Description : A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using (a) EcoRI (b) Taq polymerase (c) polymerase III (d) ligase.
Last Answer : (d) ligase.
Description : Recombinant DNA is achieved by cleaving the pro-DNAs by (a) ligase (b) restriction endonuclease (c) primase (d) exonucleases.
Last Answer : (b) restriction endonuclease
Description : In sticky ends produced by restriction endonucleases (A) The 2 strands of DNA are joined to each other (B) The DNA strands stick to the restriction endonuclease (C) The ends of a double stranded fragment are overlapping (D) The ends of a double stranded fragment are non overlapping
Last Answer : Answer : C
Description : Restriction endonucleases are enzymes which (a) make cuts at specific positions within the DNA molecule (b) recognize a specific nucleotide sequence for binding of DNA ligase (c) restrict the action of the enzyme DNA polymerase (d) remove nucleotides from the ends of the DNA molecule
Last Answer : (a) make cuts at specific positions within the DNA molecule
Description : At a chromosome replication fork, the lagging strand consists of__ That is joined by___. a. RNA sequences; DNA ligase b. Okazaki Fragments; RNA Polymerase c. RNA sequences; ribosomes d. Okazaki fragments; DNA ligase
Last Answer : d. Okazaki fragments; DNA ligase
Description : The enzyme adds complementary bases to the DNA template strand during replication. a. Ligase b. Helicase c. DNA polymerase III d. RNA polymerase
Last Answer : d. RNA polymerase
Description : Which of the following molecule catalyzes the transpeptidation reaction? A.RNA polymerase B.Peptidyl transferase C.DNA ligase D.DNA polymerase
Last Answer : B.Peptidyl transferase
Description : Which of the following molecule catalyzes the transpeptidation reaction? A- RNA Polymerase B- Peptidlytransferase C- DNA ligase D- DNA polymerase
Last Answer : Peptidlytransferase
Description : Which of the following molecule catalyzes the transpeptidation reaction? A- RNA polymerase B- .Peptidyltransferase C- DNA ligase D- .DNA polymerase
Last Answer : .Peptidyltransferase
Description : Which of the following cut the DNA from specific places? (a) E.coli restriction endonuclease I (b) Ligase (c) Exonuclease (d) Alkaline phosphate
Last Answer : (a) E.coli restriction endonuclease I
Description : Manipulation of DNA in genetic engineering became possible due to the discovery of (a) restriction endonuclease (b) DNA ligase (c) transcriptase (d) primase
Last Answer : (a) restriction endonuclease
Description : Which of the following enzymes are used to join bits of DNA? (a) Ligase (b) Primase (c) DNA polymerase (d) Endonuclease
Last Answer : a) Ligase
Description : Which of the following reunites the exon segments after RNA splicing? (a) RNA polymerase (b) RNA primase (c) RNA ligase (d) RNA proteose
Last Answer : a) RNA polymerase
Description : Which of the following reunites the exon segments after RNA splicing? (a) RNA polymerase (b) RNA primase (c) RNA ligase (d) RNA proteoses
Last Answer : (c) RNA ligase
Description : One of the first enzymes synthesized by many bacteriophage is __________ , an RNA dependent RNA polymerase A.RNA transcriptase B.RNA polymerase C.RNA ligase D.RNA replicase
Last Answer : D.RNA replicase
Description : Which of the following is a restriction endonuclease? (a) DNase I (b) RNase (c) Hind II (d) Protease
Last Answer : (c) Hind II
Description : Which of the following are examples of repair mechanisms in cells? a. mismatch repair b. excision repair c. DNA polymerase d. DNA ligase e. all are important in fixing mutations
Last Answer : e. all are important in fixing mutations
Description : Without restriction endonucleases, it would be very difficult to a. Force Plasmids into Bacteria. b. Chemically Open Dna Molecules. c. Replicate Dna In A Recombinant Cell. d. Bring About Mutations In Bacteria.
Last Answer : b. Chemically Open Dna Molecules.
Description : The α-amino group of the new amino acyl tRNA in the A site carries out a nucleophilic attack on the esterified carboxyl group of the peptidyl tRNA occupying the P site. This reaction is catalysed by (A) DNA polymerase (B) RNA polymerase (C) Peptidyl transferase (D) DNA ligase
Description : Name the enzyme that facilitates opening of DNA helix during transcription. (a) DNA ligase (b) DNA helicase (c) DNA polymerase (d) RNA polymerase
Last Answer : b) DNA helicase
Description : Which complementary sequence would NOT be recognized by a restriction endonucLease? a. GAATTC CTTAAG b. AAGCTT TTCGAA c. GTCGAC CAGCTG d. AATTCCTTAAGG
Last Answer : d. AATTCCTTAAGG
Description : Which of the following statement is correct? A- The size and sequence of introns can be deduced from the cDNA sequence B- Restriction endonuclease can cleave ss and dsDNA both C- Restriction ... sequenceof a gene D- Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide
Last Answer : Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide
Description : Which of the following statement is correct? A- The size and sequence of introns can be deduced from the cDNA sequence B- .Restriction endonuclease can cleave ss and dsDNA both C- Restriction ... of a Gene D- Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide
Description : Assetion: Restriction enzymes cut the strand 27 of DNA to produce sticky ends Reason : Stickiness of the ends faciliates the action of the enzyme DNA
Last Answer : Assetion: Restriction enzymes cut the strand 27 of DNA to produce sticky ends Reason : Stickiness of ... D. If both assertion and reason are false
Description : All of the following statements about restriction endonucleases are true except: (A) They are present in bacteria (B) They act on double stranded DNA (C) They recognize palindromic sequences (D) They always produce sticky ends
Last Answer : Answer : D
Description : Identify the wrong statement with regard to restriction enzymes. (a) Each restriction enzyme functions by inspecting the length of a DNA sequence. (b) They cut the strand of DNA at palindromic sites. (c) They are useful in genetic engineering. (d) Sticky ends can be joined by using DNA ligases.
Last Answer : d) Sticky ends can be joined by using DNA ligases.
Description : 1. Bacteria protect themselves from viruses by fragmenting viral DNA with a. Ligase b. Endonuclease c. Exonuclease d. Gyrase
Last Answer : Ans: Endonuclease.
Description : Negative regulation of protein synthesis is accomplished by A- allosteric inhibition B- the binding of RNA polymerase to the promoter C- the binding of a repressor to the DNA D- the binding of a repressor to the RNA polymerase
Last Answer : the binding of a repressor to the DNA
Description : The role of molecular chaperones is to A-facilitate binding of ribosomes to mRNA B-degrade newly synthesized polypeptides that contain inaccurate sequences C-.facilitate binding of RNA polymerase to DNA D-aid a newly synthesized polypeptide in folding to its proper shape
Last Answer : -aid a newly synthesized polypeptide in folding to its proper shape
Last Answer : aid a newly synthesized polypeptide in folding to its proper shape
Description : The enzyme used in polymerase chain reaction (PCR) is (A) Taq polymerase (B) RNA polymerase (C) Ribonuclease (D) Endonuclease
Description : The correct statement concerning RNA and DNA polymerases is (A) RNA polymerase use nucleoside diphosphates (B) RNA polymerase require primers and add bases at 5' end of the growing polynucleotide ... and DNA polymerases can add nucleotides only at the 3' end of the growing polynucleotide chain
Description : Why is ligase needed to make recombinant DNA? -Biology
Last Answer : answer:
Description : 7. In a recombinant DNA technology a plasmid vector must be cleaved by a) the same enzyme that leaves the donor gene b) modified DNA ligase c) a heated alkaline solution d) four separate enzymes
Last Answer : a) the same enzyme that leaves the donor gene
Description : Viropexis takes place in the phagocytic vacuoles and is due to the action of Enzymes A- DNA gyrase B- lysosomal protease C- lysosomal lipase D- all of these
Last Answer : lysosomal protease
Description : Genetic engineering requires enzyme: (A) DNA ase (B) Amylase (C) Lipase (D) Restriction endonuclease
Description : Restriction endonuclease (a) synthesizes DNA (b) cuts the DNA molecule randomly (c) cuts the DNA molecule at specific sites (d) restricts the synthesis of DNA inside the nucleus.
Last Answer : (c) cuts the DNA molecule at specific sites
Description : Following statements describe the characteristics of the enzyme restriction endonuclease. Identify the incorrect statement. (a) The enzyme recognises a specific palindromic nucleotide sequence in the DNA. (b) ... (d) The enzyme cuts the sugar-phosphate backbone at specific sites on each strand.
Last Answer : (c) The enzyme binds DNA at specific sites and cuts only one of the two strands.
Description : Enzyme which can break and seal the DNA strand a) Topoisomease II (b) Helicase (c) Primase (d) Restriction endonuclease
Last Answer : a) Topoisomease II
Description : Which one of the following hydrolyses internal phosphodiester bonds in a polynucleotide chain? (a) Lipase (b) Protease (c) Endonuclease (d) Exonuclease
Last Answer : (c) Endonuclease
Last Answer : c) G + C / A + T
Description : What is the term for manipulating the genes of organisms to introduce new characteristics? a. Genomics b. Genetic engineering c. Recombinant RNA d. Genetic resistance
Last Answer : b. Genetic engineering
Description : Role of restriction enzymes and DNA ligase -Biology
Description : Which one of the following binds to specific nucleotide sequences that are upstream and most distant from the start site? (A) RNA polymerase (B) Repressor (C) Inducer (D) Restriction
Last Answer : Answer : A
Description : Which one of the following binds to specific nucleotide sequences? (A) RNA polymerase (B) Repressor (C) Inducer (D) Restriction
Description : Xeroderma pigmentosum results from a defect in (A) uvr ABC excinuclease (B) DNA polymerase I (C) DNA ligase (D) All of these
Description : Damage to DNA caused by ultraviolet radiation can be repaired by (A) uvr ABC excinuclease (B) DNA polymerase I (C) DNA ligase (D) All of these