The principle for separation of immiscible liquids is carried out by using a separation funnel.The two liquids are put into the funnel and left to sit down to form two layers for a short period of time. The funnel tap is opened and the underlying liquid is allowed to run.Separate funnels operate on the idea that these liquids, along with their solutes, can naturally isolate each other, thereby forming different solution-solute strata.For example, on mixing the non polar solution in a separation funnel with a non-polar solvent, it will separate it into new solvent.