How is resolution determined in microscope?

How is resolution determined in microscope? 
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1 Answer

Answer :

To determine resolution, one must know the numerical aperture (NA) of the lens system. This denotes the size of the cone of light entering the aperture of the lens. The NA, typically etched into the lens, is multiplied by two. The product is then divided into the wavelength of the visible light, typically 550 nm. (If another form of light such as ultraviolet light were used, the wavelength of that light would be used in the formula.) The result is the resolution of the lens system expressed in nm. Conversion to micrometers is usually the final step.
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