DNA fragments upto 45 kilobases in size can be cloned in (A) Bacterial plasmids (B) Lambda phage (C) Cosmids (D) Yeast artificial chromosomes

DNA fragments upto 45 kilobases in size can be cloned in (A) Bacterial plasmids (B) Lambda phage (C) Cosmids (D) Yeast artificial chromosomes
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Lambda phage can be used to clone DNA fragments of the size (A) Upto 3 kilobases (B) Upto 20 kilobases (C) Upto 45 kilobases (D) Upto 1,000 kilobases

Description : Lambda phage can be used to clone DNA fragments of the size (A) Upto 3 kilobases (B) Upto 20 kilobases (C) Upto 45 kilobases (D) Upto 1,000 kilobases

Last Answer : Answer : B

Polymerase chain reaction can rapidly amplify DNA sequences of the size (A) Upto 10 kilobases (B) Upto 45 kilobases (C) Upto 100 kilobases(D) Upto 1,000 kilobases

Description : Polymerase chain reaction can rapidly amplify DNA sequences of the size (A) Upto 10 kilobases (B) Upto 45 kilobases (C) Upto 100 kilobases(D) Upto 1,000 kilobases

Last Answer : Answer : A

Yeast artificial chromosome can be used to amplify DNA sequences of the size (A) Upto 10 kb (B) Upto 45 kb (C) Upto 100 kb (D) Upto 1,000 kb

Description : Yeast artificial chromosome can be used to amplify DNA sequences of the size (A) Upto 10 kb (B) Upto 45 kb (C) Upto 100 kb (D) Upto 1,000 kb

Last Answer : Answer : D

During the process of lysogeny _____. a. Phage DNA Integrates Into The Bacterial Chromosome.b. A Bacterium Acquires DNA From The External Environment. c. Competent Cells Receive Plasmids. d. New Phage Particles Are Assembled In The Host Bacterium.

Description : During the process of lysogeny _____. a. Phage DNA Integrates Into The Bacterial Chromosome. b. A Bacterium Acquires DNA From The External Environment. c. Competent Cells Receive Plasmids. d. New Phage Particles Are Assembled In The Host Bacterium.

Last Answer : a. Phage DNA Integrates Into The Bacterial Chromosome.

Competence refers to a bacterial cell's ability to a. Take Up DNA Fragments From The Environment. b. Be Infected By A Transducing Phage. c. Produce A Protein Product From An Engineered Gene. d. Undergo Conjugation With An F+ Cell.

Description : Competence refers to a bacterial cell's ability to a. Take Up DNA Fragments From The Environment. b. Be Infected By A Transducing Phage. c. Produce A Protein Product From An Engineered Gene. d. Undergo Conjugation With An F+ Cell.

Last Answer : a. Take Up DNA Fragments From The Environment.

A cosmid is a (A) Large bacterial plasmid (B) Viral plasmid (C) Hybrid of plasmid and phage (D) Yeast plasmid

Description : A cosmid is a (A) Large bacterial plasmid (B) Viral plasmid (C) Hybrid of plasmid and phage (D) Yeast plasmid

Last Answer : Answer : C

Transduction in bacteria is mediated by (a) plasmid vectors (b) phage vectors (c) cosmids (d) F-factors.

Description : Transduction in bacteria is mediated by (a) plasmid vectors (b) phage vectors (c) cosmids (d) F-factors.

Last Answer : (b) phage vectors

Which of the following may be used as a cloning vector? (A) Prokaryotic plasmid(B) Lambda phage (C) Cosmid (D) All of these

Description : Which of the following may be used as a cloning vector? (A) Prokaryotic plasmid(B) Lambda phage (C) Cosmid (D) All of these

Last Answer : Answer : D

Lac operon is a cluster of genes present in (A) Human beings (B) E. coli (C) Lambda phage (D) All of these

Description : Lac operon is a cluster of genes present in (A) Human beings (B) E. coli (C) Lambda phage (D) All of these

Last Answer : Answer : B

Which vector can clone only a small fragment of DNA? (a) Bacterial artificial chromosome (b) Yeast artificial chromosome (c) Plasmid (d) Cosmid

Description : Which vector can clone only a small fragment of DNA? (a) Bacterial artificial chromosome (b) Yeast artificial chromosome (c) Plasmid (d) Cosmid

Last Answer : (c) Plasmid

Which phage always carries a small piece of E.coli genome? A.Lambda B.Mu C.T4 D.T3

Description : Which phage always carries a small piece of E.coli genome? A.Lambda B.Mu C.T4 D.T3

Last Answer : B.Mu

Which phage always carries a small piece of E.coli genome? A- Lambda B- .Mu C- T4 D- T3

Description : Which phage always carries a small piece of E.coli genome? A- Lambda B- .Mu C- T4 D- T3

Last Answer : .Mu

Restriction endonucleases (A) Cut RNA chains at specific locations (B) Excise introns from hnRNA (C) Remove Okazaki fragments (D) Act as defensive enzymes to protect the host bacterial DNA from DNA of foreign organisms

Description : Restriction endonucleases (A) Cut RNA chains at specific locations (B) Excise introns from hnRNA (C) Remove Okazaki fragments (D) Act as defensive enzymes to protect the host bacterial DNA from DNA of foreign organisms

Last Answer : Answer : D

Which of the following statement describes plasmids? A- Another name for a protoplast B- A complex membrane structure that covers the chromosome of bacteria Small, circular DNA molecules that can exist independently of chromosomes commonly C- found in bacteria D- None of the above

Description : Which of the following statement describes plasmids? A- Another name for a protoplast B- A complex membrane structure that covers the chromosome of bacteria Small, circular DNA molecules that can exist independently of chromosomes commonly C- found in bacteria D- None of the above

Last Answer : found in bacteria

Which one of the following statements is true? a. Plasmids are circular molecules of RNA. b. RNA replicates by a conservative method of replication. c. Deletions or insertions of a nucleotide in a gene will cause a reading frameshift during translation. d. Okazaki fragments are removed from a RNA before translation occurs.

Description : Which one of the following statements is true? a. Plasmids are circular molecules of RNA. b. RNA replicates by a conservative method of replication. c. Deletions or insertions of a ... reading frameshift during translation. d. Okazaki fragments are removed from a RNA before translation occurs.

Last Answer : c. Deletions or insertions of a nucleotide in a gene will cause a reading frameshift during translation.

During the first 10 minutes after injection of phage DNA, no phage can be recovered by disrupting the infected bacterium. This is termed as A- eclipse period B- rise period C- latent period D- burst size

Description : During the first 10 minutes after injection of phage DNA, no phage can be recovered by disrupting the infected bacterium. This is termed as A- eclipse period B- rise period C- latent period D- burst size

Last Answer : eclipse period

Area of lysis on a bacterial lawn culture produced by a phage is known as A.pock B.plaque C.pox D.all of these

Description : Area of lysis on a bacterial lawn culture produced by a phage is known as A.pock B.plaque C.pox D.all of these

Last Answer : B.plaque

Area of lysis on a bacterial lawn culture produced by a phage is known as A.pock B.plaque C.pox D.all of these

Description : Area of lysis on a bacterial lawn culture produced by a phage is known as A.pock B.plaque C.pox D.all of these

Last Answer : .plaque

The first step in infection of a host bacterial cells by a phage is A- adsorption B- absorption C- penetration D- replication

Description : The first step in infection of a host bacterial cells by a phage is A- adsorption B- absorption C- penetration D- replication

Last Answer : adsorption

The promoter on an expression vector used to overproduce proteins in bacteria is A.a bacterial promoter B.expressed both in bacteria and mammalian cells C.not a regulated promoterD.the natural promoter of the gene being cloned

Description : The promoter on an expression vector used to overproduce proteins in bacteria is A.a bacterial promoter B.expressed both in bacteria and mammalian cells C.not a regulated promoter D.the natural promoter of the gene being cloned

Last Answer : A.a bacterial promoter

Plasmids are a. Another Name for Transposons. b. Accessory Genetic Information. c. Domains within A Chromosome. d. Daughter Chromosomes.

Description : Plasmids are a. Another Name for Transposons. b. Accessory Genetic Information. c. Domains within A Chromosome. d. Daughter Chromosomes.

Last Answer : b. Accessory Genetic Information.

Plasmids a. Replicate with the bacterial chromosome. b. Contain essential growth information. c. May contain antibiotic resistance genes. d. Are as large as the bacterial chromosome.

Description : Plasmids a. Replicate with the bacterial chromosome. b. Contain essential growth information. c. May contain antibiotic resistance genes. d. Are as large as the bacterial chromosome.

Last Answer : c. May contain antibiotic resistance genes.

4. Plasmids in bacterial cells are

Description : 5. 4. Plasmids in bacterial cells are (a) extra-chromosomal DNA, which cannot replicate (b) extra-chromosomal DNA, which can . self-replicate (c) extra DNA associated with the genome (d) extra DNA, associated with the genome, but cannot replicate.

Last Answer : (b) extra-chromosomal DNA, which can . self-replicate

Plasmids in bacterial cells are

Description : 4. . Plasmids in bacterial cells are (a) extra-chromosomal DNA, which cannot replicate (b) extra-chromosomal DNA, which can . self-replicate (c) extra DNA associated with the genome (d) extra DNA, associated with the genome, but cannot replicate.

Last Answer : (a) extra-chromosomal DNA, which cannot replicate

The fruit when kept is open, tastes bitter after 2 hours because of (A) Loss of water from juice (B) Decreased concentration of fructose in juice (C) Fermentation by yeast (D) Contamination by bacterial enzymes

Description : The fruit when kept is open, tastes bitter after 2 hours because of (A) Loss of water from juice (B) Decreased concentration of fructose in juice (C) Fermentation by yeast (D) Contamination by bacterial enzymes

Last Answer : Answer : D

$ DNA replication in a bacterial cell is bidirectional. ! A chromosomes with primary constriction is called "SAT" chromosomes.

Description : $ DNA replication in a bacterial cell is bidirectional. ! A chromosomes with primary constriction is called "SAT" chromosomes.

Last Answer : $ DNA replication in a bacterial cell is bidirectional. ! A chromosomes with primary constriction is called "SAT ... D. If both As and R are wrong.

Gel electrophoresis is used for (a) construction of recombinant DNA by joining with cloning vectors (b) isolation of DNA molecules (c) cutting of DNA into fragments (d) separation of DNA fragments according to their size.

Description : Gel electrophoresis is used for (a) construction of recombinant DNA by joining with cloning vectors (b) isolation of DNA molecules (c) cutting of DNA into fragments (d) separation of DNA fragments according to their size.

Last Answer : (d) separation of DNA fragments according to their size.

What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis ? (a) The smaller the fragment size, the farther it moves. (b) Positively charged fragments move to farther end. (c) Negatively charged fragments do not move. (d) The larger the fragment size, the farther it moves.

Description : What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis ? (a) The smaller the fragment size, the farther it moves. (b) Positively charged fragments move to farther end. ... charged fragments do not move. (d) The larger the fragment size, the farther it moves.

Last Answer : (a) The smaller the fragment size, the farther it moves

DNA fragments are (a) negatively charged (b) neutral (c) either positively or negatively charged depending on their size (d) positively charged

Description : DNA fragments are (a) negatively charged (b) neutral (c) either positively or negatively charged depending on their size (d) positively charged

Last Answer : (a) negatively charged

DNA fragments are: (1) Negatively charged (2) Neutral (3) Either positively or negatively charged depending on their size (4) Positively charged

Description : DNA fragments are: (1) Negatively charged (2) Neutral (3) Either positively or negatively charged depending on their size (4) Positively charged

Last Answer : (1) Negatively charged

The normal function of restriction endonucleases is to (A) Excise introns from hrRNA (B) Polymerize nucleotides to form RNA (C) Remove primer from okazaki fragments (D) Protect bacteria from foreign DNA

Description : The normal function of restriction endonucleases is to (A) Excise introns from hrRNA (B) Polymerize nucleotides to form RNA (C) Remove primer from okazaki fragments (D) Protect bacteria from foreign DNA

Last Answer : Answer : D

Okasaki fragments are small bits of (A) RNA (B) DNA (C) DNA with RNA heads (D) RNA with DNA heads

Description : Okasaki fragments are small bits of (A) RNA (B) DNA (C) DNA with RNA heads (D) RNA with DNA heads

Last Answer : Answer : C

Fragments of DNA can be identified by the technique of (A) Western blotting (B) Eastern blotting (C) Northern blotting (D) Southern blotting

Description : Fragments of DNA can be identified by the technique of (A) Western blotting (B) Eastern blotting (C) Northern blotting (D) Southern blotting

Last Answer : Answer : D

DNA fragments are sealed by (A) DNA polymerase II (B) DNA ligase (C) DNA gyrase (D) DNA topoisomerase II

Description : DNA fragments are sealed by (A) DNA polymerase II (B) DNA ligase (C) DNA gyrase (D) DNA topoisomerase II

Last Answer : Answer : B

The complement system consists of a series of serum proteins that exist in a quiescent or very low-level state of activation in the uninfected host. Which of the following statement(s) is/are true concerning complement activation? a. The alternate (properdin) pathway of complement activation can occur directly through contact with fungal or bacterial cell wall compounds b. Complement component fragments may serve to decrease vascular permeability c. Excessive complement activation can produce deleterious effects d. Fragments of certain complement components serve as chemoattractants to additional cellular components of the host defense mechanism

Description : The complement system consists of a series of serum proteins that exist in a quiescent or very low-level state of activation in the uninfected host. Which of the following ... certain complement components serve as chemoattractants to additional cellular components of the host defense mechanism

Last Answer : Answer: a, c, d Complement activation can occur through either classic or alternate (properdin) pathways, both of which eventuate in deposition of terminal complement pathway components on the ... and release of lysosomal enzymes that can directly damage certain target tissues, such as the lung

Pyrogens are a. Proteins Affecting the Hypothalamus. b. Bacterial Fragments. c. Fever-Producing Substances. d. All the Above (A—C) Are Correct.

Description : Pyrogens are a. Proteins Affecting the Hypothalamus. b. Bacterial Fragments. c. Fever-Producing Substances. d. All the Above (A—C) Are Correct.

Last Answer : d. All the Above (A—C) Are Correct.

Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes? (a) Retrovirus (b) Ti plasmid (c) λ phage (d) pBR322

Description : Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes? (a) Retrovirus (b) Ti plasmid (c) λ phage (d) pBR322

Last Answer : (a) Retrovirus

In the extracellular medium, DNA-degrading enzymes would likely be to prevent transfer of DNA by A- conjugal transfer by a self-transmissible plasmid B- generalized phage transduction C- natural transformation D- none of the above

Description : In the extracellular medium, DNA-degrading enzymes would likely be to prevent transfer of DNA by A- conjugal transfer by a self-transmissible plasmid B- generalized phage transduction C- natural transformation D- none of the above

Last Answer : natural transformation

Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes ? (1) Retrovirus (2) Ti plasmid (3)  phage (4) pBR 322

Description : Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes ? (1) Retrovirus (2) Ti plasmid (3)  phage (4) pBR 322

Last Answer : (1) Retrovirus

What are cosmids? -Biology

Description : What are cosmids? -Biology

Last Answer : answer:

What are cosmids? -Biology

Description : What are cosmids? -Biology

Last Answer : answer:

What are plasmids? What is the importance of plasmids for the recombinant DNA technology?

Description : What are plasmids? What is the importance of plasmids for the recombinant DNA technology?

Last Answer : Plasmids are circular fragments of DNA that are accessories to the main bacterial DNA. Plasmids are important for genetic engineering because genes from other organisms are inserted into them to ... utile proteins for humans on an industrial scale. Bacterial Cell Review - Image Diversity: plasmid

Without restriction endonucleases, it would be very difficult to a. Force Plasmids into Bacteria. b. Chemically Open Dna Molecules. c. Replicate Dna In A Recombinant Cell. d. Bring About Mutations In Bacteria.

Description : Without restriction endonucleases, it would be very difficult to a. Force Plasmids into Bacteria. b. Chemically Open Dna Molecules. c. Replicate Dna In A Recombinant Cell. d. Bring About Mutations In Bacteria.

Last Answer : b. Chemically Open Dna Molecules.

are single-stranded DNA molecules that can recognize and bind to a distinctive nucLeotide sequence of a pathogen. a. Prophages b. Plasmids c. Cloning vectors d. DNA probes

Description : are single-stranded DNA molecules that can recognize and bind to a distinctive nucLeotide sequence of a pathogen. a. Prophages b. Plasmids c. Cloning vectors d. DNA probes

Last Answer : d. DNA probes

The DNA polymerase commonly used in polymerase chain reaction is obtained from (A) E. coli (B) Yeast (C) T.aquaticus (D) Eukaryotes

Description : The DNA polymerase commonly used in polymerase chain reaction is obtained from (A) E. coli (B) Yeast (C) T.aquaticus (D) Eukaryotes

Last Answer : Answer : C

Human insulins are obtained by the following sources/methods except: A. Cadaver pancreas B. Proinsulin recombinant bacterial C. Precursor yeast recombinant D. Enzyme modification of **** insulin

Description : Human insulins are obtained by the following sources/methods except: A. Cadaver pancreas B. Proinsulin recombinant bacterial C. Precursor yeast recombinant D. Enzyme modification of pork insulin

Last Answer : . Cadaver pancreas

The most suitable pore size for bacterial filtration in a membrane filter is ------- A 0.22 mm B 0.45 nm C 0.22 µm D 30 µm

Description : The most suitable pore size for bacterial filtration in a membrane filter is ------- A 0.22 mm B 0.45 nm C 0.22 µm D 30 µm

Last Answer : C 0.22 µm

Light chains of immunoglobulins are of following types: (A) Alpha and kappa (B) Alpha and gamma (C) Lambda and delta(D) Kappa and lambda

Description : Light chains of immunoglobulins are of following types: (A) Alpha and kappa (B) Alpha and gamma (C) Lambda and delta(D) Kappa and lambda

Last Answer : Answer : D

What is the technique used for separation of DNA fragments. -Biology

Description : What is the technique used for separation of DNA fragments. -Biology

Last Answer : answer: