Description : Recombinant DNA is achieved by cleaving the pro-DNAs by (a) ligase (b) restriction endonuclease (c) primase (d) exonucleases.
Last Answer : (b) restriction endonuclease
Description : A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using (a) EcoRI (b) Taq polymerase (c) polymerase III (d) ligase.
Last Answer : (d) ligase.
Description : A seals sticky ends of recombinant DNA segments. a. DNA Ligase b. restriction endonuclease c. protease d. RNA polymerase
Last Answer : b. restriction endonuclease
Description : 7. In a recombinant DNA technology a plasmid vector must be cleaved by a) the same enzyme that leaves the donor gene b) modified DNA ligase c) a heated alkaline solution d) four separate enzymes
Last Answer : a) the same enzyme that leaves the donor gene
Description : Role of restriction enzymes and DNA ligase -Biology
Last Answer : answer:
Description : How are restriction enzymes used to make recombinant DNA? -Biology
Description : Which pair of enzymes is necessary to make recombinant DNA? -Biology
Description : What is recombinant DNA? -Biology
Description : What is recombinant DNA technology? -Biology
Description : Give some examples of recombinant DNA technology applications. -Biology
Description : Name the two types of 'biological tools' used in recombinant DNA technology. -Biology
Description : What is a recombinant DNA vaccine? -Biology
Description : Give some examples of recombinant DNA vaccine. -Biology
Description : Explain the term Recombinant DNA. -Biology
Description : Why are bacteria used in recombinant DNA technology? -Biology
Description : Enzymes used in recombinant DNA technology -Biology
Description : Recombinant DNA directly produces changes in -Biology
Description : Recombinant DNA food -Biology
Description : Recombinant DNA construction -Biology
Description : Why is recombinant DNA important? -Biology
Description : Recombinant DNA disadvantages -Biology
Description : Applied use of molecular biology and recombinant DNA Technology known as
Last Answer : Ans. Biotechnology
Description : Restriction endonucleases are enzymes which (a) make cuts at specific positions within the DNA molecule (b) recognize a specific nucleotide sequence for binding of DNA ligase (c) restrict the action of the enzyme DNA polymerase (d) remove nucleotides from the ends of the DNA molecule
Last Answer : (a) make cuts at specific positions within the DNA molecule
Description : What is the role of the DNA ligase in DNA replication?
Last Answer : It joins Okazaki fragments
Description : $ The enzymes which joins short pieces DNA in the laggins strand is called ligase. ! Lagging strand and leading strand are formed due to replication f
Last Answer : $ The enzymes which joins short pieces DNA in the laggins strand is called ligase. ! Lagging strand and ... wrong D. If both As and R are wrong.
Description : What is the role of the DNA ligase in DNA replication apex?
Last Answer : It ties the chunks of DNA together.
Description : In the biosynthesis of c-DNA, the joining enzyme ligase requires (A) GTP (B) ATP (C) CTP (D) UTP
Last Answer : Answer : B
Description : DNA ligase of E. coli requires which of the following co-factors? (A) FAD (B) NAD+ (C) NADP+ (D) NADH
Description : Xeroderma pigmentosum results from a defect in (A) uvr ABC excinuclease (B) DNA polymerase I (C) DNA ligase (D) All of these
Last Answer : Answer : A
Description : Damage to DNA caused by ultraviolet radiation can be repaired by (A) uvr ABC excinuclease (B) DNA polymerase I (C) DNA ligase (D) All of these
Last Answer : Answer : D
Description : Negative supercoils are introduced in DNA by (A) Helicase (B) DNA ligase (C) DNA gyrase (D) DNA polymerase III holoenzyme
Last Answer : Answer : C
Description : DNA fragments are sealed by (A) DNA polymerase II (B) DNA ligase (C) DNA gyrase (D) DNA topoisomerase II
Description : The enzyme DNA ligase (A) Introduces superhelical twists (B) Connects the end of two DNA chains (C) Unwinds the double helix (D) Synthesises RNA primers
Description : The α-amino group of the new amino acyl tRNA in the A site carries out a nucleophilic attack on the esterified carboxyl group of the peptidyl tRNA occupying the P site. This reaction is catalysed by (A) DNA polymerase (B) RNA polymerase (C) Peptidyl transferase (D) DNA ligase
Description : In DNA replication the enzyme required in the first step is (A) DNA directed polymerase (B) Unwinding proteins (C) DNA polymerase (D) DNA ligase
Description : An eazyme produced by HIV that allows the integration of HIV DNA into the host cell's DNA is (1) Ligase (2) Integrase (3) Helicase (4) DNA gyrase
Last Answer : (2) Integrase Explanation: HIV's enzyme for inserting the DNA version of its genome into the host cell DNA is called its "integrase". HIV-1 integrase catalyzes the "cut-and-paste" action of ... to the clipped ends. It is also produced by viruses containing double-stranded DNAs for the same purpose.
Description : Which of the following cut the DNA from specific places? (a) E.coli restriction endonuclease I (b) Ligase (c) Exonuclease (d) Alkaline phosphate
Last Answer : (a) E.coli restriction endonuclease I
Description : Which of the following enzymes are used to join bits of DNA? (a) Ligase (b) Primase (c) DNA polymerase (d) Endonuclease
Last Answer : a) Ligase
Description : The linking of antibiotic resistance gene with the plasmid vector became possible with (a) DNA polymerase (b) exonucleases (c) DNA ligase (d) endonucleases.
Last Answer : (c) DNA ligase
Description : Manipulation of DNA in genetic engineering became possible due to the discovery of (a) restriction endonuclease (b) DNA ligase (c) transcriptase (d) primase
Last Answer : (a) restriction endonuclease
Description : Name the enzyme that facilitates opening of DNA helix during transcription. (a) DNA ligase (b) DNA helicase (c) DNA polymerase (d) RNA polymerase
Last Answer : b) DNA helicase
Description : There are special proteins that help to open up DNA double helix in front of the replication fork. These proteins are (a) DNA ligase (b) DNA topoisomerase I (c) DNA gyrase (d) DNA polymerase I.
Last Answer : (c) DNA gyrase
Description : Which of the following are examples of repair mechanisms in cells? a. mismatch repair b. excision repair c. DNA polymerase d. DNA ligase e. all are important in fixing mutations
Last Answer : e. all are important in fixing mutations
Description : At a chromosome replication fork, the lagging strand consists of__ That is joined by___. a. RNA sequences; DNA ligase b. Okazaki Fragments; RNA Polymerase c. RNA sequences; ribosomes d. Okazaki fragments; DNA ligase
Last Answer : d. Okazaki fragments; DNA ligase
Description : The enzyme adds complementary bases to the DNA template strand during replication. a. Ligase b. Helicase c. DNA polymerase III d. RNA polymerase
Last Answer : d. RNA polymerase
Description : Which of the following molecule catalyzes the transpeptidation reaction? A.RNA polymerase B.Peptidyl transferase C.DNA ligase D.DNA polymerase
Last Answer : B.Peptidyl transferase
Description : Which of the following molecule catalyzes the transpeptidation reaction? A- RNA Polymerase B- Peptidlytransferase C- DNA ligase D- DNA polymerase
Last Answer : Peptidlytransferase
Description : Which of the following molecule catalyzes the transpeptidation reaction? A- RNA polymerase B- .Peptidyltransferase C- DNA ligase D- .DNA polymerase
Last Answer : .Peptidyltransferase