What is the disadvantage of biuret method?

What is the disadvantage of biuret method?
by

1 Answer

Answer :

The sensitivity of the method is less and is un-

suitable for estimation of proteins in milligram

or microgram quantities.
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Related questions

What is the advantage of biuret method?

Description : What is the advantage of biuret method?

Last Answer : The biuret method is simple one step process, and is the most widely used method for plasma pro- tein estimations.

A protein reacts with biuret reagent which indicates 2 or more (A) Blood clotting (B) Peptide bond (C) Disulphide bonds (D) Hydrophobic bonds

Description : A protein reacts with biuret reagent which indicates 2 or more (A) Blood clotting (B) Peptide bond (C) Disulphide bonds (D) Hydrophobic bonds

Last Answer : Answer : B

Biuret test is specific for (A) Two peptide linkage (B) Phenolic group (C) Imidazole ring (D) None of these

Description : Biuret test is specific for (A) Two peptide linkage (B) Phenolic group (C) Imidazole ring (D) None of these

Last Answer : Answer : A

Indole group of tryptophan responses positively to (A) Glyoxylic acid (B) Schiff’s reagent (C) Biuret test (D) Resorcinol test

Description : Indole group of tryptophan responses positively to (A) Glyoxylic acid (B) Schiff’s reagent (C) Biuret test (D) Resorcinol test

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Group that reacts in the Biuret test: (A) Peptide (B) Amino group (C) Carboxylic group (D) Aldehyde group

Description : Group that reacts in the Biuret test: (A) Peptide (B) Amino group (C) Carboxylic group (D) Aldehyde group

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Proteins react with biuret reagent which is suggestive of 2 or more (A) Hydrogen bonds (B) Peptide bonds (C) Disulphide bonds (D) Hydrophobic bonds

Description : Proteins react with biuret reagent which is suggestive of 2 or more (A) Hydrogen bonds (B) Peptide bonds (C) Disulphide bonds (D) Hydrophobic bonds

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The useful reagent for detection of amino acids is (A) Molisch reagent (B) Dichlorophenol Indophenol (C) Ninhydrin (D) Biuret

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Biuret reaction is specific for (A) –CONH-linkages (B) –CSNH2 group (C) –(NH)NH2 group (D) All of these

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What is biuret?

Description : What is biuret?

Last Answer : The name is derived from the compound biuret, a condensation product of two urea molecules, which also gives a positive color test.

What is the use of biuret reaction?

Description : What is the use of biuret reaction? 

Last Answer : This reaction can be used for qualitative identifi- cation and quantitative estimation of proteins.

Will amino acids give a positive biuret test?

Description : Will amino acids give a positive biuret test?

Last Answer : No. This needs a minimum of two peptide bonds.

What is biuret reaction?

Description : What is biuret reaction?

Last Answer : Cupric ions in alkaline medium form a violet colour with peptide bond nitrogen.

What samples gives a positive test with biuret test?

Description : What samples gives a positive test with biuret test?

Last Answer : Biuret test is for peptide bonds.

What does biuret solution specifically react with to produce a color change?

Description : What does biuret solution specifically react with to produce a color change?

Last Answer : amino acids

What are the Reaction of amino acid using ninhydrin and biuret reagent?

Description : What are the Reaction of amino acid using ninhydrin and biuret reagent?

Last Answer : What is the answer ?

What does biuret solution specifically react with to produce a color change?

Description : What does biuret solution specifically react with to produce a color change?

Last Answer : amino acids

Prilling of urea should be accomplished (in a sprayer) just above the melting point of urea with minimum of retention time, otherwise it will result in (A) Low bulk density product (B) Biuret formation (C) Non-spherical prills (D) Substantially wet non-flowing and sticky product

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Last Answer : (B) Biuret formation

__________ is the undesirable by-product produced in the manufacture of urea. (A) Ammonium carbonate (B) Biuret (C) Carbon dioxide (D) Ammonium carbamate

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Pick out the correct statement. (A) Reaction of NH3 with HNO3 to produce (NH4 )2NO3 is endothermic (B) With increase in NH3 /CO2 ratio, urea yield decreases for a given temperature, pressure and total feed rate (C) Biuret (an intermediate during urea manufacture) is toxic to seeds and animals (D) Both (B) and (C)

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Biuret formation in urea is kept at minimum (< 1 %), because it is (A) Corrosive in nature (B) Toxic and harmful to some crops (C) Helpful in decomposition of urea (D) Explosive in nature

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. Chemical formula of biuret is (A) NH2 .CO.NH2 (B) NH3 .COO.NH3 (C) NH2CONHCONH2 (D) NH4COONH2

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Lower temperature and large excess of ammonia in urea melt (A) Increases biuret formation (B) Decreases biuret formation (C) Is undesirable (D) Does not effect biuret formation

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With increases in pressure, the conversion of Ammonium carbamate into urea (A) Increases (B) Decreases (C) Remains unaltered (D) Can either increase or decrease depends on biuret content

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