Lambda phage can be used to clone DNA fragments of the size (A) Upto 3 kilobases (B) Upto 20 kilobases (C) Upto 45 kilobases (D) Upto 1,000 kilobases

Lambda phage can be used to clone DNA fragments of the size (A) Upto 3 kilobases (B) Upto 20 kilobases (C) Upto 45 kilobases (D) Upto 1,000 kilobases
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DNA fragments upto 45 kilobases in size can be cloned in (A) Bacterial plasmids (B) Lambda phage (C) Cosmids (D) Yeast artificial chromosomes

Description : DNA fragments upto 45 kilobases in size can be cloned in (A) Bacterial plasmids (B) Lambda phage (C) Cosmids (D) Yeast artificial chromosomes

Last Answer : Answer : C

Polymerase chain reaction can rapidly amplify DNA sequences of the size (A) Upto 10 kilobases (B) Upto 45 kilobases (C) Upto 100 kilobases(D) Upto 1,000 kilobases

Description : Polymerase chain reaction can rapidly amplify DNA sequences of the size (A) Upto 10 kilobases (B) Upto 45 kilobases (C) Upto 100 kilobases(D) Upto 1,000 kilobases

Last Answer : Answer : A

Yeast artificial chromosome can be used to amplify DNA sequences of the size (A) Upto 10 kb (B) Upto 45 kb (C) Upto 100 kb (D) Upto 1,000 kb

Description : Yeast artificial chromosome can be used to amplify DNA sequences of the size (A) Upto 10 kb (B) Upto 45 kb (C) Upto 100 kb (D) Upto 1,000 kb

Last Answer : Answer : D

Which of the following may be used as a cloning vector? (A) Prokaryotic plasmid(B) Lambda phage (C) Cosmid (D) All of these

Description : Which of the following may be used as a cloning vector? (A) Prokaryotic plasmid(B) Lambda phage (C) Cosmid (D) All of these

Last Answer : Answer : D

Lac operon is a cluster of genes present in (A) Human beings (B) E. coli (C) Lambda phage (D) All of these

Description : Lac operon is a cluster of genes present in (A) Human beings (B) E. coli (C) Lambda phage (D) All of these

Last Answer : Answer : B

Competence refers to a bacterial cell's ability to a. Take Up DNA Fragments From The Environment. b. Be Infected By A Transducing Phage. c. Produce A Protein Product From An Engineered Gene. d. Undergo Conjugation With An F+ Cell.

Description : Competence refers to a bacterial cell's ability to a. Take Up DNA Fragments From The Environment. b. Be Infected By A Transducing Phage. c. Produce A Protein Product From An Engineered Gene. d. Undergo Conjugation With An F+ Cell.

Last Answer : a. Take Up DNA Fragments From The Environment.

Which phage always carries a small piece of E.coli genome? A.Lambda B.Mu C.T4 D.T3

Description : Which phage always carries a small piece of E.coli genome? A.Lambda B.Mu C.T4 D.T3

Last Answer : B.Mu

Which phage always carries a small piece of E.coli genome? A- Lambda B- .Mu C- T4 D- T3

Description : Which phage always carries a small piece of E.coli genome? A- Lambda B- .Mu C- T4 D- T3

Last Answer : .Mu

During the first 10 minutes after injection of phage DNA, no phage can be recovered by disrupting the infected bacterium. This is termed as A- eclipse period B- rise period C- latent period D- burst size

Description : During the first 10 minutes after injection of phage DNA, no phage can be recovered by disrupting the infected bacterium. This is termed as A- eclipse period B- rise period C- latent period D- burst size

Last Answer : eclipse period

Gel electrophoresis is used for (a) construction of recombinant DNA by joining with cloning vectors (b) isolation of DNA molecules (c) cutting of DNA into fragments (d) separation of DNA fragments according to their size.

Description : Gel electrophoresis is used for (a) construction of recombinant DNA by joining with cloning vectors (b) isolation of DNA molecules (c) cutting of DNA into fragments (d) separation of DNA fragments according to their size.

Last Answer : (d) separation of DNA fragments according to their size.

What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis ? (a) The smaller the fragment size, the farther it moves. (b) Positively charged fragments move to farther end. (c) Negatively charged fragments do not move. (d) The larger the fragment size, the farther it moves.

Description : What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis ? (a) The smaller the fragment size, the farther it moves. (b) Positively charged fragments move to farther end. ... charged fragments do not move. (d) The larger the fragment size, the farther it moves.

Last Answer : (a) The smaller the fragment size, the farther it moves

DNA fragments are (a) negatively charged (b) neutral (c) either positively or negatively charged depending on their size (d) positively charged

Description : DNA fragments are (a) negatively charged (b) neutral (c) either positively or negatively charged depending on their size (d) positively charged

Last Answer : (a) negatively charged

DNA fragments are: (1) Negatively charged (2) Neutral (3) Either positively or negatively charged depending on their size (4) Positively charged

Description : DNA fragments are: (1) Negatively charged (2) Neutral (3) Either positively or negatively charged depending on their size (4) Positively charged

Last Answer : (1) Negatively charged

The normal function of restriction endonucleases is to (A) Excise introns from hrRNA (B) Polymerize nucleotides to form RNA (C) Remove primer from okazaki fragments (D) Protect bacteria from foreign DNA

Description : The normal function of restriction endonucleases is to (A) Excise introns from hrRNA (B) Polymerize nucleotides to form RNA (C) Remove primer from okazaki fragments (D) Protect bacteria from foreign DNA

Last Answer : Answer : D

Okasaki fragments are small bits of (A) RNA (B) DNA (C) DNA with RNA heads (D) RNA with DNA heads

Description : Okasaki fragments are small bits of (A) RNA (B) DNA (C) DNA with RNA heads (D) RNA with DNA heads

Last Answer : Answer : C

Fragments of DNA can be identified by the technique of (A) Western blotting (B) Eastern blotting (C) Northern blotting (D) Southern blotting

Description : Fragments of DNA can be identified by the technique of (A) Western blotting (B) Eastern blotting (C) Northern blotting (D) Southern blotting

Last Answer : Answer : D

DNA fragments are sealed by (A) DNA polymerase II (B) DNA ligase (C) DNA gyrase (D) DNA topoisomerase II

Description : DNA fragments are sealed by (A) DNA polymerase II (B) DNA ligase (C) DNA gyrase (D) DNA topoisomerase II

Last Answer : Answer : B

Restriction endonucleases (A) Cut RNA chains at specific locations (B) Excise introns from hnRNA (C) Remove Okazaki fragments (D) Act as defensive enzymes to protect the host bacterial DNA from DNA of foreign organisms

Description : Restriction endonucleases (A) Cut RNA chains at specific locations (B) Excise introns from hnRNA (C) Remove Okazaki fragments (D) Act as defensive enzymes to protect the host bacterial DNA from DNA of foreign organisms

Last Answer : Answer : D

Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes? (a) Retrovirus (b) Ti plasmid (c) λ phage (d) pBR322

Description : Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes? (a) Retrovirus (b) Ti plasmid (c) λ phage (d) pBR322

Last Answer : (a) Retrovirus

During the process of lysogeny _____. a. Phage DNA Integrates Into The Bacterial Chromosome.b. A Bacterium Acquires DNA From The External Environment. c. Competent Cells Receive Plasmids. d. New Phage Particles Are Assembled In The Host Bacterium.

Description : During the process of lysogeny _____. a. Phage DNA Integrates Into The Bacterial Chromosome. b. A Bacterium Acquires DNA From The External Environment. c. Competent Cells Receive Plasmids. d. New Phage Particles Are Assembled In The Host Bacterium.

Last Answer : a. Phage DNA Integrates Into The Bacterial Chromosome.

In the extracellular medium, DNA-degrading enzymes would likely be to prevent transfer of DNA by A- conjugal transfer by a self-transmissible plasmid B- generalized phage transduction C- natural transformation D- none of the above

Description : In the extracellular medium, DNA-degrading enzymes would likely be to prevent transfer of DNA by A- conjugal transfer by a self-transmissible plasmid B- generalized phage transduction C- natural transformation D- none of the above

Last Answer : natural transformation

Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes ? (1) Retrovirus (2) Ti plasmid (3)  phage (4) pBR 322

Description : Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes ? (1) Retrovirus (2) Ti plasmid (3)  phage (4) pBR 322

Last Answer : (1) Retrovirus

The maximum spacing of manholes specified by Indian standard in sewers upto 0.3 m diameter is A. 20 m B. 30 m C. 45 m D. 75 m

Description : The maximum spacing of manholes specified by Indian standard in sewers upto 0.3 m diameter is A. 20 m B. 30 m C. 45 m D. 75 m

Last Answer : ANS: C

Dolomite is a lime stone which contains carbonate of magnesia upto (A) 15 % (B) 20 % (C) 25 % (D) 45 %

Description : Dolomite is a lime stone which contains carbonate of magnesia upto (A) 15 % (B) 20 % (C) 25 % (D) 45 %

Last Answer : Answer: Option D

An IP datagram of size 1000 bytes arrives at a router. The router has to forward this packet on a link whose MTU (maximum transmission unit) is 100 bytes. Assume that the size of the IP header is 20 bytes. The number of fragments that the IP datagram will be divided into for transmission is : a. 10 b. 50 c. 12 d. 13

Description : An IP datagram of size 1000 bytes arrives at a router. The router has to forward this packet on a link whose MTU (maximum transmission unit) is 100 bytes. Assume that the size of the IP header is 20 bytes ... the IP datagram will be divided into for transmission is : a. 10 b. 50 c. 12 d. 13

Last Answer : d. 13

$100,000 to clone my dog?

Description : $100,000 to clone my dog?

Last Answer : Even a clone wouldn’t guarantee the same temperament. Better to find out if Pitch has breeding relatives. College may not be the best investment but please don’t starve the cat. They have ways of reciprocating

A cosmid is a (A) Large bacterial plasmid (B) Viral plasmid (C) Hybrid of plasmid and phage (D) Yeast plasmid

Description : A cosmid is a (A) Large bacterial plasmid (B) Viral plasmid (C) Hybrid of plasmid and phage (D) Yeast plasmid

Last Answer : Answer : C

Light chains of immunoglobulins are of following types: (A) Alpha and kappa (B) Alpha and gamma (C) Lambda and delta(D) Kappa and lambda

Description : Light chains of immunoglobulins are of following types: (A) Alpha and kappa (B) Alpha and gamma (C) Lambda and delta(D) Kappa and lambda

Last Answer : Answer : D

DNA or RNA segment tagged with a radioactive molecule is called (a) vector (b) probe (c) clone (d) plasmid.

Description : DNA or RNA segment tagged with a radioactive molecule is called (a) vector (b) probe (c) clone (d) plasmid.

Last Answer : (b) probe

Which vector can clone only a small fragment of DNA? (a) Bacterial artificial chromosome (b) Yeast artificial chromosome (c) Plasmid (d) Cosmid

Description : Which vector can clone only a small fragment of DNA? (a) Bacterial artificial chromosome (b) Yeast artificial chromosome (c) Plasmid (d) Cosmid

Last Answer : (c) Plasmid

What is the technique used for separation of DNA fragments. -Biology

Description : What is the technique used for separation of DNA fragments. -Biology

Last Answer : answer:

How does gel electrophoresis separate DNA fragments?

Description : How does gel electrophoresis separate DNA fragments?

Last Answer : There is a specific density of gel used in the electrophoresis.The DNA is placed in a well, and then electrical charge is used topull the DNA through the gel. Because spliced DNA is ... taxi cab, which is larger, movesslower through the traffic. The bicycle which is smaller, movesquicker.

A scientist uses materials in her lab to cut a dna sample into fragments what process is the scientist using?

Description : A scientist uses materials in her lab to cut a dna sample into fragments what process is the scientist using?

Last Answer : PCR or polymerase chain reaction. :: Apex

Why can gel electrophoresis separate dna fragments?

Description : Why can gel electrophoresis separate dna fragments?

Last Answer : Electrophoresis enables you to distinguish DNA fragments of different lengths. DNA is negatively charged, therefore, when an electric current is applied to the gel, DNA will migrate towards the positively ... marker, you can work out the approximate length of the DNA fragments in the samples.

Why can gel electrophoresis separate dna fragments?

Description : Why can gel electrophoresis separate dna fragments?

Last Answer : Electrophoresis enables you to distinguish DNA fragments of different lengths. DNA is negatively charged, therefore, when an electric current is applied to the gel, DNA will migrate towards the positively ... marker, you can work out the approximate length of the DNA fragments in the samples.

On what basis the short fragments of DNA arrange themselves?

Description : On what basis the short fragments of DNA arrange themselves?

Last Answer : The short fragments of DNA get separated on the basis of their size and net electrical charge.

The restriction enzymes are used in genetic engineering, because (a) they can cut DNA at specific base sequence (b) they are nucleases that cut DNA at variable sites (c) they can degrade harmful proteins (d) they can join different DNA fragments.

Description : The restriction enzymes are used in genetic engineering, because (a) they can cut DNA at specific base sequence (b) they are nucleases that cut DNA at variable sites (c) they can degrade harmful proteins (d) they can join different DNA fragments.

Last Answer : (a) they can cut DNA at specific base sequence

DNA fragments generated by the restriction endo- nucleases in a chemical reaction can be separated by (a) electrophoresis (b) restriction mapping (c) centrifugation (d) polymerase chain reaction.

Description : DNA fragments generated by the restriction endo- nucleases in a chemical reaction can be separated by (a) electrophoresis (b) restriction mapping (c) centrifugation (d) polymerase chain reaction.

Last Answer : (a) electrophoresis

The DNA fragments separated on an agarose gel can be visualised after staining with (a) acetocarmine (b) aniline blue (c) ethidium bromide (d) bromophenol blue.

Description : The DNA fragments separated on an agarose gel can be visualised after staining with (a) acetocarmine (b) aniline blue (c) ethidium bromide (d) bromophenol blue.

Last Answer : (c) ethidium bromide

In gel electrophoresis, separated DNA fragments can be visualized with the help of (a) acetocarmine in bright blue light (b) ethidium bromide in UV radiation (c) acetocarmine in UV radiation (d) ethidium bromide in infrared radiation.

Description : In gel electrophoresis, separated DNA fragments can be visualized with the help of (a) acetocarmine in bright blue light (b) ethidium bromide in UV radiation (c) acetocarmine in UV radiation (d) ethidium bromide in infrared radiation.

Last Answer : (b) ethidium bromide in UV radiation

Choose the correct pair from the following. (a) Ligases - Join the two DNA molecules (b) Polymerases - Break the DNA into fragments (c) Nucleases - Separate the two strands of DNA (d) Exonucleases - Make cuts at specific positions within DNA

Description : Choose the correct pair from the following. (a) Ligases - Join the two DNA molecules (b) Polymerases - Break the DNA into fragments (c) Nucleases - Separate the two strands of DNA (d) Exonucleases - Make cuts at specific positions within DNA

Last Answer : (a) Ligases - Join the two DNA molecules

During DNA replication, Okazaki fragments are used to elongate (a) the lagging strand towards replication fork (b) the leading strand away from replication fork (c) the lagging strand away from the replication fork (d) the leading strand towards replication fork.

Description : During DNA replication, Okazaki fragments are used to elongate (a) the lagging strand towards replication fork (b) the leading strand away from replication fork (c) the lagging strand away from the replication fork (d) the leading strand towards replication fork.

Last Answer : (b) the leading strand away from replication fork

Transformation refers to a. Using A Virus To Transfer DNA Fragments. b. DNA Fragments Transferred Between Live Donor And Recipient Cells. c. The Formation of an F- Recombinant Cell. d. The Transfer of Naked Fragments of DNA

Description : Transformation refers to a. Using A Virus To Transfer DNA Fragments. b. DNA Fragments Transferred Between Live Donor And Recipient Cells. c. The Formation of an F- Recombinant Cell. d. The Transfer of Naked Fragments of DNA

Last Answer : b. DNA Fragments Transferred Between Live Donor And Recipient Cells.

In the semiconservative method of replication a. Both parent strands are degraded. b. One parent strand is conserved in each of the the new dna. c. Both parent strands reform with one another. d. Okazaki fragments form both of the new molecules of DNA.

Description : In the semiconservative method of replication a. Both parent strands are degraded. b. One parent strand is conserved in each of the the new dna. c. Both parent strands reform with one another. d. Okazaki fragments form both of the new molecules of DNA.

Last Answer : b. One parent strand is conserved in each of the the new dna.

At a chromosome replication fork, the lagging strand consists of__ That is joined by___. a. RNA sequences; DNA ligase b. Okazaki Fragments; RNA Polymerase c. RNA sequences; ribosomes d. Okazaki fragments; DNA ligase

Description : At a chromosome replication fork, the lagging strand consists of__ That is joined by___. a. RNA sequences; DNA ligase b. Okazaki Fragments; RNA Polymerase c. RNA sequences; ribosomes d. Okazaki fragments; DNA ligase

Last Answer : d. Okazaki fragments; DNA ligase

In DNA sequencing, the primer A.specifies where the sequence ends B.specifies where the sequence begins C.both (a) and (b) D.generates variety of different sized fragments

Description : In DNA sequencing, the primer A.specifies where the sequence ends B.specifies where the sequence begins C.both (a) and (b) D.generates variety of different sized fragments

Last Answer : B.specifies where the sequence begins

The DNA fragments produced by the use of restriction endonucleases can be separated by

Description : 4. The DNA fragments produced by the use of restriction endonucleases can be separated by (a) polymerase chain reaction (b) gel electrophoresis (c) density gradient centrifugation (d) any of the above.

Last Answer : gel electrophoresis

During DNA replication, Okazaki fragments are used to elongate: (1) The lagging strand towards replication fork. (2) The leading strand away from replication fork. (3) The lagging strand away from the replication fork. (4) The leading strand towards replication fork.

Description : During DNA replication, Okazaki fragments are used to elongate: (1) The lagging strand towards replication fork. (2) The leading strand away from replication fork. (3) The lagging strand away from the replication fork. (4) The leading strand towards replication fork.

Last Answer : (3) The lagging strand away from the replication fork.

The DNA fragments separated on an agarose gel can be visualised after staining with : (1) Acetocarmine (2) Aniline blue (3) Ethidium bromide (4) Bromophenol blue

Description : The DNA fragments separated on an agarose gel can be visualised after staining with : (1) Acetocarmine (2) Aniline blue (3) Ethidium bromide (4) Bromophenol blue

Last Answer : (3) Ethidium bromide