Description : The initiation site for transcription is recognized by (A) α−Subunit of DNA-dependent RNA polymerase (B) β−Subunit of DNA-dependent RNA polymerase (C) Sigma factor (D) Rho factor
Last Answer : Answer : C
Description : During transcription holoenzyme RNA polymerase binds to a DNA sequence and the DNA assumes a saddle like structure at that point. What is that sequence called? (a) AAAT box (b) TATA box (c) GGTT box (d) CAAT box
Last Answer : (c) GGTT box
Last Answer : (b) TATA box
Description : In the process of transcription in bacterial cells (A) Initiation requires rho protein (B) RNA polymerase incorporates methylated bases in correct sequence (C) Both the sigma unit and core ... RNA polymerase are required for accurate promotor site binding (D) Primase is necessary for initiation
Description : After termination of the synthesis of RNA molecule, the core enzymes separate from the DNA template. The core enzymes then recognize a promoter at which the synthesis of a new RNA molecule commences, with the assistance of (A) Rho (ρ) factor (B) δ factor (C) β factor (D) σ factor
Last Answer : Answer : D
Description : The ‘rho’ (ρ) factor is involved (A) To increase the rate of RNA synthesis (B) In binding catabolite repressor to the promoter region (C) In proper termination of transcription (D) To allow proper initiation of transcriptide
Description : Lactose or its analogues act as positive regulators of lac operon by (A) Attaching to i gene and preventing its expression (B) Increasing the synthesis of catabolite gene activator protein (C) ... holoenzyme (D) Binding to repressor subunits so that the repressor cannot attach to the operator locus
Description : Binding of RNA polymerase holoenzyme to the promoter region of lac operon is facilitated by (A) Catabolite gene activator protein (CAP) (B) cAMP (C) CAP-cAMP complex (D) None of these
Description : RNA polymerase holoenzyme binds to lac operon at the following site: (A) i gene (B) z gene (C) Operator locus (D) Promoter region
Description : Ribonucleotides of RNA primer are replaced by deoxyribonucleotides by the enzyme: (A) DNA polymerase I (B) DNA polymerase II (C) DNA polymerase III holoenzyme (D) All of these
Last Answer : Answer : A
Description : Deoxyribonucleotides are added to RNA primer by (A) DNA polymerase I (B) DNA polymerase II (C) DNA polymerase III holoenzyme (D) All of these
Description : The termination site for transcription is recognized by (A) α−Subunit of DNA-dependent RNA polymerase (B) β−Subunit of DNA-dependent RNA polymerase (C) Sigma factor (D) Rho factor
Description : α-Amanitin inhibits (A) DNA polymerase II of prokaryotes (B) DNA polymerase α of eukaryotes (C) RNA polymerase II of eukaryotes (D) RNA-dependent DNA polymerase
Description : DNA-dependent RNA polymerase catalyzes transcription on one strand of the DNA which is called the
Last Answer : DNA-dependent RNA polymerase catalyzes transcription on one strand of the DNA which is called the ... Antistrand C. Template strand D. Coding strand
Description : All of the following statements about eukaryotic promoters are true except (A) They may be located upstream or down stream from the structural gene (B) They have two consensus sequences ( ... (D) Mutations in promoter region can decrease the efficiency of transcription of the structural gene
Description : All of the following statements about bacterial promoters are true except (A) They are smaller than eukaryotic promoters (B) They have two consensus sequences upstream from the transcription star site (C) ... is the site for attachment of RNA polymerase (D) TATA box has a high melting temperature
Description : The function of a repressor protein in an operon system is to prevent synthesis by binding to (A) The ribosome (B) A specific region of the operon preventing transcription of structural genes (C) The RNA polymerase (D) A specific region of the mRNA preventing translation to protein
Last Answer : Answer : B
Description : An important step in protein synthesis is transcription. Which of the following statement(s) is/are true concerning this process? a. The first step in gene transcription involves separating the double helix ... nucleus to the cytoplasm d. Only one protein can be produced from an initial mRNA strand
Last Answer : Answer: c Transcription of a gene begins at an initiation site associated with a specific DNA sequence, termed a promoter region. After binding to DNA, the RNA polymerase opens up a short ... different proteins from the same gene. mRNA is exported from the nucleus only after processing is complete
Description : During transcription, the DNA site at which RNA polymerase binds is called (a) promoter (b) regulator (c) receptor (d) enhancer.
Last Answer : d) enhancer
Description : DNA-dependent RNA polymerase catalyses transcription on one strand of the DNA which is called the (a) template strand (b) coding strand (c) alpha strand (d) antistrand
Last Answer : (a) template strand
Description : Name the enzyme that facilitates opening of DNA helix during transcription. (a) DNA ligase (b) DNA helicase (c) DNA polymerase (d) RNA polymerase
Last Answer : b) DNA helicase
Last Answer : (a) promoter
Description : DNA-dependent RNA polymerase catalyses transcription on one strand of the DNA which is called the (a) template strand (b) coding strand (c) alpha strand (d) antistrand.
Description : Select the incorrect statement out of the five given below about lac operon when Lactose is present in the medium. a) Gene - A gets transcribed into mRNA which produces β-galactoside permease b) ... polymerase transcribe Z-gene, Y-gene and A-gene e) Allolactose is the inducer of lac operon
Last Answer : Gene – A gets transcribed into mRNA which produces β-galactoside permease b) Inducer-Repressor complex is formed
Description : Termination of the synthesis of the RNA molecule is signaled by a sequence in the template strand of the DNA molecule, a signal that is recognized by a termination protein, the (A) Rho (ρ) factor (B) σ factor (C) δ factor (D) ε factor
Description : DNA polymerase III holoenzyme possesses (A) Polymerase activity (B) 3’→5’ Exonuclease activity (C) 5’→3’ Exonuclease and polymerase activities (D) 3’→5’ Exonuclease and polymerase activities
Description : Negative supercoils are introduced in DNA by (A) Helicase (B) DNA ligase (C) DNA gyrase (D) DNA polymerase III holoenzyme
Description : The expression for the loss tangent is given by a) σ/ωε b) ωε/σ c) σ/ω d) ω/ε
Last Answer : a) σ/ωε
Description : In eukaryotic cells (A) Formylated tRNA is important for initiation of translation (B) Cyclohexamide blocks elongation during translation (C) Cytosolic ribosomes are smaller than those found in prokaryotes (D) Erythromycin inhibits elongation during translation
Description : During translation initiation in prokaryotes, a GTP molecule is needed in (a) formation of formyl-met-tRNA (b) binding of 30S subunit of ribosome with mRNA (c) association of 30S mRNA with formyl-met- tRNA (d) association of 50S subunit of ribosome with initiation complex.
Last Answer : (c) association of 30S mRNA with formyl-met- tRNA
Description : .During translation initiation in prokaryotes, a GTP molecule is needed in (a) formation of formyl-met-tRNA (b) binding of 30S subunit of ribosome with mRNA (c) association of 30S mRNA with formyl-met- tRNA (d) association of 50S subunit of ribosome with initiation complex.
Description : Rifampicin inhibits (A) Unwinding of DNA (B) Initiation of replication (C) Initiation of translation (D) Initiation of transcription
Description : .In protein synthesis, the polymerisation of amino acids involves three steps. Which one of the following is not involved in the polymerisation of protein ? (a) Termination (b) Initiation (c) Elongation (d) Transcription
Last Answer : (a) Termination
Description : In protein synthesis, the polymerisation of amino acids involves three steps. Which one of the following is not involved in the polymerisation of protein ? (a) Termination (b) Initiation (c) Elongation (d) Transcription
Last Answer : Transcription
Description : Synthesis of RNA molecule is terminated by a signal which is recognised by (A) α-factor (B) β-factor (C) δ-factor (D) ρ
Description : The fatty acids containing even number and odd number of carbon atoms as well as the unsaturated fatty acids are oxidized by (A) α-oxidation (B) β-oxidation (C) ω-oxidation (D) All of these
Description : Fatty acids are oxidized by (A) α -oxidation (B) β -oxidation (C) ω -oxidation (D) All of these
Description : The expression for phase constant is given by a) Phase constant β = ωμε b) Phase constant ω = με c) Phase constant β = ω√(με) d) Phase constant β = 1/ωμε
Last Answer : c) Phase constant β = ω√(με)
Description : Ciprofloxacin inhibits the synthesis of (A) DNA in prokaryotes (B) DNA in prokaryotes and eukaryotes (C) RNA in prokaryotes (D) RNA in prokaryotes and eukaryotes
Description : Translocase is an enzyme required in the process of (A) DNA replication (B) RNA synthesis (C) Initiation of protein synthesis (D) Elongation of peptides
Description : Which binding is inhibited by pactamycin? A- Aminoacyl-tRNA to the A-site of 30S subunit B- Initiator-tRNA to 30S/40S initiation complexes C- Peptidyl t-RNA to the 50S subunit D- Formation of peptide bond in P site
Last Answer : Initiator-tRNA to 30S/40S initiation complexes
Description : Co-factor (prosthetic group) is a part of holoenzyme. It is (a) loosely attached organic part (b) loosely attached inorganic part (c) accessory non-protein substance attached firmly (d) none of these.
Last Answer : (c) accessory non-protein substance attached firmly
Description : Which of the following statements is correct with reference to enzymes? (a) Holoenzyme = Apoenzyme + Coenzyme (b) Coenzyme = Apoenzyme + Holoenzyme (c) Holoenzyme = Coenzyme + Co-factor (d) Apoenzyme = Holoenzyme + Coenzyme
Last Answer : a) Holoenzyme = Apoenzyme + Coenzyme
Description : Which one of the following statements is correct , with reference to enzymes ? (1) Holoenzyme = Apoenzyme + Coenzyme (2) Coenzyme = Apoenzyme + Holoenzyme (3) Holoenzyme = Coenzyme + Co-factor (4) Apoenzyme = Holoenzyme + Coenzyme
Last Answer : (1) Holoenzyme = Apoenzyme + Coenzyme
Description : The mammalian DNA polymerase involved in error correction is (A) DNA polymerase α (B) DNA polymerase β (C) DNA polymerase γ (D) DNA polymerase δ
Description : Primase activity is present in (A) DNA polymerase II (B) DNA polymerase α (C) DNA polymerase β (D) DNA polymerase δ
Description : Replication of nuclear DNA in mammals is catalysed by (A) DNA polymerase α (B) DNA polymerase β (C) DNA polymerase γ (D) DNA polymerase III
Description : .Gene regulation governing lactose operon of E.coli that involves the lac I gene product is (a) negative and repressible because repressor protein prevents transcription (b) feedback inhibition ... be induced by lactose (d) negative and inducible because repressor protein prevents transcription.
Last Answer : (d) negative and inducible because repressor protein prevents transcription.
Description : Gene regulation governing lactose operon of E.coli that involves the lac I gene product is (a) negative and repressible because repressor protein prevents transcription (b) feedback inhibition ... be induced by lactose (d) negative and inducible because repressor protein prevents transcription.
Description : What is the difference between DNA and RNA polymerase?
Last Answer : DNA polymerase is responsible for synthesizing new strands of DNA during DNA replication, while RNA polymerase is responsible for synthesizing mRNA during transcription.