Description : Template-directed DNA synthesis occurs in all the following except (A) The replication fork (B) Polymerase chain reaction (C) Growth of RNA tumor viruses (D) Expression of oneogenes
Last Answer : Answer : C
Description : All of the following statements about RNA-dependent DNA polymerase are true except: (A) It synthesizes DNA using RNA as a template (B) It is also known as reverse transcriptase (C) It synthesizes DNA in 5’→3’ direction (D) It is present in all the viruses
Last Answer : Answer : D
Description : At a chromosome replication fork, the lagging strand consists of__ That is joined by___. a. RNA sequences; DNA ligase b. Okazaki Fragments; RNA Polymerase c. RNA sequences; ribosomes d. Okazaki fragments; DNA ligase
Last Answer : d. Okazaki fragments; DNA ligase
Description : In addition to Taq polymerase, polymerase chain reaction requires all of the following except (A) A template DNA (B) Deoxyribonucleoside triphosphates (C) Primers (D) Primase
Description : The enzyme adds complementary bases to the DNA template strand during replication. a. Ligase b. Helicase c. DNA polymerase III d. RNA polymerase
Last Answer : d. RNA polymerase
Description : Synthesis of RNA and a DNA template is known as (A) Replication (B) Translation (C) Transcription (D) Mutation
Description : The only correct statement about oncoviruses is (A) All the oncoviruses are RNA viruses (B) Reverse transcriptase is present in all oncoviruses (C) Viral oncogenes are identical to human protooncogens (D) Both DNA and RNA viruses can be oncoviruses
Description : After formation of replication fork (A) Both the new strands are synthesized discontinuously (B) One strand is synthesized continuously and the other discontinuously (C) Both the new strands are synthesized continuously (D) RNA primer is required only for the synthesis of one new strand
Last Answer : Answer : B
Description : In DNA replication the enzyme required in the first step is (A) DNA directed polymerase (B) Unwinding proteins (C) DNA polymerase (D) DNA ligase
Description : There are special proteins that help to open up DNA double helix in front of the replication fork. These proteins are (a) DNA ligase (b) DNA topoisomerase I (c) DNA gyrase (d) DNA polymerase I.
Last Answer : (c) DNA gyrase
Description : RNA directed DNA polymerase is (A) Replicase (B) Transcriptase (C) Reversetranscriptase (D) Polymerase–III
Description : DNA directed RNA polymerase is (A) Replicase (B) Transcriptase (C) Reverse transcriptase (D) Polymerase III
Description : What is antisense technology? (a) When a piece of RNA that is complementary in sequence is used to stop expression of a specific gene (b) RNA polymerase producing DNA (c) A cell displaying a foreign antigen used for synthesis of antigens (d) Production of somaclonal variants in tissue cultures
Last Answer : (a) When a piece of RNA that is complementary in sequence is used to stop expression of a specific gene
Description : Lactose or its analogues act as positive regulators of lac operon by (A) Attaching to i gene and preventing its expression (B) Increasing the synthesis of catabolite gene activator protein (C) ... holoenzyme (D) Binding to repressor subunits so that the repressor cannot attach to the operator locus
Description : DNA-dependent RNA polymerase catalyses transcription on one strand of the DNA which is called the (a) template strand (b) coding strand (c) alpha strand (d) antistrand
Last Answer : (a) template strand
Description : DNA-dependent RNA polymerase catalyses transcription on one strand of the DNA which is called the (a) template strand (b) coding strand (c) alpha strand (d) antistrand.
Description : Which one of the following makes use of RNA template to synthesise DNA? (a) DNA polymerase (b) RNA polymerase (c) Reverse transcriptase (d) DNA dependant RNA polymerase
Last Answer : c) Reverse transcriptase
Description : RNA synthesis requires (A) RNA primer (B) RNA template (C) DNA template (D) DNA primer
Description : After termination of the synthesis of RNA molecule, the core enzymes separate from the DNA template. The core enzymes then recognize a promoter at which the synthesis of a new RNA molecule commences, with the assistance of (A) Rho (ρ) factor (B) δ factor (C) β factor (D) σ factor
Description : Termination of the synthesis of the RNA molecule is signaled by a sequence in the template strand of the DNA molecule, a signal that is recognized by a termination protein, the (A) Rho (ρ) factor (B) σ factor (C) δ factor (D) ε factor
Last Answer : Answer : A
Description : 9 In some viruses DNA is synthesized by using RNA as a template. Such a DNA is called............ a. r-DNA b. C-DNA c. B- DNA d. A- DNA
Last Answer : Ans. b C-DNA
Description : Translocase is an enzyme required in the process of (A) DNA replication (B) RNA synthesis (C) Initiation of protein synthesis (D) Elongation of peptides
Description : The following statement(s) is/are true concerning newer detection methods of systemic infection. a. Enzyme-linked immunosorbent assay (ELISA) is a rapid immunologic assay used for ... detected using advanced molecular techniques include cytomegalovirus (CMV) and human immunodeficiency virus (HIV)
Last Answer : Answer: a, b, c, d Although the classic detection of infection based on clinical signs of infection and bacterial culture remain the most common clinical tools, increasing ... including CMV and HIV. Furthermore, preliminary investigations into possible detection of fungal pathogens are underway
Description : The correct statement concerning RNA and DNA polymerases is (A) RNA polymerase use nucleoside diphosphates (B) RNA polymerase require primers and add bases at 5' end of the growing polynucleotide ... and DNA polymerases can add nucleotides only at the 3' end of the growing polynucleotide chain
Description : The enzyme used in polymerase chain reaction (PCR) is (A) Taq polymerase (B) RNA polymerase (C) Ribonuclease (D) Endonuclease
Description : Select the correct option. Direction of Direction of reading of RNA synthesis the template DNA strand (a) 5′ – 3′ 3′ – 5′ (b) 3′ – 5′ 5′ – 3′ (c) 5′ – 3′ 5′ – 3′ (d) 3′ – 5′ 3′ – 5′
Last Answer : (d) 3′ – 5′ 3′ – 5′
Last Answer : (a) 5′ – 3′ 3′ – 5′
Description : An important step in protein synthesis is transcription. Which of the following statement(s) is/are true concerning this process? a. The first step in gene transcription involves separating the double helix ... nucleus to the cytoplasm d. Only one protein can be produced from an initial mRNA strand
Last Answer : Answer: c Transcription of a gene begins at an initiation site associated with a specific DNA sequence, termed a promoter region. After binding to DNA, the RNA polymerase opens up a short ... different proteins from the same gene. mRNA is exported from the nucleus only after processing is complete
Description : Negative regulation of protein synthesis is accomplished by A- allosteric inhibition B- the binding of RNA polymerase to the promoter C- the binding of a repressor to the DNA D- the binding of a repressor to the RNA polymerase
Last Answer : the binding of a repressor to the DNA
Description : Rifampin kills tubercle bacilli by: A. Binding to mycobacterial DNA dependent RNA polymerase B. Inhibiting mycobacterial DNA synthesis C. Inhibiting synthesis of mycolic acids in mycobacteria D. Damaging mycobacterial mitochondria
Last Answer : A. Binding to mycobacterial DNA dependent RNA polymerase
Description : During replication, the template DNA is unwound (A) At one of the ends (B) At both the ends (C) At multiple sites (D) Nowhere
Description : The α-amino group of the new amino acyl tRNA in the A site carries out a nucleophilic attack on the esterified carboxyl group of the peptidyl tRNA occupying the P site. This reaction is catalysed by (A) DNA polymerase (B) RNA polymerase (C) Peptidyl transferase (D) DNA ligase
Description : .After a mutation at a genetic locus the character of an organism changes due to change in (a) protein structure (b) DNA replication (c) protein synthesis pattern (d) RNA transcription pattern.
Last Answer : (d) RNA transcription pattern.
Description : After a mutation at a genetic locus the character of an organism changes due to change in (a) protein structure (b) DNA replication (c) protein synthesis pattern (d) RNA transcription pattern.
Last Answer : a) protein structure
Description : .Balbiani rings (puffs) are sites of (a) DNA replication (b) RNA and protein synthesis (c) synthesis of polysaccharides (d) synthesis of lipids.
Last Answer : (b) RNA and protein synthesis
Description : Which of the following infection(s) can be diagnosed by the use of polymerase chain reaction? A- HIV-1 and HIV-2 viruses B- Hepatitis B virus C- Mycobacterium tuberculosis D- All of these
Last Answer : All of these
Description : DNA contains some palindromic sequences which (A) Mark the site for the formation of replication forks (B) Direct DNA polymerase to turn back to replicate the other strand (C) Are recognized by restriction enzymes (D) Are found only in bacterial DNA
Description : Replication of nuclear DNA in mammals is catalysed by (A) DNA polymerase α (B) DNA polymerase β (C) DNA polymerase γ (D) DNA polymerase III
Description : 3’→5’ Exonuclease activity of DNA polymerase I (A) Removes ribonucleotides (B) Adds deoxyribonucleotides (C) Corrects errors in replication (D) Hydrolyses DNA into mononucleotides
Description : The DNA polymerase commonly used in polymerase chain reaction is obtained from (A) E. coli (B) Yeast (C) T.aquaticus (D) Eukaryotes
Description : Polymerase chain reaction can rapidly amplify DNA sequences of the size (A) Upto 10 kilobases (B) Upto 45 kilobases (C) Upto 100 kilobases(D) Upto 1,000 kilobases
Description : The mechanism of synthesis of DNA and RNA are similar to all the following ways except (A) They involve release of pyrophosphate from each nucleotide added (B) They require activated nucleotide precursor and Mg2+ (C) The direction of synthesis is (D) They require a primer
Description : The mechanism of synthesis of DNA and RNA are similar in all the following ways except (A) They involve release of pyrophosphate from each nucleotide added (B) They require activated nucleotide precursor and Mg2+ (C) The direction of synthesis is 5’ → 3’ (D) They require a primer
Description : In mammals, synthesis of mRNA is catalysed by (A) RNA polymerase I (B) RNA polymerase II (C) RNA polymerase III(D) RNA polymerase IV
Description : The function of a repressor protein in an operon system is to prevent synthesis by binding to (A) The ribosome (B) A specific region of the operon preventing transcription of structural genes (C) The RNA polymerase (D) A specific region of the mRNA preventing translation to protein
Description : An important method used in the rapid identification of a pathogen is a. RNA gene sequencing b. polymerase chain reaction c. molecular taxonomy d. biochemical tests
Last Answer : d. biochemical tests
Description : What is DNA replication fork? -Biology
Last Answer : answer:
Description : During DNA replication, Okazaki fragments are used to elongate (a) the lagging strand towards replication fork (b) the leading strand away from replication fork (c) the lagging strand away from the replication fork (d) the leading strand towards replication fork.
Last Answer : (b) the leading strand away from replication fork
Description : When cell has stalled DNA replication fork, which checkpoint should be predominantly activated? (a) G1/S (b) G2/M (c) M (d) Both G2/M and M
Last Answer : b) G2/M
Description : During DNA replication, Okazaki fragments are used to elongate: (1) The lagging strand towards replication fork. (2) The leading strand away from replication fork. (3) The lagging strand away from the replication fork. (4) The leading strand towards replication fork.
Last Answer : (3) The lagging strand away from the replication fork.