Description : 1. Bacteria protect themselves from viruses by fragmenting viral DNA with a. Ligase b. Endonuclease c. Exonuclease d. Gyrase
Last Answer : Ans: Endonuclease.
Description : A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using (a) EcoRI (b) Taq polymerase (c) polymerase III (d) ligase.
Last Answer : (d) ligase.
Description : Recombinant DNA is achieved by cleaving the pro-DNAs by (a) ligase (b) restriction endonuclease (c) primase (d) exonucleases.
Last Answer : (b) restriction endonuclease
Description : Manipulation of DNA in genetic engineering became possible due to the discovery of (a) restriction endonuclease (b) DNA ligase (c) transcriptase (d) primase
Last Answer : (a) restriction endonuclease
Description : A seals sticky ends of recombinant DNA segments. a. DNA Ligase b. restriction endonuclease c. protease d. RNA polymerase
Last Answer : b. restriction endonuclease
Description : Following statements describe the characteristics of the enzyme restriction endonuclease. Identify the incorrect statement. (a) The enzyme recognises a specific palindromic nucleotide sequence in the DNA. (b) ... (d) The enzyme cuts the sugar-phosphate backbone at specific sites on each strand.
Last Answer : (c) The enzyme binds DNA at specific sites and cuts only one of the two strands.
Description : There is a restriction endonuclease called EcoRI. What does “co” part in it stand for? (a) colon (b) coelom (c) coenzyme (d) coli
Last Answer : (d) coli
Description : Restriction endonuclease (a) synthesizes DNA (b) cuts the DNA molecule randomly (c) cuts the DNA molecule at specific sites (d) restricts the synthesis of DNA inside the nucleus.
Last Answer : (c) cuts the DNA molecule at specific sites
Description : Restriction endonucleases are enzymes which (a) make cuts at specific positions within the DNA molecule (b) recognize a specific nucleotide sequence for binding of DNA ligase (c) restrict the action of the enzyme DNA polymerase (d) remove nucleotides from the ends of the DNA molecule
Last Answer : (a) make cuts at specific positions within the DNA molecule
Description : Which of the following enzymes are used to join bits of DNA? (a) Ligase (b) Primase (c) DNA polymerase (d) Endonuclease
Last Answer : a) Ligase
Description : What is the difference between exonuclease and endonuclease? -Biology
Last Answer : answer:
Description : Which one of the following hydrolyses internal phosphodiester bonds in a polynucleotide chain? (a) Lipase (b) Protease (c) Endonuclease (d) Exonuclease
Last Answer : (c) Endonuclease
Last Answer : c) G + C / A + T
Description : In sticky ends produced by restriction endonucleases (A) The 2 strands of DNA are joined to each other (B) The DNA strands stick to the restriction endonuclease (C) The ends of a double stranded fragment are overlapping (D) The ends of a double stranded fragment are non overlapping
Last Answer : Answer : C
Description : Genetic engineering requires enzyme: (A) DNA ase (B) Amylase (C) Lipase (D) Restriction endonuclease
Last Answer : Answer : D
Description : Enzyme which can break and seal the DNA strand a) Topoisomease II (b) Helicase (c) Primase (d) Restriction endonuclease
Last Answer : a) Topoisomease II
Description : DNA ligase of E. coli requires which of the following co-factors? (A) FAD (B) NAD+ (C) NADP+ (D) NADH
Last Answer : Answer : B
Description : Role of restriction enzymes and DNA ligase -Biology
Description : 7. In a recombinant DNA technology a plasmid vector must be cleaved by a) the same enzyme that leaves the donor gene b) modified DNA ligase c) a heated alkaline solution d) four separate enzymes
Last Answer : a) the same enzyme that leaves the donor gene
Description : What determines where a restriction endonuclease cuts? -Biology
Description : Function of restriction endonuclease in bacteria -Biology
Description : What is restriction endonuclease used for? -Biology
Description : Which of the following is a restriction endonuclease? (a) DNase I (b) RNase (c) Hind II (d) Protease
Last Answer : (c) Hind II
Description : Which complementary sequence would NOT be recognized by a restriction endonucLease? a. GAATTC CTTAAG b. AAGCTT TTCGAA c. GTCGAC CAGCTG d. AATTCCTTAAGG
Last Answer : d. AATTCCTTAAGG
Description : Which of the following statement is correct? A- The size and sequence of introns can be deduced from the cDNA sequence B- Restriction endonuclease can cleave ss and dsDNA both C- Restriction ... sequenceof a gene D- Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide
Last Answer : Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide
Description : Which of the following statement is correct? A- The size and sequence of introns can be deduced from the cDNA sequence B- .Restriction endonuclease can cleave ss and dsDNA both C- Restriction ... of a Gene D- Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide
Description : Restriction endonucleases (A) Cut RNA chains at specific locations (B) Excise introns from hnRNA (C) Remove Okazaki fragments (D) Act as defensive enzymes to protect the host bacterial DNA from DNA of foreign organisms
Description : The restriction enzymes are used in genetic engineering, because (a) they can cut DNA at specific base sequence (b) they are nucleases that cut DNA at variable sites (c) they can degrade harmful proteins (d) they can join different DNA fragments.
Last Answer : (a) they can cut DNA at specific base sequence
Description : Genetic engineering is possible, because (a) we can cut DNA at specific sites by endonucleases like DNase I (b) restriction endonucleases purified from bacteria can be used in vitro (c) the phenomenon of transduction in bacteria is well understood (d) we can see DNA by electron microscope
Last Answer : (b) restriction endonucleases purified from bacteria can be used in vitro
Description : he molecular scissors use3d to cut DNA into specific genes of interest are called (a) Exonucleases (b) Restriction endonucleases (c) Ligases (d) Polymerases
Last Answer : (b) Restriction endonucleases
Description : The given figure is the diagrammatic representation of the E. coli vector pBR322. Which one of the given options correctly identifies its certain component(s)? Bam H1 Sal 1 Pvu II rop ... reduced osmotic pressure (c) HindIII, EcoRI - selectable markers (d) ampR, tetR-antibiotic resistance genes
Last Answer : (d) ampR, tetR–antibiotic resistance genes
Description : What is exonuclease activity of DNA polymerase? -Biology
Description : 3’→5’ Exonuclease activity of DNA polymerase I (A) Removes ribonucleotides (B) Adds deoxyribonucleotides (C) Corrects errors in replication (D) Hydrolyses DNA into mononucleotides
Description : DNA polymerase I possesses (A) Polymerase activity (B) 3’→5’ Exonuclease activity (C) 5’→3’ Exonuclease activity (D) All of these
Description : DNA polymerase III holoenzyme possesses (A) Polymerase activity (B) 3’→5’ Exonuclease activity (C) 5’→3’ Exonuclease and polymerase activities (D) 3’→5’ Exonuclease and polymerase activities
Description : Assetion: Restriction enzymes cut the strand 27 of DNA to produce sticky ends Reason : Stickiness of the ends faciliates the action of the enzyme DNA
Last Answer : Assetion: Restriction enzymes cut the strand 27 of DNA to produce sticky ends Reason : Stickiness of ... D. If both assertion and reason are false
Description : A foreign DNA and plasmid cut by the same restriction endonculease can be joined to form a recombinant plasmid using
Last Answer : A foreign DNA and plasmid cut by the same restriction endonculease can be joined to form a recombinant ... B. Ligase C. Eco RI D. Taq polymerase
Description : Restriction endonucleases recognize and cut a certain sequence of (A) Single stranded DNA (B) Double stranded DNA (C) RNA (D) Protein
Description : Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme? (a) (b) (c) (d)
Last Answer : (c)
Description : Identify the wrong statement with regard to restriction enzymes. (a) Each restriction enzyme functions by inspecting the length of a DNA sequence. (b) They cut the strand of DNA at palindromic sites. (c) They are useful in genetic engineering. (d) Sticky ends can be joined by using DNA ligases.
Last Answer : d) Sticky ends can be joined by using DNA ligases.
Description : 6. Which of the following DNA sequences would a Restriction enzyme recognize and cut? a. ATGCAC TACGTG b. GATATC CTATAG c. TAGATA ATCTAT d. AATATA TTATAT
Last Answer : b. GATATC CTATAG
Description : The cutting of DNA at specific locations became possible with the discovery of (a) selectable markers (b) ligases (c) restriction enzymes (d) probes.
Last Answer : (c) restriction enzymes
Description : Which of the following are examples of repair mechanisms in cells? a. mismatch repair b. excision repair c. DNA polymerase d. DNA ligase e. all are important in fixing mutations
Last Answer : e. all are important in fixing mutations
Description : Why is ligase needed to make recombinant DNA? -Biology
Description : What is the role of the DNA ligase in DNA replication?
Last Answer : It joins Okazaki fragments
Description : $ The enzymes which joins short pieces DNA in the laggins strand is called ligase. ! Lagging strand and leading strand are formed due to replication f
Last Answer : $ The enzymes which joins short pieces DNA in the laggins strand is called ligase. ! Lagging strand and ... wrong D. If both As and R are wrong.
Description : What is the role of the DNA ligase in DNA replication apex?
Last Answer : It ties the chunks of DNA together.
Description : In the biosynthesis of c-DNA, the joining enzyme ligase requires (A) GTP (B) ATP (C) CTP (D) UTP