Description : Restriction endonucleases (A) Cut RNA chains at specific locations (B) Excise introns from hnRNA (C) Remove Okazaki fragments (D) Act as defensive enzymes to protect the host bacterial DNA from DNA of foreign organisms
Last Answer : Answer : D
Description : Restriction endonucleases are enzymes which (a) make cuts at specific positions within the DNA molecule (b) recognize a specific nucleotide sequence for binding of DNA ligase (c) restrict the action of the enzyme DNA polymerase (d) remove nucleotides from the ends of the DNA molecule
Last Answer : (a) make cuts at specific positions within the DNA molecule
Description : 4. The DNA fragments produced by the use of restriction endonucleases can be separated by (a) polymerase chain reaction (b) gel electrophoresis (c) density gradient centrifugation (d) any of the above.
Last Answer : gel electrophoresis
Description : Restriction endonucleases can recognise (A) Palindromic sequences (B) Chimeric DNA (C) DNA-RNA hybrids (D) Homopolymer sequences
Last Answer : Answer : A
Description : Restriction endonucleases split (A) RNA (B) Single stranded DNA (C) Double stranded DNA (D) DNA-RNA hybrids
Last Answer : Answer : C
Description : Restriction endonucleases recognize and cut a certain sequence of (A) Single stranded DNA (B) Double stranded DNA (C) RNA (D) Protein
Last Answer : Answer : B
Description : All of the following statements about restriction endonucleases are true except: (A) They are present in bacteria (B) They act on double stranded DNA (C) They recognize palindromic sequences (D) They always produce sticky ends
Description : At a chromosome replication fork, the lagging strand consists of__ That is joined by___. a. RNA sequences; DNA ligase b. Okazaki Fragments; RNA Polymerase c. RNA sequences; ribosomes d. Okazaki fragments; DNA ligase
Last Answer : d. Okazaki fragments; DNA ligase
Description : If no primer DNA was given, the following scientist could not have synthesized DNA. (A) Ochoa (B) Okazaki (C) Kornberg (D) Monod
Description : Restriction endonucleases are (a) used for in vitro DNA synthesis (b) used in genetic engineering (c) synthesized by bacteria (d) present in mammalian cells for degradation of DNA.
Last Answer : (b) used in genetic engineering
Description : Restriction endonucleases (a) are present in mammalian cells for degradation of DNA when the cell dies (b) are used in genetic engineering for ligating two DNA molecules (c) are used for in vitro DNA synthesis (d) are synthesized by bacteria as part of their defense mechanism.
Last Answer : (d) are synthesized by bacteria as part of their defense mechanism.
Description : Genetic engineering is possible, because (a) we can cut DNA at specific sites by endonucleases like DNase I (b) restriction endonucleases purified from bacteria can be used in vitro (c) the phenomenon of transduction in bacteria is well understood (d) we can see DNA by electron microscope
Last Answer : (b) restriction endonucleases purified from bacteria can be used in vitro
Description : Without restriction endonucleases, it would be very difficult to a. Force Plasmids into Bacteria. b. Chemically Open Dna Molecules. c. Replicate Dna In A Recombinant Cell. d. Bring About Mutations In Bacteria.
Last Answer : b. Chemically Open Dna Molecules.
Description : Restriction endonucleases are present in (A) Viruses (B) Bacteria (C) Eukaryotes (D) All of these
Description : Which one of the following statements is true? a. Plasmids are circular molecules of RNA. b. RNA replicates by a conservative method of replication. c. Deletions or insertions of a ... reading frameshift during translation. d. Okazaki fragments are removed from a RNA before translation occurs.
Last Answer : c. Deletions or insertions of a nucleotide in a gene will cause a reading frameshift during translation.
Description : In sticky ends produced by restriction endonucleases (A) The 2 strands of DNA are joined to each other (B) The DNA strands stick to the restriction endonuclease (C) The ends of a double stranded fragment are overlapping (D) The ends of a double stranded fragment are non overlapping
Description : In the semiconservative method of replication a. Both parent strands are degraded. b. One parent strand is conserved in each of the the new dna. c. Both parent strands reform with one another. d. Okazaki fragments form both of the new molecules of DNA.
Last Answer : b. One parent strand is conserved in each of the the new dna.
Description : During DNA replication, Okazaki fragments are used to elongate (a) the lagging strand towards replication fork (b) the leading strand away from replication fork (c) the lagging strand away from the replication fork (d) the leading strand towards replication fork.
Last Answer : (b) the leading strand away from replication fork
Description : During DNA replication, Okazaki fragments are used to elongate: (1) The lagging strand towards replication fork. (2) The leading strand away from replication fork. (3) The lagging strand away from the replication fork. (4) The leading strand towards replication fork.
Last Answer : (3) The lagging strand away from the replication fork.
Description : Okazaki pieces are made up of (A) RNA (B) DNA (C) RNA and DNA (D) RNA and proteins
Description : he molecular scissors use3d to cut DNA into specific genes of interest are called (a) Exonucleases (b) Restriction endonucleases (c) Ligases (d) Polymerases
Last Answer : (b) Restriction endonucleases
Description : An Okazaki fragment contains about (A) 10 Nucleotides (B) 100 Nucleotides (C) 1,000 Nucleotides (D) 10,000 Nucleotides
Description : In DNA sequencing, the primer A.specifies where the sequence ends B.specifies where the sequence begins C.both (a) and (b) D.generates variety of different sized fragments
Last Answer : B.specifies where the sequence begins
Description : The mechanism of synthesis of DNA and RNA are similar to all the following ways except (A) They involve release of pyrophosphate from each nucleotide added (B) They require activated nucleotide precursor and Mg2+ (C) The direction of synthesis is (D) They require a primer
Description : The mechanism of synthesis of DNA and RNA are similar in all the following ways except (A) They involve release of pyrophosphate from each nucleotide added (B) They require activated nucleotide precursor and Mg2+ (C) The direction of synthesis is 5’ → 3’ (D) They require a primer
Description : RNA synthesis requires (A) RNA primer (B) RNA template (C) DNA template (D) DNA primer
Description : Ribonucleotides of RNA primer are replaced by deoxyribonucleotides by the enzyme: (A) DNA polymerase I (B) DNA polymerase II (C) DNA polymerase III holoenzyme (D) All of these
Description : Deoxyribonucleotides are added to RNA primer by (A) DNA polymerase I (B) DNA polymerase II (C) DNA polymerase III holoenzyme (D) All of these
Description : RNA primer is formed by the enzyme: (A) Ribonuclease (B) Primase (C) DNA polymerase I (D) DNA polymerase III
Description : The common features of introns include all the following except (A) The base sequence begins with GU (B) The base sequence ends with AG (C) The terminal AG sequence is preceded by a purine rich tract of ten nucleotides (D) An adenosine residue in branch site participates in splicing
Description : Post-transcriptional modification of hnRNA involves all of the following except (A) Addition of 7-methylguanosine triphosphate cap (B) Addition of polyadenylate tail (C) Insertion of nucleotides (D) Deletion of introns
Description : Introns are present in DNA of (A) Viruses (B) Bacteria (C) Man (D) All of these
Description : If in a nucleic acid there are more than 8000 nucleotides it is most likely (A) RNA (B) DNA (C) Both (A) and (B) (D) None of these
Description : The correct statement concerning RNA and DNA polymerases is (A) RNA polymerase use nucleoside diphosphates (B) RNA polymerase require primers and add bases at 5' end of the growing polynucleotide ... and DNA polymerases can add nucleotides only at the 3' end of the growing polynucleotide chain
Description : Okasaki fragments are small bits of (A) RNA (B) DNA (C) DNA with RNA heads (D) RNA with DNA heads
Description : Okazaki fragments give rise to
Last Answer : Okazaki fragments give rise to A. Master strand B. Sense strand C. Lagging strand D. Leading strand
Description : The restriction enzymes are used in genetic engineering, because (a) they can cut DNA at specific base sequence (b) they are nucleases that cut DNA at variable sites (c) they can degrade harmful proteins (d) they can join different DNA fragments.
Last Answer : (a) they can cut DNA at specific base sequence
Description : DNA fragments generated by the restriction endo- nucleases in a chemical reaction can be separated by (a) electrophoresis (b) restriction mapping (c) centrifugation (d) polymerase chain reaction.
Last Answer : (a) electrophoresis
Description : True statements about the nucleic acid include: a. contains purine and pyrimidine which are bound together by covalent bonds b. there is always an equal concentration of purine and pyrimidine c. in RNA, ... d. introns is more common than exons on the DNA e. the histones mark the excision site
Last Answer : there is always an equal concentration of purine and pyrimidine
Description : Which one of the following is common between prokaryotic and eukaryotic chromosomes? a. Presence or absence of introns. b. Loop or linear chromosomes. c. Genetic recombination occurrence in RNA. d. Mutations occur in the DNA.
Last Answer : d. Mutations occur in the DNA.
Description : Do eukaryotic cells have restriction endonucleases? -Biology
Last Answer : answer:
Description : Would most likely happen if DNA polymerize were not functioning properly during DNA replication?
Last Answer : Need answer
Description : Similarity in DNA and RNA is that (a) both are polymer of nucleotides (b) both have similar pyrimidine (c) both have similar sugar (d) both are genetic material.
Last Answer : (b) both have similar pyrimidine
Last Answer : both are polymer of nucleotides
Description : Which of the following statement is correct? A- The size and sequence of introns can be deduced from the cDNA sequence B- Restriction endonuclease can cleave ss and dsDNA both C- Restriction ... sequenceof a gene D- Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide
Last Answer : Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide
Description : Which of the following statement is correct? A- The size and sequence of introns can be deduced from the cDNA sequence B- .Restriction endonuclease can cleave ss and dsDNA both C- Restriction ... of a Gene D- Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide
Description : After formation of replication fork (A) Both the new strands are synthesized discontinuously (B) One strand is synthesized continuously and the other discontinuously (C) Both the new strands are synthesized continuously (D) RNA primer is required only for the synthesis of one new strand
Description : Formation of RNA primer: (A) Precedes replication (B) Follows replication (C) Precedes transcription (D) Follows transcription
Description : The anticodon region is an important part of the structure of (A) rRNA (B) tRNA (C) mRNA (D) hrRNA