What is the use of biuret reaction? 

1 Answer

Answer :

This reaction can be used for qualitative identifi-

cation and quantitative estimation of proteins.

Related questions

Description : Biuret reaction is specific for (A) –CONH-linkages (B) –CSNH2 group (C) –(NH)NH2 group (D) All of these

Last Answer : Answer : A

Description : What is biuret reaction?

Last Answer : Cupric ions in alkaline medium form a violet colour with peptide bond nitrogen.

Description : What are the Reaction of amino acid using ninhydrin and biuret reagent?

Last Answer : What is the answer ?

Description : Pick out the correct statement. (A) Reaction of NH3 with HNO3 to produce (NH4 )2NO3 is endothermic (B) With increase in NH3 /CO2 ratio, urea yield decreases for a given temperature, pressure and total ... (an intermediate during urea manufacture) is toxic to seeds and animals (D) Both (B) and (C)

Last Answer : (B) With increase in NH3 /CO2 ratio, urea yield decreases for a given temperature, pressure and total feed rate

Description : A protein reacts with biuret reagent which indicates 2 or more (A) Blood clotting (B) Peptide bond (C) Disulphide bonds (D) Hydrophobic bonds

Last Answer : Answer : B

Description : Biuret test is specific for (A) Two peptide linkage (B) Phenolic group (C) Imidazole ring (D) None of these

Last Answer : Answer : A

Description : Indole group of tryptophan responses positively to (A) Glyoxylic acid (B) Schiff’s reagent (C) Biuret test (D) Resorcinol test

Last Answer : Answer : A

Description : Group that reacts in the Biuret test: (A) Peptide (B) Amino group (C) Carboxylic group (D) Aldehyde group

Last Answer : Answer : A

Description : Proteins react with biuret reagent which is suggestive of 2 or more (A) Hydrogen bonds (B) Peptide bonds (C) Disulphide bonds (D) Hydrophobic bonds

Last Answer : Answer : B

Description : The useful reagent for detection of amino acids is (A) Molisch reagent (B) Dichlorophenol Indophenol (C) Ninhydrin (D) Biuret

Last Answer : Answer : C

Description : What is the disadvantage of biuret method?

Last Answer : The sensitivity of the method is less and is un- suitable for estimation of proteins in milligram or microgram quantities.

Description : What is the advantage of biuret method?

Last Answer : The biuret method is simple one step process, and is the most widely used method for plasma pro- tein estimations.

Description : What is biuret?

Last Answer : The name is derived from the compound biuret, a condensation product of two urea molecules, which also gives a positive color test.

Description : Will amino acids give a positive biuret test?

Last Answer : No. This needs a minimum of two peptide bonds.

Description : What samples gives a positive test with biuret test?

Last Answer : Biuret test is for peptide bonds.

Description : What does biuret solution specifically react with to produce a color change?

Last Answer : amino acids

Description : What does biuret solution specifically react with to produce a color change?

Last Answer : amino acids

Description : Prilling of urea should be accomplished (in a sprayer) just above the melting point of urea with minimum of retention time, otherwise it will result in (A) Low bulk density product (B) Biuret formation (C) Non-spherical prills (D) Substantially wet non-flowing and sticky product

Last Answer : (B) Biuret formation

Description : __________ is the undesirable by-product produced in the manufacture of urea. (A) Ammonium carbonate (B) Biuret (C) Carbon dioxide (D) Ammonium carbamate

Last Answer : (B) Biuret

Description : Biuret formation in urea is kept at minimum (< 1 %), because it is (A) Corrosive in nature (B) Toxic and harmful to some crops (C) Helpful in decomposition of urea (D) Explosive in nature

Last Answer : (B) Toxic and harmful to some crops

Description : . Chemical formula of biuret is (A) NH2 .CO.NH2 (B) NH3 .COO.NH3 (C) NH2CONHCONH2 (D) NH4COONH2

Last Answer : (C) NH2CONHCONH2

Description : Dehydration of ammonium carbamate produces (A) Urea (B) Biuret (C) Ammonia water (D) None of these

Last Answer : (A) Urea

Description : In the manufacture of urea, the intermediate chemical formed is (A) Biuret (B) Ammonium carbamate (C) Ammonium carbonate (D) None of these

Last Answer : (B) Ammonium carbamat

Description : Lower temperature and large excess of ammonia in urea melt (A) Increases biuret formation (B) Decreases biuret formation (C) Is undesirable (D) Does not effect biuret formation

Last Answer : (B) Decreases biuret formation

Description : Yield of urea can be increased with excess ammonia and higher pressure & temperature, but because of __________ this is normally not done. (A) Increased biuret formation (B) High corrosion rate (C) Increased cost of equipment (D) All (A), (B) & (C)

Last Answer : (D) All (A), (B) & (C)

Description : With increases in pressure, the conversion of Ammonium carbamate into urea (A) Increases (B) Decreases (C) Remains unaltered (D) Can either increase or decrease depends on biuret content

Last Answer : (A) Increases

Description : A protein solution on warming with concentrated HNO3 may turn yellow. This test is called : (a) Xanthoproteic test (b) Ninhydrin test (c) Biuret test (d) Million's test

Last Answer : Xanthoproteic test

Description : Which of the following tests is not used for testing proteins? (a) Ninhydrin test (b) Biuret test (c) Xanthoproteic test (d) Tollens' test

Last Answer : Tollens' test

Description : What happens when urea is treated with hydrazine? (a) Biuret is formed (b) Semicarbazide is formed (c) Carbon dioxide and nitrogen is formed (d) Acetamide is formed

Last Answer : Semicarbazide is formed

Description : Describe the following points with respect to manufacturing of Urea. (i) Temperature (ii) Pressure (iii) Formation of Biuret

Last Answer : Manufacturing of Urea w.r.t following points 1. Temperature: Urea production rate increases as temperature increases up to 180oC and then fall sharply. 2. Pressure: Urea production rate increases as pressure ... before sending to prilling tower. 2NH2 .CO.NH2 = NH2.CO.NH.CO.NH2 + NH