Description : Manipulation of DNA in genetic engineering became possible due to the discovery of (a) restriction endonuclease (b) DNA ligase (c) transcriptase (d) primase
Last Answer : (a) restriction endonuclease
Description : A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using (a) EcoRI (b) Taq polymerase (c) polymerase III (d) ligase.
Last Answer : (d) ligase.
Description : A seals sticky ends of recombinant DNA segments. a. DNA Ligase b. restriction endonuclease c. protease d. RNA polymerase
Last Answer : b. restriction endonuclease
Description : Which of the following enzymes are used to join bits of DNA? (a) Ligase (b) Primase (c) DNA polymerase (d) Endonuclease
Last Answer : a) Ligase
Description : Enzyme which can break and seal the DNA strand a) Topoisomease II (b) Helicase (c) Primase (d) Restriction endonuclease
Last Answer : a) Topoisomease II
Description : Which of the following cut the DNA from specific places? (a) E.coli restriction endonuclease I (b) Ligase (c) Exonuclease (d) Alkaline phosphate
Last Answer : (a) E.coli restriction endonuclease I
Description : The linking of antibiotic resistance gene with the plasmid vector became possible with (a) DNA polymerase (b) exonucleases (c) DNA ligase (d) endonucleases.
Last Answer : (c) DNA ligase
Description : he molecular scissors use3d to cut DNA into specific genes of interest are called (a) Exonucleases (b) Restriction endonucleases (c) Ligases (d) Polymerases
Last Answer : (b) Restriction endonucleases
Description : 1. Bacteria protect themselves from viruses by fragmenting viral DNA with a. Ligase b. Endonuclease c. Exonuclease d. Gyrase
Last Answer : Ans: Endonuclease.
Description : Why is ligase needed to make recombinant DNA? -Biology
Last Answer : answer:
Description : 7. In a recombinant DNA technology a plasmid vector must be cleaved by a) the same enzyme that leaves the donor gene b) modified DNA ligase c) a heated alkaline solution d) four separate enzymes
Last Answer : a) the same enzyme that leaves the donor gene
Description : In sticky ends produced by restriction endonucleases (A) The 2 strands of DNA are joined to each other (B) The DNA strands stick to the restriction endonuclease (C) The ends of a double stranded fragment are overlapping (D) The ends of a double stranded fragment are non overlapping
Last Answer : Answer : C
Description : Genetic engineering requires enzyme: (A) DNA ase (B) Amylase (C) Lipase (D) Restriction endonuclease
Last Answer : Answer : D
Description : Restriction endonuclease (a) synthesizes DNA (b) cuts the DNA molecule randomly (c) cuts the DNA molecule at specific sites (d) restricts the synthesis of DNA inside the nucleus.
Last Answer : (c) cuts the DNA molecule at specific sites
Description : Following statements describe the characteristics of the enzyme restriction endonuclease. Identify the incorrect statement. (a) The enzyme recognises a specific palindromic nucleotide sequence in the DNA. (b) ... (d) The enzyme cuts the sugar-phosphate backbone at specific sites on each strand.
Last Answer : (c) The enzyme binds DNA at specific sites and cuts only one of the two strands.
Description : Which of the following reunites the exon segments after RNA splicing? (a) RNA polymerase (b) RNA primase (c) RNA ligase (d) RNA proteose
Last Answer : a) RNA polymerase
Description : Which of the following reunites the exon segments after RNA splicing? (a) RNA polymerase (b) RNA primase (c) RNA ligase (d) RNA proteoses
Last Answer : (c) RNA ligase
Description : Role of restriction enzymes and DNA ligase -Biology
Description : Restriction endonucleases are enzymes which (a) make cuts at specific positions within the DNA molecule (b) recognize a specific nucleotide sequence for binding of DNA ligase (c) restrict the action of the enzyme DNA polymerase (d) remove nucleotides from the ends of the DNA molecule
Last Answer : (a) make cuts at specific positions within the DNA molecule
Description : How are restriction enzymes used to make recombinant DNA? -Biology
Description : A foreign DNA and plasmid cut by the same restriction endonculease can be joined to form a recombinant plasmid using
Last Answer : A foreign DNA and plasmid cut by the same restriction endonculease can be joined to form a recombinant ... B. Ligase C. Eco RI D. Taq polymerase
Description : Without restriction endonucleases, it would be very difficult to a. Force Plasmids into Bacteria. b. Chemically Open Dna Molecules. c. Replicate Dna In A Recombinant Cell. d. Bring About Mutations In Bacteria.
Last Answer : b. Chemically Open Dna Molecules.
Description : What determines where a restriction endonuclease cuts? -Biology
Description : Function of restriction endonuclease in bacteria -Biology
Description : What is restriction endonuclease used for? -Biology
Description : There is a restriction endonuclease called EcoRI. What does “co” part in it stand for? (a) colon (b) coelom (c) coenzyme (d) coli
Last Answer : (d) coli
Description : Which of the following is a restriction endonuclease? (a) DNase I (b) RNase (c) Hind II (d) Protease
Last Answer : (c) Hind II
Description : Which complementary sequence would NOT be recognized by a restriction endonucLease? a. GAATTC CTTAAG b. AAGCTT TTCGAA c. GTCGAC CAGCTG d. AATTCCTTAAGG
Last Answer : d. AATTCCTTAAGG
Description : Which of the following statement is correct? A- The size and sequence of introns can be deduced from the cDNA sequence B- Restriction endonuclease can cleave ss and dsDNA both C- Restriction ... sequenceof a gene D- Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide
Last Answer : Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide
Description : Which of the following statement is correct? A- The size and sequence of introns can be deduced from the cDNA sequence B- .Restriction endonuclease can cleave ss and dsDNA both C- Restriction ... of a Gene D- Amino acid sequence of a protein can be deduced from corresponding cDNA nucleotide
Description : Choose the correct pair from the following. (a) Ligases - Join the two DNA molecules (b) Polymerases - Break the DNA into fragments (c) Nucleases - Separate the two strands of DNA (d) Exonucleases - Make cuts at specific positions within DNA
Last Answer : (a) Ligases - Join the two DNA molecules
Description : What is the role of the enzyme DNA primase?
Last Answer : Primase is an enzyme that synthesizes short RNA sequences called primers which serve as a starting point for DNA synthesis. Since primase produces RNA molecules, the enzyme is a type of RNA ... strand of nucleotides. Therefore, primase serves to prime and lay a foundation for DNA synthesis.
Description : In addition to Taq polymerase, polymerase chain reaction requires all of the following except (A) A template DNA (B) Deoxyribonucleoside triphosphates (C) Primers (D) Primase
Description : Primase activity is present in (A) DNA polymerase II (B) DNA polymerase α (C) DNA polymerase β (D) DNA polymerase δ
Last Answer : Answer : B
Description : RNA primer is formed by the enzyme: (A) Ribonuclease (B) Primase (C) DNA polymerase I (D) DNA polymerase III
Description : The enzyme which catalyzes the unwinding of DNA helix during replication is: (1) Primase (2) Topoisomerase (3) Helicase (4) DNA polymerase
Last Answer : (3) Helicase Explanation: A type of helicase enzyme called DNA helicase catalyzes the unwinding of the DNA helix at the time of replication. It uses the energy released through hydrolysis of ATP ( ... The enzyme is needed to separate the two strands of DNA also during transcription and DNA repair.
Description : The enzyme which catalyzes the unwinding of DNA helix during replication is: (1) Primase (2) Topoisomerase (3) Helicase (4) DNApolymerase
Last Answer : C option is the answer
Description : The spherical body made up of proteins; rRNAs and r DNAs is found inside the nucleus. It is known as?
Last Answer : Nucleolus
Description : Choking in case of pipe flow means that a (A) Specified mass flow rate cannot be achieved (B) Valve is closed in the line (C) Restriction in flow cross-section area occurs (D) None of these
Last Answer : (B) Valve is closed in the line
Description : (3) reflection of light due to the presence of free electrons Explanation: Lustre (or luster) is the way light interacts with the surface of a crystal, rock, or mineral. The word ... conductivity, and high density. Typically they are malleable and ductile, deforming -under stress without cleaving.
Last Answer : Cloud is a colloidal dispersion of – (1) Air in a dispersion medium of water (2) Fog in a dispersion medium of water (3) Mist in a dispersion medium of air (4) Water drops in a dispersion medium of air
Description : What is the basic fiber cleaving technique for preparing optical fibers for coupling?
Last Answer : Score-and-break.
Description : What is the role of the DNA ligase in DNA replication?
Last Answer : It joins Okazaki fragments
Description : $ The enzymes which joins short pieces DNA in the laggins strand is called ligase. ! Lagging strand and leading strand are formed due to replication f
Last Answer : $ The enzymes which joins short pieces DNA in the laggins strand is called ligase. ! Lagging strand and ... wrong D. If both As and R are wrong.
Description : What is the role of the DNA ligase in DNA replication apex?
Last Answer : It ties the chunks of DNA together.
Description : In the biosynthesis of c-DNA, the joining enzyme ligase requires (A) GTP (B) ATP (C) CTP (D) UTP
Description : DNA ligase of E. coli requires which of the following co-factors? (A) FAD (B) NAD+ (C) NADP+ (D) NADH
Description : Xeroderma pigmentosum results from a defect in (A) uvr ABC excinuclease (B) DNA polymerase I (C) DNA ligase (D) All of these
Last Answer : Answer : A
Description : Damage to DNA caused by ultraviolet radiation can be repaired by (A) uvr ABC excinuclease (B) DNA polymerase I (C) DNA ligase (D) All of these