Description : In addition to DNA polymerase and primers what else does a polymerase chain require?
Last Answer : Need answer
Description : Template-directed DNA synthesis occurs in all the following except (A) The replication fork (B) Polymerase chain reaction (C) Growth of RNA tumor viruses (D) Expression of oncogenes
Last Answer : Answer : C
Description : Template-directed DNA synthesis occurs in all the following except (A) The replication fork (B) Polymerase chain reaction (C) Growth of RNA tumor viruses (D) Expression of oneogenes
Description : The figure below shows three steps (A, B, C) of Polymerase Chain Reaction (PCR). Select the option giving correct identification together with what it represents? A. B. C ds DNA 3 5 5 3 ... - extension in the presence of heat stable DNA polymerase (d) A - annealing with two sets of primers
Last Answer : (c) C - extension in the presence of heat stable DNA polymerase
Description : The correct statement concerning RNA and DNA polymerases is (A) RNA polymerase use nucleoside diphosphates (B) RNA polymerase require primers and add bases at 5' end of the growing polynucleotide ... and DNA polymerases can add nucleotides only at the 3' end of the growing polynucleotide chain
Last Answer : Answer : D
Description : The enzyme used in polymerase chain reaction (PCR) is (A) Taq polymerase (B) RNA polymerase (C) Ribonuclease (D) Endonuclease
Description : In the process of transcription in bacterial cells (A) Initiation requires rho protein (B) RNA polymerase incorporates methylated bases in correct sequence (C) Both the sigma unit and core ... RNA polymerase are required for accurate promotor site binding (D) Primase is necessary for initiation
Description : All of the following statements about RNA-dependent DNA polymerase are true except: (A) It synthesizes DNA using RNA as a template (B) It is also known as reverse transcriptase (C) It synthesizes DNA in 5’→3’ direction (D) It is present in all the viruses
Description : Primase activity is present in (A) DNA polymerase II (B) DNA polymerase α (C) DNA polymerase β (D) DNA polymerase δ
Last Answer : Answer : B
Description : RNA primer is formed by the enzyme: (A) Ribonuclease (B) Primase (C) DNA polymerase I (D) DNA polymerase III
Description : A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using (a) EcoRI (b) Taq polymerase (c) polymerase III (d) ligase.
Last Answer : (d) ligase.
Description : 9. . Which of the following steps are catalysed by Taq polymerase in a PCR reaction? (a) Denaturation of template DNA (b) Annealing of primers to template DNA (c) Extension of primer end on the template DNA (d) All of the above
Last Answer : (c) Extension of primer end on the template DNA
Description : The enzyme which catalyzes the unwinding of DNA helix during replication is: (1) Primase (2) Topoisomerase (3) Helicase (4) DNA polymerase
Last Answer : (3) Helicase Explanation: A type of helicase enzyme called DNA helicase catalyzes the unwinding of the DNA helix at the time of replication. It uses the energy released through hydrolysis of ATP ( ... The enzyme is needed to separate the two strands of DNA also during transcription and DNA repair.
Description : Which of the following enzymes are used to join bits of DNA? (a) Ligase (b) Primase (c) DNA polymerase (d) Endonuclease
Last Answer : a) Ligase
Description : The DNA polymerase commonly used in polymerase chain reaction is obtained from (A) E. coli (B) Yeast (C) T.aquaticus (D) Eukaryotes
Description : Polymerase chain reaction can rapidly amplify DNA sequences of the size (A) Upto 10 kilobases (B) Upto 45 kilobases (C) Upto 100 kilobases(D) Upto 1,000 kilobases
Last Answer : Answer : A
Description : In sanger’s method of DNA sequence determination, DNA synthesis is stopped by using (A) 1′, 2′- Dideoxyribonucleoside triphosphates (B) 2′, 3′- Dideoxyribonucleoside triphosphates (C) 2′, 4′- Dideoxyribonucleoside triphosphates (D) 2′, 5′ - Dideoxyribonucleoside triphosphates
Description : Why is Taq polymerase used in PCR? -Biology
Last Answer : Taq DNA Polymerase is highly efficient, so it becomes fully functional as it reaches its optimum temperature. It also has a half-life of more than two hours (at a temperature of 92 °C), a high-amplification capacity, and the ability to add 150 nucleotides per second.
Description : The Taq polymerase enzyme is obtained from (a) Bacillus subtilis (b) Pseudomonas putida (c) Thermus aquaticus (d) Thiobacillus ferroxidans.
Last Answer : (c) Thermus aquaticus
Description : RNA synthesis requires (A) RNA primer (B) RNA template (C) DNA template (D) DNA primer
Description : DNA-dependent RNA polymerase catalyses transcription on one strand of the DNA which is called the (a) template strand (b) coding strand (c) alpha strand (d) antistrand
Last Answer : (a) template strand
Description : DNA-dependent RNA polymerase catalyses transcription on one strand of the DNA which is called the (a) template strand (b) coding strand (c) alpha strand (d) antistrand.
Description : Which one of the following makes use of RNA template to synthesise DNA? (a) DNA polymerase (b) RNA polymerase (c) Reverse transcriptase (d) DNA dependant RNA polymerase
Last Answer : c) Reverse transcriptase
Description : The enzyme adds complementary bases to the DNA template strand during replication. a. Ligase b. Helicase c. DNA polymerase III d. RNA polymerase
Last Answer : d. RNA polymerase
Description : Which of the following reunites the exon segments after RNA splicing? (a) RNA polymerase (b) RNA primase (c) RNA ligase (d) RNA proteose
Last Answer : a) RNA polymerase
Description : Which of the following reunites the exon segments after RNA splicing? (a) RNA polymerase (b) RNA primase (c) RNA ligase (d) RNA proteoses
Last Answer : (c) RNA ligase
Description : The removal of which enzyme affects the synthesis of hnRNA in eukaryotes a) RNA polymerase II b) RNA primase c) RNA polymerase III d) RNA polymerase I
Last Answer : a) RNA polymerase II
Description : DNA po lymerase of T. aquat icus is preferred to that of E. coli in PCR because (A) It replicates DNA more efficiently (B) It doesn’t require primers (C) It is not denatured at the melting temperature of DNA (D) It doesn’t cause errors in replication
Description : The enzyme DNA ligase (A) Introduces superhelical twists (B) Connects the end of two DNA chains (C) Unwinds the double helix (D) Synthesises RNA primers
Description : DNA-dependent RNA polymerase requires the following for its catalytic activity: (A) Mg++ (B) Mn++ (C) Both (A) and (B) (D) None of these
Description : A scientist wants to make a dna fingerprint and she used polymerase chain reaction and restriction enzymes what should her next step be?
Last Answer : use restriction enzymes is the right answer for apex :)
Description : The following statement(s) is/are true concerning newer detection methods of systemic infection. a. Enzyme-linked immunosorbent assay (ELISA) is a rapid immunologic assay used for ... detected using advanced molecular techniques include cytomegalovirus (CMV) and human immunodeficiency virus (HIV)
Last Answer : Answer: a, b, c, d Although the classic detection of infection based on clinical signs of infection and bacterial culture remain the most common clinical tools, increasing ... including CMV and HIV. Furthermore, preliminary investigations into possible detection of fungal pathogens are underway
Description : DNA fragments generated by the restriction endo- nucleases in a chemical reaction can be separated by (a) electrophoresis (b) restriction mapping (c) centrifugation (d) polymerase chain reaction.
Last Answer : (a) electrophoresis
Description : Which of the following is not required for any of the techniques of DNA fingerprinting available at present? (a) Restriction enzymes (b) DNA-DNA hybridisation (c) Polymerase chain reaction (d) Zinc finger analysis
Last Answer : (d) Zinc finger analysis
Description : Deoxyribonucleotides are formed by reduction of (A) Ribonucleosides (B) Ribonucleoside monophosphates (C) Ribonucleoside diphosphates (D) Ribonucleoside triphosphates
Description : Which of the following statement is false (a). Enzymes are differentiated by electrophoresis method (b). While DNA sequencing both forward and reverse primers are used (c). Amplification is done through PCR (d). ABO blood grouping is mainly used for differentiating individuals
Last Answer : Ans: (b). While DNA sequencing both forward and reverse primers are used
Description : The following are true about DNA synthesis: a. it requires DNA polymerase b. reverse transcriptase enzymes are involved c. moves in a 5'---> 3' direction d. the rate of error in DNA synthesis is 1 in 105 base pairs
Last Answer : it requires DNA polymerase
Description : The α-amino group of the new amino acyl tRNA in the A site carries out a nucleophilic attack on the esterified carboxyl group of the peptidyl tRNA occupying the P site. This reaction is catalysed by (A) DNA polymerase (B) RNA polymerase (C) Peptidyl transferase (D) DNA ligase
Description : What is the role of the enzyme DNA primase?
Last Answer : Primase is an enzyme that synthesizes short RNA sequences called primers which serve as a starting point for DNA synthesis. Since primase produces RNA molecules, the enzyme is a type of RNA ... strand of nucleotides. Therefore, primase serves to prime and lay a foundation for DNA synthesis.
Description : Recombinant DNA is achieved by cleaving the pro-DNAs by (a) ligase (b) restriction endonuclease (c) primase (d) exonucleases.
Last Answer : (b) restriction endonuclease
Description : Manipulation of DNA in genetic engineering became possible due to the discovery of (a) restriction endonuclease (b) DNA ligase (c) transcriptase (d) primase
Last Answer : (a) restriction endonuclease
Description : Enzyme which can break and seal the DNA strand a) Topoisomease II (b) Helicase (c) Primase (d) Restriction endonuclease
Last Answer : a) Topoisomease II
Description : The enzyme which catalyzes the unwinding of DNA helix during replication is: (1) Primase (2) Topoisomerase (3) Helicase (4) DNApolymerase
Last Answer : C option is the answer
Description : What are the requirements for PCR (Polymerase Chain Reaction)? -Biology
Last Answer : answer:
Description : Steps involved in PCR (Polymerase Chain Reaction) process. -Biology
Last Answer : Step 1 DNA isolation.Step 2 Primer design.Step 3 Enzyme selection.Step 4 Thermal cycling.Step 5 Amplicon analysis.
Description : Why is polymerase chain reaction important? -Biology
Last Answer : Polymerase chain reaction (PCR) is often considered as one of the most important scientific advances in the field of molecular biology. With this revolutionary yet inexpensive biochemical technology, it's possible to generate millions of DNA copies from a single strand of DNA.
Description : What does polymerase chain reaction (pcr) do?
Last Answer : Polymerase chain reaction, or PCR, is a laboratory technique used to make multiple copies of a segment of DNA. PCR is very precise and can be used to amplify, or copy, a specific DNA target from a mixture of DNA molecules.