Description : The technique for purification of proteins that can be made specific for a given protein is (A) Gel filtration chromotography (B) Ion exchange chromatography (C) Electrophoresis (D) Affinity chromatography
Last Answer : Answer : D
Description : In DNA finger printing method, the transfer of DNA from gel to nylon membrane is called________ a. Western Blotting b. Eastern Blotting c. Southern Blotting d. Northern Blotting
Last Answer : c. Southern Blotting
Description : How does gel electrophoresis separate DNA fragments?
Last Answer : There is a specific density of gel used in the electrophoresis.The DNA is placed in a well, and then electrical charge is used topull the DNA through the gel. Because spliced DNA is ... taxi cab, which is larger, movesslower through the traffic. The bicycle which is smaller, movesquicker.
Description : Why can gel electrophoresis separate dna fragments?
Last Answer : Electrophoresis enables you to distinguish DNA fragments of different lengths. DNA is negatively charged, therefore, when an electric current is applied to the gel, DNA will migrate towards the positively ... marker, you can work out the approximate length of the DNA fragments in the samples.
Description : In vitro clonal propagation in plants is characterized by (a) PCR and RAPD (b) Northern blotting (c) electrophoresis and HPLC (d) microscopy.
Last Answer : (a) PCR and RAPD
Description : An analysis of chromosomal DNA using the Southern hybridization technique does not use (a) electrophoresis (b) blotting (c) autoradiography (d) PCR.
Last Answer : (d) PCR.
Description : The movement of charged particles towards one of the electrodes under the influence of electrical current is (A) Gel filtration (B) Molecular sieving (C) Gas liquid chromatography (D) Electrophoresis
Description : A particular protein in a mixture can be detected by A) Southern blotting (B) Northern blotting (C) Western blotting (D) None of these
Last Answer : Answer : C
Description : An antibody probe is used in (A) Southern blotting (B) Northern blotting (C) Western blotting (D) None of these
Description : A particular RNA in a mixture can be identified by (A) Western blotting (B) Eastern blotting (C) Northern blotting (D) Southern blotting
Description : Fragments of DNA can be identified by the technique of (A) Western blotting (B) Eastern blotting (C) Northern blotting (D) Southern blotting
Description : The solubility of most proteins is lowered at high salt concentrations is called as (A) Salting in process (B) Salting out process (C) Isoelectric focussing(D) None of these
Last Answer : Answer : B
Description : In the process of dialysis, used on patients with affected kidneys, the phenomenon involved is (a) diffusion (b) absorption (c) osmosis (d) electrophoresis
Last Answer : Ans:(c)
Description : In the process of dialysis, used on patients with affected kidneys, the phenomenon involved is (1) Diffusion (2) Absorption (3) Osmosis (4) Electrophoresis
Last Answer : (3) Osmosis Explanation: Dialysis is the artificial process of getting rid of waste (diffusion) and unwanted water (ultrafiltration) from the blood. This process is naturally done by our kidneys. It ... it results in osmotic pressure which causes the fluid to move from the blood into the dialysate.
Description : Explain the following terms : (i) Electrophoresis, (ii) Coagulation, (iii) Dialysis (iV) Tyndal Effect
Last Answer : (i) Electrophoresis :- The immigration of colloidal solute towards oppositely charged electrode under an electric potential is called Electrophoresis. (ii) Coagulation :- The process of settling down ... The scattering of light in colloidal solution by colloidal solute is known as Tyndal effect.
Description : In the process of dialysis, used on patients with affected kidneys, the phenomenon involved is (1) Diffusion(2) Absorption (3) Osmosis (4) Electrophoresis
Last Answer : Osmosis
Description : Gel electrophoresis is used for (a) construction of recombinant DNA by joining with cloning vectors (b) isolation of DNA molecules (c) cutting of DNA into fragments (d) separation of DNA fragments according to their size.
Last Answer : (d) separation of DNA fragments according to their size.
Description : 6) In agarose gel electrophoresis, DNA molecules are separated on the basis of their- a)charge only b)size only c)charge to size ratio d)all of the above
Last Answer : d)all of the above
Description : Hormone receptors that stimulate cAMP production (A) are part of a complex of two proteins that transform the external signal into internal cAMP production (B) are proteins distinct and separate from those ... binding of the hormone (D) are not very specific and bind a number of different hormones
Description : How long can I leave this 1% agarose gel out in room temperature before electrophoresis?
Last Answer : From surfing around, it looks like the gel will last several weeks at room temperature without drying out. I couldn’t find anything that indicated that which dye is used matters, but I doubt it.
Description : Why does DNA move towards the anode in gel electrophoresis? -Biology
Last Answer : answer:
Description : What is gel electrophoresis? -Biology
Description : What is agarose gel electrophoresis? -Biology
Description : What is agarose gel electrophoresis used for? -Biology
Description : What does agarose gel electrophoresis do? -Biology
Description : What is gel electrophoresis used for? -Biology
Description : Write the importance of gel electrophoresis during DNA finger print technology.
Last Answer : Gel electrophoresis helps to break down the DNA of an individual into short fragments from which a DNA finger print can be obtained.
Description : What is gel electrophoresis?
Last Answer : The technique of breaking down the DNA of individual into short segments using specific enzymes is called gel electrophoresis.
Description : Agarose extracted from sea weeds is used in (a) spectrophotometry (b) tissue culture (c) PCR (d) gel electrophoresis
Last Answer : (d) gel electrophoresis.
Description : What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis ? (a) The smaller the fragment size, the farther it moves. (b) Positively charged fragments move to farther end. ... charged fragments do not move. (d) The larger the fragment size, the farther it moves.
Last Answer : (a) The smaller the fragment size, the farther it moves
Description : Given below are four statements pertaining to separation of DNA fragments using gel electrophoresis. Identify the incorrect statements. (i) DNA is negatively charged molecule and so it is loaded on gel towards the anode terminal. (ii) DNA ... (iii) (c) (ii), (iii) and (iv) (d) (i), (ii) and (iv)
Last Answer : (d) (i), (ii) and (iv)
Description : In gel electrophoresis, separated DNA fragments can be visualized with the help of (a) acetocarmine in bright blue light (b) ethidium bromide in UV radiation (c) acetocarmine in UV radiation (d) ethidium bromide in infrared radiation.
Last Answer : (b) ethidium bromide in UV radiation
Description : 11. Gel electrophoresis is used for a) Construction of recombinant DNA by joining with cloning vectors b) Isolation of DNA molecules c) Cutting of DNA into fragments d)Separation of DNA fragments according to their size
Last Answer : d)Separation of DNA fragments according to their size
Description : What is the criterion for DN A fragments movement on agarose gel during gel electrophoresis ? (1) The smaller the fragment size, the farther it moves (2) Positively charged fragments move to farther ... Negatively charged fragments do not move (4) The larger the fragment size, the farther it moves
Last Answer : (1) The smaller the fragment size, the farther it moves
Description : Plasma proteins are isolated by (A) Salting out (B) Electrophoresis (C) Flourimetry (D) Both (A) and (B)
Description : Primary structure of proteins can be determined by the use of (A) Electrophoresis (B) Chromatography (C) Ninhydrin (D) Sanger’s reagent
Description : What is western blotting ?
Last Answer : Identification of specific protein in a complex mixture of proteins can be accomplished bye a technique that is known as western blotting.
Description : The blotting technique used to identify the isolated protein is - (1) Northern blotting (2) Western blotting (3) Southern blotting (4) Cloning
Last Answer : (2) Western blotting Explanation: Protein electrophoresis and Western blotting are both methods used to identify specific proteins in a sample or solution. Western blotting, also known as immunoblotting or ... to detect the presence of a specific protein in a complex mixture extracted from cells.
Description : With regard to DNA molecules: a. they contain adenine, cytosine, guanine and uracil bases b. they can be detected with Western blotting c. they can be detected with Southern blotting d. they are denatured at temperature of 1000C
Last Answer : they can be detected with Southern blotting
Description : The blotting technique used to identify the isolated protein is (1) Northern blotting (2) Western blotting (3) Southern blotting (4) Cloning
Last Answer : Western blotting
Description : The following statement(s) is/are true concerning newer detection methods of systemic infection. a. Enzyme-linked immunosorbent assay (ELISA) is a rapid immunologic assay used for ... detected using advanced molecular techniques include cytomegalovirus (CMV) and human immunodeficiency virus (HIV)
Last Answer : Answer: a, b, c, d Although the classic detection of infection based on clinical signs of infection and bacterial culture remain the most common clinical tools, increasing ... including CMV and HIV. Furthermore, preliminary investigations into possible detection of fungal pathogens are underway
Description : Frequently employed materials for the adsorption chromatography of proteins include (A) High capacity supporting gel (B) Starch blocks (C) Calcium phosphate gel alumina gel and hydroxy apatite (D) All of these
Description : Dialysis can separate which in addition to the glucose from human blood?
Last Answer : Protein
Description : 1. Whow resigned as Vice-President to contest for the office of the President? 2. Dialysis can separate which in addition to the glucose from human blood? 3. Who was the first Indian cricketer to ... do the Eastern Ghats and the Western Ghats meet? 20. Who stabbed Guru Govind Singh to death ?
Last Answer : Answer : 1. V.V. Giri 2. Protein 3. Mohinder Arnarnath 4. Coromandal Coast 5. Bal Gangadhar Tilak 6. 25 minutes 7. Oviduct 8. President 9. Venus 10. Jaunpur 11. Dispersion 12. Clearing ... December, 22 15. Vardhaman Mahavira 16. Thigh bone 17. Permanency18. Methane 19. Nilgiri Hills 20. Gul Khan
Description : In the genetic disorder of cystinuria, the patient excretes large quantities of cystine in their urine and its low solubility causes crystalline cystine to precipitate as stones in kidneys. The remedy ... C) NaHCO3 prevents stone formation by hydrolysis of cystine to cysteine (D) None of these
Description : If two persons with AB' blood group marry and have sufficiently large number of children, these children could be classified as A' blood group: AB' blood group : B' blood group in 1 ... in an example of (a) partial dominance (b) complete dominance (c) codominance (d) incomplete dominance.
Last Answer : (c) codominance
Description : Irreversible precipitation of proteins caused by heating is called : (a) Polymerisation (b) Denaturation (c) Electrophoresis (d) Inversion
Last Answer : Denaturation
Description : The Michaehis-Menten hypothesis: (A) Postulates the formation of an enzyme substrate complex (B) Enables us to calculate the isoelectric point of an enzyme (C) States that the ... independent of substrate concentration (D) States that the reaction rate is proportional to substrate concentration
Last Answer : Answer : A