Description : Antibiotic resistance gene in a plasmid cloning vector -Biology
Last Answer : answer:
Description : Plasmid has been used as vector because (a) it is circular DNA which have capacity to join to eukaryotic DNA (b) it can move between prokaryotic and eukaryotic cells (c) both ends show replication (d) it has antibiotic resistance gene.
Last Answer : (a) it is circular DNA which have capacity to join to eukaryotic DNA
Description : The linking of antibiotic resistance gene with the plasmid vector became possible with (a) DNA polymerase (b) exonucleases (c) DNA ligase (d) endonucleases.
Last Answer : (c) DNA ligase
Description : Multiple antibiotic resistance is mediated by a. Episome b. Plasmid c. Colplasmid d. Both b and c
Last Answer : Ans: B
Description : The most important mechanism of bacterial resistance to an aminoglycoside antibiotic is (a) Plasmid mediated acquisition of aminoglycoside conjugating enzyme (b) Mutational acquisition of aminoglycoside ... affinity of ribosomal protein for the antibiotic (d) Mutational loss of porin channels
Last Answer : Ans: A
Description : The main difference between a self-transmissible and a mobilizableplasmidis that the selftransmissible plasmid A- transfers both strands of the plasmid DNA B- carries genes encoding the mating apparatus C- transfers antibiotic resistance genes D- usually has a transposon inserted into it
Last Answer : carries genes encoding the mating apparatus
Description : The plasmid pBR322 has (A) Ampicillin resistance gene (B) Tetracycline resistance gene (C) Both (A) and (B) (D) None of these
Last Answer : Answer : C
Description : Antibiotic resistance gene in bacteria -Biology
Description : Antibiotic resistance gene function -Biology
Description : Antibiotic resistance gene expression -Biology
Description : Antibiotic resistance horizontal gene transfer -Biology
Description : Antibiotic resistance gene list -Biology
Description : The most important mechanism of bacterial resistance to an aminoglycoside antibiotic is: A. Plasmid mediated acquisition of aminoglycoside conjugating enzyme B. Mutational acquisition of aminoglycoside ... affinity of ribosomal protein for the antibiotic D. Mutational loss of porin channels
Last Answer : A. Plasmid mediated acquisition of aminoglycoside conjugating enzyme
Description : Transposons are of particular significance because they a. often contain genes for antibiotic resistance. b. inhibit the effects of overlapping genes. c. come in pairs and often are associated with viruses. d. regulate gene transcription in bacterial cells.
Last Answer : a. often contain genes for antibiotic resistance.
Description : Which one of the following vectors is used to replace the defective gene in gene therapy? (a) Adenovirus (b) Cosmid (c) Ri plasmid (d) Ti plasmid
Last Answer : (a) Adenovirus
Description : The process of replication in plasmid DNA, other than initiation, is controlled by (a) mitochondrial gene (b) plasmid gene (c) bacterial gene (d) none of these.
Last Answer : c) bacterial gene
Description : In genetic engineering, a DNA segment (gene) of interest, is transferred to the host cell through a vector. Consider the following four agents (i-iv) in this regard and select the correct option about which one or more of these ... ii) and (iv) (b) (i) only (c) (i) and (iii) (d) (ii) and (iv)
Last Answer : (d) (ii) and (iv)
Description : A gene whose expression helps to identify transformed cell is known as (a) vector (b) plasmid (c) structural gene (d) selectable marker.
Last Answer : (d) selectable marker.
Description : n bacteria, plasmid is (a) extra chromosomal material (b) main DNA (c) non functional DNA (d) repetitive gene.
Last Answer : (a) extra chromosomal material
Description : In bacteria, plasmid is (a) extra chromosomal material (b) main DNA (c) non functional DNA (d) repetitive gene.
Description : Which of the following defines an open reading frame (ORF)? A.A sequence of genome in bacteriophage B.The sequence of a complete genome C.A plasmid vector used in genome sequencing D.A possible gene predicted by DNA sequencing
Last Answer : D.A possible gene predicted by DNA sequencing
Description : Which of the following defines an open reading frame (ORF)? A- .A sequence of genome in bacteriophage B- The sequence of a complete genome C- A plasmid vector used in genome sequencing D- .A possible gene predicted by DNA sequencing
Last Answer : .A possible gene predicted by DNA sequencing
Description : When composite transposons are formed A- a small deletion occurs in the transposase gene of an IS element B- a small deletion occurs in the transposase gene of an IS element and plasmid is ... a plasmid D- two IS elements integrate into a chromosome with only a small distance separating them
Last Answer : two IS elements integrate into a chromosome with only a small distance separating them
Description : 7. In a recombinant DNA technology a plasmid vector must be cleaved by a) the same enzyme that leaves the donor gene b) modified DNA ligase c) a heated alkaline solution d) four separate enzymes
Last Answer : a) the same enzyme that leaves the donor gene
Description : A gene whose expression helps to identify transformed cell is known as : (1) Vector (2) Plasmid (3) Structural gene (4) Selectable marker
Last Answer : 4) Selectable marker
Description : Unidirectional tandem gene arrays are significant in ________ a. Increase in recombinant gene product with increasing plasmid number. b. Increase in recombinant gene product without increasing plasmid ... increasing plasmid numbers. d. Decrease in strong promotor with increase in plasmid number
Last Answer : b. Increase in recombinant gene product without increasing plasmid number.
Description : In pKO1 plasmid, galactose kinase gene is a reporter gene which lacks ___________ gene. a. Initiation codon b. Promoter c. Activator d. Termination
Last Answer : b. Promoter
Description : 8. The first r- DNA was constructed by linking antibiotic resistant gene with the native plasmid of a) Escherihia coli b) Salmonella typhimurium c) Acetobacter aceti d) Bacillus thuringiensis
Last Answer : Salmonella typhimurium
Description : The most important mechanism by which gram negative bacilli acquire chloramphenicol resistance is (a) Decreased permeability into the bacterial cell (b) Acquisition of a plasmid encoded ... bacterial ribosome for chloramphenicol (d) Switching over from ribosomal to mitochondrial protein synthesis
Description : A plasmid carries _____. a. Nonessential Genes b. Resistance Factors c. Genes For Toxins d. all the above
Last Answer : d. all the above
Description : The plasmid-mediated properties is/are A.fermentation of lactose B.production of enterotoxin C.resistance to antibiotics D.all of these
Last Answer : D.all of these
Description : The plasmid-mediated properties is/are A- fermentation of lactose B- production of enterotoxin C- resistance to antibiotics D- all of these
Last Answer : all of these
Description : The most important mechanism by which gram negative bacilli acquire chloramphenicol resistance is: A. Decreased permeability into the bacterial cell B. Acquisition of a plasmid encoded ... the bacterial ribosome for chloramphenicol D. Switching over from ribosomal to mitochondrial protein synthesi
Last Answer : B. Acquisition of a plasmid encoded for chloramphenicol acetyl transferas
Description : The most common mechanism of development of resistance to fluoroquinolones is: A. Chromosomal mutation altering affinity of target site B. Plasmid transfer C. Acquisition of drug destroying enzyme D. Acquisition of alternative metabolic pathway
Last Answer : A. Chromosomal mutation altering affinity of target site
Description : Antibiotic resistance genes in gm foods -Biology
Description : Source of antibiotic resistance genes for human pathogens. -Biology
Description : Where are antibiotic resistance genes located? -Biology
Description : Antibiotic resistance genes in Salmonella. -Biology
Description : Antibiotic resistance genes zone of inhibition -Biology
Description : What are the uses of plasmid? -Biology
Description : What is plasmid used for? -Biology
Description : Why do bacteria have plasmid? -Biology
Description : What is the origin of replication in a plasmid? -Biology
Description : Differences between a bacterial chromosome and a plasmid? -Biology
Description : Why does a plasmid need an origin of replication? -Biology
Description : What is plasmid DNA? -Biology
Description : Where is plasmid DNA found? -Biology
Description : Difference between plasmid and vector. -Biology
Description : Difference between plasmid and expression vector. -Biology