Plasmid DNA isolation by alkaline lysis method. -Biology

Plasmid DNA isolation by alkaline lysis method. -Biology
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7. In a recombinant DNA technology a plasmid vector must be cleaved by a) the same enzyme that leaves the donor gene b) modified DNA ligase c) a heated alkaline solution d) four separate enzymes

Description : 7. In a recombinant DNA technology a plasmid vector must be cleaved by a) the same enzyme that leaves the donor gene b) modified DNA ligase c) a heated alkaline solution d) four separate enzymes

Last Answer : a) the same enzyme that leaves the donor gene

What is plasmid DNA? -Biology

Description : What is plasmid DNA? -Biology

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Where is plasmid DNA found? -Biology

Description : Where is plasmid DNA found? -Biology

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Difference between plasmid DNA and genomic DNA -Biology

Description : Difference between plasmid DNA and genomic DNA -Biology

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Plasmid vector in DNA recombinant technology means

Description : Plasmid vector in DNA recombinant technology means

Last Answer : Plasmid vector in DNA recombinant technology means A. a virus that transfers gene to bacteria B. ... D. any fragment of DNA carrying desirable gene

A foreign DNA and plasmid cut by the same restriction endonculease can be joined to form a recombinant plasmid using

Description : A foreign DNA and plasmid cut by the same restriction endonculease can be joined to form a recombinant plasmid using

Last Answer : A foreign DNA and plasmid cut by the same restriction endonculease can be joined to form a recombinant ... B. Ligase C. Eco RI D. Taq polymerase

A plasmid is a (A) Single stranded linear DNA (B) Single stranded circular DNA (C) Double stranded linear DNA (D) Double stranded circular DNA

Description : A plasmid is a (A) Single stranded linear DNA (B) Single stranded circular DNA (C) Double stranded linear DNA (D) Double stranded circular DNA

Last Answer : Answer : D

The process of replication in plasmid DNA, other than initiation, is controlled by (a) mitochondrial gene (b) plasmid gene (c) bacterial gene (d) none of these.

Description : The process of replication in plasmid DNA, other than initiation, is controlled by (a) mitochondrial gene (b) plasmid gene (c) bacterial gene (d) none of these.

Last Answer : c) bacterial gene

Plasmid has been used as vector because (a) it is circular DNA which have capacity to join to eukaryotic DNA (b) it can move between prokaryotic and eukaryotic cells (c) both ends show replication (d) it has antibiotic resistance gene.

Description : Plasmid has been used as vector because (a) it is circular DNA which have capacity to join to eukaryotic DNA (b) it can move between prokaryotic and eukaryotic cells (c) both ends show replication (d) it has antibiotic resistance gene.

Last Answer : (a) it is circular DNA which have capacity to join to eukaryotic DNA

The linking of antibiotic resistance gene with the plasmid vector became possible with (a) DNA polymerase (b) exonucleases (c) DNA ligase (d) endonucleases.

Description : The linking of antibiotic resistance gene with the plasmid vector became possible with (a) DNA polymerase (b) exonucleases (c) DNA ligase (d) endonucleases.

Last Answer : (c) DNA ligase

In genetic engineering, a DNA segment (gene) of interest, is transferred to the host cell through a vector. Consider the following four agents (i-iv) in this regard and select the correct option about which one or more of these can be used as a vector/vectors. (i) Bacterium (ii) Plasmid (iii)Plasmodium (iv) Bacteriophage (a) (i), (ii) and (iv) (b) (i) only (c) (i) and (iii) (d) (ii) and (iv)

Description : In genetic engineering, a DNA segment (gene) of interest, is transferred to the host cell through a vector. Consider the following four agents (i-iv) in this regard and select the correct option about which one or more of these ... ii) and (iv) (b) (i) only (c) (i) and (iii) (d) (ii) and (iv)

Last Answer : (d) (ii) and (iv)

DNA or RNA segment tagged with a radioactive molecule is called (a) vector (b) probe (c) clone (d) plasmid.

Description : DNA or RNA segment tagged with a radioactive molecule is called (a) vector (b) probe (c) clone (d) plasmid.

Last Answer : (b) probe

Which vector can clone only a small fragment of DNA? (a) Bacterial artificial chromosome (b) Yeast artificial chromosome (c) Plasmid (d) Cosmid

Description : Which vector can clone only a small fragment of DNA? (a) Bacterial artificial chromosome (b) Yeast artificial chromosome (c) Plasmid (d) Cosmid

Last Answer : (c) Plasmid

A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using (a) EcoRI (b) Taq polymerase (c) polymerase III (d) ligase.

Description : A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using (a) EcoRI (b) Taq polymerase (c) polymerase III (d) ligase.

Last Answer : (d) ligase.

Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes? (a) Retrovirus (b) Ti plasmid (c) λ phage (d) pBR322

Description : Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes? (a) Retrovirus (b) Ti plasmid (c) λ phage (d) pBR322

Last Answer : (a) Retrovirus

What are the sex organs provided in some bacteria? (a) Sex pili (b) Plasmid (c) Circular DNA (d) Gametes

Description : What are the sex organs provided in some bacteria? (a) Sex pili (b) Plasmid (c) Circular DNA (d) Gametes

Last Answer : (a) Sex pili

n bacteria, plasmid is (a) extra chromosomal material (b) main DNA (c) non functional DNA (d) repetitive gene.

Description : n bacteria, plasmid is (a) extra chromosomal material (b) main DNA (c) non functional DNA (d) repetitive gene.

Last Answer : (a) extra chromosomal material

In bacteria, plasmid is (a) extra chromosomal material (b) main DNA (c) non functional DNA (d) repetitive gene.

Description : In bacteria, plasmid is (a) extra chromosomal material (b) main DNA (c) non functional DNA (d) repetitive gene.

Last Answer : (a) extra chromosomal material

Which of the following defines an open reading frame (ORF)? A.A sequence of genome in bacteriophageB.The sequence of a complete genome C.A plasmid vector used in genome sequencing D.A possible gene predicted by DNA sequencing

Description : Which of the following defines an open reading frame (ORF)? A.A sequence of genome in bacteriophage B.The sequence of a complete genome C.A plasmid vector used in genome sequencing D.A possible gene predicted by DNA sequencing

Last Answer : D.A possible gene predicted by DNA sequencing

Which of the following defines an open reading frame (ORF)? A- .A sequence of genome in bacteriophage B- The sequence of a complete genome C- A plasmid vector used in genome sequencing D- .A possible gene predicted by DNA sequencing

Description : Which of the following defines an open reading frame (ORF)? A- .A sequence of genome in bacteriophage B- The sequence of a complete genome C- A plasmid vector used in genome sequencing D- .A possible gene predicted by DNA sequencing

Last Answer : .A possible gene predicted by DNA sequencing

Which of the following defines an open reading frame (ORF)? A- .A sequence of genome in bacteriophage B- The sequence of a complete genome C- A plasmid vector used in genome sequencing D- .A possible gene predicted by DNA sequencing

Description : Which of the following defines an open reading frame (ORF)? A- .A sequence of genome in bacteriophage B- The sequence of a complete genome C- A plasmid vector used in genome sequencing D- .A possible gene predicted by DNA sequencing

Last Answer : .A possible gene predicted by DNA sequencing

What is the term used for a segment of DNA with one or more genes in the centre and the twoends carrying inverte d repeat sequences of nucleotides? A- Plasmid B- Transposon C- Insertion sequence D- None of these

Description : What is the term used for a segment of DNA with one or more genes in the centre and the twoends carrying inverte d repeat sequences of nucleotides? A- Plasmid B- Transposon C- Insertion sequence D- None of these

Last Answer : Transposon

In the extracellular medium, DNA-degrading enzymes would likely be to prevent transfer of DNA by A- conjugal transfer by a self-transmissible plasmid B- generalized phage transduction C- natural transformation D- none of the above

Description : In the extracellular medium, DNA-degrading enzymes would likely be to prevent transfer of DNA by A- conjugal transfer by a self-transmissible plasmid B- generalized phage transduction C- natural transformation D- none of the above

Last Answer : natural transformation

The main difference between a self-transmissible and a mobilizableplasmidis that the selftransmissible plasmid A- transfers both strands of the plasmid DNA B- carries genes encoding the mating apparatus C- transfers antibiotic resistance genes D- usually has a transposon inserted into it

Description : The main difference between a self-transmissible and a mobilizableplasmidis that the selftransmissible plasmid A- transfers both strands of the plasmid DNA B- carries genes encoding the mating apparatus C- transfers antibiotic resistance genes D- usually has a transposon inserted into it

Last Answer : carries genes encoding the mating apparatus

Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes ? (1) Retrovirus (2) Ti plasmid (3)  phage (4) pBR 322

Description : Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes ? (1) Retrovirus (2) Ti plasmid (3)  phage (4) pBR 322

Last Answer : (1) Retrovirus

Lysis of foreign cell is initiated by antibodies of:

Description : Lysis of foreign cell is initiated by antibodies of:

Last Answer : Lysis of foreign cell is initiated by antibodies of: A. IgM B. IgA C. IgE D. IgM and IgG

Euglobulin lysis time?

Description : Euglobulin lysis time?

Last Answer : DefinitionEuglobulin lysis time (ELT) is a blood test that looks at how fast clots breakdown in the blood.Alternative NamesEuglobulin clot lysis; Fibrinolysis/euglobulin lysis; ELTHow the test is ... stinging sensation. Afterward, there may be some throbbing.Why the test is performedThis is o

An enzyme which brings about lysis of bacterial cell wall is (A) Amylase (B) Lysozyme (C) Trypsin (D) Lipase

Description : An enzyme which brings about lysis of bacterial cell wall is (A) Amylase (B) Lysozyme (C) Trypsin (D) Lipase

Last Answer : Answer : B

Thrombolytic therapy has become a useful adjunct in the management of peripheral arterial occlusion. In this setting, direct intraarterial administration rather than intravenous has been advocated to decrease the risk of systemic bleeding. Which of the following true statement(s) concerning the use of thrombolytic agents for arterial occlusion is/are true? a. A standard technique involves infusing high-dose urokinase, 4000 units per minute for 1–2 hours, directly into the clot by a catheter embedded in the thrombus b. If progress is made, further fibrinolytic therapy is given at 1000 to 2000 units per minute until clot lysis has occurred c. The usual infusion time by the above-stated technique is usually in excess of 24 hours d. Successful clot lysis occurs more frequently in arterial graf

Description : Thrombolytic therapy has become a useful adjunct in the management of peripheral arterial occlusion. In this setting, direct intraarterial administration rather than intravenous has been advocated ... may be indicated for situations where complete clot evacuation cannot be accomplished surgically

Last Answer : Answer: a, b, e The most popular method for intraarterial thrombolytic therapy for arterial occlusion involves passing a guidewire through the thrombus with arteriographic guidance and ... acute arterial occlusion) or when distal vasculative is occluded and precludes appropriate inflow patency

Cyanide poisoning causes death in seconds because - (1) It denatures enzymes of the heart/muscle (2) It breaks the electron transport chain (3) It causes Lysis of red blood cells (4) It causes cardiac arrest

Description : Cyanide poisoning causes death in seconds because - (1) It denatures enzymes of the heart/muscle (2) It breaks the electron transport chain (3) It causes Lysis of red blood cells (4) It causes cardiac arrest

Last Answer : (4) It causes cardiac arrest Explanation: Cyanide poisoning occurs on exposure to a com-pound that produces cyanide ions when dissolved in water. The cyanide ion halts cellular respiration by inhibiting ... coma with seizures, apnea, and cardiac arrest, with death following in a matter of seconds.

.Viruses that infect bacteria multiply and cause their lysis, are called (a) lysozymes (b) lipolytic (c) lytic (d) lysogenic

Description : .Viruses that infect bacteria multiply and cause their lysis, are called (a) lysozymes (b) lipolytic (c) lytic (d) lysogenic

Last Answer : (c) lytic

A common trait among Herpes viruses is _____. a. Lysogeny b. Lysis c. Laxity d. Latency

Description : A common trait among Herpes viruses is _____. a. Lysogeny b. Lysis c. Laxity d. Latency

Last Answer : d. Latency

When a viral nucleic acid becomes part of the host cell chromosome it is called _____. a. lysis b. lysogeny c. maturation d. latent

Description : When a viral nucleic acid becomes part of the host cell chromosome it is called _____. a. lysis b. lysogeny c. maturation d. latent

Last Answer : b. lysogeny

Preservation methods such as salting result in the microbial cells. a. Loss Of Salt From b. Gain Of Water Into c. Loss Of Water From d. Lysis Of

Description : Preservation methods such as salting result in the microbial cells. a. Loss Of Salt From b. Gain Of Water Into c. Loss Of Water From d. Lysis Of

Last Answer : c. Loss Of Water From

Area of lysis on a bacterial lawn culture produced by a phage is known as A.pock B.plaque C.pox D.all of these

Description : Area of lysis on a bacterial lawn culture produced by a phage is known as A.pock B.plaque C.pox D.all of these

Last Answer : B.plaque

Area of lysis on a bacterial lawn culture produced by a phage is known as A.pock B.plaque C.pox D.all of these

Description : Area of lysis on a bacterial lawn culture produced by a phage is known as A.pock B.plaque C.pox D.all of these

Last Answer : .plaque

Cyanide poisoning causes death in seconds because (1) It denatures enzymes of the heart/muscle (2) It breaks the electron transport chain (3) It causes Lysis of red blood cells. (4) It causes cardiac arrest

Description : Cyanide poisoning causes death in seconds because (1) It denatures enzymes of the heart/muscle (2) It breaks the electron transport chain (3) It causes Lysis of red blood cells. (4) It causes cardiac arrest

Last Answer : It causes cardiac arrest

Read the following four statements (A-D) about certain mistakes in two of them. (A) The first transgenic buffalo, Rosie produced milk which was human alpha-lactalbumin enriched. (B) Restriction enzymes are used in isolation of DNA from other macromolecules. (C) Downstream processing is one of the steps of rDNA technology. (D) Disarmed pathogen vectors are also used in transfer of rDNA into the host. Which of the two statements have mistakes? (a) B and C (b) C and D (c) A and C (d) A and B

Description : Read the following four statements (A-D) about certain mistakes in two of them. (A) The first transgenic buffalo, Rosie produced milk which was human alpha-lactalbumin enriched. (B) Restriction enzymes are used in isolation of DNA ... mistakes? (a) B and C (b) C and D (c) A and C (d) A and B

Last Answer : (d) A and B

During the process of isolation of DNA, chilled ethanol is added to (a) precipitate DNA (b) break open the cell to release DNA (c) facilitate action of restriction enzymes (d) remove proteins such as histones.

Description : During the process of isolation of DNA, chilled ethanol is added to (a) precipitate DNA (b) break open the cell to release DNA (c) facilitate action of restriction enzymes (d) remove proteins such as histones.

Last Answer : a) precipitate DNA

Gel electrophoresis is used for (a) construction of recombinant DNA by joining with cloning vectors (b) isolation of DNA molecules (c) cutting of DNA into fragments (d) separation of DNA fragments according to their size.

Description : Gel electrophoresis is used for (a) construction of recombinant DNA by joining with cloning vectors (b) isolation of DNA molecules (c) cutting of DNA into fragments (d) separation of DNA fragments according to their size.

Last Answer : (d) separation of DNA fragments according to their size.

The 1992 Nobel Prize for medicine was awarded to Edmond H. Fischer and Edwin J. Krebs for their work concerning (a) reversible protein phosphorylation as a biological regulation mechanism (b) isolation of the gene for a human disease (c) human genome project (d) drug designing involving inhibition of DNA synthesis of the pathogen.

Description : The 1992 Nobel Prize for medicine was awarded to Edmond H. Fischer and Edwin J. Krebs for their work concerning (a) reversible protein phosphorylation as a biological regulation mechanism (b) ... (c) human genome project (d) drug designing involving inhibition of DNA synthesis of the pathogen.

Last Answer : (a) reversible protein phosphorylation as a biological regulation mechanism

Electrophoresis is mainly used for: (a) Differentiate the biological sample (b) To perform the human specific presumptive tests (c) DNA isolation from biological material (d) Separates the molecules

Description : Electrophoresis is mainly used for: (a) Differentiate the biological sample (b) To perform the human specific presumptive tests (c) DNA isolation from biological material (d) Separates the molecules

Last Answer : Ans: (d). Separate the molecules

Is learning words in isolation a poor method for aiding memory?

Description : Is learning words in isolation a poor method for aiding memory?

Last Answer : This is true. In order to understand the words, you need tolearn them in context with other words so that they make moresense.

ELISA is used to detect viruses where the key reagent is (a) alkaline phosphatase (b) catalase (c) DNA probe (d) RNase.

Description : ELISA is used to detect viruses where the key reagent is (a) alkaline phosphatase (b) catalase (c) DNA probe (d) RNase.

Last Answer : (a) alkaline phosphatase

Which of the following cut the DNA from specific places? (a) E.coli restriction endonuclease I (b) Ligase (c) Exonuclease (d) Alkaline phosphate

Description : Which of the following cut the DNA from specific places? (a) E.coli restriction endonuclease I (b) Ligase (c) Exonuclease (d) Alkaline phosphate

Last Answer : (a) E.coli restriction endonuclease I

What are the uses of plasmid? -Biology

Description : What are the uses of plasmid? -Biology

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What is plasmid used for? -Biology

Description : What is plasmid used for? -Biology

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Why do bacteria have plasmid? -Biology

Description : Why do bacteria have plasmid? -Biology

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What is the origin of replication in a plasmid? -Biology

Description : What is the origin of replication in a plasmid? -Biology

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Differences between a bacterial chromosome and a plasmid? -Biology

Description : Differences between a bacterial chromosome and a plasmid? -Biology

Last Answer : answer: