Description : Sanger’s reagent contains (A) Phenylisothiocyanate (B) Dansyl chloride (C) 1-Fluoro-2, 4-dinitrobenzene (D) Ninhydrin
Last Answer : Answer : C
Description : The technique for purification of proteins that can be made specific for a given protein is (A) Gel filtration chromotography (B) Ion exchange chromatography (C) Electrophoresis (D) Affinity chromatography
Last Answer : Answer : D
Description : N-terminal amino acids of a polypeptide are estimated by (A) Edmann reaction (B) Sanger’s reagent (C) Formaldehyde test (D) Ninhydrine reaction
Last Answer : Answer : A
Description : The amino acid which gives yellow colour with Ninhydrin in paper chromatography is (A) Tyrosine (B) Proline (C) Tryptophan (D) Alanine
Last Answer : Answer : B
Description : The useful reagent for detection of amino acids is (A) Molisch reagent (B) Dichlorophenol Indophenol (C) Ninhydrin (D) Biuret
Description : Base sequence of DNA can be determined by (A) Maxam-Gilbert method (B) Sanger’s dideoxy method (C) Both (A) and (B) (D) None of these
Description : The movement of charged particles towards one of the electrodes under the influence of electrical current is (A) Gel filtration (B) Molecular sieving (C) Gas liquid chromatography (D) Electrophoresis
Description : What are the Reaction of amino acid using ninhydrin and biuret reagent?
Last Answer : What is the answer ?
Description : Do proteins give a color with ninhydrin?
Last Answer : Proteins do not give a true color reaction; but N- terminal end amino group of protein will react with ninhydrin, to produce a blue color.
Description : The following technique makes use of the difference in net charges of proteins at a given pH: (A) Thin layer chromatography (B) Ion exchange chromatography (C) High performance liquid chromatography (D) Paper chromatography
Description : Frequently employed materials for the adsorption chromatography of proteins include (A) High capacity supporting gel (B) Starch blocks (C) Calcium phosphate gel alumina gel and hydroxy apatite (D) All of these
Description : Which of the following techniques is used to separate proteins based upon differences in their mass? (A) Isoelectric focusing (B) Dialysis (C) SDS-gel Electrophoresis (D) Western blotting
Description : Plasma proteins are isolated by (A) Salting out (B) Electrophoresis (C) Flourimetry (D) Both (A) and (B)
Description : Which of the following tests is not used for testing proteins? (a) Ninhydrin test (b) Biuret test (c) Xanthoproteic test (d) Tollens' test
Last Answer : Tollens' test
Description : Ninhydrin test is given by (a) Carbohydrates (b) Proteins (c) Alkanes (d) Alkenes
Last Answer : Proteins
Description : In sanger’s method of DNA sequence determination, DNA synthesis is stopped by using (A) 1′, 2′- Dideoxyribonucleoside triphosphates (B) 2′, 3′- Dideoxyribonucleoside triphosphates (C) 2′, 4′- Dideoxyribonucleoside triphosphates (D) 2′, 5′ - Dideoxyribonucleoside triphosphates
Description : Proteins react with biuret reagent which is suggestive of 2 or more (A) Hydrogen bonds (B) Peptide bonds (C) Disulphide bonds (D) Hydrophobic bonds
Description : Isoenzymes can be characterized by (A) Proteins lacking enzymatic activity that are necessary for the activation of enzymes (B) Proteolytic enzymes activated by hydrolysis (C) Enzymes with identical primary structure (D) Similar enzymes that catalyse different reaction
Description : During denaturation of proteins, all of the following are disrupted except (A) Primary structure (B) Secondary structure (C) Tertiary structure (D) Quaternary structure
Description : Denaturation of proteins results in (A) Disruption of primary structure (B) Breakdown of peptide bonds (C) Destruction of hydrogen bonds (D) Irreversible changes in the molecule
Description : In proteins the α-helix and β-pleated sheet are examples of (A) Primary structure (B) Secondary structure (C) Tertiary structure (D) Quaternary structure
Description : If two persons with AB' blood group marry and have sufficiently large number of children, these children could be classified as A' blood group: AB' blood group : B' blood group in 1 ... in an example of (a) partial dominance (b) complete dominance (c) codominance (d) incomplete dominance.
Last Answer : (c) codominance
Description : Irreversible precipitation of proteins caused by heating is called : (a) Polymerisation (b) Denaturation (c) Electrophoresis (d) Inversion
Last Answer : Denaturation
Description : Which of the following gives a positive Ninhydrin test? (A) Reducing sugar (B) Triglycerides (C) α-amino acids (D) Phospholipids
Description : Ninhydrin reaction gives a purple colour and evolves CO2 with (A) Peptide bonds (B) Histamine (C) Ergothioneine (D) Aspargine
Description : Which of the followings gives a positive test for Ninhydrin? (A) Reducing sugars (B) Triglycerides (C) Alpha aminoacids (D) Esterified Fats
Description : Ninhydrin with evolution of CO2 forms a blue complex with (A) Peptide bond (B) α-Amino acids (C) Serotonin (D) Histamine
Description : What is the importance of ninhydrin reaction?
Last Answer : It is used for qualitative test and quantitative es- timation of amino acids. It is often used for detec- tion of amino acids in chromatography.
Description : What is ninhydrin reaction?
Last Answer : All amino acids when heated with ninhydrin will give a pink colour.
Description : What reagent is used to test for proteins?
Last Answer : Proteins are detected using Biuret reagent . This turns a mauve or purple colour when mixed with protein.
Description : Calorific value of proteins as determined in a bomb calorimeter is (A) 4 kcal/gm (B) 4.8 kcal/gm (C) 5.4 kcal/gm (D) 5.8 kcal/gm
Description : Which of the following is correct? (a) When a Grignard reagent reacts with a ketone, the addition product is a primary alcohol. (b) When a Grignard reagent reacts with a ketone, the addition product ... with a aldehyde, the addition product is a tertiary alcohol. (d) None of the above are correct.
Last Answer : None of the above are correct.
Description : Presence of arginine can be detected by (A) Sakaguchi reaction (B) Million-Nasse reaction (C) Hopkins-Cole reaction (D) Gas chromatography
Description : The sorting out of molecules according to size and shape may be adapted to protein purification in this technique: (A) Adsorption chromatography (B) Gel filtration chromatography (C) Paper chromatography (D) None of these
Description : This technique takes the advantage of the fact that each protein has different pH at which it is electrically neutral i.e., its isoelectric pH: (A) Isoelectric focussing (B) Immunoel Ectro Phoresis (C) Chromatography (D) HPLC
Description : A sharp moving boundary is obtained between the pure solvent and solute containing layer in (A) Chromatography (B) Immuno Reactivity (C) Ultra Centrifugation (D) Solubility curve
Last Answer : Answer :C
Description : Give an example of affinity chromatography.
Last Answer : Separation and quantitation of glycated hemoglobin.
Description : Give the principle of affinity chromatography.
Last Answer : The technique is based on the high affinity of specific proteins for specific chemical groups.
Description : What is the principle of gel filtration chromatography?
Last Answer : The separation is effected on the basis of the size of the molecules. It is otherwise called molecular sieving.
Description : What is the basic principle of ion-exchange chromatography?
Last Answer : Here, the separation is based on electrostatic attraction between charged molecules to oppositely charged groups on the ion exchange resins.
Description : What is the advantage of TLC over paper chromatography?
Last Answer : TLC needs lesser time, and separation is more effective.
Description : What are the common types of partition chromatography?
Last Answer : Paper chromatography and thin layer chromatography.
Description : What is the principle of partition chromatography?
Last Answer : the components of the mixture to be separated are partitioned between the two phases depending on the partition co-efficient (solubility) of the particular substances.
Description : What is the principle of adsorption chromatography?
Last Answer : separation is based on differences in adsorption at the surface of a solid stationary medium.
Description : The linear arrangement of amino acid units in proteins is called : (a) primary structure (b) secondary structure (c) tertiary structure (d) quaternary structure
Last Answer : primary structure
Description : Which of the following is the first step in the determination of the primary structure of proteins? (a) determining the number and kind of amino acids in the peptide (b) reducing the disulfide bridges ... (c) protecting the N-terminal of the peptide (d) protecting the C-terminal of the peptide
Last Answer : reducing the disulfide bridges in the protein
Description : Lipoprotiens may be identified more accurately by means of (A) Electrophoresis (B) Ultra centrifugation (C) Centrifugation (D) Immunoelectrophoresis
Description : Plasma protein can be separated by (A) Salting out with (NH4)2SO4 (B) Ultracentrifugation (C) Immuno electrophoresis (D) All of these
Description : The antibodies raised against a pure protein will show only one sharp spike on this technique: (A) Solubility curve (B) Solvent precipitation (C) Molecular weight determination (D) Immuno electrophoresis
Description : Which one of the following statements concerning glutamine is correct? (A) Contains three tetratable groups (B) Is classified as an acidic amino acid (C) Contains an amide group (D) Migrates to the cathode during electrophoresis at pH 7.0