What is the technique used for separation of DNA fragments. -Biology

What is the technique used for separation of DNA fragments. -Biology
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Gel electrophoresis is used for (a) construction of recombinant DNA by joining with cloning vectors (b) isolation of DNA molecules (c) cutting of DNA into fragments (d) separation of DNA fragments according to their size.

Description : Gel electrophoresis is used for (a) construction of recombinant DNA by joining with cloning vectors (b) isolation of DNA molecules (c) cutting of DNA into fragments (d) separation of DNA fragments according to their size.

Last Answer : (d) separation of DNA fragments according to their size.

Fragments of DNA can be identified by the technique of (A) Western blotting (B) Eastern blotting (C) Northern blotting (D) Southern blotting

Description : Fragments of DNA can be identified by the technique of (A) Western blotting (B) Eastern blotting (C) Northern blotting (D) Southern blotting

Last Answer : Answer : D

Technique to implement virtual memory where memory is divided into units of fixed size memory is _________ A. Paging B. De-fragments C. Segmentation D. None of the above

Description : Technique to implement virtual memory where memory is divided into units of fixed size memory is _________ A. Paging B. De-fragments C. Segmentation D. None of the above

Last Answer : A. Paging

The technique of producing plants from minute fragments is called?

Description : The technique of producing plants from  minute fragments is called?

Last Answer : Tissue culture

How does gel electrophoresis separate DNA fragments?

Description : How does gel electrophoresis separate DNA fragments?

Last Answer : There is a specific density of gel used in the electrophoresis.The DNA is placed in a well, and then electrical charge is used topull the DNA through the gel. Because spliced DNA is ... taxi cab, which is larger, movesslower through the traffic. The bicycle which is smaller, movesquicker.

A scientist uses materials in her lab to cut a dna sample into fragments what process is the scientist using?

Description : A scientist uses materials in her lab to cut a dna sample into fragments what process is the scientist using?

Last Answer : PCR or polymerase chain reaction. :: Apex

Why can gel electrophoresis separate dna fragments?

Description : Why can gel electrophoresis separate dna fragments?

Last Answer : Electrophoresis enables you to distinguish DNA fragments of different lengths. DNA is negatively charged, therefore, when an electric current is applied to the gel, DNA will migrate towards the positively ... marker, you can work out the approximate length of the DNA fragments in the samples.

Why can gel electrophoresis separate dna fragments?

Description : Why can gel electrophoresis separate dna fragments?

Last Answer : Electrophoresis enables you to distinguish DNA fragments of different lengths. DNA is negatively charged, therefore, when an electric current is applied to the gel, DNA will migrate towards the positively ... marker, you can work out the approximate length of the DNA fragments in the samples.

The normal function of restriction endonucleases is to (A) Excise introns from hrRNA (B) Polymerize nucleotides to form RNA (C) Remove primer from okazaki fragments (D) Protect bacteria from foreign DNA

Description : The normal function of restriction endonucleases is to (A) Excise introns from hrRNA (B) Polymerize nucleotides to form RNA (C) Remove primer from okazaki fragments (D) Protect bacteria from foreign DNA

Last Answer : Answer : D

Okasaki fragments are small bits of (A) RNA (B) DNA (C) DNA with RNA heads (D) RNA with DNA heads

Description : Okasaki fragments are small bits of (A) RNA (B) DNA (C) DNA with RNA heads (D) RNA with DNA heads

Last Answer : Answer : C

DNA fragments upto 45 kilobases in size can be cloned in (A) Bacterial plasmids (B) Lambda phage (C) Cosmids (D) Yeast artificial chromosomes

Description : DNA fragments upto 45 kilobases in size can be cloned in (A) Bacterial plasmids (B) Lambda phage (C) Cosmids (D) Yeast artificial chromosomes

Last Answer : Answer : C

Lambda phage can be used to clone DNA fragments of the size (A) Upto 3 kilobases (B) Upto 20 kilobases (C) Upto 45 kilobases (D) Upto 1,000 kilobases

Description : Lambda phage can be used to clone DNA fragments of the size (A) Upto 3 kilobases (B) Upto 20 kilobases (C) Upto 45 kilobases (D) Upto 1,000 kilobases

Last Answer : Answer : B

DNA fragments are sealed by (A) DNA polymerase II (B) DNA ligase (C) DNA gyrase (D) DNA topoisomerase II

Description : DNA fragments are sealed by (A) DNA polymerase II (B) DNA ligase (C) DNA gyrase (D) DNA topoisomerase II

Last Answer : Answer : B

Restriction endonucleases (A) Cut RNA chains at specific locations (B) Excise introns from hnRNA (C) Remove Okazaki fragments (D) Act as defensive enzymes to protect the host bacterial DNA from DNA of foreign organisms

Description : Restriction endonucleases (A) Cut RNA chains at specific locations (B) Excise introns from hnRNA (C) Remove Okazaki fragments (D) Act as defensive enzymes to protect the host bacterial DNA from DNA of foreign organisms

Last Answer : Answer : D

On what basis the short fragments of DNA arrange themselves?

Description : On what basis the short fragments of DNA arrange themselves?

Last Answer : The short fragments of DNA get separated on the basis of their size and net electrical charge.

The restriction enzymes are used in genetic engineering, because (a) they can cut DNA at specific base sequence (b) they are nucleases that cut DNA at variable sites (c) they can degrade harmful proteins (d) they can join different DNA fragments.

Description : The restriction enzymes are used in genetic engineering, because (a) they can cut DNA at specific base sequence (b) they are nucleases that cut DNA at variable sites (c) they can degrade harmful proteins (d) they can join different DNA fragments.

Last Answer : (a) they can cut DNA at specific base sequence

DNA fragments generated by the restriction endo- nucleases in a chemical reaction can be separated by (a) electrophoresis (b) restriction mapping (c) centrifugation (d) polymerase chain reaction.

Description : DNA fragments generated by the restriction endo- nucleases in a chemical reaction can be separated by (a) electrophoresis (b) restriction mapping (c) centrifugation (d) polymerase chain reaction.

Last Answer : (a) electrophoresis

What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis ? (a) The smaller the fragment size, the farther it moves. (b) Positively charged fragments move to farther end. (c) Negatively charged fragments do not move. (d) The larger the fragment size, the farther it moves.

Description : What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis ? (a) The smaller the fragment size, the farther it moves. (b) Positively charged fragments move to farther end. ... charged fragments do not move. (d) The larger the fragment size, the farther it moves.

Last Answer : (a) The smaller the fragment size, the farther it moves

DNA fragments are (a) negatively charged (b) neutral (c) either positively or negatively charged depending on their size (d) positively charged

Description : DNA fragments are (a) negatively charged (b) neutral (c) either positively or negatively charged depending on their size (d) positively charged

Last Answer : (a) negatively charged

The DNA fragments separated on an agarose gel can be visualised after staining with (a) acetocarmine (b) aniline blue (c) ethidium bromide (d) bromophenol blue.

Description : The DNA fragments separated on an agarose gel can be visualised after staining with (a) acetocarmine (b) aniline blue (c) ethidium bromide (d) bromophenol blue.

Last Answer : (c) ethidium bromide

In gel electrophoresis, separated DNA fragments can be visualized with the help of (a) acetocarmine in bright blue light (b) ethidium bromide in UV radiation (c) acetocarmine in UV radiation (d) ethidium bromide in infrared radiation.

Description : In gel electrophoresis, separated DNA fragments can be visualized with the help of (a) acetocarmine in bright blue light (b) ethidium bromide in UV radiation (c) acetocarmine in UV radiation (d) ethidium bromide in infrared radiation.

Last Answer : (b) ethidium bromide in UV radiation

Choose the correct pair from the following. (a) Ligases - Join the two DNA molecules (b) Polymerases - Break the DNA into fragments (c) Nucleases - Separate the two strands of DNA (d) Exonucleases - Make cuts at specific positions within DNA

Description : Choose the correct pair from the following. (a) Ligases - Join the two DNA molecules (b) Polymerases - Break the DNA into fragments (c) Nucleases - Separate the two strands of DNA (d) Exonucleases - Make cuts at specific positions within DNA

Last Answer : (a) Ligases - Join the two DNA molecules

During DNA replication, Okazaki fragments are used to elongate (a) the lagging strand towards replication fork (b) the leading strand away from replication fork (c) the lagging strand away from the replication fork (d) the leading strand towards replication fork.

Description : During DNA replication, Okazaki fragments are used to elongate (a) the lagging strand towards replication fork (b) the leading strand away from replication fork (c) the lagging strand away from the replication fork (d) the leading strand towards replication fork.

Last Answer : (b) the leading strand away from replication fork

Competence refers to a bacterial cell's ability to a. Take Up DNA Fragments From The Environment. b. Be Infected By A Transducing Phage. c. Produce A Protein Product From An Engineered Gene. d. Undergo Conjugation With An F+ Cell.

Description : Competence refers to a bacterial cell's ability to a. Take Up DNA Fragments From The Environment. b. Be Infected By A Transducing Phage. c. Produce A Protein Product From An Engineered Gene. d. Undergo Conjugation With An F+ Cell.

Last Answer : a. Take Up DNA Fragments From The Environment.

Transformation refers to a. Using A Virus To Transfer DNA Fragments. b. DNA Fragments Transferred Between Live Donor And Recipient Cells. c. The Formation of an F- Recombinant Cell. d. The Transfer of Naked Fragments of DNA

Description : Transformation refers to a. Using A Virus To Transfer DNA Fragments. b. DNA Fragments Transferred Between Live Donor And Recipient Cells. c. The Formation of an F- Recombinant Cell. d. The Transfer of Naked Fragments of DNA

Last Answer : b. DNA Fragments Transferred Between Live Donor And Recipient Cells.

In the semiconservative method of replication a. Both parent strands are degraded. b. One parent strand is conserved in each of the the new dna. c. Both parent strands reform with one another. d. Okazaki fragments form both of the new molecules of DNA.

Description : In the semiconservative method of replication a. Both parent strands are degraded. b. One parent strand is conserved in each of the the new dna. c. Both parent strands reform with one another. d. Okazaki fragments form both of the new molecules of DNA.

Last Answer : b. One parent strand is conserved in each of the the new dna.

At a chromosome replication fork, the lagging strand consists of__ That is joined by___. a. RNA sequences; DNA ligase b. Okazaki Fragments; RNA Polymerase c. RNA sequences; ribosomes d. Okazaki fragments; DNA ligase

Description : At a chromosome replication fork, the lagging strand consists of__ That is joined by___. a. RNA sequences; DNA ligase b. Okazaki Fragments; RNA Polymerase c. RNA sequences; ribosomes d. Okazaki fragments; DNA ligase

Last Answer : d. Okazaki fragments; DNA ligase

In DNA sequencing, the primer A.specifies where the sequence ends B.specifies where the sequence begins C.both (a) and (b) D.generates variety of different sized fragments

Description : In DNA sequencing, the primer A.specifies where the sequence ends B.specifies where the sequence begins C.both (a) and (b) D.generates variety of different sized fragments

Last Answer : B.specifies where the sequence begins

The DNA fragments produced by the use of restriction endonucleases can be separated by

Description : 4. The DNA fragments produced by the use of restriction endonucleases can be separated by (a) polymerase chain reaction (b) gel electrophoresis (c) density gradient centrifugation (d) any of the above.

Last Answer : gel electrophoresis

During DNA replication, Okazaki fragments are used to elongate: (1) The lagging strand towards replication fork. (2) The leading strand away from replication fork. (3) The lagging strand away from the replication fork. (4) The leading strand towards replication fork.

Description : During DNA replication, Okazaki fragments are used to elongate: (1) The lagging strand towards replication fork. (2) The leading strand away from replication fork. (3) The lagging strand away from the replication fork. (4) The leading strand towards replication fork.

Last Answer : (3) The lagging strand away from the replication fork.

DNA fragments are: (1) Negatively charged (2) Neutral (3) Either positively or negatively charged depending on their size (4) Positively charged

Description : DNA fragments are: (1) Negatively charged (2) Neutral (3) Either positively or negatively charged depending on their size (4) Positively charged

Last Answer : (1) Negatively charged

The DNA fragments separated on an agarose gel can be visualised after staining with : (1) Acetocarmine (2) Aniline blue (3) Ethidium bromide (4) Bromophenol blue

Description : The DNA fragments separated on an agarose gel can be visualised after staining with : (1) Acetocarmine (2) Aniline blue (3) Ethidium bromide (4) Bromophenol blue

Last Answer : (3) Ethidium bromide

What is the technique of separation of disrupted cell components?

Description : What is the technique of separation of disrupted cell components?

Last Answer : Answer: Fractionation.

The study on washability of coal is done by using the __________ technique. (A) Tabling (B) Elutriation (C) Heavy media separation (D) None of these

Description : The study on washability of coal is done by using the __________ technique. (A) Tabling (B) Elutriation (C) Heavy media separation (D) None of these

Last Answer : (C) Heavy media separation

Name a suitable technique of separation of the components from a mixture of calcium sulphate and camphor.

Description : Name a suitable technique of separation of the components from a mixture of calcium sulphate and camphor.

Last Answer : The components from a mixture of calcium sulphate and camphor can be separated by the process of sublimation.

__________ is the separation technique used for desalination of sea water.(A) Thermal diffusion (B) Reverse osmosis (C) Adsorption (D) Absorption

Description : __________ is the separation technique used for desalination of sea water. (A) Thermal diffusion (B) Reverse osmosis (C) Adsorption (D) Absorption

Last Answer : (B) Reverse osmosis

When the mixture to be distilled has a very high boiling point and the product material is heat sensitive, the separation technique to be used is __________ distillation. (A) Continuous (B) Steam (C) Azeotropic (D) None of these

Description : When the mixture to be distilled has a very high boiling point and the product material is heat sensitive, the separation technique to be used is __________ distillation. (A) Continuous (B) Steam (C) Azeotropic (D) None of these

Last Answer : (B) Steam

Meiosis II performs (a) separation of sex chromosomes (b) synthesis of DNA and centromere (c) separation of homologous chromosomes (d) separation of chromatids.

Description : Meiosis II performs (a) separation of sex chromosomes (b) synthesis of DNA and centromere (c) separation of homologous chromosomes (d) separation of chromatids.

Last Answer : d) separation of chromatids.

How human insulin is prepared by recombined DNA technique? -Biology

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What are the restriction fragments? -Biology

Description : What are the restriction fragments? -Biology

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By using which technique, is DNA fingerprint done? -General Knowledge

Description : By using which technique, is DNA fingerprint done? -General Knowledge

Last Answer : The answer is 'Southern Blotting'

By using which technique, is DNA fingerprint done? -General Knowledge

Description : By using which technique, is DNA fingerprint done? -General Knowledge

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An analysis of chromosomal DNA using the Southern hybridization technique does not use (a) electrophoresis (b) blotting (c) autoradiography (d) PCR.

Description : An analysis of chromosomal DNA using the Southern hybridization technique does not use (a) electrophoresis (b) blotting (c) autoradiography (d) PCR.

Last Answer : (d) PCR.

Radiotracer technique shows that DNA is in (a) multi-helix stage (b) single-helix stage (c) double-helix stage (d) none of these.

Description : Radiotracer technique shows that DNA is in (a) multi-helix stage (b) single-helix stage (c) double-helix stage (d) none of these.

Last Answer : (c) double-helix stage

By using which technique, is DNA fingerprint done?

Description : By using which technique, is DNA fingerprint done?

Last Answer : Southern Blotting

6.9 For therapeutic use, growth hormone is obtained from: A. Recombinant DNA technique B. Human cadaver pituitaries C. Porcine pituitaries D. Chemical synthesis

Description : 6.9 For therapeutic use, growth hormone is obtained from: A. Recombinant DNA technique B. Human cadaver pituitaries C. Porcine pituitaries D. Chemical synthesis

Last Answer : A. Recombinant DNA technique

Astronomy fans anyone traveling to Coloma Ca. to search for the Lyrid meteor fragments?

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