What is the principle of gel filtration chromatography?

What is the principle of gel filtration chromatography?
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The separation is effected on the basis of the size of the molecules. It is otherwise called molecular sieving.
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The sorting out of molecules according to size and shape may be adapted to protein purification in this technique: (A) Adsorption chromatography (B) Gel filtration chromatography (C) Paper chromatography (D) None of these

Description : The sorting out of molecules according to size and shape may be adapted to protein purification in this technique: (A) Adsorption chromatography (B) Gel filtration chromatography (C) Paper chromatography (D) None of these

Last Answer : Answer : B

The movement of charged particles towards one of the electrodes under the influence of electrical current is (A) Gel filtration (B) Molecular sieving (C) Gas liquid chromatography (D) Electrophoresis

Description : The movement of charged particles towards one of the electrodes under the influence of electrical current is (A) Gel filtration (B) Molecular sieving (C) Gas liquid chromatography (D) Electrophoresis

Last Answer : Answer : D

The technique for purification of proteins that can be made specific for a given protein is (A) Gel filtration chromotography (B) Ion exchange chromatography (C) Electrophoresis (D) Affinity chromatography

Description : The technique for purification of proteins that can be made specific for a given protein is (A) Gel filtration chromotography (B) Ion exchange chromatography (C) Electrophoresis (D) Affinity chromatography

Last Answer : Answer : D

Frequently employed materials for the adsorption chromatography of proteins include (A) High capacity supporting gel (B) Starch blocks (C) Calcium phosphate gel alumina gel and hydroxy apatite (D) All of these

Description : Frequently employed materials for the adsorption chromatography of proteins include (A) High capacity supporting gel (B) Starch blocks (C) Calcium phosphate gel alumina gel and hydroxy apatite (D) All of these

Last Answer : Answer : C

A process in which substances are separated throug differences in the rates at which the components migrate is called: w) filtration x) chromatography y) elution z) titration

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Last Answer : ANSWER: X -- CHROMATOGRAPHY

Give the principle of affinity chromatography.

Description : Give the principle of affinity chromatography. 

Last Answer : The technique is based on the high affinity of specific proteins for specific chemical groups.

What is the basic principle of ion-exchange chromatography?

Description : What is the basic principle of ion-exchange chromatography?

Last Answer : Here, the separation is based on electrostatic attraction between charged molecules to oppositely charged groups on the ion exchange resins.

What is the principle of partition chromatography?

Description : What is the principle of partition chromatography?

Last Answer : the components of the mixture to be separated are partitioned between the two phases depending on the partition co-efficient (solubility) of the particular substances.

What is the principle of adsorption chromatography?

Description :  What is the principle of adsorption chromatography? 

Last Answer : separation is based on differences in adsorption at the surface of a solid stationary medium.

Explain the principle of paper chromatography.

Description : Explain the principle of paper chromatography.

Last Answer : Paper chromatography is based on the difference in the rates at which the components of a mixture are adsorbed. The material on which different components are adsorbed is called ... compounds are adsorbed on stationary phase at different rates and separated by differential adsorption method.

Presence of arginine can be detected by (A) Sakaguchi reaction (B) Million-Nasse reaction (C) Hopkins-Cole reaction (D) Gas chromatography

Description : Presence of arginine can be detected by (A) Sakaguchi reaction (B) Million-Nasse reaction (C) Hopkins-Cole reaction (D) Gas chromatography

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The following technique makes use of the difference in net charges of proteins at a given pH: (A) Thin layer chromatography (B) Ion exchange chromatography (C) High performance liquid chromatography (D) Paper chromatography

Description : The following technique makes use of the difference in net charges of proteins at a given pH: (A) Thin layer chromatography (B) Ion exchange chromatography (C) High performance liquid chromatography (D) Paper chromatography

Last Answer : Answer : B

This technique takes the advantage of the fact that each protein has different pH at which it is electrically neutral i.e., its isoelectric pH: (A) Isoelectric focussing (B) Immunoel Ectro Phoresis (C) Chromatography (D) HPLC

Description : This technique takes the advantage of the fact that each protein has different pH at which it is electrically neutral i.e., its isoelectric pH: (A) Isoelectric focussing (B) Immunoel Ectro Phoresis (C) Chromatography (D) HPLC

Last Answer : Answer : A

A sharp moving boundary is obtained between the pure solvent and solute containing layer in (A) Chromatography (B) Immuno Reactivity (C) Ultra Centrifugation (D) Solubility curve

Description : A sharp moving boundary is obtained between the pure solvent and solute containing layer in (A) Chromatography (B) Immuno Reactivity (C) Ultra Centrifugation (D) Solubility curve

Last Answer : Answer :C

The amino acid which gives yellow colour with Ninhydrin in paper chromatography is (A) Tyrosine (B) Proline (C) Tryptophan (D) Alanine

Description : The amino acid which gives yellow colour with Ninhydrin in paper chromatography is (A) Tyrosine (B) Proline (C) Tryptophan (D) Alanine

Last Answer : Answer : B

Primary structure of proteins can be determined by the use of (A) Electrophoresis (B) Chromatography (C) Ninhydrin (D) Sanger’s reagent

Description : Primary structure of proteins can be determined by the use of (A) Electrophoresis (B) Chromatography (C) Ninhydrin (D) Sanger’s reagent

Last Answer : Answer : D

Give an example of affinity chromatography.

Description : Give an example of affinity chromatography.

Last Answer : Separation and quantitation of glycated hemoglobin.

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Description : What is the advantage of TLC over paper chromatography? 

Last Answer : TLC needs lesser time, and separation is more effective.

What are the common types of partition chromatography?

Description : What are the common types of partition chromatography?

Last Answer : Paper chromatography and thin layer chromatography.

What is the principle behind sterility testing? Describe Membrane filtration method for sterility testing.

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Last Answer : Principle: Basic principle of sterility test is that if bacteria or fungi are placed in a medium which provides the nutritive material and water and kept at favourable temperature the organism will grow and their presence can be indicated by the turbidity in the clear medium.

What is meant by chromatography? -Chemistry 9th

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Example of locating agents chromatography such as?

Description : Example of locating agents chromatography such as?

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What is Gas Chromatography ( GC) ?

Description : What is Gas Chromatography ( GC) ?

Last Answer : : Gas chromatography ( GC) is a method of identifying and quantifying the components of a mixture. This is done on a commercial gas chromatograph instrument. In gas chromatography a carrier gas ... time and the peak area of the detector signal are used to identify and quantify different components.

What is Chromatography ?

Description : What is Chromatography ?

Last Answer : : Chromatography is a method of separating the components of a mixture based on the absorption level or distribution coefficient by placing a mixture in a stationary medium and moving another moving medium in contact with that stationary medium.

What is meant by gas-liquid chromatography ?

Description : What is meant by gas-liquid chromatography ?

Last Answer : Gas-liquid chromatography is a type of partition chromatography , where the moving phase is an inert gas and the stationary phase is a liquid with a high boiling point absorbed on a solid surface. The ... . The components are dissolved due to the splitting of vapor , gas and liquid in the sample .

What is Gas Chromatography ( GC) ?

Description : What is Gas Chromatography ( GC) ?

Last Answer : : Gas chromatography ( GC) is a method of identifying and quantifying the components of a mixture. This is done on a commercial gas chromatograph instrument. In gas chromatography a carrier gas ... time and the peak area of the detector signal are used to identify and quantify different components.

Chromatography What ?

Last Answer : Chromatography Being Scientific Procedure _ _ A The process , of which Through Compound Or Mixed Of matter To the elements Separate To do Is. That Methodically A Carrier Moving To the ... Different Level Divided By Individual , identifiable, Amount Diagnosis To do Is Him Chromatography Says.

Two compounds I and II are eluted by column chromatography (adsorption of `I gt II`). Which one of the following is a correct statement ?

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$ Process of separation of photosynthetic pigments is called chromatography ! Chromatophore have accessory photosynthetic pigments such as phycocyanin

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What is a locating agent in chromatography?

Description : What is a locating agent in chromatography?

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Why is ethanol used in chromatography?

Description : Why is ethanol used in chromatography?

Last Answer : What is the answer ?

What is adsorption in chromatography?

Description : What is adsorption in chromatography?

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Radioactive elements can be traced out in living cell through a technique called – (1) Centrifugation (2) Chromatography (3) Auto radiography (4) All the above

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Give principles behind – i) Crystallization ii) Chromatography

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The compound of silicon used in chromatography is

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Fat emboli are best demonstrated in the lungs by C A. PCR B. Chromatography C. Frozen section examination of tissues stained with Sudan red D. None of these

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Organelles can be separated from cell homogenate through (a) chromatography (b) X-rays diffraction (c) differential centrifugation (d) auto-radiography

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Which of the following methods is used for characterization of impurities? A. TLC B. Mass spectroscopy C. Column chromatography D. Potentiometry

Description : Which of the following methods is used for characterization of impurities? A. TLC B. Mass spectroscopy C. Column chromatography D. Potentiometry

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Isolation of cellular components to determine their chemical composition is called (A) Cell differentiation (B) Chromatography (C) Cell fractionation (D) All of these

Description : Isolation of cellular components to determine their chemical composition is called (A) Cell differentiation (B) Chromatography (C) Cell fractionation (D) All of these

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HPLC is an abbreviation for? A. High Profit Liquid Chromatography B. High Pressure Liquid Chromatography C. Higher Performance Low Chromatography D. Higher Profit Low Chromatography

Description : HPLC is an abbreviation for? A. High Profit Liquid Chromatography B. High Pressure Liquid Chromatography C. Higher Performance Low Chromatography D. Higher Profit Low Chromatography

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The basis of the technique of chromatography for separating components of a mixture is? A. the differing movement of particles of different mass in an electrical field B. the interaction of the components with a stationary and a mobile phases C. the absorption of infrared radiation by the components. D. the deflection of charged particles in a magnetic field.

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Which of the following is used as a carrier gas in gas chromatography A. Carbon dioxide B. Oxygen C. Helium D. Methane

Description : Which of the following is used as a carrier gas in gas chromatography A. Carbon dioxide B. Oxygen C. Helium D. Methane

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Chromatography can be used to A. form mixtures B. change mixture compositions C. separate mixtures into pure substances D. all of these

Description : Chromatography can be used to A. form mixtures B. change mixture compositions C. separate mixtures into pure substances D. all of these

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What is the literal meaning of the word "chromatography"?

Description : What is the literal meaning of the word "chromatography"?

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Radioactive elements can be traced out in living cell through a technique called (1) Centrifugation (2) Chromatography (3) Auto radiography (4) All the above

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Which of the following techniques is used to separate proteins based upon differences in their mass? (A) Isoelectric focusing (B) Dialysis (C) SDS-gel Electrophoresis (D) Western blotting

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What is the advantage of polyacrylamide gel?

Description : What is the advantage of polyacrylamide gel?

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The blood sugar raising action of the hormone of suprarenal cortex is due to (A) Glyconeogenesis (B) Glycogenolysis (C) Glucagon like activity (D) due to inhibition of glomerular filtration of glucose

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Filtration fraction is increased in (A) Acute glomerulonephritis (B) Chronic glomerulonephritis (C) Hypertension (D) Hypotension

Description : Filtration fraction is increased in (A) Acute glomerulonephritis (B) Chronic glomerulonephritis (C) Hypertension (D) Hypotension

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