All of the following may be used as expression vectors except (A) Plasmid (B) Bacteriophage (C) Baculovirus (D) E. coli

All of the following may be used as expression vectors except (A) Plasmid (B) Bacteriophage (C) Baculovirus (D) E. coli
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In genetic engineering, a DNA segment (gene) of interest, is transferred to the host cell through a vector. Consider the following four agents (i-iv) in this regard and select the correct option about which one or more of these can be used as a vector/vectors. (i) Bacterium (ii) Plasmid (iii)Plasmodium (iv) Bacteriophage (a) (i), (ii) and (iv) (b) (i) only (c) (i) and (iii) (d) (ii) and (iv)

Description : In genetic engineering, a DNA segment (gene) of interest, is transferred to the host cell through a vector. Consider the following four agents (i-iv) in this regard and select the correct option about which one or more of these ... ii) and (iv) (b) (i) only (c) (i) and (iii) (d) (ii) and (iv)

Last Answer : (d) (ii) and (iv)

A mutant strain of T4 - Bacteriophage, R-II, fails to lyse the E. coli but when two strains R-IIX and R-IIY are mixed then they lyse the E. coli. What may be the possible reason? (a) Bacteriophage transforms in wild. (b) It is not mutated. (c) Both strains have similar cistrons. (d) Both strains have different cistrons.

Description : A mutant strain of T4 - Bacteriophage, R-II, fails to lyse the E. coli but when two strains R-IIX and R-IIY are mixed then they lyse the E. coli. What may be the possible reason? (a) ... . (b) It is not mutated. (c) Both strains have similar cistrons. (d) Both strains have different cistrons.

Last Answer : (d) Both strains have different cistrons.

Which of the following defines an open reading frame (ORF)? A- .A sequence of genome in bacteriophage B- The sequence of a complete genome C- A plasmid vector used in genome sequencing D- .A possible gene predicted by DNA sequencing

Description : Which of the following defines an open reading frame (ORF)? A- .A sequence of genome in bacteriophage B- The sequence of a complete genome C- A plasmid vector used in genome sequencing D- .A possible gene predicted by DNA sequencing

Last Answer : .A possible gene predicted by DNA sequencing

Which of the following defines an open reading frame (ORF)? A- .A sequence of genome in bacteriophage B- The sequence of a complete genome C- A plasmid vector used in genome sequencing D- .A possible gene predicted by DNA sequencing

Description : Which of the following defines an open reading frame (ORF)? A- .A sequence of genome in bacteriophage B- The sequence of a complete genome C- A plasmid vector used in genome sequencing D- .A possible gene predicted by DNA sequencing

Last Answer : .A possible gene predicted by DNA sequencing

A viral load test detects the A- provirus of HIV in infected cells B- .total amount of virus in the infected host C- number of viruses being released by each infected cell D- .amount of bacteriophage being produced by E. coli cells

Description : A viral load test detects the A- provirus of HIV in infected cells B- .total amount of virus in the infected host C- number of viruses being released by each infected cell D- .amount of bacteriophage being produced by E. coli cells

Last Answer : provirus of HIV in infected cells

Two microbes found to be very useful in genetic engineering are (a) crown gall bacterium and Caenorhabditis elegans (b) Escherichia coli and Agrobacterium tumefaciens (c) Vibrio cholerae and a tailed bacteriophage (d) Diplococcus sp. and Pseudomonas sp

Description : Two microbes found to be very useful in genetic engineering are (a) crown gall bacterium and Caenorhabditis elegans (b) Escherichia coli and Agrobacterium tumefaciens (c) Vibrio cholerae and a tailed bacteriophage (d) Diplococcus sp. and Pseudomonas sp

Last Answer : (b) Escherichia coli and Agrobacterium tumefaciens

Which one of the following vectors is used to replace the defective gene in gene therapy? (a) Adenovirus (b) Cosmid (c) Ri plasmid (d) Ti plasmid

Description : Which one of the following vectors is used to replace the defective gene in gene therapy? (a) Adenovirus (b) Cosmid (c) Ri plasmid (d) Ti plasmid

Last Answer : (a) Adenovirus

Transduction in bacteria is mediated by (a) plasmid vectors (b) phage vectors (c) cosmids (d) F-factors.

Description : Transduction in bacteria is mediated by (a) plasmid vectors (b) phage vectors (c) cosmids (d) F-factors.

Last Answer : (b) phage vectors

: All the following are among the opportunistic organisms that may cause nosocomial disease except: a. Pseudomonas aeruginosa. b. Escherichia coli. c. Staphylococcus aureus. d. Mycobacterium leprae.

Description : : All the following are among the opportunistic organisms that may cause nosocomial disease except: a. Pseudomonas aeruginosa. b. Escherichia coli. c. Staphylococcus aureus. d. Mycobacterium leprae.

Last Answer : d. Mycobacterium leprae.

Difference between plasmid and expression vector. -Biology

Description : Difference between plasmid and expression vector. -Biology

Last Answer : answer:

A gene whose expression helps to identify transformed cell is known as (a) vector (b) plasmid (c) structural gene (d) selectable marker.

Description : A gene whose expression helps to identify transformed cell is known as (a) vector (b) plasmid (c) structural gene (d) selectable marker.

Last Answer : (d) selectable marker.

A gene whose expression helps to identify transformed cell is known as : (1) Vector (2) Plasmid (3) Structural gene (4) Selectable marker

Description : A gene whose expression helps to identify transformed cell is known as : (1) Vector (2) Plasmid (3) Structural gene (4) Selectable marker

Last Answer : 4) Selectable marker

Which of the following may be used as a cloning vector? (A) Prokaryotic plasmid(B) Lambda phage (C) Cosmid (D) All of these

Description : Which of the following may be used as a cloning vector? (A) Prokaryotic plasmid(B) Lambda phage (C) Cosmid (D) All of these

Last Answer : Answer : D

Enhancer elements have all the following features except (A) They increase gene expression through a promoter (B) Each enhancer activates a specific promoter (C) They may be located far away from the promoter (D) They may be upstream or downstream from the promoter

Description : Enhancer elements have all the following features except (A) They increase gene expression through a promoter (B) Each enhancer activates a specific promoter (C) They may be located far away from the promoter (D) They may be upstream or downstream from the promoter

Last Answer : Answer : B

The genine of φ×174 bacteriophage is interesting in that if contains (A) No DNA (B) DNA with uracil (C) Single stranded DNA (D) Triple standard DNA

Description : The genine of φ×174 bacteriophage is interesting in that if contains (A) No DNA (B) DNA with uracil (C) Single stranded DNA (D) Triple standard DNA

Last Answer : Answer : C

How can we derive the expression for cross product of two vectors?

Description : How can we derive the expression for cross product of two vectors?

Last Answer : Here is a video that shows where the term cross comes from. Two vectors in a 3D space when “multiplied” by each other result in a third vector that represents how they “cross” each other.

Difference between cloning vectors and expression vectors -Biology

Description : Difference between cloning vectors and expression vectors -Biology

Last Answer : answer:

Derive an expression for cross product of two vectors and express it in determinant form.

Description : Derive an expression for cross product of two vectors and express it in determinant form.

Last Answer : Derive an expression for cross product of two vectors and express it in determinant form.

Commonly used vectors for human genome sequencing are (a) T - DNA (b) BAC and YAC (c) expression vectors (d) T/A cloning vectors.

Description : Commonly used vectors for human genome sequencing are (a) T - DNA (b) BAC and YAC (c) expression vectors (d) T/A cloning vectors.

Last Answer : (b) BAC and YAC

Which one of the following is used as vector for cloning genes into higher organisms? (a) Baculovirus (b) Salmonella typhimurium (c) Rhizopus nigricans (d) Retrovirus

Description : Which one of the following is used as vector for cloning genes into higher organisms? (a) Baculovirus (b) Salmonella typhimurium (c) Rhizopus nigricans (d) Retrovirus

Last Answer : (d) Retrovirus

. A common biocontrol agent for the control of plant diseases is (a) baculovirus (b) Bacillus thuringiensis (c) Glomus (d) Trichoderma.

Description : . A common biocontrol agent for the control of plant diseases is (a) baculovirus (b) Bacillus thuringiensis (c) Glomus (d) Trichoderma.

Last Answer : (d) Trichoderma.

Select the correct group of biocontrol agents. (a) Nostoc, Azospirillium, Nucleopolyhedrovirus (b) Bacillus thuringiensis, Tobacco mosaic virus, Aphids (c) Trichoderma, Baculovirus, Bacillus thuringiensis (d) Oscillatoria, Rhizobium, Trichoderma

Description : Select the correct group of biocontrol agents. (a) Nostoc, Azospirillium, Nucleopolyhedrovirus (b) Bacillus thuringiensis, Tobacco mosaic virus, Aphids (c) Trichoderma, Baculovirus, Bacillus thuringiensis (d) Oscillatoria, Rhizobium, Trichoderma

Last Answer : (c) Trichoderma, Baculovirus, Bacillus thuringiensis

30. The bioactive moleculae “Statin” is produced by ... b) Trichoderma b) Streptococcus c) Monascus purpureus d) Baculovirus

Description : 30. The bioactive moleculae “Statin” is produced by ... b) Trichoderma b) Streptococcus c) Monascus purpureus d) Baculovirus

Last Answer : c) Monascus purpureus

10. The bioactive moleculae “Statin” is produced by ... a) Trichoderma b) Streptococcus c) Monascus purpureus d) Baculovirus

Description : 10. The bioactive moleculae “Statin” is produced by ... a) Trichoderma b) Streptococcus c) Monascus purpureus d) Baculovirus

Last Answer : c) Monascus purpureus

4. ———– is used as a vector for cloning into higher organisms a. Retrovirus b. Baculovirus c. Salmonella typhimurium d. Rhizopusnigricans

Description : 4. ———– is used as a vector for cloning into higher organisms a. Retrovirus b. Baculovirus c. Salmonella typhimurium d. Rhizopusnigricans

Last Answer : a. Retrovirus

Amoxicillin + Clavulanic acid is active against the following organism except (a) Methicillin resistant Staph. aureus (b) Penicillinase producing Staph. aureus (c) Penicillinase producing n. gonorrhoeae (d) ß-lactamase producing E. coli

Description : Amoxicillin + Clavulanic acid is active against the following organism except (a) Methicillin resistant Staph. aureus (b) Penicillinase producing Staph. aureus (c) Penicillinase producing n. gonorrhoeae (d) ß-lactamase producing E. coli

Last Answer : Ans: A

Amoxicillin + Clavulanic acid is active against the following organisms except: A. Methicillin resistant Staph. aureus B. Penicillinase producing Staph. aureus C. Penicillinase producing N.gonorrhoeae D. β-lactamase producing E.coli

Description : Amoxicillin + Clavulanic acid is active against the following organisms except: A. Methicillin resistant Staph. aureus B. Penicillinase producing Staph. aureus C. Penicillinase producing N.gonorrhoeae D. β-lactamase producing E.coli

Last Answer : A. Methicillin resistant Staph. aureus

Antimicrobial treatment does not alter the course of the following diarrhoeas except: A. Mild enterotoxigenic E.coli diarrhoea B. Campylobacter diarrhoea C. Coeliac disease diarrhoea D. Food poisoning diarrhoea

Description : Antimicrobial treatment does not alter the course of the following diarrhoeas except: A. Mild enterotoxigenic E.coli diarrhoea B. Campylobacter diarrhoea C. Coeliac disease diarrhoea D. Food poisoning diarrhoea

Last Answer : B. Campylobacter diarrhoea

Bacteriophage (pron: back-teer-e-o-faj) are: a) bacteria b) bacteria precursors c) viruses d) agents which cause the production of bacteria

Description : Bacteriophage (pron: back-teer-e-o-faj) are: a) bacteria b) bacteria precursors c) viruses d) agents which cause the production of bacteria

Last Answer : ANSWER: C -- VIRUSES 

A cosmid is a (A) Large bacterial plasmid (B) Viral plasmid (C) Hybrid of plasmid and phage (D) Yeast plasmid

Description : A cosmid is a (A) Large bacterial plasmid (B) Viral plasmid (C) Hybrid of plasmid and phage (D) Yeast plasmid

Last Answer : Answer : C

The plasmid pBR322 has (A) Ampicillin resistance gene (B) Tetracycline resistance gene (C) Both (A) and (B) (D) None of these

Description : The plasmid pBR322 has (A) Ampicillin resistance gene (B) Tetracycline resistance gene (C) Both (A) and (B) (D) None of these

Last Answer : Answer : C

A plasmid is a (A) Single stranded linear DNA (B) Single stranded circular DNA (C) Double stranded linear DNA (D) Double stranded circular DNA

Description : A plasmid is a (A) Single stranded linear DNA (B) Single stranded circular DNA (C) Double stranded linear DNA (D) Double stranded circular DNA

Last Answer : Answer : D

Template-directed DNA synthesis occurs in all the following except (A) The replication fork (B) Polymerase chain reaction (C) Growth of RNA tumor viruses (D) Expression of oncogenes

Description : Template-directed DNA synthesis occurs in all the following except (A) The replication fork (B) Polymerase chain reaction (C) Growth of RNA tumor viruses (D) Expression of oncogenes

Last Answer : Answer : C

Template-directed DNA synthesis occurs in all the following except (A) The replication fork (B) Polymerase chain reaction (C) Growth of RNA tumor viruses (D) Expression of oneogenes

Description : Template-directed DNA synthesis occurs in all the following except (A) The replication fork (B) Polymerase chain reaction (C) Growth of RNA tumor viruses (D) Expression of oneogenes

Last Answer : Answer : C

Expression of structural genes of lac operon is affected by all the following except (A) Lactose or its analogues (B) Repressor tetramer (C) cAMP (D) CAP-cAMP complex

Description : Expression of structural genes of lac operon is affected by all the following except (A) Lactose or its analogues (B) Repressor tetramer (C) cAMP (D) CAP-cAMP complex

Last Answer : Answer : C

We can now test our drinks for the presence of the date rape drug. When might we be able to test our food for E. coli, etc.? See deatil?

Description : We can now test our drinks for the presence of the date rape drug. When might we be able to test our food for E. coli, etc.? See deatil?

Last Answer : …what are your thoughts is more like it.

In E. coli the chain initiating amino acid in protein synthesis is (A) N-formyl methionine(B) Methionine (C) Serine (D) Cysteine

Description : In E. coli the chain initiating amino acid in protein synthesis is (A) N-formyl methionine(B) Methionine (C) Serine (D) Cysteine

Last Answer : Answer : A

DNA ligase of E. coli requires which of the following co-factors? (A) FAD (B) NAD+ (C) NADP+ (D) NADH

Description : DNA ligase of E. coli requires which of the following co-factors? (A) FAD (B) NAD+ (C) NADP+ (D) NADH

Last Answer : Answer : B

Which of the following genes of the E.coli “Lac operon” codes for a constitutive protein? (A) The ‘a’ gene (B) The ‘i’ gene (C) The ‘c’ gene (D) The ‘z’ gene

Description : Which of the following genes of the E.coli “Lac operon” codes for a constitutive protein? (A) The ‘a’ gene (B) The ‘i’ gene (C) The ‘c’ gene (D) The ‘z’ gene

Last Answer : Answer : B

Lac operon of E. coli contains _______ is continuity. (A) Regulator and operator genes only (B) Operator and structural genes only (C) Regular and structural genes only (D) Regulator, operator and structural genes

Description : Lac operon of E. coli contains _______ is continuity. (A) Regulator and operator genes only (B) Operator and structural genes only (C) Regular and structural genes only (D) Regulator, operator and structural genes

Last Answer : Answer : D

DNA po lymerase of T. aquat icus is preferred to that of E. coli in PCR because (A) It replicates DNA more efficiently (B) It doesn’t require primers (C) It is not denatured at the melting temperature of DNA (D) It doesn’t cause errors in replication

Description : DNA po lymerase of T. aquat icus is preferred to that of E. coli in PCR because (A) It replicates DNA more efficiently (B) It doesn’t require primers (C) It is not denatured at the melting temperature of DNA (D) It doesn’t cause errors in replication

Last Answer : Answer : C

The DNA polymerase commonly used in polymerase chain reaction is obtained from (A) E. coli (B) Yeast (C) T.aquaticus (D) Eukaryotes

Description : The DNA polymerase commonly used in polymerase chain reaction is obtained from (A) E. coli (B) Yeast (C) T.aquaticus (D) Eukaryotes

Last Answer : Answer : C

Lac operon is a cluster of genes present in (A) Human beings (B) E. coli (C) Lambda phage (D) All of these

Description : Lac operon is a cluster of genes present in (A) Human beings (B) E. coli (C) Lambda phage (D) All of these

Last Answer : Answer : B

DNA topoisomerase I of E. coli catalyses (A) Relaxation of negatively supercoiled DNA (B) Relaxation of positively supercoiled DNA (C) Conversion of negatively supercoiled DNA into positively supercoiled DNA (D) Conversion of double helix into supercoiled DNA

Description : DNA topoisomerase I of E. coli catalyses (A) Relaxation of negatively supercoiled DNA (B) Relaxation of positively supercoiled DNA (C) Conversion of negatively supercoiled DNA into positively supercoiled DNA (D) Conversion of double helix into supercoiled DNA

Last Answer : Answer : A

The amino terminal of all polypeptide chain at the time of synthesis in E. coli is tagged to the amino acid residue: (A) Methionine (B) Serine (C) N-formyl methinine(D) N-formal serine

Description : The amino terminal of all polypeptide chain at the time of synthesis in E. coli is tagged to the amino acid residue: (A) Methionine (B) Serine (C) N-formyl methinine(D) N-formal serine

Last Answer : Answer : C

What does bacteriophage mean ?

Description : What does bacteriophage mean ?

Last Answer : Bacteriophage: This is basically a type of virus. The specialty of this is that they attack and destroy the bacteria. The viruses that attack bacteria are called bacteriophages. Felix de Harelli discovered bacteriophages.

What is the shape of the head of bacteriophage ?

Description : What is the shape of the head of bacteriophage ?

Last Answer : Bacteriophage's head shape is hexagonal.

What are Bacteriophage lambda found in?

Description : What are Bacteriophage lambda found in?

Last Answer : Need answer

What is a bacteriophage?

Description : What is a bacteriophage?

Last Answer : A bacteriophage is a kind of virus that infects and kills bacteria.a virus that attacks bacteria

The virus that infects a bacterium is (a) Arbovirus (b) Viremia (c) Bacteriophage (d) Baclofen

Description : The virus that infects a bacterium is (a) Arbovirus (b) Viremia (c) Bacteriophage (d) Baclofen

Last Answer : Ans:(c)