DefinitionGram stain of tissue biopsy test involves using crystal violet stain to test a sample of tissue taken from a biopsy.The Gram stain method can be applied to almost any clinical specimen and is an excellent technique for making a general, basic identification of the type of bacteria present in the sample.How the test is performedA sample, called a smear, from a tissue specimen is usually applied in a very thin layer to a microscope slide. The specimen is stained with crystal violet stain and undergoes additional processing before it is examined under the microscope for the presence of microorganisms. Different characteristics of the microoganisms, such as color, shape and pattern of staining, help determine the type of microorganism.How to prepare for the testIf the biopsy is inclu