Description : Plasmid has been used as vector because (a) it is circular DNA which have capacity to join to eukaryotic DNA (b) it can move between prokaryotic and eukaryotic cells (c) both ends show replication (d) it has antibiotic resistance gene.
Last Answer : (a) it is circular DNA which have capacity to join to eukaryotic DNA
Description : The linking of antibiotic resistance gene with the plasmid vector became possible with (a) DNA polymerase (b) exonucleases (c) DNA ligase (d) endonucleases.
Last Answer : (c) DNA ligase
Description : Antibiotic resistance gene in plasmid -Biology
Last Answer : answer:
Description : Which of the following may be used as a cloning vector? (A) Prokaryotic plasmid(B) Lambda phage (C) Cosmid (D) All of these
Last Answer : Answer : D
Description : The DNA molecule to which the gene of interest is integrated for cloning is called (a) template (b) carrier (c) transformer (d) vector.
Last Answer : (d) vector.
Description : 31. The BamH I restriction site of the cloning vector pBR322 is situated on ... b) rop gene b) ampicillin resistant gene c) ori gene d) tetracycline resistant gene
Last Answer : d) tetracycline resistant gene
Description : 11. The BamH I restriction site of the cloning vector pBR322 is situated on ... a) rop gene b) ampicillin resistant gene c) ori gene d) tetracycline resistant gene
Description : In genetic engineering, a DNA segment (gene) of interest, is transferred to the host cell through a vector. Consider the following four agents (i-iv) in this regard and select the correct option about which one or more of these ... ii) and (iv) (b) (i) only (c) (i) and (iii) (d) (ii) and (iv)
Last Answer : (d) (ii) and (iv)
Description : A gene whose expression helps to identify transformed cell is known as (a) vector (b) plasmid (c) structural gene (d) selectable marker.
Last Answer : (d) selectable marker.
Description : Which of the following defines an open reading frame (ORF)? A.A sequence of genome in bacteriophage B.The sequence of a complete genome C.A plasmid vector used in genome sequencing D.A possible gene predicted by DNA sequencing
Last Answer : D.A possible gene predicted by DNA sequencing
Description : Which of the following defines an open reading frame (ORF)? A- .A sequence of genome in bacteriophage B- The sequence of a complete genome C- A plasmid vector used in genome sequencing D- .A possible gene predicted by DNA sequencing
Last Answer : .A possible gene predicted by DNA sequencing
Description : 7. In a recombinant DNA technology a plasmid vector must be cleaved by a) the same enzyme that leaves the donor gene b) modified DNA ligase c) a heated alkaline solution d) four separate enzymes
Last Answer : a) the same enzyme that leaves the donor gene
Description : A gene whose expression helps to identify transformed cell is known as : (1) Vector (2) Plasmid (3) Structural gene (4) Selectable marker
Last Answer : 4) Selectable marker
Description : Multiple antibiotic resistance is mediated by a. Episome b. Plasmid c. Colplasmid d. Both b and c
Last Answer : Ans: B
Description : The most important mechanism of bacterial resistance to an aminoglycoside antibiotic is (a) Plasmid mediated acquisition of aminoglycoside conjugating enzyme (b) Mutational acquisition of aminoglycoside ... affinity of ribosomal protein for the antibiotic (d) Mutational loss of porin channels
Last Answer : Ans: A
Description : The main difference between a self-transmissible and a mobilizableplasmidis that the selftransmissible plasmid A- transfers both strands of the plasmid DNA B- carries genes encoding the mating apparatus C- transfers antibiotic resistance genes D- usually has a transposon inserted into it
Last Answer : carries genes encoding the mating apparatus
Description : The plasmid pBR322 has (A) Ampicillin resistance gene (B) Tetracycline resistance gene (C) Both (A) and (B) (D) None of these
Last Answer : Answer : C
Description : Antibiotic resistance gene in bacteria -Biology
Description : Antibiotic resistance gene function -Biology
Description : Antibiotic resistance gene expression -Biology
Description : Antibiotic resistance horizontal gene transfer -Biology
Description : Antibiotic resistance gene list -Biology
Description : What are the features of cloning vector? -Biology
Description : What is cloning vector? -Biology
Description : Difference between plasmid and vector. -Biology
Description : Difference between plasmid and expression vector. -Biology
Description : The given figure is the diagrammatic representation of the E. coli vector pBR322. Which one of the given options correctly identifies its certain component(s)? Bam H1 Sal 1 Pvu II rop ... reduced osmotic pressure (c) HindIII, EcoRI - selectable markers (d) ampR, tetR-antibiotic resistance genes
Last Answer : (d) ampR, tetR–antibiotic resistance genes
Description : What is gene cloning? -Biology
Description : What is meant by gene cloning? -Biology
Description : The most important mechanism of bacterial resistance to an aminoglycoside antibiotic is: A. Plasmid mediated acquisition of aminoglycoside conjugating enzyme B. Mutational acquisition of aminoglycoside ... affinity of ribosomal protein for the antibiotic D. Mutational loss of porin channels
Last Answer : A. Plasmid mediated acquisition of aminoglycoside conjugating enzyme
Description : Transposons are of particular significance because they a. often contain genes for antibiotic resistance. b. inhibit the effects of overlapping genes. c. come in pairs and often are associated with viruses. d. regulate gene transcription in bacterial cells.
Last Answer : a. often contain genes for antibiotic resistance.
Description : Match the organism with its use in biotechnology. (A) Bacillus (i) Cloning vector thuringiensis (B) Thermus (ii) Construction of first aquaticus rDNA molecule (C) Agrobacterium (iii)DNA polymerase tumefaciens (D) Salmonella (iv) Cry proteins ... (ii) (c) (iii) (ii) (iv) (i) (d) (iii) (iv) (i) (ii)
Last Answer : (b) (iv) (iii) (i) (ii)
Description : Which one of the following is used as vector for cloning genes into higher organisms? (a) Baculovirus (b) Salmonella typhimurium (c) Rhizopus nigricans (d) Retrovirus
Last Answer : (d) Retrovirus
Description : 4. ———– is used as a vector for cloning into higher organisms a. Retrovirus b. Baculovirus c. Salmonella typhimurium d. Rhizopusnigricans
Last Answer : a. Retrovirus
Description : Plasmid vector in DNA recombinant technology means
Last Answer : Plasmid vector in DNA recombinant technology means A. a virus that transfers gene to bacteria B. ... D. any fragment of DNA carrying desirable gene
Description : DNA or RNA segment tagged with a radioactive molecule is called (a) vector (b) probe (c) clone (d) plasmid.
Last Answer : (b) probe
Description : A single strand of nucleic acid tagged with a radioactive molecule is called (a) vector (b) selectable marker (c) plasmid (d) probe
Last Answer : d) probe.
Description : Which vector can clone only a small fragment of DNA? (a) Bacterial artificial chromosome (b) Yeast artificial chromosome (c) Plasmid (d) Cosmid
Last Answer : (c) Plasmid
Description : Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes? (a) Retrovirus (b) Ti plasmid (c) λ phage (d) pBR322
Last Answer : (a) Retrovirus
Description : Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes ? (1) Retrovirus (2) Ti plasmid (3) phage (4) pBR 322
Last Answer : (1) Retrovirus
Description : 71. A single strand of nucleic acid tagged with a radioactive molecule is called (1) Probe (2) Vector (3) Selectable marker (4) Plasmid
Last Answer : Probe
Description : What is Gene Cloning ?
Last Answer : Gene cloning is the process of making multiple copies of the same gene.
Description : Which of the following are used in gene cloning? (a) Nucleoids (b) Lomasomes (c) Mesosomes (d) Plasmids
Last Answer : (d) Plasmids
Description : A microarray differs from a gene fusion in that, it A- carries DNA segments from many different genes B- is not constructed by cloning C- gives direct measurement of mRNA level D- all of the above
Last Answer : all of the above
Description : The aim of genomic medicine is a) improving predictions about individuals' susceptibility to diseases Predictions regarding the time of their onset, their extent and eventual severity as well as which ... broader than the cure of disease. d) cloning Genomic medicine is gene-based health care.
Last Answer : a) improving predictions about individuals’ susceptibility to diseases Predictions regarding the time of their onset, their extent and eventual severity as well as which treatments or medications are likely to be most effective or harmful are the focus of genomic medicine.
Description : 12. . During “gene cloning” which is called as “gene taxi”? a. Vaccine b. Plasmid c. Bacterium d. Protozoa
Last Answer : b. Plasmid
Description : Which one of the following vectors is used to replace the defective gene in gene therapy? (a) Adenovirus (b) Cosmid (c) Ri plasmid (d) Ti plasmid
Last Answer : (a) Adenovirus
Description : The process of replication in plasmid DNA, other than initiation, is controlled by (a) mitochondrial gene (b) plasmid gene (c) bacterial gene (d) none of these.
Last Answer : c) bacterial gene
Description : n bacteria, plasmid is (a) extra chromosomal material (b) main DNA (c) non functional DNA (d) repetitive gene.
Last Answer : (a) extra chromosomal material