What are the benefits of protein expression and purification?

What are the benefits of protein expression and purification?
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Protein could be expressed in various systems and each system has its own advantages. To choose which system, we should take several factors into consideration such as the Cost, the type of protein, function and desired protein yield. The most widely used systems are expressions in Bacterial, Yeast, Insect and Mammalian.
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What does protein expression and purification mean?

Description : What does protein expression and purification mean?

Last Answer : Protein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, usually cells, tissues or whole organisms. Protein purification is vital for ... the importance of building a convenient and easy to use protein high expression and purification system.

Is protein expression and purification a scientific process?

Description : Is protein expression and purification a scientific process?

Last Answer : Yes. Protein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, usually cells, tissues or whole organisms. Protein purification is vital ... vital for the characterization of the function, structure and interactions of the protein of interest.

Which of the following is not a component of downstream processing? (a) Separation (b) Purification (c) Preservation (d) Expression

Description : Which of the following is not a component of downstream processing? (a) Separation (b) Purification (c) Preservation (d) Expression

Last Answer : (d) Expression

The sorting out of molecules according to size and shape may be adapted to protein purification in this technique: (A) Adsorption chromatography (B) Gel filtration chromatography (C) Paper chromatography (D) None of these

Description : The sorting out of molecules according to size and shape may be adapted to protein purification in this technique: (A) Adsorption chromatography (B) Gel filtration chromatography (C) Paper chromatography (D) None of these

Last Answer : Answer : B

The technique for purification of proteins that can be made specific for a given protein is (A) Gel filtration chromotography (B) Ion exchange chromatography (C) Electrophoresis (D) Affinity chromatography

Description : The technique for purification of proteins that can be made specific for a given protein is (A) Gel filtration chromotography (B) Ion exchange chromatography (C) Electrophoresis (D) Affinity chromatography

Last Answer : Answer : D

The process of separation and purification of expressed protein before marketing is called (a) downstream processing (b) bioprocessing (c) postproduction processing (d) upstream processing.

Description : The process of separation and purification of expressed protein before marketing is called (a) downstream processing (b) bioprocessing (c) postproduction processing (d) upstream processing.

Last Answer : (a) downstream processing

The process of separation and purification of expressed protein before marketing is called : (1) Downstream processing (2) Bioprocessing (3) Postproduction processing (4) Upstream processing

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Air purification: Why does the air in nice hotels feel so good?

Description : Air purification: Why does the air in nice hotels feel so good?

Last Answer : To me, 67 degrees is chilly enough that it would have woken me up.

Will you help me choose my home water purification plan?

Description : Will you help me choose my home water purification plan?

Last Answer : answer:I like aquasana products because they restrict the flow much less than others I've tried, and we have lousy water pressure (~35psi). The least invasive would be their countertop system, which ... what I've read you are correct that de-mineralized water will pull vital minerals out of you.

Anybody here familiar with water purification calculations?

Description : Anybody here familiar with water purification calculations?

Last Answer : All I can tell you is that Spellman is a textbook, and the pages involved can be found online here. It includes sample calculations as well as the definition of terms such as residual.

Which oil is used in the floatation method for the purification of ores? -General Knowledge

Description : Which oil is used in the floatation method for the purification of ores? -General Knowledge

Last Answer : The answer is 'Pine oil'

Purification of petroleum is carried out by which method? -General Knowledge

Description : Purification of petroleum is carried out by which method? -General Knowledge

Last Answer : The answer is 'Fractional distillation'

Which material is suitable for water purification? -Do You Know?

Description : Which material is suitable for water purification? -Do You Know?

Last Answer : answer:

Purification of petroleum is carried out by which method? -General Knowledge

Description : Purification of petroleum is carried out by which method? -General Knowledge

Last Answer : answer:

Purification of petroleum is carried out by which method? -General Knowledge

Description : Purification of petroleum is carried out by which method? -General Knowledge

Last Answer : answer:

What is water purification ?

Description : What is water purification ?

Last Answer : Water purification is the process of purifying contaminated water in a specific process. Water can contain a variety of contaminants. There are several ways to purify water Below they are highlighted: 1. ... filter method. 3. Chemical methods: - Chlorination. 4. Ultraviolet rays. 5. Boiling water.

What is the name of water purification tablet ?

Last Answer : : The name of the water purification tablet is Halogen.

Sewage purification is performed by

Description : Sewage purification is performed by

Last Answer : Sewage purification is performed by A. Microbes B. Fertilizers C. Antibiotics D. Antiseptics

Which method of purification is represented by the following equations `Ti +2I_(2) overset(523K)rarr TiI_(4) overset(1700K)rarr Ti +2I_(2)`

Description : Which method of purification is represented by the following equations `Ti +2I_(2) overset(523K)rarr TiI_(4) overset(1700K)rarr Ti +2I_(2)`

Last Answer : Which method of purification is represented by the following equations `Ti +2I_(2) overset(523K)rarr TiI_( ... B. Poling C. Can arkel D. zone refining

Van Arkel method of purification of metals involves converting the metal to

Description : Van Arkel method of purification of metals involves converting the metal to

Last Answer : Van Arkel method of purification of metals involves converting the metal to A. Volatile ... stable compound D. non-volatile unstable compound

In the froth floatation process for the purification of minerals the particles float because

Description : In the froth floatation process for the purification of minerals the particles float because

Last Answer : In the froth floatation process for the purification of minerals the particles float because A. they ... by oil D. They bear an electrostatic charge.

(xiv) Following equation represents a method of purification of nickel by `underset ("Impure")(Ni) + 4CO overset (320 K)rarr Ni(CO)_(4) overset(420 K)

Description : (xiv) Following equation represents a method of purification of nickel by `underset ("Impure")(Ni) + 4CO overset (320 K)rarr Ni(CO)_(4) overset(420 K)

Last Answer : (xiv) Following equation represents a method of purification of nickel by `underset ("Impure")(Ni) + ... Arkel B. Zone refining C. Mond D. Cupellation

Van Arkel method of purification of metals involves converting the metal to

Description : Van Arkel method of purification of metals involves converting the metal to

Last Answer : Van Arkel method of purification of metals involves converting the metal to A. Volatile stable ... . Volatile unstable compound D. None of these

In which of the following method of purification metal is converted to its volatile compound which is decomposed to give pure metal?

Description : In which of the following method of purification metal is converted to its volatile compound which is decomposed to give pure metal?

Last Answer : In which of the following method of purification metal is converted to its volatile compound ... Heating with iodine C. Liquidation D. Distillation

If a metal has low oxygen affinity then the purification of metal may be carried out by :

Description : If a metal has low oxygen affinity then the purification of metal may be carried out by :

Last Answer : If a metal has low oxygen affinity then the purification of metal may be carried out by : A. liquation B. distillation C. zone refining D. cupellation

If the impurity in a metal has a greater affinity for oxygen and is more easily oxidised than the metal, then the purification of metal may be carried

Description : If the impurity in a metal has a greater affinity for oxygen and is more easily oxidised than the metal, then the purification of metal may be carried

Last Answer : If the impurity in a metal has a greater affinity for oxygen and is more easily oxidised than ... B. electrorefining C. Zone refining D. POILING

Are water softeners and water purification systems the same?

Description : Are water softeners and water purification systems the same?

Last Answer : Yes, there is a difference between water softeners and water purification systems as water softeners merely filter the water whereas water purification systems actually filter and cleanse the water.

For water purification, we use _____. A. Chlorine B. Flourine C. Bromine D. Lodine

Description : For water purification, we use _____. A. Chlorine B. Flourine C. Bromine D. Lodine

Last Answer : ANSWER: A

For water purification, we use _____. A. Chlorine B. Flourine C. Bromine D. Lodine

Description : For water purification, we use _____. A. Chlorine B. Flourine C. Bromine D. Lodine

Last Answer : ANSWER: A

For water purification, we use _____. A. Chlorine B. Flourine C. Bromine D. Lodine

Description : For water purification, we use _____. A. Chlorine B. Flourine C. Bromine D. Lodine

Last Answer : ANSWER: A

Ultra purification of a metal is done by (1) slugging (2) zone melting (3) smelting (4) leaching

Description : Ultra purification of a metal is done by (1) slugging (2) zone melting (3) smelting (4) leaching

Last Answer : (2) zone melting Explanation: When metals are required in a ultra pure state, the zone refining method is used. The principleemployed states that the impurities, which lower the melting point of ... remain preferentially dissolved in the liquid phase and purer metal will emerge in the solid phase.

Muddy water is treated with alum in purification process, it is termed as : (1) emulsification (2) absorption (3) adsorption (4) coagulation

Description : Muddy water is treated with alum in purification process, it is termed as : (1) emulsification (2) absorption (3) adsorption (4) coagulation

Last Answer : (4) coagulation Explanation: Natural and wastewater contain small particulates that are suspended in water forming a colloid. These particles carry the same charges, and repulsion prevents them from combining into larger particulates to settle.

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Description : During purification process of sewage the gas given off, is A. Carbon dioxide B. Hydrogen C. Ammonia D. All the above

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Disposal to sewage in large cities, is done in A. Irrigation B. Dilution C. Oxidation D. Purification

Description : Disposal to sewage in large cities, is done in A. Irrigation B. Dilution C. Oxidation D. Purification

Last Answer : ANS: B

The factor responsible for purification of sewage in river is A. Hydrology B. Dissolved oxygen in water C. Temperature D. All the above

Description : The factor responsible for purification of sewage in river is A. Hydrology B. Dissolved oxygen in water C. Temperature D. All the above

Last Answer : ANS: D

54 Pick up the correct statement from the following: A. The ratio of the quantity of the diluting water to that of the sewage, is known as dilution factor B. The automatic purification of polluted water, is known self purification phenomenon C. The photosynthesis is carried out in the presence of sun light D. All the above

Description : 54 Pick up the correct statement from the following: A. The ratio of the quantity of the diluting water to that of the sewage, is known as dilution factor B. The automatic purification of polluted ... phenomenon C. The photosynthesis is carried out in the presence of sun light D. All the above

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Ultra purification of a Metal is done by–

Description : Ultra purification of a Metal is done by–

Last Answer : Zone Melting

Purification of copper is done by –

Description : Purification of copper is done by –

Last Answer : E l e c t r o l y t i c decomposition

Zone refining is used for the purification of?

Description : Zone refining is used for the purification of?

Last Answer : Ge

Which material is suitable for water purification?

Description : Which material is suitable for water purification?

Last Answer : Zeolites

Which is used for the purification of gold?

Description : Which is used for the purification of gold?

Last Answer : Nitric acid (Spirit of Nitre)

A liquid with high boiling point decomposes on simple distillation but it can be steam distilled for its purification .Explain how is it possible?

Description : A liquid with high boiling point decomposes on simple distillation but it can be steam distilled for its purification .Explain how is it possible?

Last Answer : In steam distillation boiling occurs when vapour pressure of organic compound & steam becomes equal to atmospheric pressure. As vapour pressure of water is higher than the organic liquid. ... organic liquid vaporizes at a temperature below its boiling point in steam distillation without decomposing.

Pick out the correct statement. (A) Dialysis process is used for the production of fresh water from brine (B) Electrodialysis is used in the manufacture of NaOH (C) Electrodialysis is used for the refining/purification of petrol (D) Both (B) and (C)

Description : Pick out the correct statement. (A) Dialysis process is used for the production of fresh water from brine (B) Electrodialysis is used in the manufacture of NaOH (C) Electrodialysis is used for the refining/purification of petrol (D) Both (B) and (C)

Last Answer : (B) Electrodialysis is used in the manufacture of NaOH

Dialysis is a method of purification of sols. But prolonged dialysis of the sol makes it unstable. Why ?

Description : Dialysis is a method of purification of sols. But prolonged dialysis of the sol makes it unstable. Why ?

Last Answer : Ans. Traces of electrolytes in the sol, impart charge to dispersed phase particles making it stable. Prolonged dialysis removes all electrolytes thus making the sol unstable.

For water purification, we use? A. Chlorine (Answer) B. Bromine C. Plants D. Young man

Description : For water purification, we use? A. Chlorine (Answer) B. Bromine C. Plants D. Young man

Last Answer : A. Chlorine (Answer)

What is the purpose of TOPP (turbine oil purification plant)?

Description : What is the purpose of TOPP (turbine oil purification plant)? 

Last Answer : The purpose of TOPP is to remove the water ingress in turbine oil system from the gland leaks, cooler leakage, and solid metal particles, which are produced due to rust, wear of bearings and to normalize the ... capacity of TOPP is, for solids - 5 microns and for water - 300 to 500 parts per milli.

How turbine oil purification is achieved?

Description : How turbine oil purification is achieved?

Last Answer : Oil purification is achieved by centrifugal operation.  

Stirred-tank bioreactors have been designed for (a) addition of preservatives to the product (b) purification of the product (c) ensuring anaerobic conditions in the culture vessel (d) availability of oxygen throughout the process.

Description : Stirred-tank bioreactors have been designed for (a) addition of preservatives to the product (b) purification of the product (c) ensuring anaerobic conditions in the culture vessel (d) availability of oxygen throughout the process.

Last Answer : (d) availability of oxygen throughout the process.

Stirred-tank bioreactors have been designed for (a) purification of product (b) addition of preservatives to the product (c) availability of oxygen throughout the process (d) ensuring anaerobic conditions in the culture vessel.

Description : Stirred-tank bioreactors have been designed for (a) purification of product (b) addition of preservatives to the product (c) availability of oxygen throughout the process (d) ensuring anaerobic conditions in the culture vessel.

Last Answer : (c) availability of oxygen throughout the process

Aerobic biological oxidation ponds used for the purification of polluted water (A) Destroys/removes pathogen from the sewage (B) Is not very effective for non-biodegradable substances (e.g. ABS) containing effluents (C) Destroys/removes pathogen much more effectively if the sewage is chlorinated (D) All (A), (B) & (C)

Description : Aerobic biological oxidation ponds used for the purification of polluted water (A) Destroys/removes pathogen from the sewage (B) Is not very effective for non-biodegradable substances (e.g. ABS) containing effluents (C) ... much more effectively if the sewage is chlorinated (D) All (A), (B) & (C)

Last Answer : (D) All (A), (B) & (C)