Description : The DNA polymerase commonly used in polymerase chain reaction is obtained from (A) E. coli (B) Yeast (C) T.aquaticus (D) Eukaryotes
Last Answer : Answer : C
Description : The optimum cardinal temparature point for germination of rice seeds is– 1) 20°C – 25°C 2) 18°C – 22°C 3) 30°C – 32°C 4) 37°C – 39°C
Last Answer : 1) 20°C – 25°C
Description : Match the organism with its use in biotechnology. (A) Bacillus (i) Cloning vector thuringiensis (B) Thermus (ii) Construction of first aquaticus rDNA molecule (C) Agrobacterium (iii)DNA polymerase tumefaciens (D) Salmonella (iv) Cry proteins ... (ii) (c) (iii) (ii) (iv) (i) (d) (iii) (iv) (i) (ii)
Last Answer : (b) (iv) (iii) (i) (ii)
Description : Potato tuber growth is stopped at what temperature ? (a) 40°C — 42°C (b) 30°C — 32°C (c) 35°C — 37°C (d) 38°C – 40°C
Last Answer : (b) 30°C — 32°C
Description : A microbe that is a microaerophilic mesophile would grow optimally at and a. high 02; 30°C b. low 02; 20°C c. no 02; 30°C d. low 02; 37°C
Last Answer : d. low 02; 37°C
Description : The Taq polymerase enzyme is obtained from (a) Bacillus subtilis (b) Pseudomonas putida (c) Thermus aquaticus (d) Thiobacillus ferroxidans.
Last Answer : (c) Thermus aquaticus
Description : Other things being suitable, what level of temperature is considered ideal for optimum growth of banana crop ? (A) 36°C (B) 18°C (C) 30°C (D) 12°C (E) 27°C
Last Answer : (C) 30°C
Description : The optimum temperature for the Banana crop is— (A) 30°C (B) 23°C (C) 21•5°C (D) 26•5°C
Last Answer : (B) 23°C
Description : Optimum temperature for potato tuber formation is a. 20-22°C b. 25-30°C c. 22-25°C d. All of these
Last Answer : 20-22°C
Description : Optimum temperature for cool season vegetables is: a. 25-30°C b. 25-27°C c. 18-24°C d. 15-20°C
Last Answer : 18-24°C
Description : Optimum temperature for maximum crop development in wheat is (a) 10-15°C(b) 20-25°C (c) 25-30°C (d) 30-35°C
Last Answer : (b) 20-25°C
Description : Which of the following are optimum temperature conditions for making benzenediazonium chloride from aniline? (a) 0°C to 10°C (b) 20°C to 25°C (c) 30°C to 40°C (d) 45°C to 50°C
Last Answer : 0°C to 10°C
Description : A perfect gas at 27°C is heated at constant pressure till its volume is double. The final temperature is (a) 54°C (b) 327°C (c) 108°C (d) 654°C (e) 600°C
Last Answer : Answer : b
Description : The normal temperature of the human body is – (1) 37°C (2) 37°F (3) 104°F (4) 36.8°C
Last Answer : (1) 37°C Explanation: The normal human body temperature is often stated as 36.5–37.5 °C.
Description : In vapour compression refrigeration system, if the evaporator temperature and the condenser temperatures are -13°C and 37°C respectively, the Carnot COP will be (A) 5.2 (B) 6.2 (C) 0.168 (D) Data insufficient, can't be found out
Last Answer : A) 5.2
Description : Template-directed DNA synthesis occurs in all the following except (A) The replication fork (B) Polymerase chain reaction (C) Growth of RNA tumor viruses (D) Expression of oncogenes
Description : The binding of prokaryotic DNA dependent RNA polymerase to promoter sits of genes is inhibited by the antibiotic: (A) Streptomycin (B) Rifamcin (C) Aueromycin (D) Puromycin
Last Answer : Answer : B
Description : Another name for reverse transcriptase is (A) DNA dependent DNA polymerase (B) DNA dependent RNA polymerase (C) RNA dependent DNA polymerase (D) RNA dependent RNA polymerase
Description : Template-directed DNA synthesis occurs in all the following except (A) The replication fork (B) Polymerase chain reaction (C) Growth of RNA tumor viruses (D) Expression of oneogenes
Description : RNA directed DNA polymerase is (A) Replicase (B) Transcriptase (C) Reversetranscriptase (D) Polymerase–III
Description : DNA directed RNA polymerase is (A) Replicase (B) Transcriptase (C) Reverse transcriptase (D) Polymerase III
Description : The antibiotic which inhibits DNA dependent RNA polymerase is (A) Mitomycin C (B) Actinomycin d (C) Streptomycin (D) Puromycin
Description : In addition to Taq polymerase, polymerase chain reaction requires all of the following except (A) A template DNA (B) Deoxyribonucleoside triphosphates (C) Primers (D) Primase
Last Answer : Answer : D
Description : Polymerase chain reaction can rapidly amplify DNA sequences of the size (A) Upto 10 kilobases (B) Upto 45 kilobases (C) Upto 100 kilobases(D) Upto 1,000 kilobases
Last Answer : Answer : A
Description : Myeloma cells and lymphocytes can be fused by using (A) Calcium chloride (B) Ethidium bromide (C) Polyethylene glycol (D) DNA polymerase
Description : Xeroderma pigmentosum results from a defect in (A) uvr ABC excinuclease (B) DNA polymerase I (C) DNA ligase (D) All of these
Description : Damage to DNA caused by ultraviolet radiation can be repaired by (A) uvr ABC excinuclease (B) DNA polymerase I (C) DNA ligase (D) All of these
Description : α-Amanitin inhibits (A) DNA polymerase II of prokaryotes (B) DNA polymerase α of eukaryotes (C) RNA polymerase II of eukaryotes (D) RNA-dependent DNA polymerase
Description : DNA contains some palindromic sequences which (A) Mark the site for the formation of replication forks (B) Direct DNA polymerase to turn back to replicate the other strand (C) Are recognized by restriction enzymes (D) Are found only in bacterial DNA
Description : Ciprofloxacin inhibits (A) DNA topisomerase II (B) DNA polymerase I (C) DNA polymerase III (D) DNA gyrase
Description : The mammalian DNA polymerase involved in error correction is (A) DNA polymerase α (B) DNA polymerase β (C) DNA polymerase γ (D) DNA polymerase δ
Description : Primase activity is present in (A) DNA polymerase II (B) DNA polymerase α (C) DNA polymerase β (D) DNA polymerase δ
Description : Replication of nuclear DNA in mammals is catalysed by (A) DNA polymerase α (B) DNA polymerase β (C) DNA polymerase γ (D) DNA polymerase III
Description : Mammalian DNA polymerase γ is located in (A) Nucleus (B) Nucleolus (C) Mitochondria (D) Cytosol
Description : A mammalian DNA polymerase among the following is (A) DNA polymerase α (B) DNA polymerase I (C) DNA polymerase II (D) DNA polymerase IV
Description : A consensus sequence on DNA, called TATA box, is the site for attachment of (A) RNA-dependent DNA polymerase (B) DNA-dependent RNA polymerase (C) DNA-dependent DNA polymerase (D) DNA topoisomerase II
Description : The termination site for transcription is recognized by (A) α−Subunit of DNA-dependent RNA polymerase (B) β−Subunit of DNA-dependent RNA polymerase (C) Sigma factor (D) Rho factor
Description : The initiation site for transcription is recognized by (A) α−Subunit of DNA-dependent RNA polymerase (B) β−Subunit of DNA-dependent RNA polymerase (C) Sigma factor (D) Rho factor
Description : DNA-dependent RNA polymerase requires the following for its catalytic activity: (A) Mg++ (B) Mn++ (C) Both (A) and (B) (D) None of these
Description : DNA-dependent RNA polymerase is a (A) Monomer (B) Dimer (C) Trimer (D) Tetramer
Description : All of the following statements about RNA-dependent DNA polymerase are true except: (A) It synthesizes DNA using RNA as a template (B) It is also known as reverse transcriptase (C) It synthesizes DNA in 5’→3’ direction (D) It is present in all the viruses
Description : 3’→5’ Exonuclease activity of DNA polymerase I (A) Removes ribonucleotides (B) Adds deoxyribonucleotides (C) Corrects errors in replication (D) Hydrolyses DNA into mononucleotides
Description : DNA polymerase I possesses (A) Polymerase activity (B) 3’→5’ Exonuclease activity (C) 5’→3’ Exonuclease activity (D) All of these
Description : DNA polymerase III holoenzyme possesses (A) Polymerase activity (B) 3’→5’ Exonuclease activity (C) 5’→3’ Exonuclease and polymerase activities (D) 3’→5’ Exonuclease and polymerase activities
Description : Reverse transcriptase activity is present in the eukaryotic: (A) DNA polymerase α (B) DNA polymerase γ (C) Telomerase (D) DNA polymerase II
Description : Negative supercoils are introduced in DNA by (A) Helicase (B) DNA ligase (C) DNA gyrase (D) DNA polymerase III holoenzyme
Description : DNA fragments are sealed by (A) DNA polymerase II (B) DNA ligase (C) DNA gyrase (D) DNA topoisomerase II
Description : Ribonucleotides of RNA primer are replaced by deoxyribonucleotides by the enzyme: (A) DNA polymerase I (B) DNA polymerase II (C) DNA polymerase III holoenzyme (D) All of these
Description : Deoxyribonucleotides are added to RNA primer by (A) DNA polymerase I (B) DNA polymerase II (C) DNA polymerase III holoenzyme (D) All of these
Description : RNA primer is formed by the enzyme: (A) Ribonuclease (B) Primase (C) DNA polymerase I (D) DNA polymerase III