What is fluorescence ?

What is fluorescence ?
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1 Answer

Answer :

: Substances that absorb the 200-375 nanometer wavelength spectrum of the UV region but emit visible spectra with a wavelength of 400-780 nanometers are called fluorescence substances.
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Related questions

The fluorescence of the glass (discharge) tube at very low pressure is characteristic of_________.

Description : The fluorescence of the glass (discharge) tube at very low pressure is characteristic of_________.

Last Answer : The fluorescence of the glass (discharge) tube at very low pressure is characteristic of_________. A. ... tube C. the cathode D. the emitted particles

What is immuno fluorescence ?

Description : What is immuno fluorescence ?

Last Answer : Fluorescence is the property of absorbing light ray of particular wavelength and emitting rays in different wavelength. Antigens that are bound to cells or tissue sections can be visualized by tugging the antibody molecule with a fluorescent dye or fluorochrome.

Into how many types is immuno fluorescence is divided ?

Description : Into how many types is immuno fluorescence is divided ?

Last Answer : Immuno fluorescence is divided into 2 types- Direct immuno fluorescence Indirect immuno fluorescence

What is direct immuno fluorescence ?

Description : What is direct immuno fluorescence ?

Last Answer : In this method, the species antibodies are primary antibodies, which are directly conjugated to fluorescent dye.

What is the disadvantage of direct immuno fluorescence ?

Description : What is the disadvantage of direct immuno fluorescence ?

Last Answer : A separate fluorescent conjugate have to be prepared against each antigen to be tested.

What is indirect immuno fluorescence ?

Description : What is indirect immuno fluorescence ?

Last Answer : In a method the primary unlabelled antibody is detected with a number of reagents have been developed for indirect staining. The most common is fluorescence labeled anti isotype antibody such as fluoroscin labeled goat- mouse antibody.

What are the advantages of indirect immuno fluorescence ?

Description : What are the advantages of indirect immuno fluorescence ?

Last Answer : The primary does not need to be conjugated with label. It increases the sensitivity of staining because multiple fluorochrome reagents will bind to each antibody molecule. This method has great flexibility.

What are the uses of indirect immuno fluorescence ?

Description : What are the uses of indirect immuno fluorescence ?

Last Answer : For identifying bacterial species Detecting antigen-antibody complexes in autoimmune diseases Detecting compliment components in tissues. Localizing hormones

When a metal is heated in a flame, the electrons absorb energy and jump to higher energy state. On coming back to the lower energy state, they emit light, which we can observe in - (1) Raman spectra (2) Absorption spectra (3) Emission spectra (4) Fluorescence

Description : When a metal is heated in a flame, the electrons absorb energy and jump to higher energy state. On coming back to the lower energy state, they emit light, which we can observe in - (1) Raman spectra (2) Absorption spectra (3) Emission spectra (4) Fluorescence

Last Answer : (3) Emission spectra Explanation: When a metal atom is strongly heated, its electrons absorb the heat energy and jump to a higher energy level. When the electron returns to its original position it ... to the energy the electron has released at each stage °fits fall back to its original state.

Pick out the wrong statement. (A) A pale color of petroleum product indicates lower viscosity (B) Color of petroleum products indicates the degree of refinement (C) Lighter petroleum distillates are lighter in color than the heavier residual oils (D) Fluorescence of oils helps to detect its adulteration

Description : Pick out the wrong statement. (A) A pale color of petroleum product indicates lower viscosity (B) Color of petroleum products indicates the degree of refinement (C) Lighter petroleum distillates are ... in color than the heavier residual oils (D) Fluorescence of oils helps to detect its adulteration

Last Answer : (A) A pale color of petroleum product indicates lower viscosity

Particle nature of cathode rays is proved due to the fact that they (A) Travel in a straight line (B) Get deflected by magnetic electric field (C) Produce fluorescence (D) Heat the exposed material

Description : Particle nature of cathode rays is proved due to the fact that they (A) Travel in a straight line (B) Get deflected by magnetic electric field (C) Produce fluorescence (D) Heat the exposed material

Last Answer : Option B

In organic molecules, fluorescence seldom results from absorption of UV radiation of wavelengths lower than (A) 350 nm (B) 200 nm (C) 300 nm (D) 250 nm

Description : In organic molecules, fluorescence seldom results from absorption of UV radiation of wavelengths lower than (A) 350 nm (B) 200 nm (C) 300 nm (D) 250 nm

Last Answer : (A) 350 nm

Indicate the HPLC detector that is most sensitive to change in temperature : (A) PDA detector (B) Refractive Index detector (C) Electrochemical detector (D) Fluorescence detector

Description : Indicate the HPLC detector that is most sensitive to change in temperature : (A) PDA detector (B) Refractive Index detector (C) Electrochemical detector (D) Fluorescence detector

Last Answer : (B) Refractive Index detector

Which one of the following statements is WRONG? (A) A singlet or triplet state may result when one of the electrons from the HOMO is excited to higher energy levels (B) In an excited singlet state, the spin of the electron in the higher energy orbital is paired with the electron in the ground state orbital (C) Triplet excited state is more stable than the singlet excited state (D) When the electron from the singlet excited state returns to ground state, the molecule always shows fluorescence phenomenon

Description : Which one of the following statements is WRONG? (A) A singlet or triplet state may result when one of the electrons from the HOMO is excited to higher energy levels (B) In an ... the electron from the singlet excited state returns to ground state, the molecule always shows fluorescence phenomenon

Last Answer : (C) Triplet excited state is more stable than the singlet excited state

Which of the following HPLC detectors is used as a bulk property or general purpose detector? A. Electrochemical detector B. Fluorescence detector C. UV-Visible detector D. Evaporative Light scattering detector

Description : Which of the following HPLC detectors is used as a bulk property or general purpose detector? A. Electrochemical detector B. Fluorescence detector C. UV-Visible detector D. Evaporative Light scattering detector

Last Answer : D. Evaporative Light scattering detector

Hydraulic lime is not used in plastering because it may result in a) Blistering* b) Efflorescence c) Flaking d) Fluorescence

Description : Hydraulic lime is not used in plastering because it may result in a) Blistering* b) Efflorescence c) Flaking d) Fluorescence

Last Answer : a) Blistering*

The fluorescence examination of the seminal stains indicates (a). Pink color (b). White color (c). Blue color (d). Red color

Description : The fluorescence examination of the seminal stains indicates (a). Pink color (b). White color (c). Blue color (d). Red color

Last Answer : Ans: (b). Blue color 

Which of the following is an example of bulk property or general detector in HPLC A. Fluorescence detector B. Refractive index detector C. Electrochemical detector D. UV-Visible detector

Description : Which of the following is an example of bulk property or general detector in HPLC A. Fluorescence detector B. Refractive index detector C. Electrochemical detector D. UV-Visible detector

Last Answer : Refractive index detector

When a metal is heated in a flame, the electrons absorb energy and jump to higher energy state. On coming back to the lower energy siate, they emit light, which we can observe in (1) Raman spectra (2) Absorption spectra (3) Emission spectra (4) Fluorescence

Description : When a metal is heated in a flame, the electrons absorb energy and jump to higher energy state. On coming back to the lower energy siate, they emit light, which we can observe in (1) Raman spectra (2) Absorption spectra (3) Emission spectra (4) Fluorescence

Last Answer : Emission spectra

What is phosphorescence and fluorescence?

Description : What is phosphorescence and fluorescence?

Last Answer : Fluorescence is the light produced when the source of energy is applied. The light produced after the removal of source of energy is known as phosphorescence.