What is Gas Chromatography ( GC) ?

1 Answer

Answer :

: Gas chromatography ( GC) is a method of identifying and quantifying the components of a mixture. This is done on a commercial gas chromatograph instrument. In gas chromatography a carrier gas (usually inert gas or N2) is used as the mobile phase and a column as the stationary phase. The substance to be analyzed is taken in solution and injected into the head of the column with a microsyring. As a result, the solid overhead of the column is overlaid with different dimensions according to the structure of the object. A carrier gas ( He or N2) is then driven through it. The carrier will carry the gas object from the solution and pull it through the column. In this way different objects come out of the column at different speeds which are caught in the form of signal in the detector. The time it takes for each element to reach the emission mouth is called retention time . The capture time and the peak area of the detector signal are used to identify and quantify different components.

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Description : What is Gas Chromatography ( GC) ?

Last Answer : : Gas chromatography ( GC) is a method of identifying and quantifying the components of a mixture. This is done on a commercial gas chromatograph instrument. In gas chromatography a carrier gas ... time and the peak area of the detector signal are used to identify and quantify different components.

Description : What is meant by gas-liquid chromatography ?

Last Answer : Gas-liquid chromatography is a type of partition chromatography , where the moving phase is an inert gas and the stationary phase is a liquid with a high boiling point absorbed on a solid surface. The ... . The components are dissolved due to the splitting of vapor , gas and liquid in the sample .

Description : Why is the GameCube commonly abbreviated as "GCN," as opposed to just "GC"?

Last Answer : answer:Well it's abbreviated NGC, not GCN At least where I've seen it used. NGC - Nintendo Game Cube Now why do they say NGC instead of GC? I'm not sure, other than for clarification. There might ... that is abbreviated GC, whether it be a console or a game. Sorry I can't help anymore than that.

Description : About 2-3 hp, power per gallon of a thin liquid provides vigorous agitation in an agitator. 'Power number' in agitation is given by (A) P. gc /n 3 . D2 . ρ (B) P. gc . ρ/µ

Last Answer : (A) P. gc /n 3 . D2 . ρ

Description : In DNA, when AGCT occurs, their association is as per which of the following pair? (a) AT-GC (b) AG-CT (c) AC-GT (d) All of these

Last Answer : (a) AT-GC

Description : In frictional fluid flow, the quantity, (P/ρ) + (V 2 /2gc) + gz/gc) is (A) Constant along a streamline (B) Not constant along a streamline (C) Increased in the direction of flow (D) None of these

Last Answer : (B) Not constant along a streamline

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Last Answer : (D) With increase in the Mach number >0.6, the drag co-efficient decreases in case of compressible fluids

Description : A 600kg hammer of a pile driver is lilted 2m the pilling head. What is the change of potential energy? If the hammer is realest. What will be its velocity and the instant if it sticks the pilling?  a. 10,772 N-m and 5.26m/s ...  c. 11,772 N-m and 6.26m/s  d. 11,77 2N-m and5.26m/s ∫PE = mgo(∫Z)/gc

Last Answer : 11,772 N-m and 6.26m/s

Description : Presence of arginine can be detected by (A) Sakaguchi reaction (B) Million-Nasse reaction (C) Hopkins-Cole reaction (D) Gas chromatography

Last Answer : Answer : A

Description : The movement of charged particles towards one of the electrodes under the influence of electrical current is (A) Gel filtration (B) Molecular sieving (C) Gas liquid chromatography (D) Electrophoresis

Last Answer : Answer : D

Description : In Gas-Liquid Chromatography, some of the samples need to be derivatized in order to increase their (A) volatility (B) solubility (C)thermal conductivity (D) polarizability

Last Answer : (D) polarizability

Description : Which of the following is used as a carrier gas in gas chromatography A. Carbon dioxide B. Oxygen C. Helium D. Methane

Last Answer : Helium

Description : What is Chromatography ?

Last Answer : : Chromatography is a method of separating the components of a mixture based on the absorption level or distribution coefficient by placing a mixture in a stationary medium and moving another moving medium in contact with that stationary medium.

Description : Give principles behind – i) Crystallization ii) Chromatography

Last Answer : i) impure solid is dissolved in minimum amount of solvent, insoluble impurities are filtered out .solution containing soluble impurities is heated till crystallization point. The crystals of pure substance separate ... is solid the basis is adsorption and when it is liquid the basis is partition.

Description : Explain the principle of paper chromatography.

Last Answer : Paper chromatography is based on the difference in the rates at which the components of a mixture are adsorbed. The material on which different components are adsorbed is called ... compounds are adsorbed on stationary phase at different rates and separated by differential adsorption method.

Description : What is the literal meaning of the word "chromatography"?

Last Answer : ANSWER: COLOR-WRITING (or equivalent terminology)  

Description : A process in which substances are separated throug differences in the rates at which the components migrate is called: w) filtration x) chromatography y) elution z) titration

Last Answer : ANSWER: X -- CHROMATOGRAPHY

Description : What is meant by chromatography? -Chemistry 9th

Last Answer : Chromatography is a process for separating components of a mixture. ... The different components of the mixture travel through the stationary phase at different speeds, causing them to separate from one another

Description : Example of locating agents chromatography such as?

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Description : Two compounds I and II are eluted by column chromatography (adsorption of `I gt II`). Which one of the following is a correct statement ?

Last Answer : Two compounds I and II are eluted by column chromatography (adsorption of `I gt II`). Which one of the ... slower and has higher `R_(f)` value than I

Description : $ Process of separation of photosynthetic pigments is called chromatography ! Chromatophore have accessory photosynthetic pigments such as phycocyanin

Last Answer : $ Process of separation of photosynthetic pigments is called chromatography ! Chromatophore have accessory ... If both As and R are wrong.

Description : What is a locating agent in chromatography?

Last Answer : It is a chemical which reveals invisible spots on thechromatogram, e.g. ninhydrin for amino acids.

Description : Why is ethanol used in chromatography?

Last Answer : What is the answer ?

Description : What is adsorption in chromatography?

Last Answer : Adsorption chromatography (AC) is chromatographic separation due to energetic interactions between the solute and the surface of the porous packings.

Description : Frequently employed materials for the adsorption chromatography of proteins include (A) High capacity supporting gel (B) Starch blocks (C) Calcium phosphate gel alumina gel and hydroxy apatite (D) All of these

Last Answer : Answer : C

Description : The sorting out of molecules according to size and shape may be adapted to protein purification in this technique: (A) Adsorption chromatography (B) Gel filtration chromatography (C) Paper chromatography (D) None of these

Last Answer : Answer : B

Description : The following technique makes use of the difference in net charges of proteins at a given pH: (A) Thin layer chromatography (B) Ion exchange chromatography (C) High performance liquid chromatography (D) Paper chromatography

Last Answer : Answer : B

Description : This technique takes the advantage of the fact that each protein has different pH at which it is electrically neutral i.e., its isoelectric pH: (A) Isoelectric focussing (B) Immunoel Ectro Phoresis (C) Chromatography (D) HPLC

Last Answer : Answer : A

Description : A sharp moving boundary is obtained between the pure solvent and solute containing layer in (A) Chromatography (B) Immuno Reactivity (C) Ultra Centrifugation (D) Solubility curve

Last Answer : Answer :C

Description : The amino acid which gives yellow colour with Ninhydrin in paper chromatography is (A) Tyrosine (B) Proline (C) Tryptophan (D) Alanine

Last Answer : Answer : B

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Last Answer : Answer : D

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Last Answer : Answer : D

Description : Radioactive elements can be traced out in living cell through a technique called – (1) Centrifugation (2) Chromatography (3) Auto radiography (4) All the above

Last Answer : (3) Auto radiography Explanation: Radioactive elements can be traced out in living cell through a technique called Autoradiography.

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Last Answer : Separation and quantitation of glycated hemoglobin.

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Last Answer : The technique is based on the high affinity of specific proteins for specific chemical groups.

Description : What is the principle of gel filtration chromatography?

Last Answer : The separation is effected on the basis of the size of the molecules. It is otherwise called molecular sieving.

Description : What is the basic principle of ion-exchange chromatography?

Last Answer : Here, the separation is based on electrostatic attraction between charged molecules to oppositely charged groups on the ion exchange resins.

Description : What is the advantage of TLC over paper chromatography? 

Last Answer : TLC needs lesser time, and separation is more effective.

Description : What are the common types of partition chromatography?

Last Answer : Paper chromatography and thin layer chromatography.

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Description :  What is the principle of adsorption chromatography? 

Last Answer : separation is based on differences in adsorption at the surface of a solid stationary medium.

Description : The compound of silicon used in chromatography is

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