Description : DNA polymerase I possesses (A) Polymerase activity (B) 3’→5’ Exonuclease activity (C) 5’→3’ Exonuclease activity (D) All of these
Last Answer : Answer : D
Description : 3’→5’ Exonuclease activity of DNA polymerase I (A) Removes ribonucleotides (B) Adds deoxyribonucleotides (C) Corrects errors in replication (D) Hydrolyses DNA into mononucleotides
Last Answer : Answer : C
Description : All of the following statements about RNA-dependent DNA polymerase are true except: (A) It synthesizes DNA using RNA as a template (B) It is also known as reverse transcriptase (C) It synthesizes DNA in 5’→3’ direction (D) It is present in all the viruses
Description : Negative supercoils are introduced in DNA by (A) Helicase (B) DNA ligase (C) DNA gyrase (D) DNA polymerase III holoenzyme
Description : Ribonucleotides of RNA primer are replaced by deoxyribonucleotides by the enzyme: (A) DNA polymerase I (B) DNA polymerase II (C) DNA polymerase III holoenzyme (D) All of these
Last Answer : Answer : A
Description : Deoxyribonucleotides are added to RNA primer by (A) DNA polymerase I (B) DNA polymerase II (C) DNA polymerase III holoenzyme (D) All of these
Description : What is exonuclease activity of DNA polymerase? -Biology
Last Answer : answer:
Description : During transcription holoenzyme RNA polymerase binds to a DNA sequence and the DNA assumes a saddle like structure at that point. What is that sequence called? (a) AAAT box (b) TATA box (c) GGTT box (d) CAAT box
Last Answer : (c) GGTT box
Last Answer : (b) TATA box
Description : Lactose or its analogues act as positive regulators of lac operon by (A) Attaching to i gene and preventing its expression (B) Increasing the synthesis of catabolite gene activator protein (C) ... holoenzyme (D) Binding to repressor subunits so that the repressor cannot attach to the operator locus
Description : Binding of RNA polymerase holoenzyme to the promoter region of lac operon is facilitated by (A) Catabolite gene activator protein (CAP) (B) cAMP (C) CAP-cAMP complex (D) None of these
Description : RNA polymerase holoenzyme binds to lac operon at the following site: (A) i gene (B) z gene (C) Operator locus (D) Promoter region
Description : The transcription initiation factor associated with the RNA polymerase holoenzyme in prokaryotes is (a) β (b) ω (c) σ (d) αI
Last Answer : c) σ (
Description : Which one of the following statements correctly describes eukaryotic DNA? (A) If uses DNA polymerase with nuclease activities (B) It is replicated bidirectionally at many points (C) It contains no repetitive DNA (D) It is nonlinear
Description : Primase activity is present in (A) DNA polymerase II (B) DNA polymerase α (C) DNA polymerase β (D) DNA polymerase δ
Last Answer : Answer : B
Description : DNA-dependent RNA polymerase requires the following for its catalytic activity: (A) Mg++ (B) Mn++ (C) Both (A) and (B) (D) None of these
Description : Reverse transcriptase activity is present in the eukaryotic: (A) DNA polymerase α (B) DNA polymerase γ (C) Telomerase (D) DNA polymerase II
Description : An organic substance bound to an enzyme and essential for the activity of enzyme is called (A) Holoenzyme (B) Apoenzyme (C) Coenzyme (D) Isoenzyme
Description : RNA directed DNA polymerase is (A) Replicase (B) Transcriptase (C) Reversetranscriptase (D) Polymerase–III
Description : DNA directed RNA polymerase is (A) Replicase (B) Transcriptase (C) Reverse transcriptase (D) Polymerase III
Description : Ciprofloxacin inhibits (A) DNA topisomerase II (B) DNA polymerase I (C) DNA polymerase III (D) DNA gyrase
Description : Replication of nuclear DNA in mammals is catalysed by (A) DNA polymerase α (B) DNA polymerase β (C) DNA polymerase γ (D) DNA polymerase III
Description : RNA primer is formed by the enzyme: (A) Ribonuclease (B) Primase (C) DNA polymerase I (D) DNA polymerase III
Description : What is exonuclease activity?
Last Answer : What is the answer ?
Description : Which of the following cut the DNA from specific places? (a) E.coli restriction endonuclease I (b) Ligase (c) Exonuclease (d) Alkaline phosphate
Last Answer : (a) E.coli restriction endonuclease I
Description : 1. Bacteria protect themselves from viruses by fragmenting viral DNA with a. Ligase b. Endonuclease c. Exonuclease d. Gyrase
Last Answer : Ans: Endonuclease.
Description : eIF-4 A possesses (A) ATPase activity (B) GTPase activity (C) Helicase activity (D) None of these
Description : All the following statements about ceruloplasmin are correct except (A) It is a copper-containing protein (B) It possesses oxidase activity (C) It is synthesised in intestinal mucosa (D) Its plasma level is decreased inWilson’s disease
Description : The mechanism of antibacterial action of tetracycline involves (a) Binding to a component of the 50S ribosomal subunit (b) Inhibition of translocase activity (c) Blockade of binding of ... (d) Selective inhibition of ribosomal peptidyl transferases (e) Inhibition of DNA-dependent RNA polymerase
Last Answer : Ans: C
Description : What is the function of DNA polymerase III? -Biology
Description : What characteristics of DNA polymerase III make it an ideal enzyme for replicating the whole genome?
Last Answer : The key features of DNA polymerase III are its catalytic potency, its fidelity, and its processivity. It is able to catalyze the addition of ~103 bases per second, compared to only about 10 per ... to dissociate from the template strand after synthesis of short (tens of bases) stretches of DNA.
Description : Match the organism with its use in biotechnology. (A) Bacillus (i) Cloning vector thuringiensis (B) Thermus (ii) Construction of first aquaticus rDNA molecule (C) Agrobacterium (iii)DNA polymerase tumefaciens (D) Salmonella (iv) Cry proteins ... (ii) (c) (iii) (ii) (iv) (i) (d) (iii) (iv) (i) (ii)
Last Answer : (b) (iv) (iii) (i) (ii)
Description : A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using (a) EcoRI (b) Taq polymerase (c) polymerase III (d) ligase.
Last Answer : (d) ligase.
Description : The enzyme adds complementary bases to the DNA template strand during replication. a. Ligase b. Helicase c. DNA polymerase III d. RNA polymerase
Last Answer : d. RNA polymerase
Description : Template-directed DNA synthesis occurs in all the following except (A) The replication fork (B) Polymerase chain reaction (C) Growth of RNA tumor viruses (D) Expression of oncogenes
Description : The binding of prokaryotic DNA dependent RNA polymerase to promoter sits of genes is inhibited by the antibiotic: (A) Streptomycin (B) Rifamcin (C) Aueromycin (D) Puromycin
Description : Another name for reverse transcriptase is (A) DNA dependent DNA polymerase (B) DNA dependent RNA polymerase (C) RNA dependent DNA polymerase (D) RNA dependent RNA polymerase
Description : Template-directed DNA synthesis occurs in all the following except (A) The replication fork (B) Polymerase chain reaction (C) Growth of RNA tumor viruses (D) Expression of oneogenes
Description : The antibiotic which inhibits DNA dependent RNA polymerase is (A) Mitomycin C (B) Actinomycin d (C) Streptomycin (D) Puromycin
Description : In addition to Taq polymerase, polymerase chain reaction requires all of the following except (A) A template DNA (B) Deoxyribonucleoside triphosphates (C) Primers (D) Primase
Description : Optimum temperature of DNA polymerase of T. aquaticus is (A) 30°C (B) 37°C (C) 54°C (D) 72°C
Description : The DNA polymerase commonly used in polymerase chain reaction is obtained from (A) E. coli (B) Yeast (C) T.aquaticus (D) Eukaryotes
Description : Polymerase chain reaction can rapidly amplify DNA sequences of the size (A) Upto 10 kilobases (B) Upto 45 kilobases (C) Upto 100 kilobases(D) Upto 1,000 kilobases
Description : Myeloma cells and lymphocytes can be fused by using (A) Calcium chloride (B) Ethidium bromide (C) Polyethylene glycol (D) DNA polymerase
Description : Xeroderma pigmentosum results from a defect in (A) uvr ABC excinuclease (B) DNA polymerase I (C) DNA ligase (D) All of these
Description : Damage to DNA caused by ultraviolet radiation can be repaired by (A) uvr ABC excinuclease (B) DNA polymerase I (C) DNA ligase (D) All of these
Description : α-Amanitin inhibits (A) DNA polymerase II of prokaryotes (B) DNA polymerase α of eukaryotes (C) RNA polymerase II of eukaryotes (D) RNA-dependent DNA polymerase
Description : DNA contains some palindromic sequences which (A) Mark the site for the formation of replication forks (B) Direct DNA polymerase to turn back to replicate the other strand (C) Are recognized by restriction enzymes (D) Are found only in bacterial DNA
Description : The mammalian DNA polymerase involved in error correction is (A) DNA polymerase α (B) DNA polymerase β (C) DNA polymerase γ (D) DNA polymerase δ
Description : Mammalian DNA polymerase γ is located in (A) Nucleus (B) Nucleolus (C) Mitochondria (D) Cytosol