Procedure: -
i) Take four jars and put some cotton wool in the bottom of each jar.
ii) Drop five seeds of gram into each jar.
iii) Arrange the conditions for each jar.
JAR-1: -
i) Leave as it is. Therefore seeds lack moisture.
ii) Stopper the jar.
JAR-2: -
i) Add boiled, cooled water to make cotton damp.
ii) Lower a test tube containing a solution of alkaline pyragallol into the tube.
iii) Stopper the jar.
iv) The alkaline pyragallol will absorb all the oxygen in this tube.
v) Hence the seeds lack oxygen.
JAR-3: -
i) Add some water to moisten the wool.
ii) Here seeds are provided with water and oxygen.
iii) Place all three jars in warm place (at room temperature)
JAR-4: -
i) Dampen the cotton wool with water.
ii) Place the jar in a cold place (in refrigerator)
iii) Examine the four jars daily.
Observations: -
i) In Jar-1 germination fails to occur due to lack of water.
ii) In Jar-2 germination fails to occur due to lack of oxygen.
iii) In Jar-3, the process of germination occurs due to the presence of all the
required conditions for germination i.e. oxygen, water and optimum
temperature.
iv) In Jar-4, germination fails to occur due to the very low temperature.
Result: -
Germination of seed requires, water, oxygen and proper temperature.