Description : Recombinant DNA is achieved by cleaving the pro-DNAs by (a) ligase (b) restriction endonuclease (c) primase (d) exonucleases.
Last Answer : (b) restriction endonuclease
Description : Manipulation of DNA in genetic engineering became possible due to the discovery of (a) restriction endonuclease (b) DNA ligase (c) transcriptase (d) primase
Last Answer : (a) restriction endonuclease
Description : A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using (a) EcoRI (b) Taq polymerase (c) polymerase III (d) ligase.
Last Answer : (d) ligase.
Description : A seals sticky ends of recombinant DNA segments. a. DNA Ligase b. restriction endonuclease c. protease d. RNA polymerase
Last Answer : b. restriction endonuclease
Description : Which of the following reunites the exon segments after RNA splicing? (a) RNA polymerase (b) RNA primase (c) RNA ligase (d) RNA proteose
Last Answer : a) RNA polymerase
Description : Which of the following reunites the exon segments after RNA splicing? (a) RNA polymerase (b) RNA primase (c) RNA ligase (d) RNA proteoses
Last Answer : (c) RNA ligase
Description : Restriction endonucleases are enzymes which (a) make cuts at specific positions within the DNA molecule (b) recognize a specific nucleotide sequence for binding of DNA ligase (c) restrict the action of the enzyme DNA polymerase (d) remove nucleotides from the ends of the DNA molecule
Last Answer : (a) make cuts at specific positions within the DNA molecule
Description : Enzyme which can break and seal the DNA strand a) Topoisomease II (b) Helicase (c) Primase (d) Restriction endonuclease
Last Answer : a) Topoisomease II
Description : In the process of transcription in bacterial cells (A) Initiation requires rho protein (B) RNA polymerase incorporates methylated bases in correct sequence (C) Both the sigma unit and core ... RNA polymerase are required for accurate promotor site binding (D) Primase is necessary for initiation
Last Answer : Answer : C
Description : Which of the following cut the DNA from specific places? (a) E.coli restriction endonuclease I (b) Ligase (c) Exonuclease (d) Alkaline phosphate
Last Answer : (a) E.coli restriction endonuclease I
Description : 1. Bacteria protect themselves from viruses by fragmenting viral DNA with a. Ligase b. Endonuclease c. Exonuclease d. Gyrase
Last Answer : Ans: Endonuclease.
Description : In addition to Taq polymerase, polymerase chain reaction requires all of the following except (A) A template DNA (B) Deoxyribonucleoside triphosphates (C) Primers (D) Primase
Last Answer : Answer : D
Description : Primase activity is present in (A) DNA polymerase II (B) DNA polymerase α (C) DNA polymerase β (D) DNA polymerase δ
Last Answer : Answer : B
Description : RNA primer is formed by the enzyme: (A) Ribonuclease (B) Primase (C) DNA polymerase I (D) DNA polymerase III
Description : The enzyme which catalyzes the unwinding of DNA helix during replication is: (1) Primase (2) Topoisomerase (3) Helicase (4) DNA polymerase
Last Answer : (3) Helicase Explanation: A type of helicase enzyme called DNA helicase catalyzes the unwinding of the DNA helix at the time of replication. It uses the energy released through hydrolysis of ATP ( ... The enzyme is needed to separate the two strands of DNA also during transcription and DNA repair.
Description : One of the first enzymes synthesized by many bacteriophage is __________ , an RNA dependent RNA polymerase A.RNA transcriptase B.RNA polymerase C.RNA ligase D.RNA replicase
Last Answer : D.RNA replicase
Description : Xeroderma pigmentosum results from a defect in (A) uvr ABC excinuclease (B) DNA polymerase I (C) DNA ligase (D) All of these
Last Answer : Answer : A
Description : Damage to DNA caused by ultraviolet radiation can be repaired by (A) uvr ABC excinuclease (B) DNA polymerase I (C) DNA ligase (D) All of these
Description : Negative supercoils are introduced in DNA by (A) Helicase (B) DNA ligase (C) DNA gyrase (D) DNA polymerase III holoenzyme
Description : DNA fragments are sealed by (A) DNA polymerase II (B) DNA ligase (C) DNA gyrase (D) DNA topoisomerase II
Description : The α-amino group of the new amino acyl tRNA in the A site carries out a nucleophilic attack on the esterified carboxyl group of the peptidyl tRNA occupying the P site. This reaction is catalysed by (A) DNA polymerase (B) RNA polymerase (C) Peptidyl transferase (D) DNA ligase
Description : In DNA replication the enzyme required in the first step is (A) DNA directed polymerase (B) Unwinding proteins (C) DNA polymerase (D) DNA ligase
Description : The linking of antibiotic resistance gene with the plasmid vector became possible with (a) DNA polymerase (b) exonucleases (c) DNA ligase (d) endonucleases.
Last Answer : (c) DNA ligase
Description : Name the enzyme that facilitates opening of DNA helix during transcription. (a) DNA ligase (b) DNA helicase (c) DNA polymerase (d) RNA polymerase
Last Answer : b) DNA helicase
Description : There are special proteins that help to open up DNA double helix in front of the replication fork. These proteins are (a) DNA ligase (b) DNA topoisomerase I (c) DNA gyrase (d) DNA polymerase I.
Last Answer : (c) DNA gyrase
Description : Which of the following are examples of repair mechanisms in cells? a. mismatch repair b. excision repair c. DNA polymerase d. DNA ligase e. all are important in fixing mutations
Last Answer : e. all are important in fixing mutations
Description : At a chromosome replication fork, the lagging strand consists of__ That is joined by___. a. RNA sequences; DNA ligase b. Okazaki Fragments; RNA Polymerase c. RNA sequences; ribosomes d. Okazaki fragments; DNA ligase
Last Answer : d. Okazaki fragments; DNA ligase
Description : The enzyme adds complementary bases to the DNA template strand during replication. a. Ligase b. Helicase c. DNA polymerase III d. RNA polymerase
Last Answer : d. RNA polymerase
Description : Which of the following molecule catalyzes the transpeptidation reaction? A.RNA polymerase B.Peptidyl transferase C.DNA ligase D.DNA polymerase
Last Answer : B.Peptidyl transferase
Description : Which of the following molecule catalyzes the transpeptidation reaction? A- RNA Polymerase B- Peptidlytransferase C- DNA ligase D- DNA polymerase
Last Answer : Peptidlytransferase
Description : Which of the following molecule catalyzes the transpeptidation reaction? A- RNA polymerase B- .Peptidyltransferase C- DNA ligase D- .DNA polymerase
Last Answer : .Peptidyltransferase
Description : The removal of which enzyme affects the synthesis of hnRNA in eukaryotes a) RNA polymerase II b) RNA primase c) RNA polymerase III d) RNA polymerase I
Last Answer : a) RNA polymerase II
Description : Role of restriction enzymes and DNA ligase -Biology
Last Answer : answer:
Description : $ The enzymes which joins short pieces DNA in the laggins strand is called ligase. ! Lagging strand and leading strand are formed due to replication f
Last Answer : $ The enzymes which joins short pieces DNA in the laggins strand is called ligase. ! Lagging strand and ... wrong D. If both As and R are wrong.
Description : 7. In a recombinant DNA technology a plasmid vector must be cleaved by a) the same enzyme that leaves the donor gene b) modified DNA ligase c) a heated alkaline solution d) four separate enzymes
Last Answer : a) the same enzyme that leaves the donor gene
Description : The enzyme used in polymerase chain reaction (PCR) is (A) Taq polymerase (B) RNA polymerase (C) Ribonuclease (D) Endonuclease
Description : A scientist wants to make a dna fingerprint and she used polymerase chain reaction and restriction enzymes what should her next step be?
Last Answer : use restriction enzymes is the right answer for apex :)
Description : DNA contains some palindromic sequences which (A) Mark the site for the formation of replication forks (B) Direct DNA polymerase to turn back to replicate the other strand (C) Are recognized by restriction enzymes (D) Are found only in bacterial DNA
Description : Which of the following is not required for any of the techniques of DNA fingerprinting available at present? (a) Restriction enzymes (b) DNA-DNA hybridisation (c) Polymerase chain reaction (d) Zinc finger analysis
Last Answer : (d) Zinc finger analysis
Description : The following are true about DNA synthesis: a. it requires DNA polymerase b. reverse transcriptase enzymes are involved c. moves in a 5'---> 3' direction d. the rate of error in DNA synthesis is 1 in 105 base pairs
Last Answer : it requires DNA polymerase
Description : What is the role of the enzyme DNA primase?
Last Answer : Primase is an enzyme that synthesizes short RNA sequences called primers which serve as a starting point for DNA synthesis. Since primase produces RNA molecules, the enzyme is a type of RNA ... strand of nucleotides. Therefore, primase serves to prime and lay a foundation for DNA synthesis.
Description : The enzyme which catalyzes the unwinding of DNA helix during replication is: (1) Primase (2) Topoisomerase (3) Helicase (4) DNApolymerase
Last Answer : C option is the answer
Description : In sticky ends produced by restriction endonucleases (A) The 2 strands of DNA are joined to each other (B) The DNA strands stick to the restriction endonuclease (C) The ends of a double stranded fragment are overlapping (D) The ends of a double stranded fragment are non overlapping
Description : Genetic engineering requires enzyme: (A) DNA ase (B) Amylase (C) Lipase (D) Restriction endonuclease
Description : Restriction endonuclease (a) synthesizes DNA (b) cuts the DNA molecule randomly (c) cuts the DNA molecule at specific sites (d) restricts the synthesis of DNA inside the nucleus.
Last Answer : (c) cuts the DNA molecule at specific sites
Description : Following statements describe the characteristics of the enzyme restriction endonuclease. Identify the incorrect statement. (a) The enzyme recognises a specific palindromic nucleotide sequence in the DNA. (b) ... (d) The enzyme cuts the sugar-phosphate backbone at specific sites on each strand.
Last Answer : (c) The enzyme binds DNA at specific sites and cuts only one of the two strands.
Description : The restriction enzymes are used in genetic engineering, because (a) they can cut DNA at specific base sequence (b) they are nucleases that cut DNA at variable sites (c) they can degrade harmful proteins (d) they can join different DNA fragments.
Last Answer : (a) they can cut DNA at specific base sequence