Which method of protein estimation is depen- dent on the intact peptide bond?

Which method of protein estimation is depen-

dent on the intact peptide bond?
by

1 Answer

Answer :

Biuret method.
by

Related questions

The formation of a peptide bond during the elongation step of protein synthesis results in the splitting of how many high energy bonds? (A) 1 (B) 2 (C) 3 (D) 4

Description : The formation of a peptide bond during the elongation step of protein synthesis results in the splitting of how many high energy bonds? (A) 1 (B) 2 (C) 3 (D) 4

Last Answer : Answer : B

Which bond is present in the primary structure of protein? (A) Ester (B) Hydrogen (C) Ionic bond (D) Peptide

Description : Which bond is present in the primary structure of protein? (A) Ester (B) Hydrogen (C) Ionic bond (D) Peptide

Last Answer : Answer : D

A protein reacts with biuret reagent which indicates 2 or more (A) Blood clotting (B) Peptide bond (C) Disulphide bonds (D) Hydrophobic bonds

Description : A protein reacts with biuret reagent which indicates 2 or more (A) Blood clotting (B) Peptide bond (C) Disulphide bonds (D) Hydrophobic bonds

Last Answer : Answer : B

What is the basis of Lowryís method of protein estimation?

Description : What is the basis of Lowryís method of protein estimation?

Last Answer : This is based on the reduction of folin-ciocalteau phenol reagent (phosphomolybdic acid and phos- photungstic acid) by the tyrosine and tryptophan residues of protein.

Assertion `:` Removal of gall bladder does affect the protein digestion. Reason `:` Bile juice break the peptide bond.

Description : Assertion `:` Removal of gall bladder does affect the protein digestion. Reason `:` Bile juice break the peptide bond.

Last Answer : Assertion `:` Removal of gall bladder does affect the protein digestion. Reason `:` Bile juice break ... . D. If both Assertion & Reason are false.

Assertion `:` Pancreatic amylase digest protein to amino acid. Reason `:` Pancreatic amylase breaks the peptide bond of protein.

Description : Assertion `:` Pancreatic amylase digest protein to amino acid. Reason `:` Pancreatic amylase breaks the peptide bond of protein.

Last Answer : Assertion `:` Pancreatic amylase digest protein to amino acid. Reason `:` Pancreatic amylase breaks the ... D. If both Assertion & Reason are false.

Which of the following statement(s) is/are true concerning translation of the mRNA message to protein synthesis? a. An adaptor molecule, tRNA, recognizes specific nucleic acid bases and unites them with specific amino acids b. Covalent attachment of tRNA to amino acids is energy dependent c. The formation of a peptide bond between the growing peptide chain and the free amino acid occurs in the free cytoplasm d. Complete protein synthesis takes hours

Description : Which of the following statement(s) is/are true concerning translation of the mRNA message to protein synthesis? a. An adaptor molecule, tRNA, recognizes specific nucleic acid bases and unites them ... and the free amino acid occurs in the free cytoplasm d. Complete protein synthesis takes hours

Last Answer : Answer: a, b The synthesis of protein involves conversion from a four-letter nucleotide language to one of 20 chemically distinct amino acids. This process is referred to as ... translation and be moving down the mRNA molecules simultaneously, thus increasing the rate of protein synthesis

The covalent bond that is repeatedly present between different amino acid residues in a protein is called (a) p-bond (b) hydrogen bond (c) peptide bond (d) metallic bond

Description : The covalent bond that is repeatedly present between different amino acid residues in a protein is called (a) p-bond (b) hydrogen bond (c) peptide bond (d) metallic bond

Last Answer : Ans:(c)

Chloramphenicol inhibits bacterial protein synthesis by: A. Binding to 30S ribosome and inhibiting attachment of aminoacyl tRNA B. Binding to 50S ribosome and preventing peptide bond formation C. Binding to 50S ribosome and blocking translocation of peptide chain D. Binding to both 30S and 50S ribosome and inducing misreading of mRNA code

Description : Chloramphenicol inhibits bacterial protein synthesis by: A. Binding to 30S ribosome and inhibiting attachment of aminoacyl tRNA B. Binding to 50S ribosome and preventing peptide bond formation C. Binding to ... chain D. Binding to both 30S and 50S ribosome and inducing misreading of mRNA code

Last Answer : B. Binding to 50S ribosome and preventing peptide bond formation

The absorption of intact protein from the gut in the foetal and newborn animals takes place by (A) Pinocytosis (B) Passive diffusion (C) Simple diffusion (D) Active transport

Description : The absorption of intact protein from the gut in the foetal and newborn animals takes place by (A) Pinocytosis (B) Passive diffusion (C) Simple diffusion (D) Active transport

Last Answer : A

After formation of a peptide bond, mRNA is translocated along the ribosome by (A) eEF-1 and GTP (B) eEF-2 and GTP (C) Peptidyl transferase and GTP (D) Peptidyl transferase and ATP

Description : After formation of a peptide bond, mRNA is translocated along the ribosome by (A) eEF-1 and GTP (B) eEF-2 and GTP (C) Peptidyl transferase and GTP (D) Peptidyl transferase and ATP

Last Answer : Answer : B

In denaturation of proteins, the bond which is not broken: (A) Disulphide bond (B) Peptide bond (C) Hydrogen bond (D) Ionic bond

Description : In denaturation of proteins, the bond which is not broken: (A) Disulphide bond (B) Peptide bond (C) Hydrogen bond (D) Ionic bond

Last Answer : Answer : B

The bond in proteins that is not broken under usual conditions of denaturation: (A) Hydrophobic bond (B) Hydrogen bond (C) Disulphide bond (D) Peptide bonds

Description : The bond in proteins that is not broken under usual conditions of denaturation: (A) Hydrophobic bond (B) Hydrogen bond (C) Disulphide bond (D) Peptide bonds

Last Answer : Answer : D

From two amino acids peptide bond formation involves removal of one molecule of (A) Water (B) Ammonia (C) Carbondioxide (D) Carboxylic acid

Description : From two amino acids peptide bond formation involves removal of one molecule of (A) Water (B) Ammonia (C) Carbondioxide (D) Carboxylic acid

Last Answer : Answer : A

The bond in proteins that is not hydrolysed under usual conditions of denaturation: (A) Hydrophobic bond (B) Hydrogen bond (C) Disulphide bond (D) Peptide bonds

Description : The bond in proteins that is not hydrolysed under usual conditions of denaturation: (A) Hydrophobic bond (B) Hydrogen bond (C) Disulphide bond (D) Peptide bonds

Last Answer : Answer : C

Which of the following statement about the peptide bond is true? (A) It is a carbon-carbon bond (B) It has cis hydrogen and oxygen groups (C) It is planar (D) It has rotational freedom

Description : Which of the following statement about the peptide bond is true? (A) It is a carbon-carbon bond (B) It has cis hydrogen and oxygen groups (C) It is planar (D) It has rotational freedom

Last Answer : Answer : B

Ninhydrin with evolution of CO2 forms a blue complex with (A) Peptide bond (B) α-Amino acids (C) Serotonin (D) Histamine

Description : Ninhydrin with evolution of CO2 forms a blue complex with (A) Peptide bond (B) α-Amino acids (C) Serotonin (D) Histamine

Last Answer : Answer : B

What are the salient features of a peptide bond?

Description : What are the salient features of a peptide bond?

Last Answer : The peptide bond is a partial double bond. The C-N bond is ëtransí in nature and there is no free- dom of rotation because of the partial double bond character.

What is a peptide bond?

Description : What is a peptide bond?

Last Answer : Alpha carboxyl group of one amino acid reacts with alpha amino group of another amino acid to form a peptide bond or CO-NH bridge 

Intrinsic factor is chemically a (A) Protein (B) Glycoprotein (C) Mucopolysaccaride (D) Peptide

Description : Intrinsic factor is chemically a (A) Protein (B) Glycoprotein (C) Mucopolysaccaride (D) Peptide

Last Answer : Answer : B

Edman’s reaction can be used to (A) Determine the number of tyrosine residues in a protein (B) Determine the number of aromatic amino acid residues in a protein (C) Determine the amino acid sequence of a protein (D) Hydrolyse the peptide bonds in a protein

Description : Edman’s reaction can be used to (A) Determine the number of tyrosine residues in a protein (B) Determine the number of aromatic amino acid residues in a protein (C) Determine the amino acid sequence of a protein (D) Hydrolyse the peptide bonds in a protein

Last Answer : Answer : C

Primary structure of a protein is formed by (A) Hydrogen bonds (B) Peptide bonds (C) Disulphide bonds (D) All of these

Description : Primary structure of a protein is formed by (A) Hydrogen bonds (B) Peptide bonds (C) Disulphide bonds (D) All of these

Last Answer : Answer : B

The hydrogen bonds between peptide linkages of a protein molecules are interfered by (A) Guanidine (B) Uric acid (C) Oxalic acid (D) Salicylic acid

Description : The hydrogen bonds between peptide linkages of a protein molecules are interfered by (A) Guanidine (B) Uric acid (C) Oxalic acid (D) Salicylic acid

Last Answer : Answer : A

What is the peptide bond that connects amino acids in proteins? -Biology

Description : What is the peptide bond that connects amino acids in proteins? -Biology

Last Answer : answer:

What is lost from amino acids in the formation of a peptide bond? -Biology

Description : What is lost from amino acids in the formation of a peptide bond? -Biology

Last Answer : answer:

What two elements are connected in a peptide bond of 2 amino acids? -Biology

Description : What two elements are connected in a peptide bond of 2 amino acids? -Biology

Last Answer : answer:

Why peptide bond is planar? -Biology

Description : Why peptide bond is planar? -Biology

Last Answer : answer:

What is a peptide bond? -Biology

Description : What is a peptide bond? -Biology

Last Answer : answer:

Which antibiotic inhibits peptide bond formation

Description : Which antibiotic inhibits peptide bond formation

Last Answer : Which antibiotic inhibits peptide bond formation A. Streptomycin B. Tetracycline C. Chloramphenicol D. Neomycin

Peptide bond is a key feature in:

Description : Peptide bond is a key feature in:

Last Answer : Peptide bond is a key feature in: A. polysaccharide B. Proteins C. Nucleotide D. Vitamins

What kind of a reaction forms a peptide bond?

Description : What kind of a reaction forms a peptide bond?

Last Answer : dehydration synthesis

The bond between sugar and nitrogenous base in case of DNA is known as (a) Gycosidic bond (b) Phosphodiester bond (c) H-bond (d) Peptide bond

Description : The bond between sugar and nitrogenous base in case of DNA is known as (a) Gycosidic bond (b) Phosphodiester bond (c) H-bond (d) Peptide bond

Last Answer : (a) Gycosidic bond

Identify the substances having glycosidic bond and peptide bond, respectively in their structure. (a) Chitin, cholesterol (b) Glycerol, trypsin (c) Cellulose, lecithin (d) Inulin, insulin

Description : Identify the substances having glycosidic bond and peptide bond, respectively in their structure. (a) Chitin, cholesterol (b) Glycerol, trypsin (c) Cellulose, lecithin (d) Inulin, insulin

Last Answer : (d) Inulin, insulin

Glycine is a unique amino acid because it (a) has no chiral carbon (b) has a sulfur containing R group (c) cannot form a peptide bond (d) is an essential amino acid

Description : Glycine is a unique amino acid because it (a) has no chiral carbon (b) has a sulfur containing R group (c) cannot form a peptide bond (d) is an essential amino acid

Last Answer : has no chiral carbon

Which binding is inhibited by pactamycin? A- Aminoacyl-tRNA to the A-site of 30S subunit B- Initiator-tRNA to 30S/40S initiation complexes C- Peptidyl t-RNA to the 50S subunit D- Formation of peptide bond in P site

Description : Which binding is inhibited by pactamycin? A- Aminoacyl-tRNA to the A-site of 30S subunit B- Initiator-tRNA to 30S/40S initiation complexes C- Peptidyl t-RNA to the 50S subunit D- Formation of peptide bond in P site

Last Answer : Initiator-tRNA to 30S/40S initiation complexes

The coupling of oxidation and phosphorylation in intact mitochondria: (A) Puromycin (B) Oligomycin (C) Streptomycin (D) Gentamycin

Description : The coupling of oxidation and phosphorylation in intact mitochondria: (A) Puromycin (B) Oligomycin (C) Streptomycin (D) Gentamycin

Last Answer : Answer : B

When all the peptide bonds of a protein have been broken down . The result would be

Description : When all the peptide bonds of a protein have been broken down . The result would be

Last Answer : When all the peptide bonds of a protein have been broken down . The result would be A. Amide B. Oligosaccharide C. Polypeptide D. Amino acid

The most important mechanism by which tetracycline antibiotics exert antiimicrobial action is (a) They chelate Ca2+ ions and alter permeability of bacterial cell membrane (b) They bind to 30S ribosomes and inhibit bacterial protein synthesis (c) They bind to 50S ribosomes and interfere with translocation of the growing peptide chain in the bacteria (d) They interfere with DNA mediated RNA synthesis in bacteria

Description : The most important mechanism by which tetracycline antibiotics exert antiimicrobial action is (a) They chelate Ca2+ ions and alter permeability of bacterial cell membrane (b) They bind to 30S ribosomes ... peptide chain in the bacteria (d) They interfere with DNA mediated RNA synthesis in bacteria

Last Answer : Ans: B

Which of the following events does not occur in rough endoplasmic reticulum? (a) Protein folding (b) Protein glycosylation (c) Cleavage of signal peptide (d) Phospholipid synthesis

Description : Which of the following events does not occur in rough endoplasmic reticulum? (a) Protein folding (b) Protein glycosylation (c) Cleavage of signal peptide (d) Phospholipid synthesis

Last Answer : (d) Phospholipid synthesis

Which of the following is the “quaternary structure” of proteins concerned with? (a) sequence of amino acids in the peptide chain (b) description of the way the peptide chains are arranged with respect to each other (c) location of the disulfide bridges in the peptide chain (d) conformation of the protein backbone

Description : Which of the following is the quaternary structure of proteins concerned with? (a) sequence of amino acids in the peptide chain (b) description of the way the peptide chains are arranged with ... (c) location of the disulfide bridges in the peptide chain (d) conformation of the protein backbone

Last Answer : description of the way the peptide chains are arranged with respect to each other

Which of the following is the first step in the determination of the primary structure of proteins? (a) determining the number and kind of amino acids in the peptide (b) reducing the disulfide bridges in the protein (c) protecting the N-terminal of the peptide (d) protecting the C-terminal of the peptide

Description : Which of the following is the first step in the determination of the primary structure of proteins? (a) determining the number and kind of amino acids in the peptide (b) reducing the disulfide bridges ... (c) protecting the N-terminal of the peptide (d) protecting the C-terminal of the peptide

Last Answer : reducing the disulfide bridges in the protein

The last step in synthesis of peptidoglycan is A- attachment of a peptide to muramic acid B- attaching two amino acids to form a cross-link C- attachment of a portion of peptidoglycan to a membrane lipid D- binding of penicillin to a membrane protein

Description : The last step in synthesis of peptidoglycan is A- attachment of a peptide to muramic acid B- attaching two amino acids to form a cross-link C- attachment of a portion of peptidoglycan to a membrane lipid D- binding of penicillin to a membrane protein

Last Answer : attaching two amino acids to form a cross-link

Select the antibiotic which inhibits bacterial protein synthesis by interfering with translocation of elongating peptide chain from acceptor site back to the peptidyl site of the ribosome so that ribosome does not move along the mRNA and the peptide chain is prematurely terminated: A. Chloramphenicol B. Erythromycin C. Tetracycline D. Streptomycin

Description : Select the antibiotic which inhibits bacterial protein synthesis by interfering with translocation of elongating peptide chain from acceptor site back to the peptidyl site of the ribosome so that ... chain is prematurely terminated: A. Chloramphenicol B. Erythromycin C. Tetracycline D. Streptomycin

Last Answer : B. Erythromycin

The most important mechanism by which tetracycline antibiotics exert antimicrobial action is: A. They chelate Ca2+ ions and alter permeability of bacterial cell membrane B. They bind to 30S ribosomes and inhibit bacterial protein synthesis C. They bind to 50S ribosomes and interfere with translocation of the growing peptide chain in the bacteria D. They interfere with DNA mediated RNA synthesis in bacteria

Description : The most important mechanism by which tetracycline antibiotics exert antimicrobial action is: A. They chelate Ca2+ ions and alter permeability of bacterial cell membrane B. They bind to 30S ribosomes ... peptide chain in the bacteria D. They interfere with DNA mediated RNA synthesis in bacteria

Last Answer : B. They bind to 30S ribosomes and inhibit bacterial protein synthesis

Which of the following events does not occur in rough endoplasmic reticulum ? (1) Protein folding (2) Protein glycosylation (3) Cleavage of signal peptide (4) Phospholipid synthesis

Description : Which of the following events does not occur in rough endoplasmic reticulum ? (1) Protein folding (2) Protein glycosylation (3) Cleavage of signal peptide (4) Phospholipid synthesis

Last Answer : (4) Phospholipid synthesis

Anhydro bonds of protein are called (a) Glycosidic (b) Peptide (c) Easter (d) Diester.

Description : Anhydro bonds of protein are called (a) Glycosidic (b) Peptide (c) Easter (d) Diester.

Last Answer : (b) Peptide

What is the best way to remove a bad dent from the roof of my car?

Description : What is the best way to remove a bad dent from the roof of my car?

Last Answer : Get a heavy rubber suction cup like they sell at some home improvement stores, or try a rubber plumber's helper. Have someone pull on the dent in different places while you bump the edges of the dent ( ... mallet or your fist. It won't remove heavy creases, but it might make it look a little better.

Is the Susan G. Komen for the Cure campaign putting a dent in breast cancer statistics?

Description : Is the Susan G. Komen for the Cure campaign putting a dent in breast cancer statistics?

Last Answer : The president of the foundation makes a half a million dollars per year, according to Charity Navigator. That’s a whole lot of money and that’s why I won’t donate to that organization or go out of my way to purchase products with the pink ribbon.

Who should pay for the car's dent?

Description : Who should pay for the car's dent?

Last Answer : answer:C is responsible for the damage. He should pay. Since he can’t, A gets screwed from helping friends out. As they say, no good deed goes unpunished.

When metal is scratched does a small metal filing occur or a dent?

Description : When metal is scratched does a small metal filing occur or a dent?

Last Answer : Depends on what caused the mar.