In the process of transcription in bacterial cells (A) Initiation requires rho protein (B) RNA polymerase incorporates methylated bases in correct sequence (C) Both the sigma unit and core enzymes of RNA polymerase are required for accurate promotor site binding (D) Primase is necessary for initiation

In the process of transcription in bacterial cells (A) Initiation requires rho protein (B) RNA polymerase incorporates methylated bases in correct sequence (C) Both the sigma unit and core enzymes of RNA polymerase are required for accurate promotor site binding (D) Primase is necessary for initiation
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The initiation site for transcription is recognized by (A) α−Subunit of DNA-dependent RNA polymerase (B) β−Subunit of DNA-dependent RNA polymerase (C) Sigma factor (D) Rho factor

Description : The initiation site for transcription is recognized by (A) α−Subunit of DNA-dependent RNA polymerase (B) β−Subunit of DNA-dependent RNA polymerase (C) Sigma factor (D) Rho factor

Last Answer : Answer : C

The termination site for transcription is recognized by (A) α−Subunit of DNA-dependent RNA polymerase (B) β−Subunit of DNA-dependent RNA polymerase (C) Sigma factor (D) Rho factor

Description : The termination site for transcription is recognized by (A) α−Subunit of DNA-dependent RNA polymerase (B) β−Subunit of DNA-dependent RNA polymerase (C) Sigma factor (D) Rho factor

Last Answer : Answer : D

The ‘rho’ (ρ) factor is involved (A) To increase the rate of RNA synthesis (B) In binding catabolite repressor to the promoter region (C) In proper termination of transcription (D) To allow proper initiation of transcriptide

Description : The ‘rho’ (ρ) factor is involved (A) To increase the rate of RNA synthesis (B) In binding catabolite repressor to the promoter region (C) In proper termination of transcription (D) To allow proper initiation of transcriptide

Last Answer : Answer : C

Sigma and Rho factors are required for (A) Replication (B) Transcription (C) Translation (D) Polymerisation

Description : Sigma and Rho factors are required for (A) Replication (B) Transcription (C) Translation (D) Polymerisation

Last Answer : Answer : C

The function of a repressor protein in an operon system is to prevent synthesis by binding to (A) The ribosome (B) A specific region of the operon preventing transcription of structural genes (C) The RNA polymerase (D) A specific region of the mRNA preventing translation to protein

Description : The function of a repressor protein in an operon system is to prevent synthesis by binding to (A) The ribosome (B) A specific region of the operon preventing transcription of structural genes (C) The RNA polymerase (D) A specific region of the mRNA preventing translation to protein

Last Answer : Answer : B

All of the following statements about bacterial promoters are true except (A) They are smaller than eukaryotic promoters (B) They have two consensus sequences upstream from the transcription star site (C) TATA box is the site for attachment of RNA polymerase (D) TATA box has a high melting temperature

Description : All of the following statements about bacterial promoters are true except (A) They are smaller than eukaryotic promoters (B) They have two consensus sequences upstream from the transcription star site (C) ... is the site for attachment of RNA polymerase (D) TATA box has a high melting temperature

Last Answer : Answer : D

After termination of the synthesis of RNA molecule, the core enzymes separate from the DNA template. The core enzymes then recognize a promoter at which the synthesis of a new RNA molecule commences, with the assistance of (A) Rho (ρ) factor (B) δ factor (C) β factor (D) σ factor

Description : After termination of the synthesis of RNA molecule, the core enzymes separate from the DNA template. The core enzymes then recognize a promoter at which the synthesis of a new RNA molecule commences, with the assistance of (A) Rho (ρ) factor (B) δ factor (C) β factor (D) σ factor

Last Answer : Answer : D

The transcription initiation factor associated with the RNA polymerase holoenzyme in prokaryotes is (a) β (b) ω (c) σ (d) αI

Description : The transcription initiation factor associated with the RNA polymerase holoenzyme in prokaryotes is (a) β (b) ω (c) σ (d) αI

Last Answer : c) σ (

Termination of the synthesis of the RNA molecule is signaled by a sequence in the template strand of the DNA molecule, a signal that is recognized by a termination protein, the (A) Rho (ρ) factor (B) σ factor (C) δ factor (D) ε factor

Description : Termination of the synthesis of the RNA molecule is signaled by a sequence in the template strand of the DNA molecule, a signal that is recognized by a termination protein, the (A) Rho (ρ) factor (B) σ factor (C) δ factor (D) ε factor

Last Answer : Answer : A

All of the following statements about eukaryotic promoters are true except (A) They may be located upstream or down stream from the structural gene (B) They have two consensus sequences (C) One consensus sequence binds RNA polymerase (D) Mutations in promoter region can decrease the efficiency of transcription of the structural gene

Description : All of the following statements about eukaryotic promoters are true except (A) They may be located upstream or down stream from the structural gene (B) They have two consensus sequences ( ... (D) Mutations in promoter region can decrease the efficiency of transcription of the structural gene

Last Answer : Answer : A

RNA primer is formed by the enzyme: (A) Ribonuclease (B) Primase (C) DNA polymerase I (D) DNA polymerase III

Description : RNA primer is formed by the enzyme: (A) Ribonuclease (B) Primase (C) DNA polymerase I (D) DNA polymerase III

Last Answer : Answer : B

In addition to Taq polymerase, polymerase chain reaction requires all of the following except (A) A template DNA (B) Deoxyribonucleoside triphosphates (C) Primers (D) Primase

Description : In addition to Taq polymerase, polymerase chain reaction requires all of the following except (A) A template DNA (B) Deoxyribonucleoside triphosphates (C) Primers (D) Primase

Last Answer : Answer : D

Lactose or its analogues act as positive regulators of lac operon by (A) Attaching to i gene and preventing its expression (B) Increasing the synthesis of catabolite gene activator protein (C) Attaching to promoter region and facilitating the binding of RNA polymerase holoenzyme (D) Binding to repressor subunits so that the repressor cannot attach to the operator locus

Description : Lactose or its analogues act as positive regulators of lac operon by (A) Attaching to i gene and preventing its expression (B) Increasing the synthesis of catabolite gene activator protein (C) ... holoenzyme (D) Binding to repressor subunits so that the repressor cannot attach to the operator locus

Last Answer : Answer : D

Binding of RNA polymerase holoenzyme to the promoter region of lac operon is facilitated by (A) Catabolite gene activator protein (CAP) (B) cAMP (C) CAP-cAMP complex (D) None of these

Description : Binding of RNA polymerase holoenzyme to the promoter region of lac operon is facilitated by (A) Catabolite gene activator protein (CAP) (B) cAMP (C) CAP-cAMP complex (D) None of these

Last Answer : Answer : C

An important step in protein synthesis is transcription. Which of the following statement(s) is/are true concerning this process? a. The first step in gene transcription involves separating the double helix of DNA by an enzyme known as DNA polymerase b. The initial product of DNA transcription is called heterogeneous nuclear RNA which codes directly for proteins c. After processing is complete, the mRNA is exported from the nucleus to the cytoplasm d. Only one protein can be produced from an initial mRNA strand

Description : An important step in protein synthesis is transcription. Which of the following statement(s) is/are true concerning this process? a. The first step in gene transcription involves separating the double helix ... nucleus to the cytoplasm d. Only one protein can be produced from an initial mRNA strand

Last Answer : Answer: c Transcription of a gene begins at an initiation site associated with a specific DNA sequence, termed a promoter region. After binding to DNA, the RNA polymerase opens up a short ... different proteins from the same gene. mRNA is exported from the nucleus only after processing is complete

The mechanism of antibacterial action of tetracycline involves (a) Binding to a component of the 50S ribosomal subunit (b) Inhibition of translocase activity (c) Blockade of binding of aminoacyl – tRNA to bacterial ribosomes (d) Selective inhibition of ribosomal peptidyl transferases (e) Inhibition of DNA–dependent RNA polymerase

Description : The mechanism of antibacterial action of tetracycline involves (a) Binding to a component of the 50S ribosomal subunit (b) Inhibition of translocase activity (c) Blockade of binding of ... (d) Selective inhibition of ribosomal peptidyl transferases (e) Inhibition of DNA-dependent RNA polymerase

Last Answer : Ans: C

The correct statement concerning RNA and DNA polymerases is (A) RNA polymerase use nucleoside diphosphates (B) RNA polymerase require primers and add bases at 5’ end of the growing polynucleotide chain (C) DNA polymerases can add nucleotides at both ends of the chain (D) All RNA and DNA polymerases can add nucleotides only at the 3’ end of the growing polynucleotide chain

Description : The correct statement concerning RNA and DNA polymerases is (A) RNA polymerase use nucleoside diphosphates (B) RNA polymerase require primers and add bases at 5' end of the growing polynucleotide ... and DNA polymerases can add nucleotides only at the 3' end of the growing polynucleotide chain

Last Answer : Answer : D

During transcription holoenzyme RNA polymerase binds to a DNA sequence and the DNA assumes a saddle like structure at that point. What is that sequence called? (a) AAAT box (b) TATA box (c) GGTT box (d) CAAT box

Description : During transcription holoenzyme RNA polymerase binds to a DNA sequence and the DNA assumes a saddle like structure at that point. What is that sequence called? (a) AAAT box (b) TATA box (c) GGTT box (d) CAAT box

Last Answer : (c) GGTT box

During transcription holoenzyme RNA polymerase binds to a DNA sequence and the DNA assumes a saddle like structure at that point. What is that sequence called? (a) AAAT box (b) TATA box (c) GGTT box (d) CAAT box

Description : During transcription holoenzyme RNA polymerase binds to a DNA sequence and the DNA assumes a saddle like structure at that point. What is that sequence called? (a) AAAT box (b) TATA box (c) GGTT box (d) CAAT box

Last Answer : (b) TATA box

The region of DNA known as TATA BOX is the site for binding of (A) DNA polymerase (B) DNA topoisomerase (C) DNA dependent RNA polymerase (D) Polynucleotide phosphorylase

Description : The region of DNA known as TATA BOX is the site for binding of (A) DNA polymerase (B) DNA topoisomerase (C) DNA dependent RNA polymerase (D) Polynucleotide phosphorylase

Last Answer : Answer : C

Translocase is an enzyme required in the process of (A) DNA replication (B) RNA synthesis (C) Initiation of protein synthesis (D) Elongation of peptides

Description : Translocase is an enzyme required in the process of (A) DNA replication (B) RNA synthesis (C) Initiation of protein synthesis (D) Elongation of peptides

Last Answer : Answer : D

Which of the following reunites the exon segments after RNA splicing? (a) RNA polymerase (b) RNA primase (c) RNA ligase (d) RNA proteose

Description : Which of the following reunites the exon segments after RNA splicing? (a) RNA polymerase (b) RNA primase (c) RNA ligase (d) RNA proteose

Last Answer : a) RNA polymerase

Which of the following reunites the exon segments after RNA splicing? (a) RNA polymerase (b) RNA primase (c) RNA ligase (d) RNA proteoses

Description : Which of the following reunites the exon segments after RNA splicing? (a) RNA polymerase (b) RNA primase (c) RNA ligase (d) RNA proteoses

Last Answer : (c) RNA ligase

The removal of which enzyme affects the synthesis of hnRNA in eukaryotes a) RNA polymerase II b) RNA primase c) RNA polymerase III d) RNA polymerase I

Description : The removal of which enzyme affects the synthesis of hnRNA in eukaryotes a) RNA polymerase II b) RNA primase c) RNA polymerase III d) RNA polymerase I

Last Answer : a) RNA polymerase II

Which of the following enzymes are used to join bits of DNA? (a) Ligase (b) Primase (c) DNA polymerase (d) Endonuclease

Description : Which of the following enzymes are used to join bits of DNA? (a) Ligase (b) Primase (c) DNA polymerase (d) Endonuclease

Last Answer : a) Ligase

Initiation of protein synthesis begins with binding of (A) 40S ribosomal unit on mRNA (B) 60S ribosomal unit (C) Charging of tRNA with specific amino acid (D) Attachment of aminoacyl tRNA on mRNA

Description : Initiation of protein synthesis begins with binding of (A) 40S ribosomal unit on mRNA (B) 60S ribosomal unit (C) Charging of tRNA with specific amino acid (D) Attachment of aminoacyl tRNA on mRNA

Last Answer : Answer : A

Restriction endonucleases are enzymes which (a) make cuts at specific positions within the DNA molecule (b) recognize a specific nucleotide sequence for binding of DNA ligase (c) restrict the action of the enzyme DNA polymerase (d) remove nucleotides from the ends of the DNA molecule

Description : Restriction endonucleases are enzymes which (a) make cuts at specific positions within the DNA molecule (b) recognize a specific nucleotide sequence for binding of DNA ligase (c) restrict the action of the enzyme DNA polymerase (d) remove nucleotides from the ends of the DNA molecule

Last Answer : (a) make cuts at specific positions within the DNA molecule

DNA contains some palindromic sequences which (A) Mark the site for the formation of replication forks (B) Direct DNA polymerase to turn back to replicate the other strand (C) Are recognized by restriction enzymes (D) Are found only in bacterial DNA

Description : DNA contains some palindromic sequences which (A) Mark the site for the formation of replication forks (B) Direct DNA polymerase to turn back to replicate the other strand (C) Are recognized by restriction enzymes (D) Are found only in bacterial DNA

Last Answer : Answer : C

Which binding is inhibited by pactamycin? A- Aminoacyl-tRNA to the A-site of 30S subunit B- Initiator-tRNA to 30S/40S initiation complexes C- Peptidyl t-RNA to the 50S subunit D- Formation of peptide bond in P site

Description : Which binding is inhibited by pactamycin? A- Aminoacyl-tRNA to the A-site of 30S subunit B- Initiator-tRNA to 30S/40S initiation complexes C- Peptidyl t-RNA to the 50S subunit D- Formation of peptide bond in P site

Last Answer : Initiator-tRNA to 30S/40S initiation complexes

The ribosome binding site A- forms a stem-loop structure in the RNA B- is located upstream of the promoter sequence C- .is located immediately upstream of the start codon D- is more likely to be associated with an operon than with a gene encoding a single protein

Description : The ribosome binding site A- forms a stem-loop structure in the RNA B- is located upstream of the promoter sequence C- .is located immediately upstream of the start codon D- is more likely to be associated with an operon than with a gene encoding a single protein

Last Answer : .is located immediately upstream of the start codon

The rivosome binding site A- forms a sltem- loop structure in the RNA B- is located upstream of the promoter sequence C- is located immediately upstream of the start codon D- is more likely to be associated with an operon than with a gene encoding a single protein

Description : The rivosome binding site A- forms a sltem- loop structure in the RNA B- is located upstream of the promoter sequence C- is located immediately upstream of the start codon D- is more likely to be associated with an operon than with a gene encoding a single protein

Last Answer : is located immediately upstream of the start codon

The ribosome binding site A- forms a stem-loop structure in the RNA B- is located upstream of the promoter sequence C- .is located immediately upstream of the start codon D- is more likely to be associated with an operon than with a gene encoding a single protein

Description : The ribosome binding site A- forms a stem-loop structure in the RNA B- is located upstream of the promoter sequence C- .is located immediately upstream of the start codon D- is more likely to be associated with an operon than with a gene encoding a single protein

Last Answer : .is located immediately upstream of the start codon

Negative regulation of protein synthesis is accomplished by A- allosteric inhibition B- the binding of RNA polymerase to the promoter C- the binding of a repressor to the DNA D- the binding of a repressor to the RNA polymerase

Description : Negative regulation of protein synthesis is accomplished by A- allosteric inhibition B- the binding of RNA polymerase to the promoter C- the binding of a repressor to the DNA D- the binding of a repressor to the RNA polymerase

Last Answer : the binding of a repressor to the DNA

During transcription, the DNA site at which RNA polymerase binds is called (a) promoter (b) regulator (c) receptor (d) enhancer.

Description : During transcription, the DNA site at which RNA polymerase binds is called (a) promoter (b) regulator (c) receptor (d) enhancer.

Last Answer : d) enhancer

During transcription, the DNA site at which RNA polymerase binds is called (a) promoter (b) regulator (c) receptor (d) enhancer.

Description : During transcription, the DNA site at which RNA polymerase binds is called (a) promoter (b) regulator (c) receptor (d) enhancer.

Last Answer : (a) promoter

A consensus sequence on DNA, called TATA box, is the site for attachment of (A) RNA-dependent DNA polymerase (B) DNA-dependent RNA polymerase (C) DNA-dependent DNA polymerase (D) DNA topoisomerase II

Description : A consensus sequence on DNA, called TATA box, is the site for attachment of (A) RNA-dependent DNA polymerase (B) DNA-dependent RNA polymerase (C) DNA-dependent DNA polymerase (D) DNA topoisomerase II

Last Answer : Answer : B

All of the following statements about post-transcriptional processing of tRNA are true except (A) Introns of some tRNA precursors are removed (B) CCA is added at 3′ end (C) 7-Methylguanosine triphosphate cap is added at 5′ end (D) Some bases are methylated

Description : All of the following statements about post-transcriptional processing of tRNA are true except (A) Introns of some tRNA precursors are removed (B) CCA is added at 3′ end (C) 7-Methylguanosine triphosphate cap is added at 5′ end (D) Some bases are methylated

Last Answer : Answer : C

The binding of prokaryotic DNA dependent RNA polymerase to promoter sits of genes is inhibited by the antibiotic: (A) Streptomycin (B) Rifamcin (C) Aueromycin (D) Puromycin

Description : The binding of prokaryotic DNA dependent RNA polymerase to promoter sits of genes is inhibited by the antibiotic: (A) Streptomycin (B) Rifamcin (C) Aueromycin (D) Puromycin

Last Answer : Answer : B

The binding of prokaryotic DNA dependent RNA polymerase to promoter sites of genes is inhibited by the antibiotic: (A) Puromycin (B) Rifamycin (C) Terramycin (D) Streptomycin

Description : The binding of prokaryotic DNA dependent RNA polymerase to promoter sites of genes is inhibited by the antibiotic: (A) Puromycin (B) Rifamycin (C) Terramycin (D) Streptomycin

Last Answer : Answer : B

Unidirectional tandem gene arrays are significant in ________ a. Increase in recombinant gene product with increasing plasmid number. b. Increase in recombinant gene product without increasing plasmid number. c. Decrease in polymerase enzyme battery with increasing plasmid numbers. d. Decrease in strong promotor with increase in plasmid number

Description : Unidirectional tandem gene arrays are significant in ________ a. Increase in recombinant gene product with increasing plasmid number. b. Increase in recombinant gene product without increasing plasmid ... increasing plasmid numbers. d. Decrease in strong promotor with increase in plasmid number

Last Answer : b. Increase in recombinant gene product without increasing plasmid number.

DNA-dependent RNA polymerase requires the following for its catalytic activity: (A) Mg++ (B) Mn++ (C) Both (A) and (B) (D) None of these

Description : DNA-dependent RNA polymerase requires the following for its catalytic activity: (A) Mg++ (B) Mn++ (C) Both (A) and (B) (D) None of these

Last Answer : Answer : C

Primase activity is present in (A) DNA polymerase II (B) DNA polymerase α (C) DNA polymerase β (D) DNA polymerase δ

Description : Primase activity is present in (A) DNA polymerase II (B) DNA polymerase α (C) DNA polymerase β (D) DNA polymerase δ

Last Answer : Answer : B

In the process of transcription, the flow of genetic information is from (A) DNA to DNA (B) DNA to protein (C) RNA to protein (D) DNA to RNA

Description : In the process of transcription, the flow of genetic information is from (A) DNA to DNA (B) DNA to protein (C) RNA to protein (D) DNA to RNA

Last Answer : Answer : D

In the process of transcription, the flow of genetic information is from (A) DNA to DNA (B) DNA to protein (C) RNA to protein (D) DNA to RNA

Description : In the process of transcription, the flow of genetic information is from (A) DNA to DNA (B) DNA to protein (C) RNA to protein (D) DNA to RNA

Last Answer : Answer : D

All of the following are zinc-containing enzymes except (A) Acid Phosphatase (B) Alkaline Phosphatase (C) Carbonic anhydrase (D) RNA polymerase

Description : All of the following are zinc-containing enzymes except (A) Acid Phosphatase (B) Alkaline Phosphatase (C) Carbonic anhydrase (D) RNA polymerase

Last Answer : Answer : A

The enzyme adds complementary bases to the DNA template strand during replication. a. Ligase b. Helicase c. DNA polymerase III d. RNA polymerase

Description : The enzyme adds complementary bases to the DNA template strand during replication. a. Ligase b. Helicase c. DNA polymerase III d. RNA polymerase

Last Answer : d. RNA polymerase

DNA-dependent RNA polymerase catalyzes transcription on one strand of the DNA which is called the

Description : DNA-dependent RNA polymerase catalyzes transcription on one strand of the DNA which is called the

Last Answer : DNA-dependent RNA polymerase catalyzes transcription on one strand of the DNA which is called the ... Antistrand C. Template strand D. Coding strand

DNA-dependent RNA polymerase catalyses transcription on one strand of the DNA which is called the (a) template strand (b) coding strand (c) alpha strand (d) antistrand

Description : DNA-dependent RNA polymerase catalyses transcription on one strand of the DNA which is called the (a) template strand (b) coding strand (c) alpha strand (d) antistrand

Last Answer : (a) template strand

Name the enzyme that facilitates opening of DNA helix during transcription. (a) DNA ligase (b) DNA helicase (c) DNA polymerase (d) RNA polymerase

Description : Name the enzyme that facilitates opening of DNA helix during transcription. (a) DNA ligase (b) DNA helicase (c) DNA polymerase (d) RNA polymerase

Last Answer : b) DNA helicase

DNA-dependent RNA polymerase catalyses transcription on one strand of the DNA which is called the (a) template strand (b) coding strand (c) alpha strand (d) antistrand.

Description : DNA-dependent RNA polymerase catalyses transcription on one strand of the DNA which is called the (a) template strand (b) coding strand (c) alpha strand (d) antistrand.

Last Answer : (a) template strand