The DNA polymerase commonly used in polymerase chain reaction is obtained from (A) E. coli (B) Yeast (C) T.aquaticus (D) Eukaryotes

The DNA polymerase commonly used in polymerase chain reaction is obtained from (A) E. coli (B) Yeast (C) T.aquaticus (D) Eukaryotes
by

1 Answer

Answer :

Answer :  C
by
selected

Related questions

Optimum temperature of DNA polymerase of T. aquaticus is (A) 30°C (B) 37°C (C) 54°C (D) 72°C

Description : Optimum temperature of DNA polymerase of T. aquaticus is (A) 30°C (B) 37°C (C) 54°C (D) 72°C

Last Answer : Answer : D

The Taq polymerase enzyme is obtained from (a) Bacillus subtilis (b) Pseudomonas putida (c) Thermus aquaticus (d) Thiobacillus ferroxidans.

Description : The Taq polymerase enzyme is obtained from (a) Bacillus subtilis (b) Pseudomonas putida (c) Thermus aquaticus (d) Thiobacillus ferroxidans.

Last Answer : (c) Thermus aquaticus

Match the organism with its use in biotechnology. (A) Bacillus (i) Cloning vector thuringiensis (B) Thermus (ii) Construction of first aquaticus rDNA molecule (C) Agrobacterium (iii)DNA polymerase tumefaciens (D) Salmonella (iv) Cry proteins typhimurium Select the correct option from the following. (A) (B) (C) (D) (a) (ii) (iv) (iii) (i) (b) (iv) (iii) (i) (ii) (c) (iii) (ii) (iv) (i) (d) (iii) (iv) (i) (ii)

Description : Match the organism with its use in biotechnology. (A) Bacillus (i) Cloning vector thuringiensis (B) Thermus (ii) Construction of first aquaticus rDNA molecule (C) Agrobacterium (iii)DNA polymerase tumefaciens (D) Salmonella (iv) Cry proteins ... (ii) (c) (iii) (ii) (iv) (i) (d) (iii) (iv) (i) (ii)

Last Answer : (b) (iv) (iii) (i) (ii)

α-Amanitin inhibits (A) DNA polymerase II of prokaryotes (B) DNA polymerase α of eukaryotes (C) RNA polymerase II of eukaryotes (D) RNA-dependent DNA polymerase

Description : α-Amanitin inhibits (A) DNA polymerase II of prokaryotes (B) DNA polymerase α of eukaryotes (C) RNA polymerase II of eukaryotes (D) RNA-dependent DNA polymerase

Last Answer : Answer : C

Template-directed DNA synthesis occurs in all the following except (A) The replication fork (B) Polymerase chain reaction (C) Growth of RNA tumor viruses (D) Expression of oncogenes

Description : Template-directed DNA synthesis occurs in all the following except (A) The replication fork (B) Polymerase chain reaction (C) Growth of RNA tumor viruses (D) Expression of oncogenes

Last Answer : Answer : C

Template-directed DNA synthesis occurs in all the following except (A) The replication fork (B) Polymerase chain reaction (C) Growth of RNA tumor viruses (D) Expression of oneogenes

Description : Template-directed DNA synthesis occurs in all the following except (A) The replication fork (B) Polymerase chain reaction (C) Growth of RNA tumor viruses (D) Expression of oneogenes

Last Answer : Answer : C

In addition to Taq polymerase, polymerase chain reaction requires all of the following except (A) A template DNA (B) Deoxyribonucleoside triphosphates (C) Primers (D) Primase

Description : In addition to Taq polymerase, polymerase chain reaction requires all of the following except (A) A template DNA (B) Deoxyribonucleoside triphosphates (C) Primers (D) Primase

Last Answer : Answer : D

Polymerase chain reaction can rapidly amplify DNA sequences of the size (A) Upto 10 kilobases (B) Upto 45 kilobases (C) Upto 100 kilobases(D) Upto 1,000 kilobases

Description : Polymerase chain reaction can rapidly amplify DNA sequences of the size (A) Upto 10 kilobases (B) Upto 45 kilobases (C) Upto 100 kilobases(D) Upto 1,000 kilobases

Last Answer : Answer : A

Most commonly employed test for determining the antibody titers for Newcastle disease infection in B A. Agar gel precipitation test B. Hemagglutination inhibition C. Polymerase chain reaction D. None of these

Description : Most commonly employed test for determining the antibody titers for Newcastle disease infection in B A. Agar gel precipitation test B. Hemagglutination inhibition C. Polymerase chain reaction D. None of these

Last Answer : Hemagglutination inhibition

Most commonly employed test for determining the antibody titers for AI infection is B A. Agar gel precipitation test B. Hemagglutination inhibition C. Polymerase chain reaction D. None of these

Description : Most commonly employed test for determining the antibody titers for AI infection is B A. Agar gel precipitation test B. Hemagglutination inhibition C. Polymerase chain reaction D. None of these

Last Answer : Hemagglutination inhibition

A scientist wants to make a dna fingerprint and she used polymerase chain reaction and restriction enzymes what should her next step be?

Description : A scientist wants to make a dna fingerprint and she used polymerase chain reaction and restriction enzymes what should her next step be?

Last Answer : use restriction enzymes is the right answer for apex :)

A scientist wants to make a dna fingerprint and she used polymerase chain reaction and restriction enzymes what should her next step be?

Description : A scientist wants to make a dna fingerprint and she used polymerase chain reaction and restriction enzymes what should her next step be?

Last Answer : use restriction enzymes is the right answer for apex :)

The following statement(s) is/are true concerning newer detection methods of systemic infection. a. Enzyme-linked immunosorbent assay (ELISA) is a rapid immunologic assay used for both antigen and antibody detection b. Southern, northern, and western immunoblot techniques are used to detect DNA, RNA, or proteins, respectively c. Polymerase chain reaction (PCR) is a sensitive assay used to detect small amounts of microbial DNA, thus detecting infection at its early stages d. Infectious agents currently detected using advanced molecular techniques include cytomegalovirus (CMV) and human immunodeficiency virus (HIV)

Description : The following statement(s) is/are true concerning newer detection methods of systemic infection. a. Enzyme-linked immunosorbent assay (ELISA) is a rapid immunologic assay used for ... detected using advanced molecular techniques include cytomegalovirus (CMV) and human immunodeficiency virus (HIV)

Last Answer : Answer: a, b, c, d Although the classic detection of infection based on clinical signs of infection and bacterial culture remain the most common clinical tools, increasing ... including CMV and HIV. Furthermore, preliminary investigations into possible detection of fungal pathogens are underway

The figure below shows three steps (A, B, C) of Polymerase Chain Reaction (PCR). Select the option giving correct identification together with what it represents? A. B. C ds DNA 3 5 5 3 5 3 5 3 5 3 5 3 5 5 3 3 5 3 5 3 Region to be amplified . (a) B - denaturation at a temperature of about 98°C separating the two DNA strands (b) A - denaturation at a temperature of about 50°C (c) C - extension in the presence of heat stable DNA polymerase (d) A - annealing with two sets of primers

Description : The figure below shows three steps (A, B, C) of Polymerase Chain Reaction (PCR). Select the option giving correct identification together with what it represents? A. B. C ds DNA 3 5 5 3 ... - extension in the presence of heat stable DNA polymerase (d) A - annealing with two sets of primers

Last Answer : (c) C - extension in the presence of heat stable DNA polymerase

DNA fragments generated by the restriction endo- nucleases in a chemical reaction can be separated by (a) electrophoresis (b) restriction mapping (c) centrifugation (d) polymerase chain reaction.

Description : DNA fragments generated by the restriction endo- nucleases in a chemical reaction can be separated by (a) electrophoresis (b) restriction mapping (c) centrifugation (d) polymerase chain reaction.

Last Answer : (a) electrophoresis

Which of the following is not required for any of the techniques of DNA fingerprinting available at present? (a) Restriction enzymes (b) DNA-DNA hybridisation (c) Polymerase chain reaction (d) Zinc finger analysis

Description : Which of the following is not required for any of the techniques of DNA fingerprinting available at present? (a) Restriction enzymes (b) DNA-DNA hybridisation (c) Polymerase chain reaction (d) Zinc finger analysis

Last Answer : (d) Zinc finger analysis

The correct statement concerning RNA and DNA polymerases is (A) RNA polymerase use nucleoside diphosphates (B) RNA polymerase require primers and add bases at 5’ end of the growing polynucleotide chain (C) DNA polymerases can add nucleotides at both ends of the chain (D) All RNA and DNA polymerases can add nucleotides only at the 3’ end of the growing polynucleotide chain

Description : The correct statement concerning RNA and DNA polymerases is (A) RNA polymerase use nucleoside diphosphates (B) RNA polymerase require primers and add bases at 5' end of the growing polynucleotide ... and DNA polymerases can add nucleotides only at the 3' end of the growing polynucleotide chain

Last Answer : Answer : D

DNA po lymerase of T. aquat icus is preferred to that of E. coli in PCR because (A) It replicates DNA more efficiently (B) It doesn’t require primers (C) It is not denatured at the melting temperature of DNA (D) It doesn’t cause errors in replication

Description : DNA po lymerase of T. aquat icus is preferred to that of E. coli in PCR because (A) It replicates DNA more efficiently (B) It doesn’t require primers (C) It is not denatured at the melting temperature of DNA (D) It doesn’t cause errors in replication

Last Answer : Answer : C

acob and Monod studied lactose metabolism in E. coli and proposed operon concept. Operon concept is applicable for (a) all prokaryotes (b) all prokaryotes and some eukaryotes (c) all prokaryotes and all eukaryotes (d) all prokaryotes and some protozoans

Description : acob and Monod studied lactose metabolism in E. coli and proposed operon concept. Operon concept is applicable for (a) all prokaryotes (b) all prokaryotes and some eukaryotes (c) all prokaryotes and all eukaryotes (d) all prokaryotes and some protozoans

Last Answer : (c) all prokaryotes and all eukaryotes

The enzyme used in polymerase chain reaction (PCR) is (A) Taq polymerase (B) RNA polymerase (C) Ribonuclease (D) Endonuclease

Description : The enzyme used in polymerase chain reaction (PCR) is (A) Taq polymerase (B) RNA polymerase (C) Ribonuclease (D) Endonuclease

Last Answer : Answer : D

Types of RNA polymerase required in nucleus of eukaryotes for RNA synthesis (a) 1 (b) 2 (c) 3 (d) 4.

Description : Types of RNA polymerase required in nucleus of eukaryotes for RNA synthesis (a) 1 (b) 2 (c) 3 (d) 4.

Last Answer : 1

Types of RNA polymerase required in nucleus of eukaryotes for RNA synthesis (a) 1 (b) 2 (c) 3 (d) 4.

Description : Types of RNA polymerase required in nucleus of eukaryotes for RNA synthesis (a) 1 (b) 2 (c) 3 (d) 4.

Last Answer : (c) 3

The removal of which enzyme affects the synthesis of hnRNA in eukaryotes a) RNA polymerase II b) RNA primase c) RNA polymerase III d) RNA polymerase I

Description : The removal of which enzyme affects the synthesis of hnRNA in eukaryotes a) RNA polymerase II b) RNA primase c) RNA polymerase III d) RNA polymerase I

Last Answer : a) RNA polymerase II

Puromycin causes premature chain termination in (A) Prokaryotes (B) Eukaryotes (C) Both (A) and (B) (D) None of these

Description : Puromycin causes premature chain termination in (A) Prokaryotes (B) Eukaryotes (C) Both (A) and (B) (D) None of these

Last Answer : Answer : C

The α-amino group of the new amino acyl tRNA in the A site carries out a nucleophilic attack on the esterified carboxyl group of the peptidyl tRNA occupying the P site. This reaction is catalysed by (A) DNA polymerase (B) RNA polymerase (C) Peptidyl transferase (D) DNA ligase

Description : The α-amino group of the new amino acyl tRNA in the A site carries out a nucleophilic attack on the esterified carboxyl group of the peptidyl tRNA occupying the P site. This reaction is catalysed by (A) DNA polymerase (B) RNA polymerase (C) Peptidyl transferase (D) DNA ligase

Last Answer : Answer : C

In E. coli the chain initiating amino acid in protein synthesis is (A) N-formyl methionine(B) Methionine (C) Serine (D) Cysteine

Description : In E. coli the chain initiating amino acid in protein synthesis is (A) N-formyl methionine(B) Methionine (C) Serine (D) Cysteine

Last Answer : Answer : A

The amino terminal of all polypeptide chain at the time of synthesis in E. coli is tagged to the amino acid residue: (A) Methionine (B) Serine (C) N-formyl methinine(D) N-formal serine

Description : The amino terminal of all polypeptide chain at the time of synthesis in E. coli is tagged to the amino acid residue: (A) Methionine (B) Serine (C) N-formyl methinine(D) N-formal serine

Last Answer : Answer : C

In addition to DNA polymerase and primers what else does a polymerase chain require?

Description : In addition to DNA polymerase and primers what else does a polymerase chain require?

Last Answer : Need answer

The DNA polymerase enzyme used in PCR is obtained from

Description : 8. The DNA polymerase enzyme used in PCR is obtained from (a) Thermus aquaticus (b) Escherichia coli (c) Agrobacterium tumefaciens (d) Salmonella typhimurium.

Last Answer : (a) Thermus aquaticus

The DNA polymerase enzyme used in PCR is obtained from

Description : 6. The DNA polymerase enzyme used in PCR is obtained from (a) Thermusaquaticus (b) Escherichia coli (c) Agrobacterium tumefaciens (d) Salmonella typhimurium

Last Answer : (c) Agrobacterium tumefaciens

Ciprofloxacin inhibits the synthesis of (A) DNA in prokaryotes (B) DNA in prokaryotes and eukaryotes (C) RNA in prokaryotes (D) RNA in prokaryotes and eukaryotes

Description : Ciprofloxacin inhibits the synthesis of (A) DNA in prokaryotes (B) DNA in prokaryotes and eukaryotes (C) RNA in prokaryotes (D) RNA in prokaryotes and eukaryotes

Last Answer : Answer : A

Some DNA is present in mitochondria of (A) Prokaryotes (B) Eukaryotes (C) Both (A) and (B) (D) None of these

Description : Some DNA is present in mitochondria of (A) Prokaryotes (B) Eukaryotes (C) Both (A) and (B) (D) None of these

Last Answer : Answer : B

Mitochondrial DNA is present in (A) Bacteria (B) Viruses (C) Eukaryotes (D) All of these

Description : Mitochondrial DNA is present in (A) Bacteria (B) Viruses (C) Eukaryotes (D) All of these

Last Answer : Answer : C

DNA ligase of E. coli requires which of the following co-factors? (A) FAD (B) NAD+ (C) NADP+ (D) NADH

Description : DNA ligase of E. coli requires which of the following co-factors? (A) FAD (B) NAD+ (C) NADP+ (D) NADH

Last Answer : Answer : B

DNA topoisomerase I of E. coli catalyses (A) Relaxation of negatively supercoiled DNA (B) Relaxation of positively supercoiled DNA (C) Conversion of negatively supercoiled DNA into positively supercoiled DNA (D) Conversion of double helix into supercoiled DNA

Description : DNA topoisomerase I of E. coli catalyses (A) Relaxation of negatively supercoiled DNA (B) Relaxation of positively supercoiled DNA (C) Conversion of negatively supercoiled DNA into positively supercoiled DNA (D) Conversion of double helix into supercoiled DNA

Last Answer : Answer : A

What are the requirements for PCR (Polymerase Chain Reaction)? -Biology

Description : What are the requirements for PCR (Polymerase Chain Reaction)? -Biology

Last Answer : answer:

Steps involved in PCR (Polymerase Chain Reaction) process. -Biology

Description : Steps involved in PCR (Polymerase Chain Reaction) process. -Biology

Last Answer : Step 1 DNA isolation.Step 2 Primer design.Step 3 Enzyme selection.Step 4 Thermal cycling.Step 5 Amplicon analysis.

Why is polymerase chain reaction important? -Biology

Description : Why is polymerase chain reaction important? -Biology

Last Answer : Polymerase chain reaction (PCR) is often considered as one of the most important scientific advances in the field of molecular biology. With this revolutionary yet inexpensive biochemical technology, it's possible to generate millions of DNA copies from a single strand of DNA.

Why is polymerase chain reaction important? -Biology

Description : Why is polymerase chain reaction important? -Biology

Last Answer : Polymerase chain reaction (PCR) is often considered as one of the most important scientific advances in the field of molecular biology. With this revolutionary yet inexpensive biochemical technology, it's possible to generate millions of DNA copies from a single strand of DNA.

What does polymerase chain reaction (pcr) do?

Description : What does polymerase chain reaction (pcr) do?

Last Answer : Polymerase chain reaction, or PCR, is a laboratory technique used to make multiple copies of a segment of DNA. PCR is very precise and can be used to amplify, or copy, a specific DNA target from a mixture of DNA molecules.

Trichomoniasis can be diagnosed by D A. Whiff test B. Polymerase chain reaction C. Wet mount D. All above

Description : Trichomoniasis can be diagnosed by D A. Whiff test B. Polymerase chain reaction C. Wet mount D. All above

Last Answer : All above

The correct order of steps in Polymerase Chain Reaction (PCR) is (a) extension, denaturation, annealing (b) annealing, extension, denaturation (c) denaturation, extension, annealing (d) denaturation, annealing, extension.

Description : The correct order of steps in Polymerase Chain Reaction (PCR) is (a) extension, denaturation, annealing (b) annealing, extension, denaturation (c) denaturation, extension, annealing (d) denaturation, annealing, extension.

Last Answer : (d) denaturation, annealing, extension.

The following are useful in the diagnosis of HIV infection: a. polymerase chain reaction b. antibodies by enzyme-liked immunoadsorbent assay c. P24 protein assay d. all above

Description : The following are useful in the diagnosis of HIV infection: a. polymerase chain reaction b. antibodies by enzyme-liked immunoadsorbent assay c. P24 protein assay d. all above

Last Answer : all above

Which of the following infection(s) can be diagnosed by the use of polymerase chain reaction? A- HIV-1 and HIV-2 viruses B- Hepatitis B virus C- Mycobacterium tuberculosis D- All of these

Description : Which of the following infection(s) can be diagnosed by the use of polymerase chain reaction? A- HIV-1 and HIV-2 viruses B- Hepatitis B virus C- Mycobacterium tuberculosis D- All of these

Last Answer : All of these

An important method used in the rapid identification of a pathogen is a. RNA gene sequencing b. polymerase chain reaction c. molecular taxonomy d. biochemical tests

Description : An important method used in the rapid identification of a pathogen is a. RNA gene sequencing b. polymerase chain reaction c. molecular taxonomy d. biochemical tests

Last Answer : d. biochemical tests

The correct order of steps in Polymerase Chain Reaction (PCR) is (1) Extension, Denaturation, Annealing (2) Annealing, Extension, Denaturation (3) Denaturation, Extension, Annealing (4) Denaturation, Annealing, Extension

Description : The correct order of steps in Polymerase Chain Reaction (PCR) is (1) Extension, Denaturation, Annealing (2) Annealing, Extension, Denaturation (3) Denaturation, Extension, Annealing (4) Denaturation, Annealing, Extension

Last Answer : (4) Denaturation, Annealing, Extension

Non-pathogenic species of genus Entamoeba occurs commonly in the human mouth is B A. E. coli B. E. gingivalis C. E. hartmanni D. None

Description : Non-pathogenic species of genus Entamoeba occurs commonly in the human mouth is B A. E. coli B. E. gingivalis C. E. hartmanni D. None

Last Answer : E. gingivalis

Most commonly used organism in SCP is (a) Bacillus (b) E. coli (c) Spirulina (d) Pseudomonas

Description : Most commonly used organism in SCP is (a) Bacillus (b) E. coli (c) Spirulina (d) Pseudomonas

Last Answer : (c) Spirulina

Which one of the following organisms is scientifically correctly named, correctly printed according to the International Rules of Nomenclature and correctly described? (a) Musca domestica - the common house lizard, a reptile (b) Plasmodium falciparum - a protozoan pathogen causing the most serious type of malaria. (c) Felis tigris - the Indian tiger, well protected in Gir forests. (d) E.coli - full name Entamoeba coli, a commonly occurring bacterium in human intestine.

Description : Which one of the following organisms is scientifically correctly named, correctly printed according to the International Rules of Nomenclature and correctly described? (a) Musca domestica - the common ... ) E.coli - full name Entamoeba coli, a commonly occurring bacterium in human intestine.

Last Answer : (b) Plasmodium falciparum - a protozoan pathogen causing the most serious type of malaria

Yeast artificial chromosome can be used to amplify DNA sequences of the size (A) Upto 10 kb (B) Upto 45 kb (C) Upto 100 kb (D) Upto 1,000 kb

Description : Yeast artificial chromosome can be used to amplify DNA sequences of the size (A) Upto 10 kb (B) Upto 45 kb (C) Upto 100 kb (D) Upto 1,000 kb

Last Answer : Answer : D