(3) tissue culture technique Explanation: Plant tissue culture is a collection of techniques used to maintain or grow plant cells, tissues or organs under sterile conditions on a nutrient culture medium of known composition. Plant tissue culture is widely used to produce clones of a plant in a method known as micropropagation. Plant tissue culture relies on the fact that many plant cells have the ability to regenerate a whole plant (totipotency). The culture medium contains a gel (agar) with the proper mixture of nutrients, sugars, vitamins and hormones, which causes the plant part to grow at very rapid rates to produce new plantlets.