Description : Restriction endonucleases are enzymes which (a) make cuts at specific positions within the DNA molecule (b) recognize a specific nucleotide sequence for binding of DNA ligase (c) restrict the action of the enzyme DNA polymerase (d) remove nucleotides from the ends of the DNA molecule
Last Answer : (a) make cuts at specific positions within the DNA molecule
Description : Recombinant DNA is achieved by cleaving the pro-DNAs by (a) ligase (b) restriction endonuclease (c) primase (d) exonucleases.
Last Answer : (b) restriction endonuclease
Description : Which of the following cut the DNA from specific places? (a) E.coli restriction endonuclease I (b) Ligase (c) Exonuclease (d) Alkaline phosphate
Last Answer : (a) E.coli restriction endonuclease I
Description : A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using (a) EcoRI (b) Taq polymerase (c) polymerase III (d) ligase.
Last Answer : (d) ligase.
Description : Manipulation of DNA in genetic engineering became possible due to the discovery of (a) restriction endonuclease (b) DNA ligase (c) transcriptase (d) primase
Last Answer : (a) restriction endonuclease
Description : A seals sticky ends of recombinant DNA segments. a. DNA Ligase b. restriction endonuclease c. protease d. RNA polymerase
Last Answer : b. restriction endonuclease
Description : $ The enzymes which joins short pieces DNA in the laggins strand is called ligase. ! Lagging strand and leading strand are formed due to replication f
Last Answer : $ The enzymes which joins short pieces DNA in the laggins strand is called ligase. ! Lagging strand and ... wrong D. If both As and R are wrong.
Description : Which of the following enzymes are used to join bits of DNA? (a) Ligase (b) Primase (c) DNA polymerase (d) Endonuclease
Last Answer : a) Ligase
Description : 7. In a recombinant DNA technology a plasmid vector must be cleaved by a) the same enzyme that leaves the donor gene b) modified DNA ligase c) a heated alkaline solution d) four separate enzymes
Last Answer : a) the same enzyme that leaves the donor gene
Description : How are restriction enzymes used to make recombinant DNA? -Biology
Last Answer : answer:
Description : Restriction enzymes and DNA methylation -Biology
Description : Why are restriction enzymes used for DNA fingerprinting? -Biology
Description : What is the role of the DNA ligase in DNA replication?
Last Answer : It joins Okazaki fragments
Description : What is the role of the DNA ligase in DNA replication apex?
Last Answer : It ties the chunks of DNA together.
Description : What role do restriction enzymes play in analysis of Edna?
Last Answer : Feel Free to Answer
Description : Assetion: Restriction enzymes cut the strand 27 of DNA to produce sticky ends Reason : Stickiness of the ends faciliates the action of the enzyme DNA
Last Answer : Assetion: Restriction enzymes cut the strand 27 of DNA to produce sticky ends Reason : Stickiness of ... D. If both assertion and reason are false
Description : A scientist wants to make a dna fingerprint and she used polymerase chain reaction and restriction enzymes what should her next step be?
Last Answer : use restriction enzymes is the right answer for apex :)
Description : DNA contains some palindromic sequences which (A) Mark the site for the formation of replication forks (B) Direct DNA polymerase to turn back to replicate the other strand (C) Are recognized by restriction enzymes (D) Are found only in bacterial DNA
Last Answer : Answer : C
Description : Restriction endonucleases (A) Cut RNA chains at specific locations (B) Excise introns from hnRNA (C) Remove Okazaki fragments (D) Act as defensive enzymes to protect the host bacterial DNA from DNA of foreign organisms
Last Answer : Answer : D
Description : Read the following four statements (A-D) about certain mistakes in two of them. (A) The first transgenic buffalo, Rosie produced milk which was human alpha-lactalbumin enriched. (B) Restriction enzymes are used in isolation of DNA ... mistakes? (a) B and C (b) C and D (c) A and C (d) A and B
Last Answer : (d) A and B
Description : PCR and restriction fragment length polymorphism are the methods for (a) study of enzymes (b) genetic transformation (c) DNA sequencing (d) genetic fingerprinting.
Last Answer : (d) genetic fingerprinting.
Description : During the process of isolation of DNA, chilled ethanol is added to (a) precipitate DNA (b) break open the cell to release DNA (c) facilitate action of restriction enzymes (d) remove proteins such as histones.
Last Answer : a) precipitate DNA
Description : The restriction enzymes are used in genetic engineering, because (a) they can cut DNA at specific base sequence (b) they are nucleases that cut DNA at variable sites (c) they can degrade harmful proteins (d) they can join different DNA fragments.
Last Answer : (a) they can cut DNA at specific base sequence
Description : Identify the wrong statement with regard to restriction enzymes. (a) Each restriction enzyme functions by inspecting the length of a DNA sequence. (b) They cut the strand of DNA at palindromic sites. (c) They are useful in genetic engineering. (d) Sticky ends can be joined by using DNA ligases.
Last Answer : d) Sticky ends can be joined by using DNA ligases.
Description : The cutting of DNA at specific locations became possible with the discovery of (a) selectable markers (b) ligases (c) restriction enzymes (d) probes.
Last Answer : (c) restriction enzymes
Description : Which of the following is not required for any of the techniques of DNA fingerprinting available at present? (a) Restriction enzymes (b) DNA-DNA hybridisation (c) Polymerase chain reaction (d) Zinc finger analysis
Last Answer : (d) Zinc finger analysis
Description : One of the first enzymes synthesized by many bacteriophage is __________ , an RNA dependent RNA polymerase A.RNA transcriptase B.RNA polymerase C.RNA ligase D.RNA replicase
Last Answer : D.RNA replicase
Description : Why is ligase needed to make recombinant DNA? -Biology
Description : Name some restriction enzymes. -Biology
Description : What is the significance of recognition sequences of restriction enzymes? -Biology
Description : Nomenclature of restriction enzymes. -Biology
Description : What is the enzymatic function of restriction enzymes? -Biology
Description : What is the source of restriction enzymes? -Biology
Description : What are restriction enzymes used for? -Biology
Description : Why are restriction enzymes called molecular scissors? -Biology
Description : Why is cutting enzymes known as the restriction enzymes? -Biology
Description : In the biosynthesis of c-DNA, the joining enzyme ligase requires (A) GTP (B) ATP (C) CTP (D) UTP
Last Answer : Answer : B
Description : DNA ligase of E. coli requires which of the following co-factors? (A) FAD (B) NAD+ (C) NADP+ (D) NADH
Description : Xeroderma pigmentosum results from a defect in (A) uvr ABC excinuclease (B) DNA polymerase I (C) DNA ligase (D) All of these
Last Answer : Answer : A
Description : Damage to DNA caused by ultraviolet radiation can be repaired by (A) uvr ABC excinuclease (B) DNA polymerase I (C) DNA ligase (D) All of these
Description : Negative supercoils are introduced in DNA by (A) Helicase (B) DNA ligase (C) DNA gyrase (D) DNA polymerase III holoenzyme
Description : DNA fragments are sealed by (A) DNA polymerase II (B) DNA ligase (C) DNA gyrase (D) DNA topoisomerase II
Description : The enzyme DNA ligase (A) Introduces superhelical twists (B) Connects the end of two DNA chains (C) Unwinds the double helix (D) Synthesises RNA primers
Description : The α-amino group of the new amino acyl tRNA in the A site carries out a nucleophilic attack on the esterified carboxyl group of the peptidyl tRNA occupying the P site. This reaction is catalysed by (A) DNA polymerase (B) RNA polymerase (C) Peptidyl transferase (D) DNA ligase
Description : In DNA replication the enzyme required in the first step is (A) DNA directed polymerase (B) Unwinding proteins (C) DNA polymerase (D) DNA ligase
Description : An eazyme produced by HIV that allows the integration of HIV DNA into the host cell's DNA is (1) Ligase (2) Integrase (3) Helicase (4) DNA gyrase
Last Answer : (2) Integrase Explanation: HIV's enzyme for inserting the DNA version of its genome into the host cell DNA is called its "integrase". HIV-1 integrase catalyzes the "cut-and-paste" action of ... to the clipped ends. It is also produced by viruses containing double-stranded DNAs for the same purpose.
Description : The linking of antibiotic resistance gene with the plasmid vector became possible with (a) DNA polymerase (b) exonucleases (c) DNA ligase (d) endonucleases.
Last Answer : (c) DNA ligase
Description : Name the enzyme that facilitates opening of DNA helix during transcription. (a) DNA ligase (b) DNA helicase (c) DNA polymerase (d) RNA polymerase
Last Answer : b) DNA helicase