The plasmid pBR322 has (A) Ampicillin resistance gene (B) Tetracycline resistance gene (C) Both (A) and (B) (D) None of these

The plasmid pBR322 has (A) Ampicillin resistance gene (B) Tetracycline resistance gene (C) Both (A) and (B) (D) None of these
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31. The BamH I restriction site of the cloning vector pBR322 is situated on ... b) rop gene b) ampicillin resistant gene c) ori gene d) tetracycline resistant gene

Description : 31. The BamH I restriction site of the cloning vector pBR322 is situated on ... b) rop gene b) ampicillin resistant gene c) ori gene d) tetracycline resistant gene

Last Answer : d) tetracycline resistant gene

11. The BamH I restriction site of the cloning vector pBR322 is situated on ... a) rop gene b) ampicillin resistant gene c) ori gene d) tetracycline resistant gene

Description : 11. The BamH I restriction site of the cloning vector pBR322 is situated on ... a) rop gene b) ampicillin resistant gene c) ori gene d) tetracycline resistant gene

Last Answer : d) tetracycline resistant gene

Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes? (a) Retrovirus (b) Ti plasmid (c) λ phage (d) pBR322

Description : Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes? (a) Retrovirus (b) Ti plasmid (c) λ phage (d) pBR322

Last Answer : (a) Retrovirus

Prolonged oral therapy with the following antibiotic can damage intestinal villi resulting in statorrhoea and loose motions (a) Ampicillin (b) Tetracycline (c) Neomycin (d) Nystatin

Description : Prolonged oral therapy with the following antibiotic can damage intestinal villi resulting in statorrhoea and loose motions (a) Ampicillin (b) Tetracycline (c) Neomycin (d) Nystatin

Last Answer : Ans: C

Prolonged oral therapy with the following antibiotic can damage intestinal villi resulting in steatorrhoea and loose motions: A. Ampicillin B. Tetracycline C. Neomycin D. Nystatin

Description : Prolonged oral therapy with the following antibiotic can damage intestinal villi resulting in steatorrhoea and loose motions: A. Ampicillin B. Tetracycline C. Neomycin D. Nystatin

Last Answer : C. Neomycin

A child presented with polyuria, weakness and fever. On the basis of investigations he was labelled to be suffering from Fancony syndrome. A history of taking some old left over antibiotic capsules was obtained. What could have been the antibiotic: A. Tetracycline B. Chloramphenicol C. Ampicillin D. Cephalexi

Description : A child presented with polyuria, weakness and fever. On the basis of investigations he was labelled to be suffering from Fancony syndrome. A history of taking some old left over antibiotic ... What could have been the antibiotic: A. Tetracycline B. Chloramphenicol C. Ampicillin D. Cephalexi

Last Answer : A. Tetracycline

2 Which antibiotic is primarily bacteriostatic but becomes bactericidal at higher concentrations: A. Erythromycin B. Tetracycline C. Chloramphenicol D. Ampicillin

Description : 2 Which antibiotic is primarily bacteriostatic but becomes bactericidal at higher concentrations: A. Erythromycin B. Tetracycline C. Chloramphenicol D. Ampicillin

Last Answer : A. Erythromycin Although erythromycin is primarily bacteriostatic, it is bactericidal at high concentrations for some Gram-positive species, such as group A streptococci and pneumococci

Plasmid has been used as vector because (a) it is circular DNA which have capacity to join to eukaryotic DNA (b) it can move between prokaryotic and eukaryotic cells (c) both ends show replication (d) it has antibiotic resistance gene.

Description : Plasmid has been used as vector because (a) it is circular DNA which have capacity to join to eukaryotic DNA (b) it can move between prokaryotic and eukaryotic cells (c) both ends show replication (d) it has antibiotic resistance gene.

Last Answer : (a) it is circular DNA which have capacity to join to eukaryotic DNA

Antibiotic resistance gene in a plasmid cloning vector -Biology

Description : Antibiotic resistance gene in a plasmid cloning vector -Biology

Last Answer : answer:

Antibiotic resistance gene in plasmid -Biology

Description : Antibiotic resistance gene in plasmid -Biology

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The linking of antibiotic resistance gene with the plasmid vector became possible with (a) DNA polymerase (b) exonucleases (c) DNA ligase (d) endonucleases.

Description : The linking of antibiotic resistance gene with the plasmid vector became possible with (a) DNA polymerase (b) exonucleases (c) DNA ligase (d) endonucleases.

Last Answer : (c) DNA ligase

The given figure is the diagrammatic representation of the E. coli vector pBR322. Which one of the given options correctly identifies its certain component(s)? Bam H1 Sal 1 Pvu II rop ori Pst I Pvu I Eco R I Cla Hin d III tet ampR R (a) ori-original restriction enzyme (b) rop-reduced osmotic pressure (c) HindIII, EcoRI - selectable markers (d) ampR, tetR–antibiotic resistance genes

Description : The given figure is the diagrammatic representation of the E. coli vector pBR322. Which one of the given options correctly identifies its certain component(s)? Bam H1 Sal 1 Pvu II rop ... reduced osmotic pressure (c) HindIII, EcoRI - selectable markers (d) ampR, tetR-antibiotic resistance genes

Last Answer : (d) ampR, tetR–antibiotic resistance genes

Which one of the following vectors is used to replace the defective gene in gene therapy? (a) Adenovirus (b) Cosmid (c) Ri plasmid (d) Ti plasmid

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Last Answer : (a) Adenovirus

The process of replication in plasmid DNA, other than initiation, is controlled by (a) mitochondrial gene (b) plasmid gene (c) bacterial gene (d) none of these.

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Last Answer : c) bacterial gene

In genetic engineering, a DNA segment (gene) of interest, is transferred to the host cell through a vector. Consider the following four agents (i-iv) in this regard and select the correct option about which one or more of these can be used as a vector/vectors. (i) Bacterium (ii) Plasmid (iii)Plasmodium (iv) Bacteriophage (a) (i), (ii) and (iv) (b) (i) only (c) (i) and (iii) (d) (ii) and (iv)

Description : In genetic engineering, a DNA segment (gene) of interest, is transferred to the host cell through a vector. Consider the following four agents (i-iv) in this regard and select the correct option about which one or more of these ... ii) and (iv) (b) (i) only (c) (i) and (iii) (d) (ii) and (iv)

Last Answer : (d) (ii) and (iv)

A gene whose expression helps to identify transformed cell is known as (a) vector (b) plasmid (c) structural gene (d) selectable marker.

Description : A gene whose expression helps to identify transformed cell is known as (a) vector (b) plasmid (c) structural gene (d) selectable marker.

Last Answer : (d) selectable marker.

n bacteria, plasmid is (a) extra chromosomal material (b) main DNA (c) non functional DNA (d) repetitive gene.

Description : n bacteria, plasmid is (a) extra chromosomal material (b) main DNA (c) non functional DNA (d) repetitive gene.

Last Answer : (a) extra chromosomal material

In bacteria, plasmid is (a) extra chromosomal material (b) main DNA (c) non functional DNA (d) repetitive gene.

Description : In bacteria, plasmid is (a) extra chromosomal material (b) main DNA (c) non functional DNA (d) repetitive gene.

Last Answer : (a) extra chromosomal material

Which of the following defines an open reading frame (ORF)? A.A sequence of genome in bacteriophageB.The sequence of a complete genome C.A plasmid vector used in genome sequencing D.A possible gene predicted by DNA sequencing

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Last Answer : D.A possible gene predicted by DNA sequencing

Which of the following defines an open reading frame (ORF)? A- .A sequence of genome in bacteriophage B- The sequence of a complete genome C- A plasmid vector used in genome sequencing D- .A possible gene predicted by DNA sequencing

Description : Which of the following defines an open reading frame (ORF)? A- .A sequence of genome in bacteriophage B- The sequence of a complete genome C- A plasmid vector used in genome sequencing D- .A possible gene predicted by DNA sequencing

Last Answer : .A possible gene predicted by DNA sequencing

Which of the following defines an open reading frame (ORF)? A- .A sequence of genome in bacteriophage B- The sequence of a complete genome C- A plasmid vector used in genome sequencing D- .A possible gene predicted by DNA sequencing

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Last Answer : .A possible gene predicted by DNA sequencing

When composite transposons are formed A- a small deletion occurs in the transposase gene of an IS element B- a small deletion occurs in the transposase gene of an IS element and plasmid is integrated C- an IS element integrates with another IS element with the help of a plasmid D- two IS elements integrate into a chromosome with only a small distance separating them

Description : When composite transposons are formed A- a small deletion occurs in the transposase gene of an IS element B- a small deletion occurs in the transposase gene of an IS element and plasmid is ... a plasmid D- two IS elements integrate into a chromosome with only a small distance separating them

Last Answer : two IS elements integrate into a chromosome with only a small distance separating them

7. In a recombinant DNA technology a plasmid vector must be cleaved by a) the same enzyme that leaves the donor gene b) modified DNA ligase c) a heated alkaline solution d) four separate enzymes

Description : 7. In a recombinant DNA technology a plasmid vector must be cleaved by a) the same enzyme that leaves the donor gene b) modified DNA ligase c) a heated alkaline solution d) four separate enzymes

Last Answer : a) the same enzyme that leaves the donor gene

A gene whose expression helps to identify transformed cell is known as : (1) Vector (2) Plasmid (3) Structural gene (4) Selectable marker

Description : A gene whose expression helps to identify transformed cell is known as : (1) Vector (2) Plasmid (3) Structural gene (4) Selectable marker

Last Answer : 4) Selectable marker

Unidirectional tandem gene arrays are significant in ________ a. Increase in recombinant gene product with increasing plasmid number. b. Increase in recombinant gene product without increasing plasmid number. c. Decrease in polymerase enzyme battery with increasing plasmid numbers. d. Decrease in strong promotor with increase in plasmid number

Description : Unidirectional tandem gene arrays are significant in ________ a. Increase in recombinant gene product with increasing plasmid number. b. Increase in recombinant gene product without increasing plasmid ... increasing plasmid numbers. d. Decrease in strong promotor with increase in plasmid number

Last Answer : b. Increase in recombinant gene product without increasing plasmid number.

In pKO1 plasmid, galactose kinase gene is a reporter gene which lacks ___________ gene. a. Initiation codon b. Promoter c. Activator d. Termination

Description : In pKO1 plasmid, galactose kinase gene is a reporter gene which lacks ___________ gene. a. Initiation codon b. Promoter c. Activator d. Termination

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Multiple antibiotic resistance is mediated by a. Episome b. Plasmid c. Colplasmid d. Both b and c

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Last Answer : Ans: B

The main difference between a self-transmissible and a mobilizableplasmidis that the selftransmissible plasmid A- transfers both strands of the plasmid DNA B- carries genes encoding the mating apparatus C- transfers antibiotic resistance genes D- usually has a transposon inserted into it

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Last Answer : carries genes encoding the mating apparatus

Beta – lactamase production by strains of Haemophilus influenzae, Moraxella catarrhalis, and Neissera gonorrhoeae confers resistance against penicillin G.which one of the following antibiotics is most likely to be effective against all strains of each of the above organisms? (a) Ampicillin (b) Ceftriaxone (c) Clindamycin (d) Gentamicin (e) Piperacillin

Description : Beta - lactamase production by strains of Haemophilus influenzae, Moraxella catarrhalis, and Neissera gonorrhoeae confers resistance against penicillin G.which one of the following antibiotics is most likely to be ... ? (a) Ampicillin (b) Ceftriaxone (c) Clindamycin (d) Gentamicin (e) Piperacillin

Last Answer : Ans: B

Bacteria develop tetracycline resistance by the following mechanisms except: A. Losing tetracycline concentrating mechanisms B. Elaborating tetracycline inactivating enzyme C. Synthesizing a ‘protection protein’ which interferes with binding of tetracycline to the target site D. Actively pumping out tetracycline that has entered the cell

Description : Bacteria develop tetracycline resistance by the following mechanisms except: A. Losing tetracycline concentrating mechanisms B. Elaborating tetracycline inactivating enzyme C. Synthesizing a protection protein' ... the target site D. Actively pumping out tetracycline that has entered the cell

Last Answer : B. Elaborating tetracycline inactivating enzym

The most important mechanism of bacterial resistance to an aminoglycoside antibiotic is (a) Plasmid mediated acquisition of aminoglycoside conjugating enzyme (b) Mutational acquisition of aminoglycoside hydrolyzing enzyme (c) Mutation reducing affinity of ribosomal protein for the antibiotic (d) Mutational loss of porin channels

Description : The most important mechanism of bacterial resistance to an aminoglycoside antibiotic is (a) Plasmid mediated acquisition of aminoglycoside conjugating enzyme (b) Mutational acquisition of aminoglycoside ... affinity of ribosomal protein for the antibiotic (d) Mutational loss of porin channels

Last Answer : Ans: A

The most important mechanism by which gram negative bacilli acquire chloramphenicol resistance is (a) Decreased permeability into the bacterial cell (b) Acquisition of a plasmid encoded for chloramphenicol acetyl transferase (c) Lowered affinity of the bacterial ribosome for chloramphenicol (d) Switching over from ribosomal to mitochondrial protein synthesis

Description : The most important mechanism by which gram negative bacilli acquire chloramphenicol resistance is (a) Decreased permeability into the bacterial cell (b) Acquisition of a plasmid encoded ... bacterial ribosome for chloramphenicol (d) Switching over from ribosomal to mitochondrial protein synthesis

Last Answer : Ans: B

A plasmid carries _____. a. Nonessential Genes b. Resistance Factors c. Genes For Toxinsd. all the above

Description : A plasmid carries _____. a. Nonessential Genes b. Resistance Factors c. Genes For Toxins d. all the above

Last Answer : d. all the above

The plasmid-mediated properties is/are A.fermentation of lactose B.production of enterotoxinC.resistance to antibiotics D.all of these

Description : The plasmid-mediated properties is/are A.fermentation of lactose  B.production of enterotoxin C.resistance to antibiotics  D.all of these

Last Answer : D.all of these

The plasmid-mediated properties is/are A- fermentation of lactose B- production of enterotoxin C- resistance to antibiotics D- all of these

Description : The plasmid-mediated properties is/are A- fermentation of lactose B- production of enterotoxin C- resistance to antibiotics D- all of these

Last Answer : all of these

The most important mechanism by which gram negative bacilli acquire chloramphenicol resistance is: A. Decreased permeability into the bacterial cell B. Acquisition of a plasmid encoded for chloramphenicol acetyl transferase C. Lowered affinity of the bacterial ribosome for chloramphenicol D. Switching over from ribosomal to mitochondrial protein synthesi

Description : The most important mechanism by which gram negative bacilli acquire chloramphenicol resistance is: A. Decreased permeability into the bacterial cell B. Acquisition of a plasmid encoded ... the bacterial ribosome for chloramphenicol D. Switching over from ribosomal to mitochondrial protein synthesi

Last Answer : B. Acquisition of a plasmid encoded for chloramphenicol acetyl transferas

The most common mechanism of development of resistance to fluoroquinolones is: A. Chromosomal mutation altering affinity of target site B. Plasmid transfer C. Acquisition of drug destroying enzyme D. Acquisition of alternative metabolic pathway

Description : The most common mechanism of development of resistance to fluoroquinolones is: A. Chromosomal mutation altering affinity of target site B. Plasmid transfer C. Acquisition of drug destroying enzyme D. Acquisition of alternative metabolic pathway

Last Answer : A. Chromosomal mutation altering affinity of target site

A cosmid is a (A) Large bacterial plasmid (B) Viral plasmid (C) Hybrid of plasmid and phage (D) Yeast plasmid

Description : A cosmid is a (A) Large bacterial plasmid (B) Viral plasmid (C) Hybrid of plasmid and phage (D) Yeast plasmid

Last Answer : Answer : C

Which of the following may be used as a cloning vector? (A) Prokaryotic plasmid(B) Lambda phage (C) Cosmid (D) All of these

Description : Which of the following may be used as a cloning vector? (A) Prokaryotic plasmid(B) Lambda phage (C) Cosmid (D) All of these

Last Answer : Answer : D

A plasmid is a (A) Single stranded linear DNA (B) Single stranded circular DNA (C) Double stranded linear DNA (D) Double stranded circular DNA

Description : A plasmid is a (A) Single stranded linear DNA (B) Single stranded circular DNA (C) Double stranded linear DNA (D) Double stranded circular DNA

Last Answer : Answer : D

All of the following may be used as expression vectors except (A) Plasmid (B) Bacteriophage (C) Baculovirus (D) E. coli

Description : All of the following may be used as expression vectors except (A) Plasmid (B) Bacteriophage (C) Baculovirus (D) E. coli

Last Answer : Answer : D

Highest incidence of antibiotic associated pseudo membranous enterocolitis has been noted with the use of (a) Ampicillin (b) Chloramphenicol (c) Vancomycin (d) Clindamycin

Description : Highest incidence of antibiotic associated pseudo membranous enterocolitis has been noted with the use of (a) Ampicillin (b) Chloramphenicol (c) Vancomycin (d) Clindamycin

Last Answer : Ans: D

An aminoglycoside antibiotic should not be used concurrently with the following drug (a) Ampicillin (b) Vancomycin (c) Ciprofloxacin (d) Rifampin

Description : An aminoglycoside antibiotic should not be used concurrently with the following drug (a) Ampicillin (b) Vancomycin (c) Ciprofloxacin (d) Rifampin

Last Answer : Ans: B

Amoxicillin is inferior to ampicillin for the treatment of the following infection (a) Typhoid (b) Shigella enteritis (c) Subacute bacterial endocarditis (d) Gonorrhoea

Description : Amoxicillin is inferior to ampicillin for the treatment of the following infection (a) Typhoid (b) Shigella enteritis (c) Subacute bacterial endocarditis (d) Gonorrhoea

Last Answer : Ans: B

The most frequent side effect of oral ampicillin is (a) Nausea and vomiting (b) Loose motions (c) Constipation (d) Urticaria

Description : The most frequent side effect of oral ampicillin is (a) Nausea and vomiting (b) Loose motions (c) Constipation (d) Urticaria

Last Answer : Ans: B

Which of the following is not a semisynthetic penicillin ? (a) Procaine penicillin (b) Ampicillin (c) Cloxacillin (d) Carbenicillin

Description : Which of the following is not a semisynthetic penicillin ? (a) Procaine penicillin (b) Ampicillin (c) Cloxacillin (d) Carbenicillin

Last Answer : Ans: A

If a patient gives history of urticaria, itching and swelling of lips following injection of penicillin G, then (a) He will develop similar reaction whenever penicillin is injected (b) He can be given ampicillin safely (c) He can be given oral phenoxymethyl penicillin safely (d) All natural and semisynthetic penicillins are contraindicated for him

Description : If a patient gives history of urticaria, itching and swelling of lips following injection of penicillin G, then (a) He will develop similar reaction whenever penicillin is injected ( ... oral phenoxymethyl penicillin safely (d) All natural and semisynthetic penicillins are contraindicated for him

Last Answer : Ans: D

Currently the drug of choice for emperic treatment of typhoid fever is (a) Chloramphenicol (b) Cotrimoxaxole (c) Ciprofloxacin (d) Ampicillin

Description : Currently the drug of choice for emperic treatment of typhoid fever is (a) Chloramphenicol (b) Cotrimoxaxole (c) Ciprofloxacin (d) Ampicillin

Last Answer : Ans: C

A woman has an upper respiratory infection. Six years ago, she experienced an episode of bronchospasm follwing penicillin V therapy. The cultures now reveal a strain of streptococcus pneumoniae that is sensitive to all of the following drugs. Which of these drugs would be the best choice for this patient? (a) Amoxicillin/clavulanate (b) Erythromycin (c) Ampicillin (d) cefaclor (e) Cyclacillin

Description : A woman has an upper respiratory infection. Six years ago, she experienced an episode of bronchospasm follwing penicillin V therapy. The cultures now reveal a strain of streptococcus pneumoniae that is ... ? (a) Amoxicillin/clavulanate (b) Erythromycin (c) Ampicillin (d) cefaclor (e) Cyclacillin

Last Answer : Ans: B