The DNA fragments produced by the use of restriction endonucleases can be separated by

4. The DNA fragments produced by the use of restriction endonucleases can be separated by
(a) polymerase chain reaction
(b) gel electrophoresis
(c) density gradient centrifugation
(d) any of the above.
by

2 Answers

Answer :

4. (b)gel electrophoresis
by

Answer :

gel electrophoresis
by

Related questions

The normal function of restriction endonucleases is to (A) Excise introns from hrRNA (B) Polymerize nucleotides to form RNA (C) Remove primer from okazaki fragments (D) Protect bacteria from foreign DNA

Description : The normal function of restriction endonucleases is to (A) Excise introns from hrRNA (B) Polymerize nucleotides to form RNA (C) Remove primer from okazaki fragments (D) Protect bacteria from foreign DNA

Last Answer : Answer : D

Restriction endonucleases (A) Cut RNA chains at specific locations (B) Excise introns from hnRNA (C) Remove Okazaki fragments (D) Act as defensive enzymes to protect the host bacterial DNA from DNA of foreign organisms

Description : Restriction endonucleases (A) Cut RNA chains at specific locations (B) Excise introns from hnRNA (C) Remove Okazaki fragments (D) Act as defensive enzymes to protect the host bacterial DNA from DNA of foreign organisms

Last Answer : Answer : D

DNA fragments generated by the restriction endo- nucleases in a chemical reaction can be separated by (a) electrophoresis (b) restriction mapping (c) centrifugation (d) polymerase chain reaction.

Description : DNA fragments generated by the restriction endo- nucleases in a chemical reaction can be separated by (a) electrophoresis (b) restriction mapping (c) centrifugation (d) polymerase chain reaction.

Last Answer : (a) electrophoresis

In sticky ends produced by restriction endonucleases (A) The 2 strands of DNA are joined to each other (B) The DNA strands stick to the restriction endonuclease (C) The ends of a double stranded fragment are overlapping (D) The ends of a double stranded fragment are non overlapping

Description : In sticky ends produced by restriction endonucleases (A) The 2 strands of DNA are joined to each other (B) The DNA strands stick to the restriction endonuclease (C) The ends of a double stranded fragment are overlapping (D) The ends of a double stranded fragment are non overlapping

Last Answer : Answer : C

All of the following statements about restriction endonucleases are true except: (A) They are present in bacteria (B) They act on double stranded DNA (C) They recognize palindromic sequences (D) They always produce sticky ends

Description : All of the following statements about restriction endonucleases are true except: (A) They are present in bacteria (B) They act on double stranded DNA (C) They recognize palindromic sequences (D) They always produce sticky ends

Last Answer : Answer : D

Restriction endonucleases can recognise (A) Palindromic sequences (B) Chimeric DNA (C) DNA-RNA hybrids (D) Homopolymer sequences

Description : Restriction endonucleases can recognise (A) Palindromic sequences (B) Chimeric DNA (C) DNA-RNA hybrids (D) Homopolymer sequences

Last Answer : Answer : A

Restriction endonucleases split (A) RNA (B) Single stranded DNA (C) Double stranded DNA (D) DNA-RNA hybrids

Description : Restriction endonucleases split (A) RNA (B) Single stranded DNA (C) Double stranded DNA (D) DNA-RNA hybrids

Last Answer : Answer : C

Restriction endonucleases recognize and cut a certain sequence of (A) Single stranded DNA (B) Double stranded DNA (C) RNA (D) Protein

Description : Restriction endonucleases recognize and cut a certain sequence of (A) Single stranded DNA (B) Double stranded DNA (C) RNA (D) Protein

Last Answer : Answer : B

Restriction endonucleases are (a) used for in vitro DNA synthesis (b) used in genetic engineering (c) synthesized by bacteria (d) present in mammalian cells for degradation of DNA.

Description : Restriction endonucleases are (a) used for in vitro DNA synthesis (b) used in genetic engineering (c) synthesized by bacteria (d) present in mammalian cells for degradation of DNA.

Last Answer : (b) used in genetic engineering

Restriction endonucleases (a) are present in mammalian cells for degradation of DNA when the cell dies (b) are used in genetic engineering for ligating two DNA molecules (c) are used for in vitro DNA synthesis (d) are synthesized by bacteria as part of their defense mechanism.

Description : Restriction endonucleases (a) are present in mammalian cells for degradation of DNA when the cell dies (b) are used in genetic engineering for ligating two DNA molecules (c) are used for in vitro DNA synthesis (d) are synthesized by bacteria as part of their defense mechanism.

Last Answer : (d) are synthesized by bacteria as part of their defense mechanism.

Restriction endonucleases are enzymes which (a) make cuts at specific positions within the DNA molecule (b) recognize a specific nucleotide sequence for binding of DNA ligase (c) restrict the action of the enzyme DNA polymerase (d) remove nucleotides from the ends of the DNA molecule

Description : Restriction endonucleases are enzymes which (a) make cuts at specific positions within the DNA molecule (b) recognize a specific nucleotide sequence for binding of DNA ligase (c) restrict the action of the enzyme DNA polymerase (d) remove nucleotides from the ends of the DNA molecule

Last Answer : (a) make cuts at specific positions within the DNA molecule

Genetic engineering is possible, because (a) we can cut DNA at specific sites by endonucleases like DNase I (b) restriction endonucleases purified from bacteria can be used in vitro (c) the phenomenon of transduction in bacteria is well understood (d) we can see DNA by electron microscope

Description : Genetic engineering is possible, because (a) we can cut DNA at specific sites by endonucleases like DNase I (b) restriction endonucleases purified from bacteria can be used in vitro (c) the phenomenon of transduction in bacteria is well understood (d) we can see DNA by electron microscope

Last Answer : (b) restriction endonucleases purified from bacteria can be used in vitro

he molecular scissors use3d to cut DNA into specific genes of interest are called (a) Exonucleases (b) Restriction endonucleases (c) Ligases (d) Polymerases

Description : he molecular scissors use3d to cut DNA into specific genes of interest are called (a) Exonucleases (b) Restriction endonucleases (c) Ligases (d) Polymerases

Last Answer : (b) Restriction endonucleases

Without restriction endonucleases, it would be very difficult to a. Force Plasmids into Bacteria. b. Chemically Open Dna Molecules. c. Replicate Dna In A Recombinant Cell. d. Bring About Mutations In Bacteria.

Description : Without restriction endonucleases, it would be very difficult to a. Force Plasmids into Bacteria. b. Chemically Open Dna Molecules. c. Replicate Dna In A Recombinant Cell. d. Bring About Mutations In Bacteria.

Last Answer : b. Chemically Open Dna Molecules.

The restriction enzymes are used in genetic engineering, because (a) they can cut DNA at specific base sequence (b) they are nucleases that cut DNA at variable sites (c) they can degrade harmful proteins (d) they can join different DNA fragments.

Description : The restriction enzymes are used in genetic engineering, because (a) they can cut DNA at specific base sequence (b) they are nucleases that cut DNA at variable sites (c) they can degrade harmful proteins (d) they can join different DNA fragments.

Last Answer : (a) they can cut DNA at specific base sequence

Do eukaryotic cells have restriction endonucleases? -Biology

Description : Do eukaryotic cells have restriction endonucleases? -Biology

Last Answer : answer:

Restriction endonucleases are present in (A) Viruses (B) Bacteria (C) Eukaryotes (D) All of these

Description : Restriction endonucleases are present in (A) Viruses (B) Bacteria (C) Eukaryotes (D) All of these

Last Answer : Answer : B

The DNA fragments separated on an agarose gel can be visualised after staining with (a) acetocarmine (b) aniline blue (c) ethidium bromide (d) bromophenol blue.

Description : The DNA fragments separated on an agarose gel can be visualised after staining with (a) acetocarmine (b) aniline blue (c) ethidium bromide (d) bromophenol blue.

Last Answer : (c) ethidium bromide

In gel electrophoresis, separated DNA fragments can be visualized with the help of (a) acetocarmine in bright blue light (b) ethidium bromide in UV radiation (c) acetocarmine in UV radiation (d) ethidium bromide in infrared radiation.

Description : In gel electrophoresis, separated DNA fragments can be visualized with the help of (a) acetocarmine in bright blue light (b) ethidium bromide in UV radiation (c) acetocarmine in UV radiation (d) ethidium bromide in infrared radiation.

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The DNA fragments separated on an agarose gel can be visualised after staining with : (1) Acetocarmine (2) Aniline blue (3) Ethidium bromide (4) Bromophenol blue

Description : The DNA fragments separated on an agarose gel can be visualised after staining with : (1) Acetocarmine (2) Aniline blue (3) Ethidium bromide (4) Bromophenol blue

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What are the restriction fragments? -Biology

Description : What are the restriction fragments? -Biology

Last Answer : answer:

The linking of antibiotic resistance gene with the plasmid vector became possible with (a) DNA polymerase (b) exonucleases (c) DNA ligase (d) endonucleases.

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Read the following four statements (A-D) about certain mistakes in two of them. (A) The first transgenic buffalo, Rosie produced milk which was human alpha-lactalbumin enriched. (B) Restriction enzymes are used in isolation of DNA from other macromolecules. (C) Downstream processing is one of the steps of rDNA technology. (D) Disarmed pathogen vectors are also used in transfer of rDNA into the host. Which of the two statements have mistakes? (a) B and C (b) C and D (c) A and C (d) A and B

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What is the technique used for separation of DNA fragments. -Biology

Description : What is the technique used for separation of DNA fragments. -Biology

Last Answer : answer:

How does gel electrophoresis separate DNA fragments?

Description : How does gel electrophoresis separate DNA fragments?

Last Answer : There is a specific density of gel used in the electrophoresis.The DNA is placed in a well, and then electrical charge is used topull the DNA through the gel. Because spliced DNA is ... taxi cab, which is larger, movesslower through the traffic. The bicycle which is smaller, movesquicker.

A scientist uses materials in her lab to cut a dna sample into fragments what process is the scientist using?

Description : A scientist uses materials in her lab to cut a dna sample into fragments what process is the scientist using?

Last Answer : PCR or polymerase chain reaction. :: Apex

Why can gel electrophoresis separate dna fragments?

Description : Why can gel electrophoresis separate dna fragments?

Last Answer : Electrophoresis enables you to distinguish DNA fragments of different lengths. DNA is negatively charged, therefore, when an electric current is applied to the gel, DNA will migrate towards the positively ... marker, you can work out the approximate length of the DNA fragments in the samples.

Why can gel electrophoresis separate dna fragments?

Description : Why can gel electrophoresis separate dna fragments?

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Okasaki fragments are small bits of (A) RNA (B) DNA (C) DNA with RNA heads (D) RNA with DNA heads

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Lambda phage can be used to clone DNA fragments of the size (A) Upto 3 kilobases (B) Upto 20 kilobases (C) Upto 45 kilobases (D) Upto 1,000 kilobases

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Fragments of DNA can be identified by the technique of (A) Western blotting (B) Eastern blotting (C) Northern blotting (D) Southern blotting

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DNA fragments are sealed by (A) DNA polymerase II (B) DNA ligase (C) DNA gyrase (D) DNA topoisomerase II

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On what basis the short fragments of DNA arrange themselves?

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Gel electrophoresis is used for (a) construction of recombinant DNA by joining with cloning vectors (b) isolation of DNA molecules (c) cutting of DNA into fragments (d) separation of DNA fragments according to their size.

Description : Gel electrophoresis is used for (a) construction of recombinant DNA by joining with cloning vectors (b) isolation of DNA molecules (c) cutting of DNA into fragments (d) separation of DNA fragments according to their size.

Last Answer : (d) separation of DNA fragments according to their size.

What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis ? (a) The smaller the fragment size, the farther it moves. (b) Positively charged fragments move to farther end. (c) Negatively charged fragments do not move. (d) The larger the fragment size, the farther it moves.

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DNA fragments are (a) negatively charged (b) neutral (c) either positively or negatively charged depending on their size (d) positively charged

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During DNA replication, Okazaki fragments are used to elongate (a) the lagging strand towards replication fork (b) the leading strand away from replication fork (c) the lagging strand away from the replication fork (d) the leading strand towards replication fork.

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Competence refers to a bacterial cell's ability to a. Take Up DNA Fragments From The Environment. b. Be Infected By A Transducing Phage. c. Produce A Protein Product From An Engineered Gene. d. Undergo Conjugation With An F+ Cell.

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Transformation refers to a. Using A Virus To Transfer DNA Fragments. b. DNA Fragments Transferred Between Live Donor And Recipient Cells. c. The Formation of an F- Recombinant Cell. d. The Transfer of Naked Fragments of DNA

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Last Answer : b. DNA Fragments Transferred Between Live Donor And Recipient Cells.

In the semiconservative method of replication a. Both parent strands are degraded. b. One parent strand is conserved in each of the the new dna. c. Both parent strands reform with one another. d. Okazaki fragments form both of the new molecules of DNA.

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Last Answer : b. One parent strand is conserved in each of the the new dna.

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Last Answer : d. Okazaki fragments; DNA ligase

In DNA sequencing, the primer A.specifies where the sequence ends B.specifies where the sequence begins C.both (a) and (b) D.generates variety of different sized fragments

Description : In DNA sequencing, the primer A.specifies where the sequence ends B.specifies where the sequence begins C.both (a) and (b) D.generates variety of different sized fragments

Last Answer : B.specifies where the sequence begins

During DNA replication, Okazaki fragments are used to elongate: (1) The lagging strand towards replication fork. (2) The leading strand away from replication fork. (3) The lagging strand away from the replication fork. (4) The leading strand towards replication fork.

Description : During DNA replication, Okazaki fragments are used to elongate: (1) The lagging strand towards replication fork. (2) The leading strand away from replication fork. (3) The lagging strand away from the replication fork. (4) The leading strand towards replication fork.

Last Answer : (3) The lagging strand away from the replication fork.

DNA fragments are: (1) Negatively charged (2) Neutral (3) Either positively or negatively charged depending on their size (4) Positively charged

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Last Answer : (1) Negatively charged

Platelets are the cell fragments produced from

Description : Platelets are the cell fragments produced from

Last Answer : Platelets are the cell fragments produced from A. Thrombocytes B. Megakaryocytes C. Monocytes D. Mast cell

How are restriction enzymes used to make recombinant DNA? -Biology

Description : How are restriction enzymes used to make recombinant DNA? -Biology

Last Answer : answer:

Role of restriction enzymes and DNA ligase -Biology

Description : Role of restriction enzymes and DNA ligase -Biology

Last Answer : answer: