How do you separate LDH iso-enzymes in laboratory?

How do you separate LDH iso-enzymes in laboratory?
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1 Answer

Answer :

By cellulose acetate electrophoresis at pH 8.6.
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Related questions

The chemical forces that bind most coenzymes and substrates to enzymes such as LDH are (A) Hydrogen bonds (B) Peptide bonds (C) Coordinate bonds (D) Covalent bonds

Description : The chemical forces that bind most coenzymes and substrates to enzymes such as LDH are (A) Hydrogen bonds (B) Peptide bonds (C) Coordinate bonds (D) Covalent bonds

Last Answer : Answer : D

For alkaline phosphatase, how many iso-enzymes are present?

Description : For alkaline phosphatase, how many iso-enzymes are present?

Last Answer : Six.

What are the origins of the CK iso-enzymes?

Description : What are the origins of the CK iso-enzymes?

Last Answer : Eighty percent of molecules in circulation are MM (CK3) variety of skeletal origin, five percent in circulation are MB (CK2) from heart, one percent from brain (BB or CK1) and fifteen percent CKmt from mitochondria.

What are the iso-enzymes of CK?

Description : What are the iso-enzymes of CK?

Last Answer : CK is a dimer, the subunits are called B for brain and M for muscle. Therefore, three iso-enzymes are possible.

Lactate dehydrogenase has how many iso-enzymes?

Description : Lactate dehydrogenase has how many iso-enzymes?

Last Answer : H4, H3M, H2M2, M3H and M4 varieties, forming five iso-enzymes. All these five forms are seen in all persons.

How to differentiate iso-enzymes.

Description : How to differentiate iso-enzymes.

Last Answer : Electrophoresis, heat stability, km value, inhibitor specificity, and tissue localization.

What are iso-enzymes?

Description : What are iso-enzymes? 

Last Answer : They are physically distinct forms of the same enzyme activity. They have identical catalytic properties, but differ in structure.

Isozymes or iso enzymes are those enzyme which A- have same structural forms B- have different structural forms but identical catalytic properties C- catalyses oxidation reactionsD- none of these

Description : Isozymes or iso enzymes are those enzyme which A- have same structural forms B- have different structural forms but identical catalytic properties C- catalyses oxidation reactions D- none of these

Last Answer : have different structural forms but identical catalytic properties

Isozymes or iso enzymes are those enzyme which A- have same structural forms B- have different structural forms but identical catalytic properties C- catalyses oxidation reactions D- none of these

Description : Isozymes or iso enzymes are those enzyme which A- have same structural forms B- have different structural forms but identical catalytic properties C- catalyses oxidation reactions D- none of these

Last Answer : have different structural forms but identical catalytic properties

Substance which increase the activity of certain enzymes called (a) Pro-enzyme (b) Iso-enzymes (d) Co-enzymes (d) Catalysis

Description : Substance which increase the activity of certain enzymes called (a) Pro-enzyme (b) Iso-enzymes (d) Co-enzymes (d) Catalysis

Last Answer : c

How many different proteins may be present in normal LDH? (A) One (B) Two (C) Three (D) Four

Description : How many different proteins may be present in normal LDH? (A) One (B) Two (C) Three (D) Four

Last Answer : Answer : D

LDH assays are most useful in diagnosing diseases of the (A) Heart (B) Pancreas (C) Brain (D) Kidney

Description : LDH assays are most useful in diagnosing diseases of the (A) Heart (B) Pancreas (C) Brain (D) Kidney

Last Answer : Answer : A

An increase in LDH-5 enzyme is seen in the following except (A) Acute hepatitis (B) Muscular distrophies (C) Breast carcinoma (D) Pulmonary embolism

Description : An increase in LDH-5 enzyme is seen in the following except (A) Acute hepatitis (B) Muscular distrophies (C) Breast carcinoma (D) Pulmonary embolism

Last Answer : Answer : D

In which diseases of the following organs, isoenzymes LDH-1 and LDH-2 will be released in plasma? (A) Kidney, R.B.C and Liver (B) Heart, Kidney and R.B.C (C) Heart, Kidney and Liver (D) Heart, Lungs and Brain

Description : In which diseases of the following organs, isoenzymes LDH-1 and LDH-2 will be released in plasma? (A) Kidney, R.B.C and Liver (B) Heart, Kidney and R.B.C (C) Heart, Kidney and Liver (D) Heart, Lungs and Brain

Last Answer : Answer : B

On the third day following onset of acute myocardial infarction, which enzyme estimation will have the best predictive value? (A) Serum AST (B) Serum CK (C) Serum ALT (D) Serum LDH

Description : On the third day following onset of acute myocardial infarction, which enzyme estimation will have the best predictive value? (A) Serum AST (B) Serum CK (C) Serum ALT (D) Serum LDH

Last Answer : Answer : D

Liver and skeletol measle disorders are characterized by on disk proportionate increase in which of the LDH isoenzyme fraction? (A) LDH-1 (B) LDH-1 and LDH-2 (C) LDH-3 and LDH-4 (D) LDH-2 and LDH-3 (E) LDH-5

Description : Liver and skeletol measle disorders are characterized by on disk proportionate increase in which of the LDH isoenzyme fraction? (A) LDH-1 (B) LDH-1 and LDH-2 (C) LDH-3 and LDH-4 (D) LDH-2 and LDH-3 (E) LDH-5

Last Answer : Answer : C

Which enzyme estimation will be helpful in differentiating the elevated serum ALP found in obstructive jaundice as well as bone disorders? (A) Serum AST (B) Serum ALT (C) Serum LDH (D) Serum γ-GT

Description : Which enzyme estimation will be helpful in differentiating the elevated serum ALP found in obstructive jaundice as well as bone disorders? (A) Serum AST (B) Serum ALT (C) Serum LDH (D) Serum γ-GT

Last Answer : Answer : D

If results of the serum bilirubin, serum ALP, LDH and AST determinations suggest obstructive jaundice, the best confirmatory test would be the estimation of (A) Serum ALT (B) Serum 5’ nucleotidase (C) Serum Pseudo cholinesterase (D) None of these

Description : If results of the serum bilirubin, serum ALP, LDH and AST determinations suggest obstructive jaundice, the best confirmatory test would be the estimation of (A) Serum ALT (B) Serum 5’ nucleotidase (C) Serum Pseudo cholinesterase (D) None of these

Last Answer : Answer : B

Patients with hepatocellular jaundice, as compared to those with purely obstructive jaundice tend to have (A) Lower serum ALP, LDH and AST activity (B) Lower serum ALP, Higher LDH and AST activity (C) Higher serum ALP, LDH and AST activity (D) Higher serum ALP, Lower LDH and AST activity

Description : Patients with hepatocellular jaundice, as compared to those with purely obstructive jaundice tend to have (A) Lower serum ALP, LDH and AST activity (B) Lower serum ALP, Higher LDH and AST activity (C) Higher serum ALP, LDH and AST activity (D) Higher serum ALP, Lower LDH and AST activity

Last Answer : Answer : B

The slow moving fraction of LDH is typically increased in pancreas with (A) Cerebrovascular accidents (B) Acute myocardial infarction (C) Acute pancreatitis (D) Acute viral hepatits

Description : The slow moving fraction of LDH is typically increased in pancreas with (A) Cerebrovascular accidents (B) Acute myocardial infarction (C) Acute pancreatitis (D) Acute viral hepatits

Last Answer : Answer : D

From the following myocardial infarction, the earliest serum enzyme to rise is (A) Creatine Kinase (B) GOT (C) GPT (D) LDH

Description : From the following myocardial infarction, the earliest serum enzyme to rise is (A) Creatine Kinase (B) GOT (C) GPT (D) LDH

Last Answer : Answer : A

Which of the following serum enzyme rises in myocardial infarction: (A) Creatine kinase (B) GOT (C) LDH (D) All of these

Description : Which of the following serum enzyme rises in myocardial infarction: (A) Creatine kinase (B) GOT (C) LDH (D) All of these

Last Answer : Answer : D

On the third day of onset of acute myocardial infarction the enzyme elevated is (A) Serum AST (B) Serum CK (C) Serum LDH (D) Serum ALT

Description : On the third day of onset of acute myocardial infarction the enzyme elevated is (A) Serum AST (B) Serum CK (C) Serum LDH (D) Serum ALT

Last Answer : Answer : C

In early stages of myocardial ischemia the most sensitive indicator is the measurement of the activity of (A) CPK (B) SGPT (C) SGOT (D) LDH

Description : In early stages of myocardial ischemia the most sensitive indicator is the measurement of the activity of (A) CPK (B) SGPT (C) SGOT (D) LDH

Last Answer : Answer : A

The isoenzymes of LDH (A) Differ only in a single amino acid (B) Differ in catalytic activity (C) Exist in 5 forms depending on M and H monomer contents (D) Occur as monomers

Description : The isoenzymes of LDH (A) Differ only in a single amino acid (B) Differ in catalytic activity (C) Exist in 5 forms depending on M and H monomer contents (D) Occur as monomers

Last Answer : Answer : C

Hepatocellular jaundice as compared to pure obstructive type of jaundice is characterized by (A) Increased serum alkaline phosphate, LDH and ALT (B) Decreased serum alkaline phosphatase, LDH and ALT (C) Increased serum alkaline phosphatase and decreased levels of LDH and ALT (D) Decreased serum alkaline phosphatase and increased serum LDH and ALT

Description : Hepatocellular jaundice as compared to pure obstructive type of jaundice is characterized by (A) Increased serum alkaline phosphate, LDH and ALT (B) Decreased serum alkaline phosphatase, LDH and ALT (C ... levels of LDH and ALT (D) Decreased serum alkaline phosphatase and increased serum LDH and ALT

Last Answer : Answer : D

What is the advantage of CK estimation over LDH estimation to identify myocardial infarction?

Description : What is the advantage of CK estimation over LDH estimation to identify myocardial infarction?

Last Answer : The CK level starts to rise within three hours of infarction. Therefore, CK estimation is very useful to detect early cases, where ECG changes may be ambiguous. The CK level is not increased in hemolysis or in congestive cardiac failure; and therefore CK has an advantage over LDH.

LDH level in blood is increased in which conditions?

Description : LDH level in blood is increased in which conditions?

Last Answer : Myocardial infarction, hemolytic anemias, muscular dystrophy, carcinomas, leukemias, and any condition which causes necrosis of body cells.

After termination of the synthesis of RNA molecule, the core enzymes separate from the DNA template. The core enzymes then recognize a promoter at which the synthesis of a new RNA molecule commences, with the assistance of (A) Rho (ρ) factor (B) δ factor (C) β factor (D) σ factor

Description : After termination of the synthesis of RNA molecule, the core enzymes separate from the DNA template. The core enzymes then recognize a promoter at which the synthesis of a new RNA molecule commences, with the assistance of (A) Rho (ρ) factor (B) δ factor (C) β factor (D) σ factor

Last Answer : Answer : D

What laboratory procedure you would use to separate and recover the components of a mixture containing BaSO4 Na2CO3 and C10H8?

Description : What laboratory procedure you would use to separate and recover the components of a mixture containing BaSO4 Na2CO3 and C10H8?

Last Answer : Na2CO3 is soluble in water and separated by filtration from a water mixture.Naphtalene is separated by heating when sublimation occur.

How will you separate two immiscible liquids in pharmacy? Draw a libelled sketch of the apparatus used in laboratory.

Description : How will you separate two immiscible liquids in pharmacy? Draw a libelled sketch of the apparatus used in laboratory. 

Last Answer : :Steam Distillation( immiscible liquids): In pair of immiscible liquids , each liquid exerts its own vapour pressure & neither liquid has any appreciable effect on the vapour pressure ... are not decomposed by water , this provides an alternative to distillation under reduced pressure  

What can cause Low Lymphocyte count and elevated LDH?

Description : What can cause Low Lymphocyte count and elevated LDH?

Last Answer : Feel Free to Answer

LDH isoenzymes?

Description : LDH isoenzymes?

Last Answer : DefinitionLDH isoenzymes is a test to check how much lactate dehydrogenase (LDH) is in the blood.Alternative NamesLD; Lactic (lactate) dehydrogenase isoenzymesHow the test is performedBlood is typically drawn ... , and the puncture site is covered to stop any bleeding.In infants or young child

What is the difference between LDH and LDL cholesterol levels?

Description : What is the difference between LDH and LDL cholesterol levels?

Last Answer : LDL cholesterol levels are the calculation of bad levels in your body while HDL is considered to by the levels of good cholesterol in your body. Good cholesterol ratings can be maintained with proper exercise and diet.

patent is presented with SOB. On Xray he has cardiomegaly and rt. pleural effusion. pleural aspiration reveals: protein is <30, LDH <200 IU. dx: CHF. pneumonia. TB hyperproteinemia.

Description : patent is presented with SOB. On Xray he has cardiomegaly and rt. pleural effusion. pleural aspiration reveals: protein is

Last Answer : CHF.

7. In a recombinant DNA technology a plasmid vector must be cleaved by a) the same enzyme that leaves the donor gene b) modified DNA ligase c) a heated alkaline solution d) four separate enzymes

Description : 7. In a recombinant DNA technology a plasmid vector must be cleaved by a) the same enzyme that leaves the donor gene b) modified DNA ligase c) a heated alkaline solution d) four separate enzymes

Last Answer : a) the same enzyme that leaves the donor gene

Glycolipids contain an amino alcohol: (A) Sphingosine (B) Iso-sphingosine (C) Both (A) and (B) (D) None of these

Description : Glycolipids contain an amino alcohol: (A) Sphingosine (B) Iso-sphingosine (C) Both (A) and (B) (D) None of these

Last Answer : Answer : C

In such conditions, iso-enzyme study is helpful or not?

Description : In such conditions, iso-enzyme study is helpful or not?

Last Answer : Yes, tartarate labile iso-enzyme is specific for prostate carcinoma.

What is Regan iso-enzyme?

Description : What is Regan iso-enzyme?

Last Answer : It is the iso-enzyme of alkaline phosphatase, inhibited by phenylalanine. It is of placental origin. It is elevated in about 15% cases of carcinoma of lung, liver and gut and then named as Regan isoenzyme or carcinoplacental iso-enzyme.

When do you estimate total CK and the iso-enzyme?

Description : When do you estimate total CK and the iso-enzyme?

Last Answer : Estimation of total CK is employed in muscular dystrophies and CK-MB iso-enzyme is estimated to identify myocardial infarction.

What is the iso-electric pH of casein?

Description : What is the iso-electric pH of casein?

Last Answer : 4.6.

Give an example of precipitation at iso-electric point.

Description : Give an example of precipitation at iso-electric point.

Last Answer : Casein is precipitated when the solution is brought to iso-electric pH.

What is the iso-electric pH of human albumin?

Description : What is the iso-electric pH of human albumin?

Last Answer : It is 4.7.

What are the characteristic features of iso-elec- tric point?

Description : What are the characteristic features of iso-elec- tric point?

Last Answer : At the pI value, the proteins will not migrate in an electrical field; solubility, buffering capacity and viscosity will be minimum and precipitation will be maximum.

What is iso-electric point of a protein?

Description : What is iso-electric point of a protein? 

Last Answer : At the iso-electric point, the number of anions and cations present on the protein molecule will be equal and the net charge is zero.

What are the characteristic features of iso-elec- tric pH.

Description : What are the characteristic features of iso-elec- tric pH.

Last Answer : At iso-electric point the amino acid will carry no net charge, there is no mobility in electrical field, solubility will be minimum, the tendency for pre- cipitation will be maximum

What is iso-electric point?.

Description : What is iso-electric point?.

Last Answer : The pH at which the molecule carries no net charge is called iso-electric point.

DNA synthesis in laboratory was first achieved by (A) Watson and crick (B) Khorana (C) A.Kornberg (D) Ochoa

Description : DNA synthesis in laboratory was first achieved by (A) Watson and crick (B) Khorana (C) A.Kornberg (D) Ochoa

Last Answer : Answer : C

Laboratory diagnosis of vitamin B12 deficiency can be made by measuring the urinary excretion of (A) Xanthurenic acid (B) Formiminoglutamic acid (C) Methylmalonic acid (D) Homogentisic acid

Description : Laboratory diagnosis of vitamin B12 deficiency can be made by measuring the urinary excretion of (A) Xanthurenic acid (B) Formiminoglutamic acid (C) Methylmalonic acid (D) Homogentisic acid

Last Answer : Answer : C

A 45-year-old solicitor had an onset of severe, crushing, substernal chest pain while attending a football match. He collapsed on his way to the car. Bystander Cardiorespiratory Resuscitation was begun immediately and continued until arrival in Casualty where an endotracheal tube was inserted and ventilation was maintained on 100% oxygen. Investigations revealed: pH 7.13 PaO2 560 mmHg PaCO2 18 mmHg Bicarbonate 5.8 SaO2 98% Based on these laboratory values, which of the following statements best describes his current pathophysiology? 1) He is demonstrating a primary respiratory alkalosis 2) He probably developed a large right to left intracardiac shunt 3) His anion gap is probably normal 4) His oxyhemoglobin curve is shifted to the left 5) His pulmonary artery pressure is probably elevated

Description : A 45-year-old solicitor had an onset of severe, crushing, substernal chest pain while attending a football match. He collapsed on his way to the car. Bystander Cardiorespiratory Resuscitation ... His oxyhemoglobin curve is shifted to the left 5) His pulmonary artery pressure is probably elevated

Last Answer : Answers-5 This young patient with severe central chest pain has probably arrested due to myocardial infarction and arrhythmia. His gases reveal high PO2 following 100% O2 but severe acidosis ... no left to right shunting and high pulmonary pressures would be expected after this arrest scenario.