, increasing ... including CMV and HIV. Furthermore, preliminary investigations into possible detection of fungal pathogens are underway
Description : The figure below shows three steps (A, B, C) of Polymerase Chain Reaction (PCR). Select the option giving correct identification together with what it represents? A. B. C ds DNA 3 5 5 3 ... - extension in the presence of heat stable DNA polymerase (d) A - annealing with two sets of primers
Last Answer : (c) C - extension in the presence of heat stable DNA polymerase
Description : DNA fragments generated by the restriction endo- nucleases in a chemical reaction can be separated by (a) electrophoresis (b) restriction mapping (c) centrifugation (d) polymerase chain reaction.
Last Answer : (a) electrophoresis
Description : Which of the following is not required for any of the techniques of DNA fingerprinting available at present? (a) Restriction enzymes (b) DNA-DNA hybridisation (c) Polymerase chain reaction (d) Zinc finger analysis
Last Answer : (d) Zinc finger analysis
Description : The correct statement concerning RNA and DNA polymerases is (A) RNA polymerase use nucleoside diphosphates (B) RNA polymerase require primers and add bases at 5' end of the growing polynucleotide ... and DNA polymerases can add nucleotides only at the 3' end of the growing polynucleotide chain
Description : Twenty cycles of PCR can amplify DNA: (A) 220 fold (B) 202 fold (C) 20 x 2 fold (D) 20 fold
Description : The enzyme used in polymerase chain reaction (PCR) is (A) Taq polymerase (B) RNA polymerase (C) Ribonuclease (D) Endonuclease
Description : The α-amino group of the new amino acyl tRNA in the A site carries out a nucleophilic attack on the esterified carboxyl group of the peptidyl tRNA occupying the P site. This reaction is catalysed by (A) DNA polymerase (B) RNA polymerase (C) Peptidyl transferase (D) DNA ligase
Description : In addition to DNA polymerase and primers what else does a polymerase chain require?
Last Answer : Need answer
Description : The genetic code operates via (A) The protein moiety of DNA (B) The base sequences of DNA (C) The nucleotide sequence of mRNA (D) The base sequence of tRNA
Description : DNA finger printing is based on the presence in DNA of (A) Constant number of tandem repeats (B) Varibale number of tandem repeats (C) Non-repititive sequences in each DNA (D) Introns in eukaryotic DNA
Description : All of the following statements about restriction endonucleases are true except: (A) They are present in bacteria (B) They act on double stranded DNA (C) They recognize palindromic sequences (D) They always produce sticky ends
Description : Restriction endonucleases can recognise (A) Palindromic sequences (B) Chimeric DNA (C) DNA-RNA hybrids (D) Homopolymer sequences
Description : Besides structural genes that encode proteins, DNA contains some regulatory sequences which are known as (A) Operons (B) Cistrons (C) Cis-acting elements (D) Trans-acting factors
Description : Satellite DNA contains (A) Highly repetitive sequences (B) Moderately repetitive sequences (C) Non-repetitive sequences (D) DNA-RNA hybrids
Description : What are the requirements for PCR (Polymerase Chain Reaction)? -Biology
Last Answer : answer:
Description : Steps involved in PCR (Polymerase Chain Reaction) process. -Biology
Last Answer : Step 1 DNA isolation.Step 2 Primer design.Step 3 Enzyme selection.Step 4 Thermal cycling.Step 5 Amplicon analysis.
Description : Why is polymerase chain reaction important? -Biology
Last Answer : Polymerase chain reaction (PCR) is often considered as one of the most important scientific advances in the field of molecular biology. With this revolutionary yet inexpensive biochemical technology, it's possible to generate millions of DNA copies from a single strand of DNA.
Description : What does polymerase chain reaction (pcr) do?
Last Answer : Polymerase chain reaction, or PCR, is a laboratory technique used to make multiple copies of a segment of DNA. PCR is very precise and can be used to amplify, or copy, a specific DNA target from a mixture of DNA molecules.
Description : Most commonly employed test for determining the antibody titers for Newcastle disease infection in B A. Agar gel precipitation test B. Hemagglutination inhibition C. Polymerase chain reaction D. None of these
Last Answer : Hemagglutination inhibition
Description : Most commonly employed test for determining the antibody titers for AI infection is B A. Agar gel precipitation test B. Hemagglutination inhibition C. Polymerase chain reaction D. None of these
Description : Trichomoniasis can be diagnosed by D A. Whiff test B. Polymerase chain reaction C. Wet mount D. All above
Last Answer : All above
Description : The correct order of steps in Polymerase Chain Reaction (PCR) is (a) extension, denaturation, annealing (b) annealing, extension, denaturation (c) denaturation, extension, annealing (d) denaturation, annealing, extension.
Last Answer : (d) denaturation, annealing, extension.
Description : The following are useful in the diagnosis of HIV infection: a. polymerase chain reaction b. antibodies by enzyme-liked immunoadsorbent assay c. P24 protein assay d. all above
Last Answer : all above
Description : Which of the following infection(s) can be diagnosed by the use of polymerase chain reaction? A- HIV-1 and HIV-2 viruses B- Hepatitis B virus C- Mycobacterium tuberculosis D- All of these
Last Answer : All of these
Description : An important method used in the rapid identification of a pathogen is a. RNA gene sequencing b. polymerase chain reaction c. molecular taxonomy d. biochemical tests
Last Answer : d. biochemical tests
Description : The correct order of steps in Polymerase Chain Reaction (PCR) is (1) Extension, Denaturation, Annealing (2) Annealing, Extension, Denaturation (3) Denaturation, Extension, Annealing (4) Denaturation, Annealing, Extension
Last Answer : (4) Denaturation, Annealing, Extension
Description : Which of the following molecule catalyzes the transpeptidation reaction? A.RNA polymerase B.Peptidyl transferase C.DNA ligase D.DNA polymerase
Last Answer : B.Peptidyl transferase
Description : Which of the following molecule catalyzes the transpeptidation reaction? A- RNA Polymerase B- Peptidlytransferase C- DNA ligase D- DNA polymerase
Last Answer : Peptidlytransferase
Description : Which of the following molecule catalyzes the transpeptidation reaction? A- RNA polymerase B- .Peptidyltransferase C- DNA ligase D- .DNA polymerase
Last Answer : .Peptidyltransferase