What is immuno-electrophoresis?

What is immuno-electrophoresis?
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Here electrophoretic separation is followed by an antigen-antibody reaction.
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Related questions

Plasma protein can be separated by (A) Salting out with (NH4)2SO4 (B) Ultracentrifugation (C) Immuno electrophoresis (D) All of these

Description : Plasma protein can be separated by (A) Salting out with (NH4)2SO4 (B) Ultracentrifugation (C) Immuno electrophoresis (D) All of these

Last Answer : Answer : D

The antibodies raised against a pure protein will show only one sharp spike on this technique: (A) Solubility curve (B) Solvent precipitation (C) Molecular weight determination (D) Immuno electrophoresis

Description : The antibodies raised against a pure protein will show only one sharp spike on this technique: (A) Solubility curve (B) Solvent precipitation (C) Molecular weight determination (D) Immuno electrophoresis

Last Answer : Answer : D

What is immuno electrophoresis ?

Description : What is immuno electrophoresis ?

Last Answer : The resolving power of immuno diffusion was greatly enhanced bye immuno electrophoresis. This involves the electrophoretic separation of antigen into its constituent proteins followed by immuno diffusion. ... The formation of precipitin bands can be observed for the individual antigen components.

How is immuno electrophoresis more advance than paper electrophoresis ?

Description : How is immuno electrophoresis more advance than paper electrophoresis ?

Last Answer : In paper electrophoresis, serum proteins can be separated into 5 different bands but the same protein using immuno electrophoresis can be separated into 30 different proteins.

Give some applications of immuno electrophoresis ?

Description : Give some applications of immuno electrophoresis ?

Last Answer : This technique is useful for testing normal and abnormal proteins in serum and urine. It is useful to determine whether a patient produces abnormally a low amount of one or more proteins. It is also used if a patient over produces some serum proteins.

What is counter current immuno electrophoresis ?

Description : What is counter current immuno electrophoresis ?

Last Answer : This technique involves the simultaneous electrophoresis of antigen and antibody in the gel in the opposite direction resulting in precipitation of point where there is optimum concentration of antigen- ... 30 minutes and is 10 times more sensitive than the standard double diffusion technique.

A sharp moving boundary is obtained between the pure solvent and solute containing layer in (A) Chromatography (B) Immuno Reactivity (C) Ultra Centrifugation (D) Solubility curve

Description : A sharp moving boundary is obtained between the pure solvent and solute containing layer in (A) Chromatography (B) Immuno Reactivity (C) Ultra Centrifugation (D) Solubility curve

Last Answer : Answer :C

The purity of an isolated protein can be tested by employing various methods. (A) Solubility curve (B) Molecular weight (C) Ultra Centrifugation (D) Immuno Ractivity (E) All of these

Description : The purity of an isolated protein can be tested by employing various methods. (A) Solubility curve (B) Molecular weight (C) Ultra Centrifugation (D) Immuno Ractivity (E) All of these

Last Answer : Answer : C

What is the advantage of radio-immuno assay?

Description : What is the advantage of radio-immuno assay?

Last Answer : Very small quantities of substances could be accurately measured.

Lipoprotiens may be identified more accurately by means of (A) Electrophoresis (B) Ultra centrifugation (C) Centrifugation (D) Immunoelectrophoresis

Description : Lipoprotiens may be identified more accurately by means of (A) Electrophoresis (B) Ultra centrifugation (C) Centrifugation (D) Immunoelectrophoresis

Last Answer : Answer : D

The movement of charged particles towards one of the electrodes under the influence of electrical current is (A) Gel filtration (B) Molecular sieving (C) Gas liquid chromatography (D) Electrophoresis

Description : The movement of charged particles towards one of the electrodes under the influence of electrical current is (A) Gel filtration (B) Molecular sieving (C) Gas liquid chromatography (D) Electrophoresis

Last Answer : Answer : D

Which one of the following statements concerning glutamine is correct? (A) Contains three tetratable groups (B) Is classified as an acidic amino acid (C) Contains an amide group (D) Migrates to the cathode during electrophoresis at pH 7.0

Description : Which one of the following statements concerning glutamine is correct? (A) Contains three tetratable groups (B) Is classified as an acidic amino acid (C) Contains an amide group (D) Migrates to the cathode during electrophoresis at pH 7.0

Last Answer : Answer : C

Which of the following techniques is used to separate proteins based upon differences in their mass? (A) Isoelectric focusing (B) Dialysis (C) SDS-gel Electrophoresis (D) Western blotting

Description : Which of the following techniques is used to separate proteins based upon differences in their mass? (A) Isoelectric focusing (B) Dialysis (C) SDS-gel Electrophoresis (D) Western blotting

Last Answer : Answer : C

Plasma proteins are isolated by (A) Salting out (B) Electrophoresis (C) Flourimetry (D) Both (A) and (B)

Description : Plasma proteins are isolated by (A) Salting out (B) Electrophoresis (C) Flourimetry (D) Both (A) and (B)

Last Answer : Answer : D

Primary structure of proteins can be determined by the use of (A) Electrophoresis (B) Chromatography (C) Ninhydrin (D) Sanger’s reagent

Description : Primary structure of proteins can be determined by the use of (A) Electrophoresis (B) Chromatography (C) Ninhydrin (D) Sanger’s reagent

Last Answer : Answer : D

The technique for purification of proteins that can be made specific for a given protein is (A) Gel filtration chromotography (B) Ion exchange chromatography (C) Electrophoresis (D) Affinity chromatography

Description : The technique for purification of proteins that can be made specific for a given protein is (A) Gel filtration chromotography (B) Ion exchange chromatography (C) Electrophoresis (D) Affinity chromatography

Last Answer : Answer : D

During electrophoresis, what is the least moving lipoprotein?

Description : During electrophoresis, what is the least moving lipoprotein?

Last Answer : Chylomicron (gamma position).

During electrophoresis, what is the fastest moving lipoprotein?

Description : During electrophoresis, what is the fastest moving lipoprotein?

Last Answer : HDL (alpha lipoprotein).

Electrophoresis is commonly employed for what purpose in laboratory?

Description : Electrophoresis is commonly employed for what purpose in laboratory?

Last Answer : For serum electrophoresis and to see abnormalities in serum protein concentrations. 

What are the types of electrophoresis?

Description : What are the types of electrophoresis? 

Last Answer : Horizontal and vertical types.

What are the factors affecting the mobility in electrophoresis?

Description : What are the factors affecting the mobility in electrophoresis?

Last Answer : Net charge on the particles (pI of proteins), mass and shape of the particles, the pH of the medium, strength of electrical field, and properties of the supporting medium.

What is meant by electrophoresis?

Description : What is meant by electrophoresis?

Last Answer : The term refers to the movement of charged particles through an electrolyte when subjected to an electric field.

Human immuno deficiency virus (HIV) has a protein coat and a genetic material which is

Description : Human immuno deficiency virus (HIV) has a protein coat and a genetic material which is

Last Answer : Human immuno deficiency virus (HIV) has a protein coat and a genetic material which is A. Single ... C. Double stranded RNA D. Double stranded DNA

What are immuno diffusion reactions ?

Description : What are immuno diffusion reactions ?

Last Answer : These reactions can be used to determine relative concentrations of antigens and antibodies to compare antigens and to determine the relative purity of an antigen. They are mainly preformed in 1% agarose gels.

Name the two-immuno diffusion techniques ?

Description : Name the two-immuno diffusion techniques ?

Last Answer : • Radial immuno diffusion method and • Double immuno diffusion in two dimensions

What is radial immuno diffusion method ?

Description : What is radial immuno diffusion method ?

Last Answer : It is used to qualitate the antigen. Suitable dilution of antiserum is incorporated in the agar gel. Antigen is added to the wells cut on the surface of the gel. As the antigen diffuses ... of known concentration of antigen, the concentration of antigen in the given sample can be determined.

What is the limitation for radial immuno diffusion method ?

Description : What is the limitation for radial immuno diffusion method ?

Last Answer : This method cannot the antigens present in concentration below 5-10 micro grams/ml.

What is double immuno diffusion method ?

Description : What is double immuno diffusion method ?

Last Answer : In this method, both antigens and antibodies diffuse radically from wells towards each other by establishing a concentration gradient. As equivalence is reached, a visible line of precipitation is observed. ... line of identity with the common epitope and a curved spur with the unique epitope.

What is immuno fluorescence ?

Description : What is immuno fluorescence ?

Last Answer : Fluorescence is the property of absorbing light ray of particular wavelength and emitting rays in different wavelength. Antigens that are bound to cells or tissue sections can be visualized by tugging the antibody molecule with a fluorescent dye or fluorochrome.

Into how many types is immuno fluorescence is divided ?

Description : Into how many types is immuno fluorescence is divided ?

Last Answer : Immuno fluorescence is divided into 2 types- Direct immuno fluorescence Indirect immuno fluorescence

What is direct immuno fluorescence ?

Description : What is direct immuno fluorescence ?

Last Answer : In this method, the species antibodies are primary antibodies, which are directly conjugated to fluorescent dye.

What is the disadvantage of direct immuno fluorescence ?

Description : What is the disadvantage of direct immuno fluorescence ?

Last Answer : A separate fluorescent conjugate have to be prepared against each antigen to be tested.

What is indirect immuno fluorescence ?

Description : What is indirect immuno fluorescence ?

Last Answer : In a method the primary unlabelled antibody is detected with a number of reagents have been developed for indirect staining. The most common is fluorescence labeled anti isotype antibody such as fluoroscin labeled goat- mouse antibody.

What are the advantages of indirect immuno fluorescence ?

Description : What are the advantages of indirect immuno fluorescence ?

Last Answer : The primary does not need to be conjugated with label. It increases the sensitivity of staining because multiple fluorochrome reagents will bind to each antibody molecule. This method has great flexibility.

What are the uses of indirect immuno fluorescence ?

Description : What are the uses of indirect immuno fluorescence ?

Last Answer : For identifying bacterial species Detecting antigen-antibody complexes in autoimmune diseases Detecting compliment components in tissues. Localizing hormones

What is radio immuno assay ?

Description : What is radio immuno assay ?

Last Answer : It is a competitive binding assay in which fixed amount of antibody and radiolabelled antigen react in the presence of unlabelled antigen.

In radio immuno assay what is the used to label an antigen ?

Description : In radio immuno assay what is the used to label an antigen ?

Last Answer : In this technique, the antigen is generally labeled with a- emitting isotopes such as I125.

What is importance of radio immuno assay ?

Description : What is importance of radio immuno assay ?

Last Answer : It is the most sensitive technique used for detecting antigen or antibody. This type of reaction is also called as binder ligand assay.

What is immuno suppression ?

Description : What is immuno suppression ?

Last Answer : Immuno suppression is particularly given to the patients who are undergoing organ transplantation in the treatment of autoimmunity, graft rejection and in allergy conditions.

Name some of the immuno suppressive agents ?

Description : Name some of the immuno suppressive agents ?

Last Answer : • Cytotoxic agents such as chlorambucil, cyclophosphamide, and azathioprine • Glucocorticoids • Cyclosporine • Antilymphocyte antibodies

The expansion for AIDS is - (1) Active Immuno Deficiency Syndrome (2) Acquired Individual Disease Syndrome (3) Acquired Immuno Deficiency Syndrome (4) Acquired Immuno Disease Syndrome

Description : The expansion for AIDS is - (1) Active Immuno Deficiency Syndrome (2) Acquired Individual Disease Syndrome (3) Acquired Immuno Deficiency Syndrome (4) Acquired Immuno Disease Syndrome

Last Answer : (3) Acquired Immuno Deficiency Syndrome Explanation: Human immunodeficiency virus infection I acquired immunodeficiency syndrome (HIV/AIDS) is a disease of the human immune system caused by the human immunodeficiency ... slow the course of the disease and may lead to a near-normal life expectancy.

Acquired Immuno Deficiency Syndrome (AIDS) is caused by – (1) protozoa (2) virus (3) fungus (4) bacteria

Description : Acquired Immuno Deficiency Syndrome (AIDS) is caused by – (1) protozoa (2) virus (3) fungus (4) bacteria

Last Answer : (2) virus Explanation: Human immunodeficiency virus infection/acquired immunodeficiency syndrome (HIV/AIDS) is a disease of the human immune system caused by the human immunodeficiency virus (HIV).

HIV stands for Human Immuno

Description : HIV stands for Human Immuno

Last Answer : deficiency Virus.

AIDS stands for Acquired Immuno

Description : AIDS stands for Acquired Immuno

Last Answer : Deficiency Syndrome.

Match the following list of microbes and their importance. Column I Column II A. Saccharomyces (i) Production of cerevisiae immuno- suppressive agent B. Monascus (ii) Ripening of Swiss purpureus cheese C. Trichoderma (iii)Commercial polysporum production of ethanol D. Propionibacterium (iv) Production of shermanii blood-cholesterol lowering agents (a) A-(iv), B-(ii), C-(i), D-(iii) (b) A-(iii), B-(i), C-(iv), D-(ii) (c) A-(iii), B-(iv), C-(i), D-(ii) (d) A-(iv), B-(iii), C-(ii),D-(i)

Description : Match the following list of microbes and their importance. Column I Column II A. Saccharomyces (i) Production of cerevisiae immuno- suppressive agent B. Monascus (ii) Ripening of Swiss purpureus cheese C. Trichoderma (iii)Commercial ... C-(i), D-(ii) (d) A-(iv), B-(iii), C-(ii),D-(i)

Last Answer : (c) A-(iii), B-(iv), C-(i), D-(ii)

Which of the following is wrongly matched in the given table? Microbe Product Application (a) Streptococcus Streptokinase Removal of clot from blood vessel (b) Clostridium butylicum Lipase Removal of oil stains (c) Trichoderma polysporum Cyclosporin A Immuno- suppressive drug (d) Monascus purpureus Statins Lowering of blood cholesterol

Description : Which of the following is wrongly matched in the given table? Microbe Product Application (a) Streptococcus Streptokinase Removal of clot from blood vessel (b) Clostridium butylicum Lipase ... Cyclosporin A Immuno- suppressive drug (d) Monascus purpureus Statins Lowering of blood cholesterol

Last Answer : (b) Clostridium butylicum Lipase Removal of oil stains

Human immuno deficiency virus (HIV) has a protein coat and a genetic material which is (a) double stranded RNA (b) double stranded DNA (c) single stranded DNA (d) single stranded RNA

Description : Human immuno deficiency virus (HIV) has a protein coat and a genetic material which is (a) double stranded RNA (b) double stranded DNA (c) single stranded DNA (d) single stranded RNA

Last Answer : (d) single stranded RNA.

Which of the following is not a sexually transmitted disease? (a) Trichomoniasis (b) Encephalitis (c) Syphilis (d) Acquired Immuno Deficiency Syndrome

Description : Which of the following is not a sexually transmitted disease? (a) Trichomoniasis (b) Encephalitis (c) Syphilis (d) Acquired Immuno Deficiency Syndrome

Last Answer : (b) Encephalitis

What is not true about tobacco smoking: a. Redox potential favours growth of anaerobic bacteria. b. It is caries immuno-suppressive. c. It is adrenergic. d. Affects neutrophils and chemotactic factors.

Description : What is not true about tobacco smoking: a. Redox potential favours growth of anaerobic bacteria. b. It is caries immuno-suppressive. c. It is adrenergic. d. Affects neutrophils and chemotactic factors.

Last Answer : b. It is caries immuno-suppressive.

What is not true about tobacco smoking, A. Redox potential is reduced resulting in anaerobic bacteria B. It is immuno-suppressive C. It is adrenergic

Description : What is not true about tobacco smoking, A. Redox potential is reduced resulting in anaerobic bacteria B. It is immuno-suppressive C. It is adrenergic

Last Answer : B. It is immuno-suppressive