In sanger’s method of DNA sequence determination, DNA synthesis is stopped by using (A) 1′, 2′- Dideoxyribonucleoside triphosphates (B) 2′, 3′- Dideoxyribonucleoside triphosphates (C) 2′, 4′- Dideoxyribonucleoside triphosphates (D) 2′, 5′ - Dideoxyribonucleoside triphosphates

In sanger’s method of DNA sequence determination, DNA synthesis is stopped by using (A) 1′, 2′- Dideoxyribonucleoside triphosphates (B) 2′, 3′- Dideoxyribonucleoside triphosphates (C) 2′, 4′- Dideoxyribonucleoside triphosphates (D) 2′, 5′ - Dideoxyribonucleoside triphosphates
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Answer :  B
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Select the correct option. Direction of Direction of reading of RNA synthesis the template DNA strand (a) 5′ – 3′ 3′ – 5′ (b) 3′ – 5′ 5′ – 3′ (c) 5′ – 3′ 5′ – 3′ (d) 3′ – 5′ 3′ – 5′

Description : Select the correct option. Direction of Direction of reading of RNA synthesis the template DNA strand (a) 5′ – 3′ 3′ – 5′ (b) 3′ – 5′ 5′ – 3′ (c) 5′ – 3′ 5′ – 3′ (d) 3′ – 5′ 3′ – 5′

Last Answer : (d) 3′ – 5′ 3′ – 5′

Select the correct option. Direction of Direction of reading of RNA synthesis the template DNA strand (a) 5′ – 3′ 3′ – 5′ (b) 3′ – 5′ 5′ – 3′ (c) 5′ – 3′ 5′ – 3′ (d) 3′ – 5′ 3′ – 5′

Description : Select the correct option. Direction of Direction of reading of RNA synthesis the template DNA strand (a) 5′ – 3′ 3′ – 5′ (b) 3′ – 5′ 5′ – 3′ (c) 5′ – 3′ 5′ – 3′ (d) 3′ – 5′ 3′ – 5′

Last Answer : (a) 5′ – 3′ 3′ – 5′

Given below is a sample of a portion of DNA strand giving the base sequence on the opposite strands. What is so special shown in it? 5′ _____ GAATTC _____ 3′ 3′ _____ CTTAAG _____ 5′ (a) Replication completed (b) Deletion mutation (c) Start codon at the 5′ end (d) Palindromic sequence of base pairs

Description : Given below is a sample of a portion of DNA strand giving the base sequence on the opposite strands. What is so special shown in it? 5′ _____ GAATTC _____ 3′ 3′ _____ CTTAAG _____ 5′ (a) ... completed (b) Deletion mutation (c) Start codon at the 5′ end (d) Palindromic sequence of base pairs

Last Answer : (d) Palindromic sequence of base pairs

Which one of the following represents a palindromic sequence in DNA? (a) 5′ - GAATTC - 3′ (b) 5′ - CCAATG - 3′ 3′ - CTTAAG - 5′ 3′ - GAATCC - 5′ (c) 5′ - CATTAG - 3′ (d) 5′ - GATACC - 3′ 3′ - GATAAC - 5′ 3′ - CCTAAG - 5′

Description : Which one of the following represents a palindromic sequence in DNA? (a) 5′ - GAATTC - 3′ (b) 5′ - CCAATG - 3′ 3′ - CTTAAG - 5′ 3′ - GAATCC - 5′ (c) 5′ - CATTAG - 3′ (d) 5′ - GATACC - 3′ 3′ - GATAAC - 5′ 3′ - CCTAAG - 5′

Last Answer : (a) 5′ - GAATTC - 3′ 5′ - CCAATG - 3′ 3′ - CTTAAG - 5′

What will be the sequence of mRNA produced by the following stretch of DNA? 3′ATGCATGCATGCATG5′TEMPLATE STRAND 5′ TACGTACGTACGTAC3′ CODING STRAND (a) 3′AUGCAUGCAUGCAUG5′ (b) 5′UACGUACGUACGUAC 3′ (c) 3′ UACGUACGUACGUAC 5′ (d) 5′ AUGCAUGCAUGCAUG 3

Description : What will be the sequence of mRNA produced by the following stretch of DNA? 3′ATGCATGCATGCATG5′TEMPLATE STRAND 5′ TACGTACGTACGTAC3′ CODING STRAND (a) 3′AUGCAUGCAUGCAUG5′ (b) 5′UACGUACGUACGUAC 3′ (c) 3′ UACGUACGUACGUAC 5′ (d) 5′ AUGCAUGCAUGCAUG 3

Last Answer : d) 5′ AUGCAUGCAUGCAUG 3′

Direction of RNA synthesis is (A) 5′ → 3’ (B) 3′ → 5’ (C) Both (A) and (B) (D) None of these

Description : Direction of RNA synthesis is (A) 5′ → 3’ (B) 3′ → 5’ (C) Both (A) and (B) (D) None of these

Last Answer : Answer : A

All pribnow boxes are variants of the sequence: (A) 5′–TATAAT –3′ (B) 5′–GAGCCA –3′ (C) 5′–UAACAA –3′ (D) 5′–TCCTAG –3′

Description : All pribnow boxes are variants of the sequence: (A) 5′–TATAAT –3′ (B) 5′–GAGCCA –3′ (C) 5′–UAACAA –3′ (D) 5′–TCCTAG –3′

Last Answer : Answer : A

Although each specific tRNA differs from the others in its sequence of nucleotides, all tRNA molecules contain a base paired stem that terminates in the sequence CCA at (A) 3′ Termini (B)5′ Termini (C) Anticodon arm (D) 53 ′′ -Termini

Description : Although each specific tRNA differs from the others in its sequence of nucleotides, all tRNA molecules contain a base paired stem that terminates in the sequence CCA at (A) 3′ Termini (B)5′ Termini (C) Anticodon arm (D) 53 ′′ -Termini

Last Answer : Answer : A

mRNA is complementary copy of (A) 5′-3′ strand of DNA+ (B) 3′-5′ strand of DNA (C) Antisense strand of DNA (D) tRNA

Description : mRNA is complementary copy of (A) 5′-3′ strand of DNA+ (B) 3′-5′ strand of DNA (C) Antisense strand of DNA (D) tRNA

Last Answer : Answer : B

The specific palindromic sequence which is recognised by EcoRI is (a) 5′ - GAATTC - 3′ (b) 5′ - GGAACC - 3′ 3′ - CTTAAG - 5′ 3′ - CCTTGG - 5′ (c) 5′ - CTTAAG - 3′ (d) 5′ - GGATCC - 3′ 3′ - GAATTC - 5′ 3′ - CCTAGG - 5′.

Description : The specific palindromic sequence which is recognised by EcoRI is (a) 5′ - GAATTC - 3′ (b) 5′ - GGAACC - 3′ 3′ - CTTAAG - 5′ 3′ - CCTTGG - 5′ (c) 5′ - CTTAAG - 3′ (d) 5′ - GGATCC - 3′ 3′ - GAATTC - 5′ 3′ - CCTAGG - 5′.

Last Answer : (a) 5′ - GAATTC - 3′

Base sequence of DNA can be determined by (A) Maxam-Gilbert method (B) Sanger’s dideoxy method (C) Both (A) and (B) (D) None of these

Description : Base sequence of DNA can be determined by (A) Maxam-Gilbert method (B) Sanger’s dideoxy method (C) Both (A) and (B) (D) None of these

Last Answer : Answer : C

mRNA is synthesised on DNA template in which direction? (a) 5′ → 3′ (b) 3′ → 5′ (c) Both (a) and (b) (d) Any

Description : mRNA is synthesised on DNA template in which direction? (a) 5′ → 3′ (b) 3′ → 5′ (c) Both (a) and (b) (d) Any

Last Answer : a) 5′ → 3′

mRNA is synthesised on DNA template in which direction? (a) 5′ → 3′ (b) 3′ → 5′ (c) Both (a) and (b) (d) Any

Description : mRNA is synthesised on DNA template in which direction? (a) 5′ → 3′ (b) 3′ → 5′ (c) Both (a) and (b) (d) Any

Last Answer : (a) 5′ → 3′

n the DNA molecule, (a) the proportion of adenine in relation to thymine varies with the organism (b) there are two strands which run anti- parallel-one in 5′ → 3′ direction and other in 3′ → 5′ (c) the total amount of purine nucleotides and pyrimidine nucleotides is not always equal (d) there are two strands which run parallel in the 5′ → 3′ direction.

Description : n the DNA molecule, (a) the proportion of adenine in relation to thymine varies with the organism (b) there are two strands which run anti- parallel-one in 5′ → 3′ direction and other in 3′ → ... is not always equal (d) there are two strands which run parallel in the 5′ → 3′ direction.

Last Answer : (b) there are two strands which run anti- parallel-one in 5′ → 3′ direction and other in 3′ → 5′

The 3′ - 5′ phosphodiester linkages inside a polynucleotide chain serve to join (a) one DNA strand with the other DNA strand (b) one nucleoside with another nucleoside (c) one nucleotide with another nucleotide (d) one nitrogenous base with pentose sugar.

Description : The 3′ - 5′ phosphodiester linkages inside a polynucleotide chain serve to join (a) one DNA strand with the other DNA strand (b) one nucleoside with another nucleoside (c) one nucleotide with another nucleotide (d) one nitrogenous base with pentose sugar.

Last Answer : (c) one nucleotide with another nucleotide

All of the following statements about post-transcriptional processing of tRNA are true except (A) Introns of some tRNA precursors are removed (B) CCA is added at 3′ end (C) 7-Methylguanosine triphosphate cap is added at 5′ end (D) Some bases are methylated

Description : All of the following statements about post-transcriptional processing of tRNA are true except (A) Introns of some tRNA precursors are removed (B) CCA is added at 3′ end (C) 7-Methylguanosine triphosphate cap is added at 5′ end (D) Some bases are methylated

Last Answer : Answer : C

The degradation of RNA by pancreatic ribonuclease produces (A) Nucleoside 2-Phosphates (B) Nucleoside 5′-phosphates (C) Oligonucleosides (D) Nucleoside 3′-phosphate and oligonucleotide

Description : The degradation of RNA by pancreatic ribonuclease produces (A) Nucleoside 2-Phosphates (B) Nucleoside 5′-phosphates (C) Oligonucleosides (D) Nucleoside 3′-phosphate and oligonucleotide

Last Answer : Answer : D

In addition to Taq polymerase, polymerase chain reaction requires all of the following except (A) A template DNA (B) Deoxyribonucleoside triphosphates (C) Primers (D) Primase

Description : In addition to Taq polymerase, polymerase chain reaction requires all of the following except (A) A template DNA (B) Deoxyribonucleoside triphosphates (C) Primers (D) Primase

Last Answer : Answer : D

Deoxyribonucleotides are formed by reduction of (A) Ribonucleosides (B) Ribonucleoside monophosphates (C) Ribonucleoside diphosphates (D) Ribonucleoside triphosphates

Description : Deoxyribonucleotides are formed by reduction of (A) Ribonucleosides (B) Ribonucleoside monophosphates (C) Ribonucleoside diphosphates (D) Ribonucleoside triphosphates

Last Answer : Answer : C

N-terminal amino acids of a polypeptide are estimated by (A) Edmann reaction (B) Sanger’s reagent (C) Formaldehyde test (D) Ninhydrine reaction

Description : N-terminal amino acids of a polypeptide are estimated by (A) Edmann reaction (B) Sanger’s reagent (C) Formaldehyde test (D) Ninhydrine reaction

Last Answer : Answer : A

Sanger’s reagent contains (A) Phenylisothiocyanate (B) Dansyl chloride (C) 1-Fluoro-2, 4-dinitrobenzene (D) Ninhydrin

Description : Sanger’s reagent contains (A) Phenylisothiocyanate (B) Dansyl chloride (C) 1-Fluoro-2, 4-dinitrobenzene (D) Ninhydrin

Last Answer : Answer : C

Primary structure of proteins can be determined by the use of (A) Electrophoresis (B) Chromatography (C) Ninhydrin (D) Sanger’s reagent

Description : Primary structure of proteins can be determined by the use of (A) Electrophoresis (B) Chromatography (C) Ninhydrin (D) Sanger’s reagent

Last Answer : Answer : D

Termination of the synthesis of the RNA molecule is signaled by a sequence in the template strand of the DNA molecule, a signal that is recognized by a termination protein, the (A) Rho (ρ) factor (B) σ factor (C) δ factor (D) ε factor

Description : Termination of the synthesis of the RNA molecule is signaled by a sequence in the template strand of the DNA molecule, a signal that is recognized by a termination protein, the (A) Rho (ρ) factor (B) σ factor (C) δ factor (D) ε factor

Last Answer : Answer : A

Amniocentesis is a method for : (1) determination of foetal health conditions (2) determination of the amino acids sequence (3) inducing abortion (4) artificial insemination

Description : Amniocentesis is a method for : (1) determination of foetal health conditions (2) determination of the amino acids sequence (3) inducing abortion (4) artificial insemination

Last Answer : determination of foetal health conditions

What is antisense technology? (a) When a piece of RNA that is complementary in sequence is used to stop expression of a specific gene (b) RNA polymerase producing DNA (c) A cell displaying a foreign antigen used for synthesis of antigens (d) Production of somaclonal variants in tissue cultures

Description : What is antisense technology? (a) When a piece of RNA that is complementary in sequence is used to stop expression of a specific gene (b) RNA polymerase producing DNA (c) A cell displaying a foreign antigen used for synthesis of antigens (d) Production of somaclonal variants in tissue cultures

Last Answer : (a) When a piece of RNA that is complementary in sequence is used to stop expression of a specific gene

Which of the following is the correct sequence of events in the origin of life? I. Formation of protobionts II. Synthesis of organic monomers III. Synthesis of organic polymers IV. Formation of DNA-based genetic systems (a) I, II, III, IV (b) I, III, II, IV (c) II, III, I, IV (d) II, III, IV, I

Description : Which of the following is the correct sequence of events in the origin of life? I. Formation of protobionts II. Synthesis of organic monomers III. Synthesis of organic polymers IV. Formation of DNA-based genetic systems (a) I, II, III, IV (b) I, III, II, IV (c) II, III, I, IV (d) II, III, IV, I

Last Answer : c) II, III, I, IV

Which of the following pairs is wrongly matched? (a) Starch synthesis in pea : Multiple alleles (b) ABO blood grouping : Co-dominance (c) XO type sex determination: Grasshopper (d) T.H. Morgan : Linkage

Description : Which of the following pairs is wrongly matched? (a) Starch synthesis in pea : Multiple alleles (b) ABO blood grouping : Co-dominance (c) XO type sex determination: Grasshopper (d) T.H. Morgan : Linkage

Last Answer : (a) Starch synthesis in pea : Multiple alleles

Which of the following pairs in wrongly matched ? (1) Starch synthesis in pea : Multiple alleles (2) ABO blood grouping : Co-dominance (3) XO type sex determination : Grasshopper (4) T.H. Morgan : Linkage

Description : Which of the following pairs in wrongly matched ? (1) Starch synthesis in pea : Multiple alleles (2) ABO blood grouping : Co-dominance (3) XO type sex determination : Grasshopper (4) T.H. Morgan : Linkage

Last Answer : (1) Starch synthesis in pea : Multiple alleles

The signal sequence of proteins is cleaved off (A) On the ribosomes immediately after synthesis (B) In the endoplasmic reticulum (C) During processing in Golgi apparatus (D) During passage through the cell membrane

Description : The signal sequence of proteins is cleaved off (A) On the ribosomes immediately after synthesis (B) In the endoplasmic reticulum (C) During processing in Golgi apparatus (D) During passage through the cell membrane

Last Answer : Answer : B

The proteins destined to be transported out of the cell have all the following features except (A) They possess a signal sequence (B) Ribosomes synthesizing them are bound to endoplasmic reticulum (C) After synthesis, they are delivered into Golgi apparatus (D) They are tagged with ubiquitin

Description : The proteins destined to be transported out of the cell have all the following features except (A) They possess a signal sequence (B) Ribosomes synthesizing them are bound to endoplasmic reticulum (C) After synthesis, they are delivered into Golgi apparatus (D) They are tagged with ubiquitin

Last Answer : Answer : D

The sequence of amino acids in human growth hormone and the synthesis were done by (A) Sanger (B) Krebs (C) Chah Holi (D) Molisch

Description : The sequence of amino acids in human growth hormone and the synthesis were done by (A) Sanger (B) Krebs (C) Chah Holi (D) Molisch

Last Answer : Answer : C

Template-directed DNA synthesis occurs in all the following except (A) The replication fork (B) Polymerase chain reaction (C) Growth of RNA tumor viruses (D) Expression of oncogenes

Description : Template-directed DNA synthesis occurs in all the following except (A) The replication fork (B) Polymerase chain reaction (C) Growth of RNA tumor viruses (D) Expression of oncogenes

Last Answer : Answer : C

The mechanism of synthesis of DNA and RNA are similar to all the following ways except (A) They involve release of pyrophosphate from each nucleotide added (B) They require activated nucleotide precursor and Mg2+ (C) The direction of synthesis is (D) They require a primer

Description : The mechanism of synthesis of DNA and RNA are similar to all the following ways except (A) They involve release of pyrophosphate from each nucleotide added (B) They require activated nucleotide precursor and Mg2+ (C) The direction of synthesis is (D) They require a primer

Last Answer : Answer : D

Amanitin the mushroom poison inhibits (A) Glycoprotein synthesis (B) ATP synthesis (C) DNA synthesis (D) mRNA synthesis

Description : Amanitin the mushroom poison inhibits (A) Glycoprotein synthesis (B) ATP synthesis (C) DNA synthesis (D) mRNA synthesis

Last Answer : Answer : D

Translocase is an enzyme required in the process of (A) DNA replication (B) RNA synthesis (C) Initiation of protein synthesis (D) Elongation of peptides

Description : Translocase is an enzyme required in the process of (A) DNA replication (B) RNA synthesis (C) Initiation of protein synthesis (D) Elongation of peptides

Last Answer : Answer : D

Which one of the following statements correctly describes eukaryotic DNA? (A) They involve release of pyrophosphate from each nucleotide precussor and Mg2+ (B) The direction of synthesis is (C) They require a primer 5’→ 3’ (D) None of these

Description : Which one of the following statements correctly describes eukaryotic DNA? (A) They involve release of pyrophosphate from each nucleotide precussor and Mg2+ (B) The direction of synthesis is (C) They require a primer 5’→ 3’ (D) None of these

Last Answer : Answer : C

Template-directed DNA synthesis occurs in all the following except (A) The replication fork (B) Polymerase chain reaction (C) Growth of RNA tumor viruses (D) Expression of oneogenes

Description : Template-directed DNA synthesis occurs in all the following except (A) The replication fork (B) Polymerase chain reaction (C) Growth of RNA tumor viruses (D) Expression of oneogenes

Last Answer : Answer : C

The mechanism of synthesis of DNA and RNA are similar in all the following ways except (A) They involve release of pyrophosphate from each nucleotide added (B) They require activated nucleotide precursor and Mg2+ (C) The direction of synthesis is 5’ → 3’ (D) They require a primer

Description : The mechanism of synthesis of DNA and RNA are similar in all the following ways except (A) They involve release of pyrophosphate from each nucleotide added (B) They require activated nucleotide precursor and Mg2+ (C) The direction of synthesis is 5’ → 3’ (D) They require a primer

Last Answer : Answer : A

RNA synthesis requires (A) RNA primer (B) RNA template (C) DNA template (D) DNA primer

Description : RNA synthesis requires (A) RNA primer (B) RNA template (C) DNA template (D) DNA primer

Last Answer : Answer : C

DNA synthesis in laboratory was first achieved by (A) Watson and crick (B) Khorana (C) A.Kornberg (D) Ochoa

Description : DNA synthesis in laboratory was first achieved by (A) Watson and crick (B) Khorana (C) A.Kornberg (D) Ochoa

Last Answer : Answer : C

Ciprofloxacin inhibits the synthesis of (A) DNA in prokaryotes (B) DNA in prokaryotes and eukaryotes (C) RNA in prokaryotes (D) RNA in prokaryotes and eukaryotes

Description : Ciprofloxacin inhibits the synthesis of (A) DNA in prokaryotes (B) DNA in prokaryotes and eukaryotes (C) RNA in prokaryotes (D) RNA in prokaryotes and eukaryotes

Last Answer : Answer : A

Ciprofloxacin inhibits the synthesis of (A) DNA (B) mRNA (C) tRNA (D) rRNA

Description : Ciprofloxacin inhibits the synthesis of (A) DNA (B) mRNA (C) tRNA (D) rRNA

Last Answer : Answer : A

Novobicin inhibits the synthesis of (A) DNA (B) mRNA (C) tRNA (D) rRNA

Description : Novobicin inhibits the synthesis of (A) DNA (B) mRNA (C) tRNA (D) rRNA

Last Answer : Answer : A

Synthesis of RNA and a DNA template is known as (A) Replication (B) Translation (C) Transcription (D) Mutation

Description : Synthesis of RNA and a DNA template is known as (A) Replication (B) Translation (C) Transcription (D) Mutation

Last Answer : Answer : C

In mammalian cell cycle, synthesis of DNA occurs during (A) S phase (B) G1 phase (C) Mitotic Phase (D) G2 phase

Description : In mammalian cell cycle, synthesis of DNA occurs during (A) S phase (B) G1 phase (C) Mitotic Phase (D) G2 phase

Last Answer : Answer : A

Reverse transcriptase catalyses (A) Synthesis of RNA (B) Breakdown of RNA (C) Synthesis of DNA (D) Breakdown of DNA

Description : Reverse transcriptase catalyses (A) Synthesis of RNA (B) Breakdown of RNA (C) Synthesis of DNA (D) Breakdown of DNA

Last Answer : Answer : C

Okazaki pieces are formed during the synthesis of (A) mRNA (B) tRNA (C) rRNA (D) DNA

Description : Okazaki pieces are formed during the synthesis of (A) mRNA (B) tRNA (C) rRNA (D) DNA

Last Answer : Answer : D

Direction of DNA synthesis is (A) 5’ → 3’ (B) 3’ → 5’ (C) Both (A) and (B) (D) None of these

Description : Direction of DNA synthesis is (A) 5’ → 3’ (B) 3’ → 5’ (C) Both (A) and (B) (D) None of these

Last Answer : Answer : A

Synthesis of DNA is also known as (A) Duplication (B) Replication (C) Transcription (D) Translation

Description : Synthesis of DNA is also known as (A) Duplication (B) Replication (C) Transcription (D) Translation

Last Answer : Answer : B

Okazaki fragment is related to (A) DNA synthesis (B) Protein synthesis (C) mRNA formation (D) tRNA formation

Description : Okazaki fragment is related to (A) DNA synthesis (B) Protein synthesis (C) mRNA formation (D) tRNA formation

Last Answer : Answer : A