What is the advantage of radio-immuno assay?

What is the advantage of radio-immuno assay?
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1 Answer

Answer :

Very small quantities of substances could be accurately measured.
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Related questions

What is radio immuno assay ?

Description : What is radio immuno assay ?

Last Answer : It is a competitive binding assay in which fixed amount of antibody and radiolabelled antigen react in the presence of unlabelled antigen.

In radio immuno assay what is the used to label an antigen ?

Description : In radio immuno assay what is the used to label an antigen ?

Last Answer : In this technique, the antigen is generally labeled with a- emitting isotopes such as I125.

What is importance of radio immuno assay ?

Description : What is importance of radio immuno assay ?

Last Answer : It is the most sensitive technique used for detecting antigen or antibody. This type of reaction is also called as binder ligand assay.

Ames assay is a rapid method for detection of (A) Oncoviruses (B) Retroviuses (C) Chemical carcinogens (D) Typhoid

Description : Ames assay is a rapid method for detection of (A) Oncoviruses (B) Retroviuses (C) Chemical carcinogens (D) Typhoid

Last Answer : Answer : C

Deficiency of vitamin B12 can be diagonised by (A) Carr-Price reaction (B) Ames assay (C) Watson-Schwartz test (D) Schilling test

Description : Deficiency of vitamin B12 can be diagonised by (A) Carr-Price reaction (B) Ames assay (C) Watson-Schwartz test (D) Schilling test

Last Answer : Answer : D

In Ames’ assay, liver homogenate is included in the culture medium because (A) It converts pro-carcinogens into carcinogens (B) Liver can metabolise histidine (C) Salmonella mainly infects liver (D) Liver is very susceptible to cancer

Description : In Ames’ assay, liver homogenate is included in the culture medium because (A) It converts pro-carcinogens into carcinogens (B) Liver can metabolise histidine (C) Salmonella mainly infects liver (D) Liver is very susceptible to cancer

Last Answer : Answer : A

In Ames’ assay, addition of a carcinogen to the culture medium allows S. typhimurium to grow (A) In the presence of histidine (B) In the presence of arginine (C) In the absence of histidine (D) In the absence of arginine

Description : In Ames’ assay, addition of a carcinogen to the culture medium allows S. typhimurium to grow (A) In the presence of histidine (B) In the presence of arginine (C) In the absence of histidine (D) In the absence of arginine

Last Answer : Answer : C

Plasma protein can be separated by (A) Salting out with (NH4)2SO4 (B) Ultracentrifugation (C) Immuno electrophoresis (D) All of these

Description : Plasma protein can be separated by (A) Salting out with (NH4)2SO4 (B) Ultracentrifugation (C) Immuno electrophoresis (D) All of these

Last Answer : Answer : D

The antibodies raised against a pure protein will show only one sharp spike on this technique: (A) Solubility curve (B) Solvent precipitation (C) Molecular weight determination (D) Immuno electrophoresis

Description : The antibodies raised against a pure protein will show only one sharp spike on this technique: (A) Solubility curve (B) Solvent precipitation (C) Molecular weight determination (D) Immuno electrophoresis

Last Answer : Answer : D

A sharp moving boundary is obtained between the pure solvent and solute containing layer in (A) Chromatography (B) Immuno Reactivity (C) Ultra Centrifugation (D) Solubility curve

Description : A sharp moving boundary is obtained between the pure solvent and solute containing layer in (A) Chromatography (B) Immuno Reactivity (C) Ultra Centrifugation (D) Solubility curve

Last Answer : Answer :C

The purity of an isolated protein can be tested by employing various methods. (A) Solubility curve (B) Molecular weight (C) Ultra Centrifugation (D) Immuno Ractivity (E) All of these

Description : The purity of an isolated protein can be tested by employing various methods. (A) Solubility curve (B) Molecular weight (C) Ultra Centrifugation (D) Immuno Ractivity (E) All of these

Last Answer : Answer : C

What is immuno-electrophoresis?

Description : What is immuno-electrophoresis?

Last Answer : Here electrophoretic separation is followed by an antigen-antibody reaction.

How can I perform an assay for the amino acid Taurine?

Description : How can I perform an assay for the amino acid Taurine?

Last Answer : You need to provide more info than that. What are you trying to do? Detect taurine levels?

What is Assay ?

Description : What is Assay ?

Last Answer : Assay means trying to work hard.

Why is phenolpthalein used as the indicator for the assay of tartaric acid?

Description : Why is phenolpthalein used as the indicator for the assay of tartaric acid?

Last Answer : Need answer

Factor XII assay?

Description : Factor XII assay?

Last Answer : DefinitionThe factor XII assay is a blood test to measure the activity of factor XII -- one of the substances involved in blood clotting.Alternative NamesHageman factor assayHow the test is performedBlood is ... the needle is removed, and the puncture site is covered to stop any bleeding.In infant

Factor VIII assay?

Description : Factor VIII assay?

Last Answer : DefinitionThe factor VIII assay is a blood test that measures the activity of factor VIII -- one of the substances involved in blood clotting (coagulation).Alternative NamesPlasma factor VIII antigenHow the ... blood has been collected, the needle is removed, and the puncture site is covered to st

Factor V assay?

Description : Factor V assay?

Last Answer : DefinitionThe factor V assay is a blood test to measure the activity of factor V -- one of the substances involved in blood clotting (coagulation).How the test is performedBlood is typically drawn from a ... , and the puncture site is covered to stop any bleeding.In infants or young children, a shar

Factor II assay?

Description : Factor II assay?

Last Answer : DefinitionThe factor II assay is a test to measure the activity of factor II -- one of the substances involved in blood clotting (coagulation).Alternative NamesProthrombinHow the test is performedBlood is ... needle is removed, and the puncture site is covered to stop any bleeding.In infants o

The following statement(s) is/are true concerning newer detection methods of systemic infection. a. Enzyme-linked immunosorbent assay (ELISA) is a rapid immunologic assay used for both antigen and antibody detection b. Southern, northern, and western immunoblot techniques are used to detect DNA, RNA, or proteins, respectively c. Polymerase chain reaction (PCR) is a sensitive assay used to detect small amounts of microbial DNA, thus detecting infection at its early stages d. Infectious agents currently detected using advanced molecular techniques include cytomegalovirus (CMV) and human immunodeficiency virus (HIV)

Description : The following statement(s) is/are true concerning newer detection methods of systemic infection. a. Enzyme-linked immunosorbent assay (ELISA) is a rapid immunologic assay used for ... detected using advanced molecular techniques include cytomegalovirus (CMV) and human immunodeficiency virus (HIV)

Last Answer : Answer: a, b, c, d Although the classic detection of infection based on clinical signs of infection and bacterial culture remain the most common clinical tools, increasing ... including CMV and HIV. Furthermore, preliminary investigations into possible detection of fungal pathogens are underway

True statements concerning hypoadrenal shock include which of the following? A. Adrenocortical insufficiency may manifest itself as severe shock refractory to volume and pressor therapy. B. The presence of hyperglycemia and hypotension may suggest the diagnosis of shock due to adrenocortical insufficiency. C. Hydrocortisone does not interfere with the serum cortisol assay and should be given to hemodynamically unstable patients suspected of having hypoadrenal shock. D. The rapid adrenocorticotropic hormone (ACTH) stimulation test should be performed to help establish the diagnosis of acute adrenocortical insufficiency.

Description : True statements concerning hypoadrenal shock include which of the following? A. Adrenocortical insufficiency may manifest itself as severe shock refractory to volume and pressor therapy. B. ... test should be performed to help establish the diagnosis of acute adrenocortical insufficiency.

Last Answer : Answer: AD DISCUSSION: Shock due to acute adrenocortical insufficiency is relatively uncommon but must be considered when shock refractory to volume replacement and pressor therapy is present. ... and it is the corticosteroid of choice while the ACTH stimulation test is being performed

The following are useful in the diagnosis of HIV infection: a. polymerase chain reaction b. antibodies by enzyme-liked immunoadsorbent assay c. P24 protein assay d. all above

Description : The following are useful in the diagnosis of HIV infection: a. polymerase chain reaction b. antibodies by enzyme-liked immunoadsorbent assay c. P24 protein assay d. all above

Last Answer : all above

Gray King Assay and Swelling Index of a coal is a measure of its (A) Swelling characteristics (B) Abradability (C) Agglutinating properties (D) Resistance to impact breakage

Description : Gray King Assay and Swelling Index of a coal is a measure of its (A) Swelling characteristics (B) Abradability (C) Agglutinating properties (D) Resistance to impact breakage

Last Answer : (A) Swelling characteristics

A paper disc or small cylinder containing antibiotic is used in the antibiotic sensitivity assay known as the a. Tube dilution method. b. Immunodiffusion method. c. Agar diffusion method. d. Passive detection method.

Description : A paper disc or small cylinder containing antibiotic is used in the antibiotic sensitivity assay known as the a. Tube dilution method. b. Immunodiffusion method. c. Agar diffusion method. d. Passive detection method.

Last Answer : c. Agar diffusion method.

Of the following blood tests, which confirms the presence of antibodies to HIV? a) Enzyme-linked immunoabsorbant assay (ELISA) ELISA, as well as Western blot assay, identifies and confirms the presence of antibodies to HIV.

Description : Of the following blood tests, which confirms the presence of antibodies to HIV? a) Enzyme-linked immunoabsorbant assay (ELISA) ELISA, as well as Western blot assay, identifies and confirms the presence ... test. Rather, it is an enzyme that transforms single-stranded RNA into a double-stranded DNA.

Last Answer : a) Enzyme-linked immunoabsorbant assay (ELISA) ELISA, as well as Western blot assay, identifies and confirms the presence of antibodies to HIV.

Deficiency of vitamin B12 can be diagonised by (A) Carr-Price reaction (B) Ames assay (C) Watson-Schwartz test (D) Schilling test

Description : Deficiency of vitamin B12 can be diagonised by (A) Carr-Price reaction (B) Ames assay (C) Watson-Schwartz test (D) Schilling test

Last Answer : (D) Schilling test

.A test method using organism to deter mine the presence of herbicide effect is a). Herbicide analysis b). Bio assay c). Bio control d). None of these

Description : .A test method using organism to deter mine the presence of herbicide effect is a). Herbicide analysis b). Bio assay c). Bio control d). None of these

Last Answer : b). Bio assay

.Which crop is an indicator plant for the bio assay of Triazines and Aliphatics? a). Sugarbeet b). Sorghum c). Cotton d). Mustard

Description : .Which crop is an indicator plant for the bio assay of Triazines and Aliphatics? a). Sugarbeet b). Sorghum c). Cotton d). Mustard

Last Answer : a). Sugarbeet

Epinasty of cotton is used to measure qualitative assay of a). Atrazine b). 2,4-D c). Diuron d). Alachlor

Description : Epinasty of cotton is used to measure qualitative assay of a). Atrazine b). 2,4-D c). Diuron d). Alachlor

Last Answer : b). 2,4-D

Classical strategy of biological ap proach involves a). Biocontrol agent b). Bioherbicides c). Bio assay d). a & b

Description : Classical strategy of biological ap proach involves a). Biocontrol agent b). Bioherbicides c). Bio assay d). a & b

Last Answer : a). Biocontrol agent

One metal dissolved in another is called w) an alloy x) an assay y) a mineral z) a ceramic

Description : One metal dissolved in another is called w) an alloy x) an assay y) a mineral z) a ceramic 

Last Answer : ANSWER: W -- AN ALLOY 

Human immuno deficiency virus (HIV) has a protein coat and a genetic material which is

Description : Human immuno deficiency virus (HIV) has a protein coat and a genetic material which is

Last Answer : Human immuno deficiency virus (HIV) has a protein coat and a genetic material which is A. Single ... C. Double stranded RNA D. Double stranded DNA

What are immuno diffusion reactions ?

Description : What are immuno diffusion reactions ?

Last Answer : These reactions can be used to determine relative concentrations of antigens and antibodies to compare antigens and to determine the relative purity of an antigen. They are mainly preformed in 1% agarose gels.

Name the two-immuno diffusion techniques ?

Description : Name the two-immuno diffusion techniques ?

Last Answer : • Radial immuno diffusion method and • Double immuno diffusion in two dimensions

What is radial immuno diffusion method ?

Description : What is radial immuno diffusion method ?

Last Answer : It is used to qualitate the antigen. Suitable dilution of antiserum is incorporated in the agar gel. Antigen is added to the wells cut on the surface of the gel. As the antigen diffuses ... of known concentration of antigen, the concentration of antigen in the given sample can be determined.

What is the limitation for radial immuno diffusion method ?

Description : What is the limitation for radial immuno diffusion method ?

Last Answer : This method cannot the antigens present in concentration below 5-10 micro grams/ml.

What is double immuno diffusion method ?

Description : What is double immuno diffusion method ?

Last Answer : In this method, both antigens and antibodies diffuse radically from wells towards each other by establishing a concentration gradient. As equivalence is reached, a visible line of precipitation is observed. ... line of identity with the common epitope and a curved spur with the unique epitope.

What is immuno electrophoresis ?

Description : What is immuno electrophoresis ?

Last Answer : The resolving power of immuno diffusion was greatly enhanced bye immuno electrophoresis. This involves the electrophoretic separation of antigen into its constituent proteins followed by immuno diffusion. ... The formation of precipitin bands can be observed for the individual antigen components.

How is immuno electrophoresis more advance than paper electrophoresis ?

Description : How is immuno electrophoresis more advance than paper electrophoresis ?

Last Answer : In paper electrophoresis, serum proteins can be separated into 5 different bands but the same protein using immuno electrophoresis can be separated into 30 different proteins.

Give some applications of immuno electrophoresis ?

Description : Give some applications of immuno electrophoresis ?

Last Answer : This technique is useful for testing normal and abnormal proteins in serum and urine. It is useful to determine whether a patient produces abnormally a low amount of one or more proteins. It is also used if a patient over produces some serum proteins.

What is counter current immuno electrophoresis ?

Description : What is counter current immuno electrophoresis ?

Last Answer : This technique involves the simultaneous electrophoresis of antigen and antibody in the gel in the opposite direction resulting in precipitation of point where there is optimum concentration of antigen- ... 30 minutes and is 10 times more sensitive than the standard double diffusion technique.

What is immuno fluorescence ?

Description : What is immuno fluorescence ?

Last Answer : Fluorescence is the property of absorbing light ray of particular wavelength and emitting rays in different wavelength. Antigens that are bound to cells or tissue sections can be visualized by tugging the antibody molecule with a fluorescent dye or fluorochrome.

Into how many types is immuno fluorescence is divided ?

Description : Into how many types is immuno fluorescence is divided ?

Last Answer : Immuno fluorescence is divided into 2 types- Direct immuno fluorescence Indirect immuno fluorescence

What is direct immuno fluorescence ?

Description : What is direct immuno fluorescence ?

Last Answer : In this method, the species antibodies are primary antibodies, which are directly conjugated to fluorescent dye.

What is the disadvantage of direct immuno fluorescence ?

Description : What is the disadvantage of direct immuno fluorescence ?

Last Answer : A separate fluorescent conjugate have to be prepared against each antigen to be tested.

What is indirect immuno fluorescence ?

Description : What is indirect immuno fluorescence ?

Last Answer : In a method the primary unlabelled antibody is detected with a number of reagents have been developed for indirect staining. The most common is fluorescence labeled anti isotype antibody such as fluoroscin labeled goat- mouse antibody.

What are the advantages of indirect immuno fluorescence ?

Description : What are the advantages of indirect immuno fluorescence ?

Last Answer : The primary does not need to be conjugated with label. It increases the sensitivity of staining because multiple fluorochrome reagents will bind to each antibody molecule. This method has great flexibility.

What are the uses of indirect immuno fluorescence ?

Description : What are the uses of indirect immuno fluorescence ?

Last Answer : For identifying bacterial species Detecting antigen-antibody complexes in autoimmune diseases Detecting compliment components in tissues. Localizing hormones

What is immuno suppression ?

Description : What is immuno suppression ?

Last Answer : Immuno suppression is particularly given to the patients who are undergoing organ transplantation in the treatment of autoimmunity, graft rejection and in allergy conditions.

Name some of the immuno suppressive agents ?

Description : Name some of the immuno suppressive agents ?

Last Answer : • Cytotoxic agents such as chlorambucil, cyclophosphamide, and azathioprine • Glucocorticoids • Cyclosporine • Antilymphocyte antibodies