Explain the principle and application of ELISA? -Biology

Explain the principle and application of ELISA? -Biology
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What is the basic principle of ELISA ?

Description : What is the basic principle of ELISA ?

Last Answer : The basic principle is an enzyme conjugated to n antibody reacts with a colorless substrate to generate a colored product.

Which disease can be detected using ELISA? -Biology

Description : Which disease can be detected using ELISA? -Biology

Last Answer : answer:

What is the full form of ELISA? -Biology

Description : What is the full form of ELISA? -Biology

Last Answer : answer:

What is the full form of ELISA ?

Last Answer : : ELISA - Enzyme Linked Immuno Sorbent Assay

After ELISA positive test, AIDS is confirmed through

Description : After ELISA positive test, AIDS is confirmed through

Last Answer : After ELISA positive test, AIDS is confirmed through A. Western blot analysis B. Dot blot ... C. Southern blot technique D. Northern blot technique

In what way does Elisa feelings and actions toward the stranger change during her interaction with him?

Description : In what way does Elisa feelings and actions toward the stranger change during her interaction with him?

Last Answer : the answer is c

In the passage Elisa tells the traveling repairman that she doesn't have anything for him to fix. best describes the tone of the traveling repairman's response to Elisa?

Description : In the passage Elisa tells the traveling repairman that she doesn't have anything for him to fix. best describes the tone of the traveling repairman's response to Elisa?

Last Answer : Hhhh

How does the stranger show that he is more aggressive than Henry when interacting with elisa at the garden?

Description : How does the stranger show that he is more aggressive than Henry when interacting with elisa at the garden?

Last Answer : He leans over the fence and eventually enters Elisa's garden while henry respects her space and remains outside

How does the stranger show that he is more aggressive than Henry when interacting with elisa at her graden?

Description : How does the stranger show that he is more aggressive than Henry when interacting with elisa at her graden?

Last Answer : Need answer

What does this description from the beginning of the Chrysanthemums foreshadow about Elisa Feelings?

Description : What does this description from the beginning of the Chrysanthemums foreshadow about Elisa Feelings?

Last Answer : The setting feels isolated and closed in. (Answer for apex)

ELISA/Western blot tests for HIV?

Description : ELISA/Western blot tests for HIV?

Last Answer : DefinitionHIV ELISA/Western blot is a set of blood tests used to diagnose chronic infection with human immunodeficiency virus (HIV).Alternative NamesHIV testingHow the test is performedBlood is typically drawn from ... , and the puncture site is covered to stop any bleeding.In infants or young chi

ELISA?

Description : ELISA?

Last Answer : DefinitionELISA stands for enzyme-linked immunoassay. It is a commonly used laboratory test to detect antibodies in the blood.Alternative NamesEnzyme-linked immunoassay; EIAHow the test is performedBlood is typically ... is removed, and the puncture site is covered to stop any bleeding.In infants

ELISA test is related to

Description : ELISA test is related to

Last Answer : AIDS

The following statement(s) is/are true concerning HIV infection. a. Initial screening with ELISA is highly sensitive but can be associated with a false positive rate of 25% b. Treatment with azidothymidine (AZD) appears to prolong survival when administered early in the disease c. Predisposition to infection in HIV infection is primarily due to reduction in the number of helper T cells d. Common infections in patients with AIDS are Pneumocystis, carinii pneumonia, CMV pneumonitis, Cryptococcus meningitis, and disseminated infection due to atypical mycobacteria

Description : The following statement(s) is/are true concerning HIV infection. a. Initial screening with ELISA is highly sensitive but can be associated with a false positive rate of 25% b. ... , carinii pneumonia, CMV pneumonitis, Cryptococcus meningitis, and disseminated infection due to atypical mycobacteria

Last Answer : Answer: b, c, d Acquired immunodeficiency syndrome (AIDS) is a syndrome caused by the human retrovirus (HIV-1) that infects T lymphocytes and causes severe immunosuppression. Individuals who ... to prolong survival when administered early in the course of disease and is considered routine therapy

The following statement(s) is/are true concerning newer detection methods of systemic infection. a. Enzyme-linked immunosorbent assay (ELISA) is a rapid immunologic assay used for both antigen and antibody detection b. Southern, northern, and western immunoblot techniques are used to detect DNA, RNA, or proteins, respectively c. Polymerase chain reaction (PCR) is a sensitive assay used to detect small amounts of microbial DNA, thus detecting infection at its early stages d. Infectious agents currently detected using advanced molecular techniques include cytomegalovirus (CMV) and human immunodeficiency virus (HIV)

Description : The following statement(s) is/are true concerning newer detection methods of systemic infection. a. Enzyme-linked immunosorbent assay (ELISA) is a rapid immunologic assay used for ... detected using advanced molecular techniques include cytomegalovirus (CMV) and human immunodeficiency virus (HIV)

Last Answer : Answer: a, b, c, d Although the classic detection of infection based on clinical signs of infection and bacterial culture remain the most common clinical tools, increasing ... including CMV and HIV. Furthermore, preliminary investigations into possible detection of fungal pathogens are underway

Which one of the following statements is false ? Allergic asthma is characterized by: a) Response to cyclosporin therapy in severe disease. b) Is characterised by reversible airways obstruction. c) T2 cells are found in bronchoalveolar lavage fluid. d) Affects 5% of the population. e) Is diagnosed routinely by use of ELISA

Description : Which one of the following statements is false ? Allergic asthma is characterized by: a) Response to cyclosporin therapy in severe disease. b) Is characterised by reversible airways obstruction. c) T2 ... fluid. d) Affects 5% of the population. e) Is diagnosed routinely by use of ELISA

Last Answer : Ans: E

What is the full form of ELISA ?

Description : What is the full form of ELISA ?

Last Answer : Enzyme Linked Immuno Sorbant Assay.

Name two enzymes that have been employed for ELISA ?

Description : Name two enzymes that have been employed for ELISA ?

Last Answer : Alkaline, phosphatase, horseradish, preoxidase Para nitro phenyl phosphatase

What is the significance of ELISA ?

Description : What is the significance of ELISA ?

Last Answer : It is used for the detection and for identification of either antigen or antibody.

In how many ways ELISA can be carried out ?

Description : In how many ways ELISA can be carried out ?

Last Answer : It can be carried out in three ways. Indirect ELISA Sand witch ELISA Competitive ELISA

What is the significance of indirect ELISA ?

Description : What is the significance of indirect ELISA ?

Last Answer : It is used for the detection of the presence of serum antibodies against immuno deficiency virus (HIV, the causative agent of AIDS).

'ELISA' test is employed to diagnose - (1) Polio virus (2) AIDS antibodies (3) Tuberculosis bacterium (4) Cancer

Description : 'ELISA' test is employed to diagnose - (1) Polio virus (2) AIDS antibodies (3) Tuberculosis bacterium (4) Cancer

Last Answer : (3) Tuberculosis bacterium Explanation: ELISA is an abbreviation for "enzymelinked immunosorbent assay." An ELISA test uses components of the immune system and chemicals to detect immune responses In the body (for example, to infectious microbes).

What are the enzymes commonly used in ELISA technique.

Description : What are the enzymes commonly used in ELISA technique. 

Last Answer : Alkaline phosphatase (ALP) and horse radish peroxidase (HRP).

What are the disadvantages of RIA, when compared to ELISA?

Description : What are the disadvantages of RIA, when compared to ELISA?

Last Answer : 1. Since radio-isotopes are used, only approved laboratories could take up the assay. 2. The shelf life of the reagent is short.

Which Vitamin is used for ELISA test (AIDS)?

Description : Which Vitamin is used for ELISA test  (AIDS)?

Last Answer : Vitamin B7 (Biotin)

Which of the followings could be an immunodiagnostic test? B A. PCR B. ELISA C. Liver function test D. Biopsy

Description : Which of the followings could be an immunodiagnostic test? B A. PCR B. ELISA C. Liver function test D. Biopsy 

Last Answer : ELISA

Following is the most sensitive test to detect the DNA. C A. ELISA B. Magnetic Resonance Imaging C. PCR D. CT Scanning

Description : Following is the most sensitive test to detect the DNA. C A. ELISA B. Magnetic Resonance Imaging C. PCR D. CT Scanning

Last Answer : PCR

Which of the followings could be an immunodiagnostic test? B A. PCR B. ELISA C. Liver function test D. Biops

Description : Which of the followings could be an immunodiagnostic test? B A. PCR B. ELISA C. Liver function test D. Biops

Last Answer : ELISA

ELISA is used to detect viruses where the key reagent is (a) alkaline phosphatase (b) catalase (c) DNA probe (d) RNase.

Description : ELISA is used to detect viruses where the key reagent is (a) alkaline phosphatase (b) catalase (c) DNA probe (d) RNase.

Last Answer : (a) alkaline phosphatase

In an ELISA, the primary antibody represents a. The patient‘s serum. b. The antibody recognizing the secondary antibody.c. The enzyme-linked (labeled) antibody. d. The antibodies having been washed away.

Description : In an ELISA, the primary antibody represents a. The patient‘s serum. b. The antibody recognizing the secondary antibody. c. The enzyme-linked (labeled) antibody. d. The antibodies having been washed away.

Last Answer : b. The antibody recognizing the secondary antibody.

What serologicaL test requires sheep red blood cells and a preparation of antibodies that recognizes the sheep red blood ceLls? a. ELISA b. Radioimmunoassay c. Immunodiffusion d. Complement fixation test

Description : What serologicaL test requires sheep red blood cells and a preparation of antibodies that recognizes the sheep red blood ceLls? a. ELISA b. Radioimmunoassay c. Immunodiffusion d. Complement fixation test

Last Answer : d. Complement fixation test

_______ is a serological reaction that produces Little or no visible evidence of a reaction. a. Precipitation b. ELISA c. NeutraLization d. Agglutination

Description : _______ is a serological reaction that produces Little or no visible evidence of a reaction. a. Precipitation b. ELISA c. NeutraLization d. Agglutination

Last Answer : a. Precipitation

Of the following blood tests, which confirms the presence of antibodies to HIV? a) Enzyme-linked immunoabsorbant assay (ELISA) ELISA, as well as Western blot assay, identifies and confirms the presence of antibodies to HIV.

Description : Of the following blood tests, which confirms the presence of antibodies to HIV? a) Enzyme-linked immunoabsorbant assay (ELISA) ELISA, as well as Western blot assay, identifies and confirms the presence ... test. Rather, it is an enzyme that transforms single-stranded RNA into a double-stranded DNA.

Last Answer : a) Enzyme-linked immunoabsorbant assay (ELISA) ELISA, as well as Western blot assay, identifies and confirms the presence of antibodies to HIV.

‘ELISA’ test is employed to diagnose : (1) Polio virus (2) AIDS antibodies (3) Tuberculosis bacterium (4) Cancer

Description : ‘ELISA’ test is employed to diagnose : (1) Polio virus (2) AIDS antibodies (3) Tuberculosis bacterium (4) Cancer

Last Answer : Tuberculosis bacterium

Draw and explain the working principle of turbine type flow meter. Mention its Application area.

Description : Draw and explain the working principle of turbine type flow meter. Mention its Application area.

Last Answer : Working Principle: The flow of liquid past the wheel causes the wheel to rotate at a rate which is proportional to the velocity of the fluid. A voltage pulse is induced in ... Suitable for low viscosity high flow measurements 3) Used in petrochemical industries, in Aerospace applications etc.

The hydraulic brake used in automobiles is a direct application of - (1) Archimedes principle (2) Torricellean law (3) Bernoulli's Theorem (4) Pascal's law

Description : The hydraulic brake used in automobiles is a direct application of - (1) Archimedes principle (2) Torricellean law (3) Bernoulli's Theorem (4) Pascal's law

Last Answer : (4) Pascal's law Explanation: Pascal's law basically states that any pressure applied to a fluid inside a closed system will transmit that pressure equally in all directions throughout the fluid.

The hydraulic brakes used in automobiles is a direct application of : (1) Archimedes' principle (2) Toricellian law (3) Bernoulli's theorem (4) Pascal's law

Description : The hydraulic brakes used in automobiles is a direct application of : (1) Archimedes' principle (2) Toricellian law (3) Bernoulli's theorem (4) Pascal's law

Last Answer : (4) Pascal's law Explanation: Pascal's principle guarantees that the pressure is transmitted equally to all parts of the enclosed fluid system. This gives straightline braking unless there is a fluid leak or ... to the actual brake mechanism, which is usually at or near the wheel of the vehicle.

Which of the following is not likely a threat to independence? a. Acting as an advocate on behalf of an assurance client in litigation or in resolving disputes with third parties. b. Long association of a senior member of the assurance team with the assurance client. c. Threat of replacement over a disagreement with the application of an accounting principle. d. Owning immaterial indirect financial interest in an audit client.

Description : Which of the following is not likely a threat to independence? a. Acting as an advocate on behalf of an assurance client in litigation or in resolving disputes with third parties. b. ... the application of an accounting principle. d. Owning immaterial indirect financial interest in an audit client.

Last Answer : Owning immaterial indirect financial interest in an audit client.

Which of the following is not likely a threat to independence? a. Acting as an advocate on behalf of an assurance client in litigation or in resolving disputes with third parties. b. Long association of a senior member of the assurance team with the assurance client. c. Threat of replacement over a disagreement with the application of an accounting principle. d. Owning immaterial indirect financial interest in an audit client.

Description : Which of the following is not likely a threat to independence? a. Acting as an advocate on behalf of an assurance client in litigation or in resolving disputes with third parties. b. ... the application of an accounting principle. d. Owning immaterial indirect financial interest in an audit client.

Last Answer : Owning immaterial indirect financial interest in an audit client.

Give application of simple distillation. What is the principle of fractional distillation?

Description : Give application of simple distillation. What is the principle of fractional distillation?

Last Answer : Applications of simple distillation:  i. It is used for the preparation of distilled water and water for injection. ii. Preparation of many volatile oils and aromatic water. iii. Purification ... becomes richer with the liquid having the lower boiling point which gets condensed in a condenser. 

Give the principle, working and application of” Fractional distillation.

Description : Give the principle, working and application of” Fractional distillation.

Last Answer : Principle of fractional distillation:  It differs from simple distillation in that Partial condensation of vapour is allowed to occur in a fractionating column through which the vapour must pass before reaching ... , such as alcohol & water, acetone & water, Chloroform & Benzene.

Give principle, method and application of sterilization using UV radiation.

Description : Give principle, method and application of sterilization using UV radiation.

Last Answer : Principle:   Direct sunlight can destroy the microorganism on account of its ultra-violet rays of longer wave length. UV light of shorter wavelength kills or inactivates microorganisms by ... . Sterilization of thermolabile material. Sterilization of surface of working table.

Compare Bellows and diaphragm w.r.to construction, sensitivity, working principle and application.

Description : Compare Bellows and diaphragm w.r.to construction, sensitivity, working principle and application.

Last Answer : Sr. No Points Bellows diaphragm 1 construction 2 sensitivity Less sensitive as compared to diaphragm  More sensitive as compared to diaphragm ... as well as for vacuum, compound and differential pressure applications. II. For corrosive gases and liquids

The hydraulic brakes used in automobiles is a direct application of : (1) Archimedes’ principle (2) Toricellian law (3) Bernoulli’s theorem (4) Pascal’s law

Description : The hydraulic brakes used in automobiles is a direct application of : (1) Archimedes’ principle (2) Toricellian law (3) Bernoulli’s theorem (4) Pascal’s law

Last Answer : Pascal’s law

Compare active transducer with passive transducer based on working principle, example, advantage, application

Description : Compare active transducer with passive transducer based on working principle, example, advantage, application

Last Answer : Compare active transducer with passive transducer based on working principle, example, advantage, application

State principle of operation of piezo-electric transducer. State its application.

Description : State principle of operation of piezo-electric transducer. State its application.

Last Answer : The piezoelectric element used for converting mechanical movement into electric signals. The mechanical deformation generates charges and this charge appears as a voltage across the electrodes.  ... destructive test (NDT) equipments 6. Piezoelectric materials are used in ultrasonic transducers.